An undergraduate lecture on immunologic tolerance, it's various types and how a breakdown of tolerance contributes to the pathogenesis of autoimmune diseases. Additionally a small quiz at the end to gauge the students' learning.
An undergraduate lecture on immunologic tolerance, it's various types and how a breakdown of tolerance contributes to the pathogenesis of autoimmune diseases. Additionally a small quiz at the end to gauge the students' learning.
introduction of adaptive immunity. classification of adaptive immunity, factor affecting it and mechanism of adaptive immunity comparison between adaptive immunity and innate immunity. characteristic of adaptive immunity . cell mediated immune responses immunoglobulins
types of immunoglobulins. functions of immunoglobulins, hypersensitivity reactions
T cells are one of the important white blood cells of the immune system and play a central role in the adaptive immune response and are distinguished from other lymphocytes by the presence of a T-cell receptor (TCR) on their cell surface.
B cells, also known as B lymphocytes, are a type of white blood cell of the lymphocyte subtype. They function in the humoral immunity component of the adaptive immune system.. B cells produce antibody molecules.
In mammals, B cells mature in the bone marrow, which is at the core of most bones. In birds, B cells mature in the bursa of Fabricus.
B cells present antigens (they are also classified as professional antigen-presenting cells (APCs)) and secrete cytokines.
Type II Hypersensitivity-Antibody mediated cytotoxic HypersensitivityAnup Bajracharya
Type II Hypersensitivity is antibody-mediated immune reaction in which antibodies (IgG or IgM) are directed against cellular or extracellular matrix antigens with the resultant cellular destruction, functional loss, or damage to tissues.
introduction of adaptive immunity. classification of adaptive immunity, factor affecting it and mechanism of adaptive immunity comparison between adaptive immunity and innate immunity. characteristic of adaptive immunity . cell mediated immune responses immunoglobulins
types of immunoglobulins. functions of immunoglobulins, hypersensitivity reactions
T cells are one of the important white blood cells of the immune system and play a central role in the adaptive immune response and are distinguished from other lymphocytes by the presence of a T-cell receptor (TCR) on their cell surface.
B cells, also known as B lymphocytes, are a type of white blood cell of the lymphocyte subtype. They function in the humoral immunity component of the adaptive immune system.. B cells produce antibody molecules.
In mammals, B cells mature in the bone marrow, which is at the core of most bones. In birds, B cells mature in the bursa of Fabricus.
B cells present antigens (they are also classified as professional antigen-presenting cells (APCs)) and secrete cytokines.
Type II Hypersensitivity-Antibody mediated cytotoxic HypersensitivityAnup Bajracharya
Type II Hypersensitivity is antibody-mediated immune reaction in which antibodies (IgG or IgM) are directed against cellular or extracellular matrix antigens with the resultant cellular destruction, functional loss, or damage to tissues.
immunological tolerance can be divided into two parts. they are central tolerance and peripheral tolerance. this slide contains information on development of central tolerance which include both B cell and T cell central tolerance.
Kell blood group system most important blood group system following to ABO and Rh blood group system, particularly RhD as far as immunogenicity is concerned and Its clinical importance.
What is antigen
What is epitope & paratope?
Classification of antigen
Pro antigen
Superantigens
Antigenicity
Determinants of antigenicity
Test for antigen detection
Autoimmunity and autoimmune diseases dr. ihsan alsaimarydr.Ihsan alsaimary
Dr. ihsan edan abdulkareem alsaimary
PROFESSOR IN MEDICAL MICROBIOLOGY AND MOLECULAR IMMUNOLOGY
ihsanalsaimary@gmail.com
mobile : 009647801410838
university of basrah - college of medicine - basrah -IRAQ
Julius Donath and Karl Landsteiner (1904)reported autoantibodies can cause disease by showing that autoantibodies (‘hemolysins’) caused paroxysmal cold hemoglobinuria.
