Dr. ihsan edan abdulkareem alsaimary
PROFESSOR IN MEDICAL MICROBIOLOGY AND MOLECULAR IMMUNOLOGY
ihsanalsaimary@gmail.com
mobile : 009647801410838
university of basrah - college of medicine - basrah -IRAQ
The immune response is how our body recognizes and defends itself against pathogens like bacteria, viruses, and substances that appear foreign and harmful.
This document provides an overview of autoimmune diseases. It discusses how a defect in the immune system can trigger autoimmunity and lists examples of autoimmune disorders like rheumatoid arthritis, Graves' disease, and Hashimoto's thyroiditis. The causes of autoimmunity include genetic susceptibility and environmental triggers like infections. Viruses can induce autoimmunity through molecular mimicry or by damaging tissues and exposing new antigens.
This document summarizes the key components and steps of the immune response. It describes the main cellular components of the immune system including T cells, B cells, natural killer cells, monocytes, macrophages, dendritic cells, and other granular leukocytes. It also discusses immunoglobulins, antigen presentation, opsonization, phagocytosis, and the complement system. The immune response involves recognition of pathogens by immune cells, activation and proliferation of responsive cells, and elimination of the pathogen through actions like phagocytosis, antibody production, and induction of cell death.
An undergraduate lecture on immunologic tolerance, it's various types and how a breakdown of tolerance contributes to the pathogenesis of autoimmune diseases. Additionally a small quiz at the end to gauge the students' learning.
The document discusses various types of tumor antigens recognized by T cells and immune mechanisms of tumor rejection. It covers tumor-associated antigens and tumor-specific antigens, and describes four main categories of tumor antigens: 1) abnormally expressed but unmutated cellular proteins, 2) products of mutated self genes, 3) oncogenes and mutated tumor suppressor genes, and 4) antigens of oncogenic viruses. It also discusses how tumors can evade immune responses through mechanisms like antigen loss, inhibiting immune molecules, regulatory T cells, and secreting immunosuppressive factors. Novel immunotherapies discussed include therapeutic cancer vaccines, monoclonal antibodies, immune checkpoint inhibitors, adoptive cell therapies, and oncolytic viruses.
This document summarizes the cells and organs of the immune system. It describes hematopoiesis, the formation of blood cells from hematopoietic stem cells in the bone marrow. The main immune cells are lymphocytes (B, T, and NK cells), myeloid cells (monocytes, macrophages, neutrophils, eosinophils, basophils, mast cells, dendritic cells), and the organs they develop and function in such as the thymus, bone marrow, lymph nodes, spleen, and mucosal associated lymphoid tissues. The immune cells work together to identify and eliminate pathogens and tumor cells through mechanisms like phagocytosis, antibody production, cytokine signaling, and cytotoxic killing.
IMMUNE RESPONSE TO TUMORS-Humoral immunity
-Cellular Immunity- Failure of Host Defenses
- Evasion of Immune Responses by Tumors
- Cancer Immunosurveillance vs Immunoediting- Immunotherapy
The immune response is how our body recognizes and defends itself against pathogens like bacteria, viruses, and substances that appear foreign and harmful.
This document provides an overview of autoimmune diseases. It discusses how a defect in the immune system can trigger autoimmunity and lists examples of autoimmune disorders like rheumatoid arthritis, Graves' disease, and Hashimoto's thyroiditis. The causes of autoimmunity include genetic susceptibility and environmental triggers like infections. Viruses can induce autoimmunity through molecular mimicry or by damaging tissues and exposing new antigens.
This document summarizes the key components and steps of the immune response. It describes the main cellular components of the immune system including T cells, B cells, natural killer cells, monocytes, macrophages, dendritic cells, and other granular leukocytes. It also discusses immunoglobulins, antigen presentation, opsonization, phagocytosis, and the complement system. The immune response involves recognition of pathogens by immune cells, activation and proliferation of responsive cells, and elimination of the pathogen through actions like phagocytosis, antibody production, and induction of cell death.
An undergraduate lecture on immunologic tolerance, it's various types and how a breakdown of tolerance contributes to the pathogenesis of autoimmune diseases. Additionally a small quiz at the end to gauge the students' learning.
The document discusses various types of tumor antigens recognized by T cells and immune mechanisms of tumor rejection. It covers tumor-associated antigens and tumor-specific antigens, and describes four main categories of tumor antigens: 1) abnormally expressed but unmutated cellular proteins, 2) products of mutated self genes, 3) oncogenes and mutated tumor suppressor genes, and 4) antigens of oncogenic viruses. It also discusses how tumors can evade immune responses through mechanisms like antigen loss, inhibiting immune molecules, regulatory T cells, and secreting immunosuppressive factors. Novel immunotherapies discussed include therapeutic cancer vaccines, monoclonal antibodies, immune checkpoint inhibitors, adoptive cell therapies, and oncolytic viruses.
This document summarizes the cells and organs of the immune system. It describes hematopoiesis, the formation of blood cells from hematopoietic stem cells in the bone marrow. The main immune cells are lymphocytes (B, T, and NK cells), myeloid cells (monocytes, macrophages, neutrophils, eosinophils, basophils, mast cells, dendritic cells), and the organs they develop and function in such as the thymus, bone marrow, lymph nodes, spleen, and mucosal associated lymphoid tissues. The immune cells work together to identify and eliminate pathogens and tumor cells through mechanisms like phagocytosis, antibody production, cytokine signaling, and cytotoxic killing.
IMMUNE RESPONSE TO TUMORS-Humoral immunity
-Cellular Immunity- Failure of Host Defenses
- Evasion of Immune Responses by Tumors
- Cancer Immunosurveillance vs Immunoediting- Immunotherapy
This document discusses autoimmunity and autoimmune diseases. It begins by defining autoimmunity as a breakdown of self-tolerance mechanisms that leads to an adaptive immune response against self-antigens. This can result in chronic inflammation and autoimmune disease. Several proposed mechanisms for how autoimmunity occurs are described, including defects in central and peripheral tolerance. Factors like genetics, hormones, infections, and environmental exposures are thought to contribute to loss of self-tolerance. The document then classifies and describes some examples of organ-specific and systemic autoimmune diseases in more detail.
Hypersensitivity type 3: antibody antigen complexSara Hassan
Hypersensitivity type 3 involves antigen binding to IgG to form immune complexes that circulate in the blood and activate complement. These moderate complexes deposit in basement membranes and cause inflammation by attracting leukocytes, which can lead to anaphylactic shock. Immune complexes trigger inflammatory processes by activating complement anaphylatoxins and stimulating histamine release from basophils and mast cells. Neutrophils are also attracted to sites of immune complex deposition and release enzymes that damage tissues. Treatment involves steroids, interferons, or cyclosporin to reduce inflammation.
Autoimmune DIseases : Types, Mechanism, Diagnosis, TreatmentDr Mehul Dave
This is a presentation useful to learners of immunology as well as acadeicians. Useful in undergraduate as well as postgraduate courses. NEET students/Teachers can also get advantage of it.
This document discusses cancer immunotherapy. It begins by describing tumor antigens that can be recognized by the immune system, such as tumor-specific antigens, tumor-associated antigens, and antigens from oncogenic viruses. It then discusses how tumors evade the immune system and various approaches to immunotherapy, including active immunotherapies using vaccines made of tumor cells, purified antigens, or antigen-loaded dendritic cells. Passive immunotherapies discussed include adoptive cellular therapy, monoclonal antibodies, and immunotoxins. Clinical trials and effectiveness of different immunotherapies are also summarized.