ANTIGEN, HAPTEN, ALL TYPES OF ANTIGENS, IMMUNOGEN , ATTRIBUTES OF ANTIGENICITY, DETERMINANTS OF ANTIGENICITY,
IMMUNOLOGY KUBY, MEDICAL MICROBIOLOGY & IMMUNOLOGY OF PANIKER , LIPPINCOTT'S IMMUNOLOGY, OTHER SOURCES.
The Roman Empire A Historical Colossus.pdfkaushalkr1407
The Roman Empire, a vast and enduring power, stands as one of history's most remarkable civilizations, leaving an indelible imprint on the world. It emerged from the Roman Republic, transitioning into an imperial powerhouse under the leadership of Augustus Caesar in 27 BCE. This transformation marked the beginning of an era defined by unprecedented territorial expansion, architectural marvels, and profound cultural influence.
The empire's roots lie in the city of Rome, founded, according to legend, by Romulus in 753 BCE. Over centuries, Rome evolved from a small settlement to a formidable republic, characterized by a complex political system with elected officials and checks on power. However, internal strife, class conflicts, and military ambitions paved the way for the end of the Republic. Julius Caesar’s dictatorship and subsequent assassination in 44 BCE created a power vacuum, leading to a civil war. Octavian, later Augustus, emerged victorious, heralding the Roman Empire’s birth.
Under Augustus, the empire experienced the Pax Romana, a 200-year period of relative peace and stability. Augustus reformed the military, established efficient administrative systems, and initiated grand construction projects. The empire's borders expanded, encompassing territories from Britain to Egypt and from Spain to the Euphrates. Roman legions, renowned for their discipline and engineering prowess, secured and maintained these vast territories, building roads, fortifications, and cities that facilitated control and integration.
The Roman Empire’s society was hierarchical, with a rigid class system. At the top were the patricians, wealthy elites who held significant political power. Below them were the plebeians, free citizens with limited political influence, and the vast numbers of slaves who formed the backbone of the economy. The family unit was central, governed by the paterfamilias, the male head who held absolute authority.
Culturally, the Romans were eclectic, absorbing and adapting elements from the civilizations they encountered, particularly the Greeks. Roman art, literature, and philosophy reflected this synthesis, creating a rich cultural tapestry. Latin, the Roman language, became the lingua franca of the Western world, influencing numerous modern languages.
Roman architecture and engineering achievements were monumental. They perfected the arch, vault, and dome, constructing enduring structures like the Colosseum, Pantheon, and aqueducts. These engineering marvels not only showcased Roman ingenuity but also served practical purposes, from public entertainment to water supply.
Operation “Blue Star” is the only event in the history of Independent India where the state went into war with its own people. Even after about 40 years it is not clear if it was culmination of states anger over people of the region, a political game of power or start of dictatorial chapter in the democratic setup.
The people of Punjab felt alienated from main stream due to denial of their just demands during a long democratic struggle since independence. As it happen all over the word, it led to militant struggle with great loss of lives of military, police and civilian personnel. Killing of Indira Gandhi and massacre of innocent Sikhs in Delhi and other India cities was also associated with this movement.
June 3, 2024 Anti-Semitism Letter Sent to MIT President Kornbluth and MIT Cor...Levi Shapiro
Letter from the Congress of the United States regarding Anti-Semitism sent June 3rd to MIT President Sally Kornbluth, MIT Corp Chair, Mark Gorenberg
Dear Dr. Kornbluth and Mr. Gorenberg,
The US House of Representatives is deeply concerned by ongoing and pervasive acts of antisemitic
harassment and intimidation at the Massachusetts Institute of Technology (MIT). Failing to act decisively to ensure a safe learning environment for all students would be a grave dereliction of your responsibilities as President of MIT and Chair of the MIT Corporation.
This Congress will not stand idly by and allow an environment hostile to Jewish students to persist. The House believes that your institution is in violation of Title VI of the Civil Rights Act, and the inability or
unwillingness to rectify this violation through action requires accountability.
Postsecondary education is a unique opportunity for students to learn and have their ideas and beliefs challenged. However, universities receiving hundreds of millions of federal funds annually have denied
students that opportunity and have been hijacked to become venues for the promotion of terrorism, antisemitic harassment and intimidation, unlawful encampments, and in some cases, assaults and riots.