The immune system consists of cells, proteins, and lymphoid organs that work together to protect the body from infection. The immune system has two branches: innate immunity provides a general and immediate response, while adaptive immunity provides a tailored response after initial exposure. Innate immunity involves physical barriers and cells like macrophages that recognize pathogens. Adaptive immunity involves B and T cells that recognize specific pathogens and mount stronger responses upon reexposure. Cytokines are proteins that regulate immune cell growth and activation and mediate inflammatory responses.
Introduction to hypersensitive reactionsDeepika Rana
This document provides an overview of hypersensitive reactions and their classification. It discusses the five main types of hypersensitivity reactions: type I-IV reactions which are antibody-mediated and immediate (types I-III) or delayed (type IV); and type V which involves antibody stimulation of cell surface receptors. Type I is allergy mediated by IgE antibodies. Type II involves IgG/IgM binding to cell surfaces. Type III occurs via immune complex deposition. Type IV is cell-mediated via T cells. Examples of each type are given.
Autoimmunity is caused by the immune system attacking the body's own tissues and organs. Around 5-7% of adults are affected by autoimmune diseases, which are more common in women. Left-handed individuals have a higher risk of autoimmunity. Mechanisms that can trigger autoimmunity include molecular mimicry between foreign and self-antigens, genetic factors that influence antigen presentation, and infections that dysregulate the immune response. Treatment options aim to suppress the immune system or remove autoantibodies.
The document summarizes key concepts in cancer immunology. It defines cancer and how the immune system views cancer cells. It describes how the innate and adaptive immune systems recognize and attack tumors through mechanisms like complement activation, NK cell activation, and T cell responses. It also discusses how cancers can evade the immune system through antigen loss, immunosuppressive microenvironments, and immune checkpoint proteins. The document concludes by outlining several immunotherapy approaches like non-specific immune stimulation, T cell therapy, immune checkpoint inhibitors, cancer vaccines, and monoclonal antibodies.
The major histocompatibility complex (MHC) is a set of surface proteins that present antigens to T cells. MHC molecules are classified into two groups: Class I MHC molecules are expressed on all nucleated cells and present intracellular antigens to CD8+ T cells. Class II MHC molecules are mainly expressed on antigen-presenting cells and present extracellular antigens to CD4+ T cells. MHC molecules play an important role in organ transplantation by determining tissue compatibility and in the immune response by ensuring the correct response is mounted against different pathogens.
This document discusses cancer immunology and immunotherapy. It begins by introducing cancer nomenclature and hallmarks. It describes how the immune system normally responds to cancer cells through immune surveillance and tumor antigen recognition. However, tumors can evolve mechanisms to evade the immune system through cancer immunoediting, where the immune response shapes tumors over time to select for less immunogenic variants. Immunotherapy aims to overcome tumor immune evasion and enhance anti-tumor immune responses.
The document summarizes key principles of the adaptive immune response. It describes how adaptive immunity arose due to limitations of innate immunity, including lack of specificity and memory. The adaptive response involves T and B lymphocytes that recognize specific antigens through cell surface receptors. Activation of T and B cells requires recognition of antigen along with costimulatory signals. This leads to clonal expansion and generation of effector cells and immunological memory. Adaptive immunity allows for tailored, amplified responses with self/non-self discrimination and immunological memory.
This lecture discusses how the immune system responds to tumors and how tumors evade the immune system. It covers various types of tumor antigens recognized by the immune system, including products of mutated genes, overexpressed proteins, and oncofetal antigens. The immune system mounts cellular and humoral responses against tumors through cytotoxic T cells, NK cells, macrophages, and antibodies. However, tumors have developed mechanisms to evade the immune system, such as antigen loss, lack of costimulation, immunosuppression, and inducing T cell apoptosis. Understanding the immune response and evasion is crucial for developing immunotherapies against cancer.
This document discusses hypersensitivity and allergy reactions. It describes the four types of hypersensitivity reactions, with a focus on Type III hypersensitivity reactions (immune complex-mediated). Type III reactions occur when large amounts of antigens form immune complexes in the bloodstream that can't be cleared by phagocytosis, causing tissue damage. Examples where this can happen include serum sickness from intravenous drug administration. The document also discusses systemic lupus erythematosus as an autoimmune disease that can involve Type III hypersensitivity reactions.
Immune complex-mediated hypersensitivity occurs when an antigen binds to an antibody within circulation, forming immune complexes that deposit in tissues. This causes inflammation and tissue damage. There are two types of antigens: exogenous like bacteria/viruses, and endogenous like self-components. When complexes activate complement or attract neutrophils, they cause inflammation and injury. Diseases include systemic lupus erythematosus, poststreptococcal glomerulonephritis, and the Arthus reaction, a localized skin necrosis from acute immune complex vasculitis.
The document discusses the organs of the immune system. It describes primary lymphoid organs like the bone marrow and thymus, which produce immune cells. Secondary lymphoid organs include lymph nodes, spleen, tonsils, and mucosa-associated lymphoid tissue (MALT). These secondary organs help activate and proliferate lymphocytes. The bone marrow produces B cells and the thymus selects T cells. Lymph nodes contain germinal centers that proliferate B cells upon antigen exposure. The spleen filters blood and mounts immune responses. MALT initiates immune responses along mucosal surfaces.
Immunology is the study of the immune system and its functions. The immune system protects the body from infection through innate and adaptive immunity. Innate immunity provides immediate defense against pathogens while adaptive immunity involves immune cells that develop memory to mount stronger responses against specific pathogens. When not functioning properly, the immune system can lead to autoimmune diseases, allergies, and cancer.
Immunodeficiency disorders are associated with defects or impairments in immune function that can be congenital or acquired. Primary immunodeficiency diseases involve genetic defects affecting B cell, T cell, or phagocytic cell development. Common symptoms include recurrent infections, failure to thrive, and increased susceptibility to opportunistic infections. HIV/AIDS is an acquired immunodeficiency disorder that progressively weakens the immune system by attacking CD4 cells, leaving the body vulnerable to opportunistic infections.
The organism possesses powerful mechanism to avoid immune auto aggression, The acquired ability of the immune system to avoid responsiveness to self antigens is defined as ‘ tolerance’ It is obtained by the cooperative efforts of central and peripheral mechanisms, which allow a rapid and efficient removal of pathogens ( Virus and Bacteria ) in the absence of self-recognition, It is a dysfunction of the immune system. The immune system protects you from disease and infection. Sometimes, though, the immune system can produce autoantibodies that attack healthy cells, tissues, and organs. This can lead to autoimmune disease.Autoimmune diseases can affect any part of the body
1) The document discusses the lymphatic and immune systems, including lymphoid organs, lymphocytes, antigen presentation, and the major histocompatibility complex.
2) It describes T lymphocyte development and function, including the roles of CD4+ and CD8+ T cells.
3) The roles of B lymphocytes and antibody production are summarized.
The document provides an overview of the immune system and autoimmune disorders. It discusses the organs and cells involved in immunity, including T cells, B cells, macrophages, and neutrophils. It describes innate and acquired immunity. Types of autoimmune disorders mentioned include rheumatoid arthritis and systemic lupus erythematosus. Key symptoms, diagnostic tests, and medical management are outlined for these conditions. Nursing interventions focus on promoting comfort, self-care, education, and adapting to life with a chronic condition.