The House of Representatives will not countenance the use of federal funds to indoctrinate students into hateful, antisemitic, anti-American supporters of terrorism. Investigations into campus antisemitism by the Committee on Education and the Workforce and the Committee on Ways and Means have been expanded into a Congress-wide probe across all relevant jurisdictions to address this national crisis. The undersigned Committees will conduct oversight into the use of federal funds at MIT and its learning environment under authorities granted to each Committee.
• The Committee on Education and the Workforce has been investigating your institution since December 7, 2023. The Committee has broad jurisdiction over postsecondary education, including its compliance with Title VI of the Civil Rights Act, campus safety concerns over disruptions to the learning environment, and the awarding of federal student aid under the Higher Education Act.
• The Committee on Oversight and Accountability is investigating the sources of funding and other support flowing to groups espousing pro-Hamas propaganda and engaged in antisemitic harassment and intimidation of students. The Committee on Oversight and Accountability is the principal oversight committee of the US House of Representatives and has broad authority to investigate “any matter” at “any time” under House Rule X.
• The Committee on Ways and Means has been investigating several universities since November 15, 2023, when the Committee held a hearing entitled From Ivory Towers to Dark Corners: Investigating the Nexus Between Antisemitism, Tax-Exempt Universities, and Terror Financing. The Committee followed the hearing with letters to those institutions on January 10, 202
Biological screening of herbal drugs: Introduction and Need for
Phyto-Pharmacological Screening, New Strategies for evaluating
Natural Products, In vitro evaluation techniques for Antioxidants, Antimicrobial and Anticancer drugs. In vivo evaluation techniques
for Anti-inflammatory, Antiulcer, Anticancer, Wound healing, Antidiabetic, Hepatoprotective, Cardio protective, Diuretics and
Antifertility, Toxicity studies as per OECD guidelines
The French Revolution, which began in 1789, was a period of radical social and political upheaval in France. It marked the decline of absolute monarchies, the rise of secular and democratic republics, and the eventual rise of Napoleon Bonaparte. This revolutionary period is crucial in understanding the transition from feudalism to modernity in Europe.
For more information, visit-www.vavaclasses.com
2024.06.01 Introducing a competency framework for languag learning materials ...Sandy Millin
http://sandymillin.wordpress.com/iateflwebinar2024
Published classroom materials form the basis of syllabuses, drive teacher professional development, and have a potentially huge influence on learners, teachers and education systems. All teachers also create their own materials, whether a few sentences on a blackboard, a highly-structured fully-realised online course, or anything in between. Despite this, the knowledge and skills needed to create effective language learning materials are rarely part of teacher training, and are mostly learnt by trial and error.
Knowledge and skills frameworks, generally called competency frameworks, for ELT teachers, trainers and managers have existed for a few years now. However, until I created one for my MA dissertation, there wasn’t one drawing together what we need to know and do to be able to effectively produce language learning materials.
This webinar will introduce you to my framework, highlighting the key competencies I identified from my research. It will also show how anybody involved in language teaching (any language, not just English!), teacher training, managing schools or developing language learning materials can benefit from using the framework.
A Strategic Approach: GenAI in EducationPeter Windle
Artificial Intelligence (AI) technologies such as Generative AI, Image Generators and Large Language Models have had a dramatic impact on teaching, learning and assessment over the past 18 months. The most immediate threat AI posed was to Academic Integrity with Higher Education Institutes (HEIs) focusing their efforts on combating the use of GenAI in assessment. Guidelines were developed for staff and students, policies put in place too. Innovative educators have forged paths in the use of Generative AI for teaching, learning and assessments leading to pockets of transformation springing up across HEIs, often with little or no top-down guidance, support or direction.
This Gasta posits a strategic approach to integrating AI into HEIs to prepare staff, students and the curriculum for an evolving world and workplace. We will highlight the advantages of working with these technologies beyond the realm of teaching, learning and assessment by considering prompt engineering skills, industry impact, curriculum changes, and the need for staff upskilling. In contrast, not engaging strategically with Generative AI poses risks, including falling behind peers, missed opportunities and failing to ensure our graduates remain employable. The rapid evolution of AI technologies necessitates a proactive and strategic approach if we are to remain relevant.
Model Attribute Check Company Auto PropertyCeline George
In Odoo, the multi-company feature allows you to manage multiple companies within a single Odoo database instance. Each company can have its own configurations while still sharing common resources such as products, customers, and suppliers.
3. Tolerance
Our own bodies produce some 100,000 different
proteins and one of the longstanding conundrums of
immunology has been to understand how the
immune system produces a virtual repertoire
against pathogens while at the same time avoiding
reacting to self.
The strict definition of immunological tolerance
occurs when an immunocompetent host fails to
respond to an immunogenic challenge with a
specific antigen.
5. Tolerance
Definition:
Immunological non-reactivity to an antigen.
Resulting from a previous exposure.
The most important form is non-reactivity to self Ag
When an antigen induces tolerance, it is tolerogen.
6.
7. Tolerance
Immune response Tolerance
Physical form of Ag Large,aggregated, complex Soluble, smaller, less complex
Route of Ag SC or IM Oral or IV
Dose of Ag Optimal dose Very large (sometimes very
small)
Age of responding Older and immunologically Newborn, immunologicall
animal mature immature
Differentiation state Fully differentiated; memory Relatively undifferen: B, T cells
of cells T and B
8. Tolerance, mechanism
B cell tolerance
• Deletion
• Anergy
• receptor editing
T cell tolerance
• Deletion
• Ignorance
Loss of Ts cells
Anti-idiotype antibody
9.
10. Tolerance, T cell tolerance
Thymus
• Positive selection: cells that are able to recognize
and bind to self MHC or to peptide + MHC molecules
are selected to grow
• Negative selection: cells that recognize and
efficiently bind self peptides are auto-reactive cells
and undergo apoptotic cell death because they are
harmful to the host
Cells that pass both positive and negative selection
tests “graduate” from thymus ; enter circulation as
mature T lymphocytes
11. Mechanism of tolerance
1- Clonal deletion:
Auto-reactive T-cells are eliminated in the thymus
following interaction with self-antigen during their
differentiation (negative selection).
Likewise, differentiating early B cells become
tolerant when they encounter cell-associated or
soluble self-antigen.
12. Mechanism of tolerance
2- Clonal anergy:
Auto-reactive T cells, when exposed to antigenic
peptides lose the second signal, become anergic to
the antigen.
B cells when exposed to large amounts of soluble
antigen down regulate their surface IgM and
become anergic.
14. Ignorance
It can be shown that there are T cells and B cells
specific for auto-antigens present in circulation.
These cells are quite capable of making a
response but are unaware of the presence of their
auto-antigen. This arises for 2 reasons.
15. Ignorance
The first is that the antigen may simply be present
in too low concentration. Since all lymphocytes
have a threshold for receptor occupancy which is
required to trigger a response then very low
concentrations of antigen will not be sensed.
16. Ignorance
The second possibility is a more interesting one.
Some antigens are sequestered from the immune
system in locations which are not freely exposed
to surveillance.
These are termed immunologically privileged
sites. Examples of such sites are the eye, CNS
and testis.
Pathologically mediated disruption of these
privileged sites may expose the sequestered
antigens leading to an autoimmune response.
17. Mechanism of tolerance
4- Receptor editing:
B cells which encounter large amounts of soluble
antigen, as they do in the body, and bind to this
antigen with very low affinity become activated to
re-express their RAG-1 and RAG-2 genes.
These genes cause them to undergo DNA
recombination and change their antigen
specificity.
18.
19. Mechanism of tolerance
5- Anti-idiotype antibody:
produced during the process of tolerization. They
prevent the receptor from combining with antigen
so inhibit immune response to it.
6- Suppressor cells:
Both low and high doses of antigen may induce
suppressor T cells, which can specifically suppress
immune responses of both B and T cells.
22. AUTOIMMUNITY- Definition
Immune recognition and injury of self tissues
(autoimmunity) results from a loss of self tolerance.