The document discusses autoimmunity and immunodeficiency. It defines autoimmunity as the body's immune system attacking its own antigens, potentially causing tissue damage. Normally, immune tolerance prevents this. When tolerance is breached, various autoimmune diseases can occur. The document also defines and classifies primary and secondary immunodeficiencies, providing examples of defects and common infections associated with different types of immunodeficiencies. Laboratory tests for diagnosing autoimmune diseases and immunodeficiencies are also outlined.
This document discusses autoimmunity and autoimmune diseases. It begins by defining autoimmunity as a breakdown of self-tolerance mechanisms that leads to an adaptive immune response against self-antigens. This can result in chronic inflammation and autoimmune disease. Several proposed mechanisms for how autoimmunity occurs are described, including defects in central and peripheral tolerance. Factors like genetics, hormones, infections, and environmental exposures are thought to contribute to loss of self-tolerance. The document then classifies and describes some examples of organ-specific and systemic autoimmune diseases in more detail.
Hypersensitivity type 3: antibody antigen complexSara Hassan
Hypersensitivity type 3 involves antigen binding to IgG to form immune complexes that circulate in the blood and activate complement. These moderate complexes deposit in basement membranes and cause inflammation by attracting leukocytes, which can lead to anaphylactic shock. Immune complexes trigger inflammatory processes by activating complement anaphylatoxins and stimulating histamine release from basophils and mast cells. Neutrophils are also attracted to sites of immune complex deposition and release enzymes that damage tissues. Treatment involves steroids, interferons, or cyclosporin to reduce inflammation.
Autoimmune DIseases : Types, Mechanism, Diagnosis, TreatmentDr Mehul Dave
This is a presentation useful to learners of immunology as well as acadeicians. Useful in undergraduate as well as postgraduate courses. NEET students/Teachers can also get advantage of it.
This document discusses cancer immunotherapy. It begins by describing tumor antigens that can be recognized by the immune system, such as tumor-specific antigens, tumor-associated antigens, and antigens from oncogenic viruses. It then discusses how tumors evade the immune system and various approaches to immunotherapy, including active immunotherapies using vaccines made of tumor cells, purified antigens, or antigen-loaded dendritic cells. Passive immunotherapies discussed include adoptive cellular therapy, monoclonal antibodies, and immunotoxins. Clinical trials and effectiveness of different immunotherapies are also summarized.
The immune system consists of cells, proteins, and lymphoid organs that work together to protect the body from infection. The immune system has two branches: innate immunity provides a general and immediate response, while adaptive immunity provides a tailored response after initial exposure. Innate immunity involves physical barriers and cells like macrophages that recognize pathogens. Adaptive immunity involves B and T cells that recognize specific pathogens and mount stronger responses upon reexposure. Cytokines are proteins that regulate immune cell growth and activation and mediate inflammatory responses.
Introduction to hypersensitive reactionsDeepika Rana
This document provides an overview of hypersensitive reactions and their classification. It discusses the five main types of hypersensitivity reactions: type I-IV reactions which are antibody-mediated and immediate (types I-III) or delayed (type IV); and type V which involves antibody stimulation of cell surface receptors. Type I is allergy mediated by IgE antibodies. Type II involves IgG/IgM binding to cell surfaces. Type III occurs via immune complex deposition. Type IV is cell-mediated via T cells. Examples of each type are given.
Autoimmunity is caused by the immune system attacking the body's own tissues and organs. Around 5-7% of adults are affected by autoimmune diseases, which are more common in women. Left-handed individuals have a higher risk of autoimmunity. Mechanisms that can trigger autoimmunity include molecular mimicry between foreign and self-antigens, genetic factors that influence antigen presentation, and infections that dysregulate the immune response. Treatment options aim to suppress the immune system or remove autoantibodies.
The document summarizes key concepts in cancer immunology. It defines cancer and how the immune system views cancer cells. It describes how the innate and adaptive immune systems recognize and attack tumors through mechanisms like complement activation, NK cell activation, and T cell responses. It also discusses how cancers can evade the immune system through antigen loss, immunosuppressive microenvironments, and immune checkpoint proteins. The document concludes by outlining several immunotherapy approaches like non-specific immune stimulation, T cell therapy, immune checkpoint inhibitors, cancer vaccines, and monoclonal antibodies.
The major histocompatibility complex (MHC) is a set of surface proteins that present antigens to T cells. MHC molecules are classified into two groups: Class I MHC molecules are expressed on all nucleated cells and present intracellular antigens to CD8+ T cells. Class II MHC molecules are mainly expressed on antigen-presenting cells and present extracellular antigens to CD4+ T cells. MHC molecules play an important role in organ transplantation by determining tissue compatibility and in the immune response by ensuring the correct response is mounted against different pathogens.
This document discusses cancer immunology and immunotherapy. It begins by introducing cancer nomenclature and hallmarks. It describes how the immune system normally responds to cancer cells through immune surveillance and tumor antigen recognition. However, tumors can evolve mechanisms to evade the immune system through cancer immunoediting, where the immune response shapes tumors over time to select for less immunogenic variants. Immunotherapy aims to overcome tumor immune evasion and enhance anti-tumor immune responses.
The document summarizes key principles of the adaptive immune response. It describes how adaptive immunity arose due to limitations of innate immunity, including lack of specificity and memory. The adaptive response involves T and B lymphocytes that recognize specific antigens through cell surface receptors. Activation of T and B cells requires recognition of antigen along with costimulatory signals. This leads to clonal expansion and generation of effector cells and immunological memory. Adaptive immunity allows for tailored, amplified responses with self/non-self discrimination and immunological memory.
This lecture discusses how the immune system responds to tumors and how tumors evade the immune system. It covers various types of tumor antigens recognized by the immune system, including products of mutated genes, overexpressed proteins, and oncofetal antigens. The immune system mounts cellular and humoral responses against tumors through cytotoxic T cells, NK cells, macrophages, and antibodies. However, tumors have developed mechanisms to evade the immune system, such as antigen loss, lack of costimulation, immunosuppression, and inducing T cell apoptosis. Understanding the immune response and evasion is crucial for developing immunotherapies against cancer.
This document discusses hypersensitivity and allergy reactions. It describes the four types of hypersensitivity reactions, with a focus on Type III hypersensitivity reactions (immune complex-mediated). Type III reactions occur when large amounts of antigens form immune complexes in the bloodstream that can't be cleared by phagocytosis, causing tissue damage. Examples where this can happen include serum sickness from intravenous drug administration. The document also discusses systemic lupus erythematosus as an autoimmune disease that can involve Type III hypersensitivity reactions.
Immune complex-mediated hypersensitivity occurs when an antigen binds to an antibody within circulation, forming immune complexes that deposit in tissues. This causes inflammation and tissue damage. There are two types of antigens: exogenous like bacteria/viruses, and endogenous like self-components. When complexes activate complement or attract neutrophils, they cause inflammation and injury. Diseases include systemic lupus erythematosus, poststreptococcal glomerulonephritis, and the Arthus reaction, a localized skin necrosis from acute immune complex vasculitis.
The document discusses the organs of the immune system. It describes primary lymphoid organs like the bone marrow and thymus, which produce immune cells. Secondary lymphoid organs include lymph nodes, spleen, tonsils, and mucosa-associated lymphoid tissue (MALT). These secondary organs help activate and proliferate lymphocytes. The bone marrow produces B cells and the thymus selects T cells. Lymph nodes contain germinal centers that proliferate B cells upon antigen exposure. The spleen filters blood and mounts immune responses. MALT initiates immune responses along mucosal surfaces.