Autoimmunity is
Breakdown of mechanisms responsible for self
tolerance
Induction of an immune response against components
of the self.
23. Loss of Self Tolerance
Most self peptides are presented at levels
too low to engage effector T cells
those presented at high levels induce clonal
deletion or anergy.
Autoimmunity arises most frequently to
Tissue-specific antigens with only certain MHC
molecules
present the peptide at an intermediate level
recognized by T cells without inducing tolerance.
24. MHC Association with
Autoimmune Disease
The level of presented autoantigenic peptide
Is determined by residues in MHC molecules
These molecules govern the affinity of peptide
binding.
Autoimmune diseases are associated with
particular MHC genotypes.
25. Classification
Table 1. Spectrum of autoimmune diseases, target organs and diagnostic tests
Disease Organ Antibody to Diagnostic Test
Hashimoto's thyroiditis Thyroid Thyroglobulin, thyroid RIA, Passive, CF,
Organ specific
peroxidase hemagglutination
Primary Myxedema Thyroid Cytoplasmic TSH receptor Immunofluorescence (IF)
Pernicious anemia Red cells Intrinsic factor, Gastric B-12 binding to IF
parietal cell immunofluorescence
Addison's disease Adrenal Adrenal cells Immunofluorescence
Male infertility Sperm Spermatozoa Agglutination,
Immunofluorescence
Insulin dependent juvenile Pancreas Pancreatic islet beta cells
diabetes
Insulin resistant diabetic Systemic Insulin receptor Competition for receptor
Myasthenia graves Muscle Muscle, acetyl choline Immunofluorescence,
receptor competition for receptor
Vitiligo Skin Joints Melanocytes Immunofluorescence
Non-organ Rheumatoid arthritis Skin, kidney, IgG IgG-latex agglutination
specific joints
Systemic lupus Joints, etc. DNA, RNA, nucleoproteins RNA-, DNA-latex agglutination,
erythematosus IF
26. Organ-specific Autoimmune
diseases
Antigens and autoimmunity restricted to specific
organs in the body
Hashimoto’ thyroiditis
Type I diabetes
Multiple sclerosis
Grave’s disease
Myasthenia gravis
27. Systemic Autoimmune Disease
Antigens and autoimmunity are distributed in
many tissues (systemic)
Rheumatoid arthritis
polymyositis
Scleroderma
Systemic lupus erythematosus
29. AUTOIMMUNITY- aetiology
1. Sequestered antigen
Lymphoid cells may not be exposed to some self
antigens during their differentiation,
They may be late-developing antigens or may be
confined to specialized organs (e.g., testes, brain,
eye, etc.).
A release of antigens from these organs
resulting from
accidental traumatic injury or
surgery can
Result in the stimulation of an immune response
and initiation of an autoimmune disease.
30. AUTOIMMUNITY- aetiology
1. Escape of auto-reactive clones
The negative selection in the thymus may not be fully
functional to eliminate self reactive cells.
Not all self antigens may be represented in the
thymus
Certain antigens may not be properly processed and
presented.
31. AUTOIMMUNITY- aetiology
1. Cross reactive antigens
Antigens on certain pathogens may have
determinants which cross react with self antigens
and an immune response against these
determinants may lead to effector cell or
antibodies against tissue antigens.
Post streptococcal nephritis and carditis,
anticardiolipin antibodies during syphilis
Association between Klebsiella and ankylosing
spondylitis.
32. AUTOIMMUNITY- aetiology
Infectious triggers:
stimulation of co-stimulatory signals,
inappropriate MHC II expression, or cytokines
Molecular mimicry (cross-reaction)
Release of sequestered antigens
T cell bypass (pathogen binding to self protein)
Superantigen activity/polyclonal activation
34. AUTOIMMUNITY- Diagnosis
Diagnosis:
Clinical
Detection of Ab reactive against soluble antigens
by ELISA.
Detection of Ab against tissues and cells by IF.
In some cases, a biological /biochemical assay
may be used (e.g., Graves diseases, pernicious
anemia).