Immunology is the study of the immune system and its functions. The immune system protects the body from infection through innate and adaptive immunity. Innate immunity provides immediate defense against pathogens while adaptive immunity involves immune cells that develop memory to mount stronger responses against specific pathogens. When not functioning properly, the immune system can lead to autoimmune diseases, allergies, and cancer.
Immunodeficiency disorders are associated with defects or impairments in immune function that can be congenital or acquired. Primary immunodeficiency diseases involve genetic defects affecting B cell, T cell, or phagocytic cell development. Common symptoms include recurrent infections, failure to thrive, and increased susceptibility to opportunistic infections. HIV/AIDS is an acquired immunodeficiency disorder that progressively weakens the immune system by attacking CD4 cells, leaving the body vulnerable to opportunistic infections.
The organism possesses powerful mechanism to avoid immune auto aggression, The acquired ability of the immune system to avoid responsiveness to self antigens is defined as ‘ tolerance’ It is obtained by the cooperative efforts of central and peripheral mechanisms, which allow a rapid and efficient removal of pathogens ( Virus and Bacteria ) in the absence of self-recognition, It is a dysfunction of the immune system. The immune system protects you from disease and infection. Sometimes, though, the immune system can produce autoantibodies that attack healthy cells, tissues, and organs. This can lead to autoimmune disease.Autoimmune diseases can affect any part of the body
1) The document discusses the lymphatic and immune systems, including lymphoid organs, lymphocytes, antigen presentation, and the major histocompatibility complex.
2) It describes T lymphocyte development and function, including the roles of CD4+ and CD8+ T cells.
3) The roles of B lymphocytes and antibody production are summarized.
The document provides an overview of the immune system and autoimmune disorders. It discusses the organs and cells involved in immunity, including T cells, B cells, macrophages, and neutrophils. It describes innate and acquired immunity. Types of autoimmune disorders mentioned include rheumatoid arthritis and systemic lupus erythematosus. Key symptoms, diagnostic tests, and medical management are outlined for these conditions. Nursing interventions focus on promoting comfort, self-care, education, and adapting to life with a chronic condition.
The document discusses autoimmunity and immunodeficiency. It defines autoimmunity as the body's immune system attacking its own antigens, potentially causing tissue damage. Normally, immune tolerance prevents this. When tolerance is breached, various autoimmune diseases can occur. The document also defines and classifies primary and secondary immunodeficiencies, providing examples of defects and common infections associated with different types of immunodeficiencies. Laboratory tests for diagnosing autoimmune diseases and immunodeficiencies are also outlined.
This document discusses autoimmune disorders, which occur when the immune system attacks the body's own cells and tissues. It provides criteria for classifying autoimmune disorders and discusses some of the potential causes, including genetic, environmental, and hormonal factors. It also summarizes some specific autoimmune diseases like rheumatoid arthritis, systemic lupus erythematosus (lupus), and provides an overview of common treatment approaches which aim to suppress the immune system and reduce inflammation.
The document discusses various aspects of tumor immunity and tumor antigens:
1. Tumor antigens are antigens produced by tumor cells that trigger an immune response. They can be used as tumor markers for diagnosis and potentially for cancer therapy.
2. There are two main types of tumor antigens - tumor associated antigens, which increase with tumor growth, and tumor specific transplantation antigens, which develop during tumor development and prevent tumor transplantation between identical hosts.
3. The immune system typically mounts responses against tumor antigens via antibodies and cytotoxic T cells. Understanding tumor antigens is important for cancer immunology and developing immunotherapies to treat cancer.
The document discusses the molecular mechanisms of autoimmunity, including molecular mimicry, superantigens, epitope spreading, inappropriate MHC expression, polyclonal B cell activation by viruses and bacteria, and cytokine dysregulation. It also covers genes associated with autoimmunity, environmental factors like drugs and toxins, and sex differences in autoimmune diseases.
Your body's immune system protects you from disease and infection. But if you have an autoimmune disease, your immune system attacks healthy cells in your body by mistake. Autoimmune diseases can affect many parts of the body.
No one is sure what causes autoimmune diseases. They do tend to run in families. Women - particularly African-American, Hispanic-American, and Native-American women - have a higher risk for some autoimmune diseases.
There are more than 80 types of autoimmune diseases, and some have similar symptoms. This makes it hard for your health care provider to know if you really have one of these diseases, and if so, which one. Getting a diagnosis can be frustrating and stressful. Often, the first symptoms are fatigue, muscle aches and a low fever. The classic sign of an autoimmune disease is inflammation, which can cause redness, heat, pain and swelling.
The diseases may also have flare-ups, when they get worse, and remissions, when symptoms get better or disappear. Treatment depends on the disease, but in most cases one important goal is to reduce inflammation. Sometimes doctors prescribe corticosteroids or other drugs that reduce your immune response.
This document discusses autoimmune diseases, including their causes, symptoms, and treatments. Some key points:
- Autoimmune diseases occur when the immune system attacks the body's own organs and tissues, causing damage. They can affect specific organs or multiple systems.
- Common autoimmune diseases include Hashimoto's thyroiditis, type 1 diabetes, Graves' disease, rheumatoid arthritis, and systemic lupus erythematosus.
- Current treatments aim to suppress the immune system response using immunosuppressive drugs, cytotoxic drugs, plasmapheresis, or organ removal. Research is also investigating ways to induce tolerance to self-antigens or remove self-reactive immune cells.
Autoimmunity results from a failure of self-tolerance mechanisms in the immune system. This allows autoreactive lymphocytes and autoantibodies that target self-antigens to develop. A combination of genetic, environmental, and immunological factors contribute to autoimmune diseases. Tissue damage in these diseases can occur through various mechanisms, including cytotoxic antibodies, immune complexes, and T-cell mediated responses, leading to pathological changes in organs and tissues. Common autoimmune diseases involve autoreactivity against receptors, organ-specific cells, or cellular molecules like DNA and rheumatoid factor.
This document provides an overview of autoimmunity. It defines autoimmunity as the immune system attacking the body's own tissues, discusses the history of the field and how tolerance normally prevents this, and lists factors that can disrupt tolerance and lead to autoimmune disease. Some key autoimmune diseases are then described, along with criteria for diagnosing autoimmune conditions.
8 2 bio265 microbiology and immunology_2 instructor dr di bonaventuraShabab Ali
Adaptive immunity involves B cells and T cells that recognize specific pathogens. B cells produce antibodies during humoral immunity against extracellular pathogens. T cells help cellular immunity against intracellular pathogens by recognizing antigens presented on MHC proteins. Memory B and T cells develop during an adaptive immune response and provide faster protection upon reexposure to the same pathogen.
The document provides an introduction to the immune system, discussing both innate and adaptive immunity. It notes that innate immunity provides the first line of defense through physical barriers, complement proteins, and phagocytes. Adaptive immunity involves antigen-specific responses from T and B lymphocytes that result in memory, allowing for faster and stronger secondary responses - the principle behind vaccination.
Autoimmunity occurs when the immune system loses tolerance to its own tissues and mounts an immune response against them. There are several potential mechanisms for this loss of tolerance, including molecular mimicry between foreign and self antigens, sequestered self antigens being exposed to the immune system, and failures of regulatory mechanisms that normally suppress autoreactive immune cells. Autoimmune diseases result when this autoreactivity causes tissue damage. Examples include diseases caused by autoantibodies like rheumatoid arthritis, Graves' disease, and Hashimoto's thyroiditis, as well as those caused by autoreactive T cells like multiple sclerosis and insulin-dependent diabetes.
Tolerance & autoimmunity and organ specific autoimmune diseasesdr.Ihsan alsaimary
Dr. ihsan edan abdulkareem alsaimary
PROFESSOR IN MEDICAL MICROBIOLOGY AND MOLECULAR IMMUNOLOGY
ihsanalsaimary@gmail.com
mobile : 009647801410838
university of basrah - college of medicine - basrah -IRAQ
Autoimmune diseases occur when the immune system mistakenly attacks and damages healthy body tissues. There are over 80 types of autoimmune diseases, including rheumatoid arthritis, multiple sclerosis, and systemic lupus erythematosus. The causes are not fully understood but may involve genetic and environmental factors. Common treatments aim to suppress the immune system to reduce symptoms, though diagnosis and treatment can be challenging given the wide variation in symptoms and tissues affected across different autoimmune diseases.
The document provides an overview of the immune system, including:
1. It differentiates between innate and adaptive immunity and describes cells involved in each.
2. It outlines the properties of adaptive immunity including specificity and memory.
3. It describes the tissues and organs of the immune system including peripheral lymphoid organs and lymphocyte circulation.
Autoimmunity occurs when the immune system fails to distinguish self from non-self and attacks the body's own tissues. Immune tolerance is the body's normal ability to recognize self-antigens and not mount an immune response against itself. Breakdown of tolerance can occur through immunological factors like polyclonal T cell activation, genetic factors like certain HLA antigens, and microbial infections which may trigger an autoimmune response. Autoimmune diseases are either organ-specific when autoantibodies attack a single organ, or systemic when autoantibodies affect multiple tissues throughout the body. Common autoimmune diseases include Hashimoto's thyroiditis, Graves' disease, type 1 diabetes, rheumatoid arthritis, and systemic lupus erythe
2021 laboratory diagnosis of infectious diseases dr.ihsan alsaimarydr.Ihsan alsaimary
2021 laboratory diagnosis of infectious diseases
dr. ihsan alsaimary
university of basrah - college of medicine- DEPARTMENT OF MICROBIOLOGY
POBOX 696 ASHAR
BASRAH 42001
IRAQ
Advanced tumor immunology prof dr.ihsan edan alsaimary university of basrah...dr.Ihsan alsaimary
This document discusses various topics related to tumors and cancer, including:
1. The properties of cancer cells such as being clonally derived, having altered growth patterns, tissue affinities, and chromosomal abnormalities.
2. The development of tumor antigens, including tumor specific antigens from mutations and viral proteins, as well as tumor associated antigens like oncofetal antigens.
3. The process of metastasis, where tumor cells spread via direct seeding, lymphatics, or hematogenous routes, requiring changes in adhesion molecules, proteolytic enzymes and growth factors.
4. Common tumor markers used to screen and monitor cancers, including CEA, CA-125, PSA, and AFP
This document discusses inflammation. It begins by defining inflammation as the body's response to injury or infection where it aims to eliminate the cause and initiate repair. It then discusses the different causes of inflammation including physical, chemical, biological, and immunological factors. The key events of acute inflammation are also summarized, including vascular changes that cause increased blood flow and permeability, cellular events like leukocyte recruitment and phagocytosis, and the typical signs of inflammation. Chronic inflammation is described as a prolonged response where tissue injury and healing occur simultaneously.
The document summarizes the structure and immune functions of normal skin. It describes the two main layers of skin - the epidermis and dermis. The epidermis contains stratified squamous epithelium consisting of multiple layers, while the dermis contains blood vessels, nerves, hair follicles, and sebaceous and sweat glands. It also discusses the roles of Langerhans cells and dermal dendritic cells in cutaneous immunity as antigen presenting cells. The innate immune functions of skin include physical barriers provided by the epidermis, antimicrobial peptides and lipids secreted by keratinocytes, and proinflammatory cytokines like IL-1 and TNF-α that are involved in the acute inflammatory response.
Epidemiology of infectious diseases dr.ihsan alsaimarydr.Ihsan alsaimary
This document discusses infectious diseases and epidemiology. It defines key terms like infectious disease, communicable disease, and epidemiology. It describes the epidemiologic triad of agent, host, and environment and how they influence disease transmission. It also discusses different types of diseases based on occurrence (endemic, epidemic, pandemic) and transmission (direct, indirect). The causes, modes of transmission, and time periods associated with infectious diseases are explained.
Src jbbr-21-125 Dr. ihsan edan abdulkareem alsaimary PROFESSOR IN MEDICAL M...dr.Ihsan alsaimary
Dr. ihsan edan abdulkareem alsaimary
PROFESSOR IN MEDICAL MICROBIOLOGY AND MOLECULAR IMMUNOLOGY
ihsanalsaimary@gmail.com
mobile : 009647801410838
university of basrah - college of medicine - basrah -IRAQ
Src jbbr-20-120 Dr. ihsan edan abdulkareem alsaimary PROFESSOR IN MEDICAL M...dr.Ihsan alsaimary
Dr. ihsan edan abdulkareem alsaimary
PROFESSOR IN MEDICAL MICROBIOLOGY AND MOLECULAR IMMUNOLOGY
ihsanalsaimary@gmail.com
mobile : 009647801410838
university of basrah - college of medicine - basrah -IRAQ
Doi10.18535ijmsciv7i11.06 Dr. ihsan edan abdulkareem alsaimary PROFESSOR I...dr.Ihsan alsaimary
Dr. ihsan edan abdulkareem alsaimary
PROFESSOR IN MEDICAL MICROBIOLOGY AND MOLECULAR IMMUNOLOGY
ihsanalsaimary@gmail.com
mobile : 009647801410838
university of basrah - college of medicine - basrah -IRAQ
Assessment of immunomolecular_expression_and_prognostic_role_of_tlr7_among_pa...dr.Ihsan alsaimary
This document summarizes a research study that assessed the immunomolecular expression and prognostic role of Toll-like receptor 7 (TLR7) in patients with prostatitis. The study included 135 confirmed prostatitis patients and 50 control patients. DNA was extracted from blood samples and amplified using PCR to assess TLR7 expression. Results showed the PCR product for TLR7 was 149bp, indicating a high percentage of TLR7 presence. The study suggests TLR alleles may be associated with risk of prostatitis.
Dr. ihsan edan abdulkareem alsaimary
PROFESSOR IN MEDICAL MICROBIOLOGY AND MOLECULAR IMMUNOLOGY
ihsanalsaimary@gmail.com
mobile : 009647801410838
university of basrah - college of medicine - basrah -IRAQ
Estimation of Dr. ihsan edan abdulkareem alsaimary PROFESSOR IN MEDICAL MICR...dr.Ihsan alsaimary
Dr. ihsan edan abdulkareem alsaimary
PROFESSOR IN MEDICAL MICROBIOLOGY AND MOLECULAR IMMUNOLOGY
ihsanalsaimary@gmail.com
mobile : 009647801410838
university of basrah - college of medicine - basrah -IRAQ
Dr. ihsan edan abdulkareem alsaimary
PROFESSOR IN MEDICAL MICROBIOLOGY AND MOLECULAR IMMUNOLOGY
ihsanalsaimary@gmail.com
mobile : 009647801410838
university of basrah - college of medicine - basrah -IRAQ
Dr. ihsan edan abdulkareem alsaimary
PROFESSOR IN MEDICAL MICROBIOLOGY AND MOLECULAR IMMUNOLOGY
ihsanalsaimary@gmail.com
mobile : 009647801410838
university of basrah - college of medicine - basrah -IRAQ
Dr. ihsan edan abdulkareem alsaimary
PROFESSOR IN MEDICAL MICROBIOLOGY AND MOLECULAR IMMUNOLOGY
ihsanalsaimary@gmail.com
mobile : 009647801410838
university of basrah - college of medicine - basrah -IRAQ
Dr. ihsan edan abdulkareem alsaimary
PROFESSOR IN MEDICAL MICROBIOLOGY AND MOLECULAR IMMUNOLOGY
ihsanalsaimary@gmail.com
mobile : 009647801410838
university of basrah - college of medicine - basrah -IRAQ
The document discusses laboratory diagnosis of infectious diseases. It covers proper selection, collection and transport of clinical specimens; laboratory tests including microscopy, culture techniques, and biochemical reactions; and specimens from different anatomical sites. Key points are the importance of collecting specimens before antimicrobial therapy, avoiding contamination, and transporting specimens in a way that preserves viable organisms. A proper diagnosis requires integrating clinical information with laboratory findings.
Pathogenesis of microbial infections dr. ihsan alsaimarydr.Ihsan alsaimary
Dr. ihsan edan abdulkareem alsaimary
PROFESSOR IN MEDICAL MICROBIOLOGY AND MOLECULAR IMMUNOLOGY
ihsanalsaimary@gmail.com
mobile : 009647801410838
university of basrah - college of medicine - basrah -IRAQ
Dr. ihsan edan abdulkareem alsaimary
PROFESSOR IN MEDICAL MICROBIOLOGY AND MOLECULAR IMMUNOLOGY
ihsanalsaimary@gmail.com
mobile : 009647801410838
university of basrah - college of medicine - basrah -IRAQ
Dr. ihsan edan abdulkareem alsaimary
PROFESSOR IN MEDICAL MICROBIOLOGY AND MOLECULAR IMMUNOLOGY
ihsanalsaimary@gmail.com
mobile : 009647801410838
university of basrah - college of medicine - basrah -IRAQ
Dr. ihsan edan abdulkareem alsaimary
PROFESSOR IN MEDICAL MICROBIOLOGY AND MOLECULAR IMMUNOLOGY
ihsanalsaimary@gmail.com
mobile : 009647801410838
university of basrah - college of medicine - basrah -IRAQ
Muktapishti is a traditional Ayurvedic preparation made from Shoditha Mukta (Purified Pearl), is believed to help regulate thyroid function and reduce symptoms of hyperthyroidism due to its cooling and balancing properties. Clinical evidence on its efficacy remains limited, necessitating further research to validate its therapeutic benefits.
Integrating Ayurveda into Parkinson’s Management: A Holistic ApproachAyurveda ForAll
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These lecture slides, by Dr Sidra Arshad, offer a quick overview of the physiological basis of a normal electrocardiogram.
Learning objectives:
1. Define an electrocardiogram (ECG) and electrocardiography
2. Describe how dipoles generated by the heart produce the waveforms of the ECG
3. Describe the components of a normal electrocardiogram of a typical bipolar lead (limb II)
4. Differentiate between intervals and segments
5. Enlist some common indications for obtaining an ECG
6. Describe the flow of current around the heart during the cardiac cycle
7. Discuss the placement and polarity of the leads of electrocardiograph
8. Describe the normal electrocardiograms recorded from the limb leads and explain the physiological basis of the different records that are obtained
9. Define mean electrical vector (axis) of the heart and give the normal range
10. Define the mean QRS vector
11. Describe the axes of leads (hexagonal reference system)
12. Comprehend the vectorial analysis of the normal ECG
13. Determine the mean electrical axis of the ventricular QRS and appreciate the mean axis deviation
14. Explain the concepts of current of injury, J point, and their significance
Study Resources:
1. Chapter 11, Guyton and Hall Textbook of Medical Physiology, 14th edition
2. Chapter 9, Human Physiology - From Cells to Systems, Lauralee Sherwood, 9th edition
3. Chapter 29, Ganong’s Review of Medical Physiology, 26th edition
4. Electrocardiogram, StatPearls - https://www.ncbi.nlm.nih.gov/books/NBK549803/
5. ECG in Medical Practice by ABM Abdullah, 4th edition
6. Chapter 3, Cardiology Explained, https://www.ncbi.nlm.nih.gov/books/NBK2214/
7. ECG Basics, http://www.nataliescasebook.com/tag/e-c-g-basics
- Video recording of this lecture in English language: https://youtu.be/kqbnxVAZs-0
- Video recording of this lecture in Arabic language: https://youtu.be/SINlygW1Mpc
- Link to download the book free: https://nephrotube.blogspot.com/p/nephrotube-nephrology-books.html
- Link to NephroTube website: www.NephroTube.com
- Link to NephroTube social media accounts: https://nephrotube.blogspot.com/p/join-nephrotube-on-social-media.html
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We’re talking about Vedic Meditation, a form of meditation that has been around for at least 5,000 years. Back then, the people who lived in the Indus Valley, now known as India and Pakistan, practised meditation as a fundamental part of daily life. This knowledge that has given us yoga and Ayurveda, was known as Veda, hence the name Vedic. And though there are some written records, the practice has been passed down verbally from generation to generation.
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In some case, your chronic prostatitis may be related to over-masturbation. Generally, natural medicine Diuretic and Anti-inflammatory Pill can help mee get a cure.
Osteoporosis - Definition , Evaluation and Management .pdfJim Jacob Roy
Osteoporosis is an increasing cause of morbidity among the elderly.
In this document , a brief outline of osteoporosis is given , including the risk factors of osteoporosis fractures , the indications for testing bone mineral density and the management of osteoporosis
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Overall life span (LS) was 1671.7±1721.6 days and cumulative 5YS reached 62.4%, 10 years – 50.4%, 20 years – 44.6%. 94 LCP lived more than 5 years without cancer (LS=2958.6±1723.6 days), 22 – more than 10 years (LS=5571±1841.8 days). 67 LCP died because of LC (LS=471.9±344 days). AT significantly improved 5YS (68% vs. 53.7%) (P=0.028 by log-rank test). Cox modeling displayed that 5YS of LCP significantly depended on: N0-N12, T3-4, blood cell circuit, cell ratio factors (ratio between cancer cells-CC and blood cells subpopulations), LC cell dynamics, recalcification time, heparin tolerance, prothrombin index, protein, AT, procedure type (P=0.000-0.031). Neural networks, genetic algorithm selection and bootstrap simulation revealed relationships between 5YS and N0-12 (rank=1), thrombocytes/CC (rank=2), segmented neutrophils/CC (3), eosinophils/CC (4), erythrocytes/CC (5), healthy cells/CC (6), lymphocytes/CC (7), stick neutrophils/CC (8), leucocytes/CC (9), monocytes/CC (10). Correct prediction of 5YS was 100% by neural networks computing (error=0.000; area under ROC curve=1.0).
2. AUTOIMMUNITY
Ability of immune system to differentiate
between self and non-self antigens
Immune system response against self
antigens
3. AUTOIMMUNITY
Autoimmunity appears normal part of
immune system
Healthy people have low concentration
of autoantibodies in serum and tissue
Auto-antibodies may form antigen-
antibody complex removed by
macrophages as part of tissue damage
removal
5. T Cells Mature
in Thymus
Stem Cells
of the Bone
Marrow
Identify
Antigens
B Cells Replicate
to form
Plasma cells
B Memory
Cells
Released into
blood, spleen,
lymph
Macrophages
carry foreign
cells to T
Helper cells
T Helper cells (Th)
produce proteins
Secrete
Interleukins
Secrete
lymphokines
Release
Antibodies
Stimulates
Phagocytosis
Effector Tc
Cells
Tm Memory
Cells
B Cells Mature
in Marrow
Replicate
Cytotoxic (killer)
T (Tc) Cells
Antibody Mediated
Immunity
Cell Mediated
Immunity
Lymphocyte
Maturation
7. Autoimmune Diseases
When the immune system attacks the
body's own cells, it produces an
autoimmune disease.
8. Autoimmune Diseases
Some examples of autoimmune
diseases include:
Type I diabetes attacks insulin-producing cells.
Rheumatoid arthritis attacks connective tissues
around joints.
Myasthenia gravis attacks neuromuscular
junctions.
Multiple sclerosis (MS) destroys functions of
brain and spinal cord neurons.
9. Autoimmune Diseases
Some autoimmune diseases are treated
with medications that alleviate specific
symptoms.
10. Immunological Problems & Diseases
There are several ways in which the immune system may fail:
When the pathogen is too violent (multiplies too fast, causes
too much damage), or evades the immune system (e.g., via
mutation). Solution: vaccination or medication.
Immune deficiencies: inherited or acquired.
Improper response to foreign (non-pathogenic) antigens:
Hypersensitivity and Allergy.
Improper response to self: Autoimmune diseases.
Rejection of transplanted tissues.
Failure to detect cancers.
[Cancer of immune cells.]
11. Immune Deficiencies
Inherited:
Cellular - when the defective gene is only in T cells;
Humoral - when the defective gene is only in B cells;
Combined - when the defect is in a gene common to all
lymphocytes, e.g., RAGs (recombination activation genes).
Acquired - due to:
Hemopoietic diseases;
Treatments: chemotherapy, irradiation;
Infection: AIDS - caused by the Human Immunodeficiency
Virus (HIV) which attacks helper T cells. The virus gradually
kills more T cells than the body can produce, the immune
system fails, and the patient dies from infections that are
normally not dangerous.
12. Immune Hypersensitivity
Hypersensitivity is an improperly strong response.
Immediate hypersensitivity:
Mediated by antibodies.
Types:
allergy - up to anaphylactic shock.
Induction of antibody-mediated cytotoxicity.
Sickness due to accumulation of immune complexes.
Delayed hypersensitivity:
Mediated by T cells.
Hyper-activity of CTLs and macrophages.
Contact sensitivity.
13. Allergy
Allergy is an immune response
to harmless antigens.
Mechanism: IgE bind Fce
receptors on mast cells and
basophils, and causes release
of granules with inflammatory
agents.
The “real” role of IgE is
probably to fight parasites such
as helminths. (In developing
countries, people hardly ever
suffer from allergies.)
14. Autoimmune diseases
Normally, the immune system does not attack the self.
This is ensured by elimination of auto-reactive lymphocytes
during their development (negative selection).
However, there is a large group of diseases in which the
immune system does attack self-cells: autoimmune diseases.
The attack can be either humoral (by auto-antibodies) or
cellular (by auto-reactive T cells).
The attack can be directed either against a very specific
tissue, or to a large number of tissues (systemic autoimmune
disease), depending on the self-antigen which is attacked.
15. Autoimmune diseases
Specific:
Juvenile diabetes (attacks insulin-producing cells)
Multiple sclerosis (attacks myelin coating of nerve axons)
Myasthenia gravis (attacks nerve-muscle junction)
Thyroiditis (attacks the thyroid)
…
Systemic: Immune complexes accumulate in many tissues and
cause inflammation and damage.
Systemic Lupus Erythematosus (anti-nuclear antibodies):
harms kidneys, heart, brain, lungs, skin…
Rheumatoid Arthritis (anti-IgG antibodies): joints, hearts,
lungs, nervous system…
Rheumatic fever: cross-reaction between antibodies to
streptococcus and auto-antibodies.
16. What could cause the immune
system to attack the self?
Changes in self-antigens, that make them look like non-self to
the immune system, due to:
Viral or bacterial infection
Irradiation
Medication
Smoking …
Changes in the immune system:
Normal auto-antibodies exist; mutations in B cells producing
them may create pathogenic auto-antibodies.
Problems with control of lymphocyte development and
differentiation.
17. Transplant Rejection
The T lymphocyte repertoire is selected to tolerate cells
expressing self-MHC-I + self-peptide complexes, and attack
non-self (altered) complexes.
Normally, altered complexes would be the result of infection
or transformation of the cell expressing the MHC, that is, the
peptide will be non-self.
However, transplantation of tissues from a non-MHC-matched
donor will present to the immune system a non-self-MHC
(with self-peptides, usually).
The immune system will react vigorously against this “altered
self”.
Prevention: finding a matched donor or immune suppression.
18. Failure to detect Cancer cells
Cancer is uncontrolled proliferation of self-cells. Cancer cells
have lost the mechanisms of cell cycle control, dependence
on resources or cell density, etc. Later on, some of the
tumor cells may migrate to other body sites (metastasis).
Transformation from normal to cancerous cells involves many
genetic, biochemical, metabolic changes in the cells.
The immune system sometimes recognizes these changes
and regards the transformed cells as “ altered self ” to be
attacked.
When will the immune system fight cancer:
When it’s different enough from self,
When the quantity of non-self cells is large enough,
When the system functions well, and is not suppressed.
19.
20. Development of
Autoimmune Disease
Autoimmune disease occurs as a result of breakdown
in tolerance to self
Autoimmune disease is characterized by immune
system “attack” against self antigens that lead to
tissue damage
– Inflammation and hypersensitivity reactions
Mediated by B-lymphocytes that produce antibodies
to self antigens
And / or
T-lymphocytes with T-cell receptors that recognize
selfantigens
– Autoreactive CD4 Th and autoreactive CD8
cytotoxic T cells
21. Mechanisms of tissue injury
Auto-antibodies circulating in blood or
at site of tissue injury
Autoreactive B and T cells in blood and
at site of tissue injury
Hypersensitivity reactions occurring at
sites of tissue injury
Histology- chronic inflammation
Pathway for cell death- apoptosis
22. TYPES OF HYPERSENSITIVITY
Type II
– Autoimmune hemolytic anemias
– Anti-insulin receptor antibody (insulin-
dependent diabetes mellitus )
Type III
– Systemic lupus erythematosus (SLE)
– Rheumatoid arthritis
23. SYSTEMIC LUPUS
ERYTHEMATOSUS (SLE)
Chronic systemic autoimmune disease
Cause unknown
Affects almost any organ(s)
Characterized by chronic inflammation
24. SYSTEMIC LUPUS
ERYTHEMATOSUS (SLE)
Auto-antibodies formed against variety of self
antigens
Anti-double stranded DNA,RNA and histones
Antibodies against cell surface antigens on
RBC’s and/or platelets
Tissue damage caused by Type III
hypersensitivity reactions
Immune circulating complexes formed
against self deposit on tissues
Vasculitis, synovitis, glomerulonephritis
25. Systemic lupus
erythematosis is the most
commonly known
autoimmune disorder. This
characteristic “butterfly”
rash is made worse by
exposure to sunlight.
Lupus is a potentially fatal
autoimmune disease that
strikes 1 in 2,000
Americans and 10 times as
many women as men.
26. Rheumatoid Arthritis
Systemic autoimmune disease
Genetic factors (HLA-DR1, HLA-DR4)
Autoreactive B-cells synthesize auto antibody
against Fc portion of IgG
Rheumatoid factor (RF)
Chronic inflammation of synovial joints
Proliferation of synovial lining cells
Erosion of articular cartilage and adjacent
bone
27. Rheumatoid arthritis (RA)
affects peripheral joints and
may cause destruction of
both cartilage and bone. The
disease affects mainly
individuals carrying the DR4
variant of MHC genes.
This fact can lead to better
prognoses and in aiding
efforts to change immune
reactions that involve the
DR4 variant while leaving
other reactions intact.
30. The antibody is 4 polypeptides forming
a Y-shaped structure.
Each side of the Y is composed of one
light chain and 1 heavy chain.
The 2 arms (Fab regions) contain
antigen binding sites.
The stem of the Y is the Fc region.
34. What happens when the immune system
malfunctions?
1. Antibodies are produced
35. What happens when the immune system
malfunctions?
1. Antibodies are produced
2. Stems of antibodies
attach to mast cells,
especially in the respiratory
tract
36. What happens when the immune system
malfunctions?
1. Antibodies are produced
2. Stems of antibodies
attach to mast cells,
especially in the respiratory
tract
3. When
antibodies
attached to mast
cells bind antigens, the mast
cells release histamine, which causes inflammation
37. Autoimmune diseases
The immune system lacks or loses its ability to
distinguish self vs. non-self molecules, i.e., it
loses its self-tolerance and produces anti-self
antibodies
What happens when the immune system
malfunctions?
Rheumatoid arthritis (cartilage of joints)
Multiple sclerosis (mylein sheaths of
neurons)
Insulin-dependent diabetes mellitus (insulin-
secreting cells of the pancreas)
38. What happens when the immune system
malfunctions?
Severe Combined Immunodeficiency (SCID)
An inherited disorder
Acquired Immunodeficiency Syndrome (AIDS)
Caused by retroviruses (Human
Immunodeficiency Viruses – HIV) that
especially infect helper T cells
Immunodeficiency diseases
Inhibit effective immune response; either
inherited or acquired
39.
40. Lymphocytes recognize and respond to particular
microbes and foreign molecules, i.e.,
they display specificity
A foreign molecule
that induces an
immune response
is known as an
antigen
RECOGNITION
42. B cells produce antibodies, that are either secreted
out of the cells or remain embedded in the B cell
membranes, and that bind to antigens
RECOGNITION
43. B cells produce antibodies, that are either secreted
out of the cells or remain embedded in the B cell
membranes, and that bind to antigens
T cells have T-cell receptors, embedded in their
cell membranes, that bind to antigens
RECOGNITION
44. RECOGNITION
Secreted antibodies con-
stitute a group of proteins
called immunoglobulins
Antibodies have 2
heavy chain and 2 light
chain subunits
Each subunit has a
constant region and a
variable region
The variable region can
bind to an antigen
45. Construction of antibodies
(and T-cell receptors)
Millions of antigens are recognized by randomly
combining the protein products of hundreds of genes
RECOGNITION of non-self molecules
Card analogy: although there are only 52 cards in the
deck, random combinations can produce an
enormous number of different hands
46. In a healthy immune system, as B and T cells mature
they are destroyed by apoptosis if they attack self
molecules
RECOGNITION of self molecules
Healthy, mature B and T cells then have the capacity
to distinguish self from non-self molecules
47. Almost all cells in an individual human’s body have
major histocompatibility complex (MHC)
glycoproteins embedded in their cell membranes
RECOGNITION of self molecules
Class I MHC molecules are found on
almost every nucleated cell
Class II MHC molecules are restricted to a few
specialized cells, including macrophages,
dendritic cells, B cells, etc.
48. MHC glycoproteins migrate to the cell
membrane after they are produced
RECOGNITION of self molecules
MHC glycoproteins pick up molecules from the cytosol
that are presented at the cell’s surface
T cells bind to MHC glycoproteins and
the molecules they present
An individual’s own MHC glycoproteins, and
molecules of its own body that the MHC
glycoproteins present, are treated as self
49. Helper T cells bind to cells that carry
Class II MHC glycoproteins
RECOGNITION of non-self molecules
50. ATTACK & MEMORY
The B and T cells that first recognize a given foreign
antigen are short lived, whereas immune memory
cells can have long lifetimes
Illustrated here
for B cells, but
the process for
T cells is similar
52. Introduction
Under normal circumstances immune system
will not destroy self antigens.
Autoimmunity can be defined as breakdown of
mechanisms responsible for self tolerance and
induction of an immune response against
components of the self.
In numerous autoimmune diseases it is well
recognized that products of the immune system
cause damage to the self.
54. Autoimmune Response
Antibody directed against “self”, termed auto-
antibody
Considered abnormal but usually does not result
in disease.
May occur in healthy individuals.
55. Autoimmune Disease
Disorder in which tissue injury is caused by an
immunologic reaction of the host to its own
tissues.
Precise mechanisms unknown.
Classified as systemic or organ specific,
frequently have overlap.
57. Forbidden clone
Clone of changed or altered lymphocytes arise
through mutation.
Lack foreign surface antigens, not destroyed.
Because of alteration may recognize host as
foreign.
58. Altered Antigen
Surface antigens on host altered by chemical,
biological or physical means.
This new antigenic determinant may be
recognized as foreign by the host.
59. Sequestered Antigen
Some antigens in the body are hidden from cells
of the immune system.
If there is damage to these organs causing
exposure of these sequestered antigens an
immune reaction to these antigens may occur.
60. Immunologic Deficiency Theory
Relates the increased frequency of auto-
antibodies and increased immune system
deficiency to age.
Mutation or loss of immune regulatory powers
results in the condition in which self antigens
behave as foreign antigens.
61. Genetic Influence
It is well recognized that certain immune
disorders predominate in females and in
families.
Determined by family studies.
Genetic links have occurred between diseases
and HLA antigens
63. Classification of Autoimmune
Diseases
Systemic- the auto-immunity is directed against
an antigen that is present at many different sites
and can include involvement of several organs
Organ specific - Organ specific means the auto-
immunity is directed against a component of
one particular type of organ.
Both – can get overlap
64.
65. Systemic Lupus Erythematosus
Chronic, systemic inflammatory disease caused by
immune complex formation.
The word "systemic" means the disease can affect many
parts of the body.
Pathophysiology associated with clinical features
secondary to immune complexes depositing in tissues
resulting in inflammation.
Parts of the body affected include: the joints, skin,
kidneys, heart, lungs, blood vessels, and brain.
66. Systemic Lupus Erythematosus
Peak age of onset is 20 to 40 years of age.
Found more frequently in women.
Has both genetic and environmental factors.
67. SLE Clinical Signs
Extremely diverse and nonspecific.
Joint involvement most frequent sign:
polyarthralgia and arthritis occur in 90% of
patients.
Skin manifestations next most common.
Erythematosus rash may appear.
Most classic is butterfly rash.
68. SLE Butterfly Rash
The source of the name "lupus" is
unclear. All explanations originate with
the characteristic butterfly-shaped
malar rash that the disease classically
exhibits across the nose and cheeks.
In various accounts, some doctors
thought the rash resembled a wolf
pattern. In other accounts doctors
thought that the rash, which was often
more severe in earlier centuries, created
lesions that resembled wolf bites or
scratches.
Stranger still, is the account that the
term "Lupus" didn't come from latin at
all, but from the term for a French
style of mask which women reportedly
wore to conceal the rash on their faces
69. SLE Clinical Signs
Renal involvement very common.
Caused by deposition of immune complexes in
kidney tissue.
Leads to renal failure, most common cause of death.
Other systemic effects:
Cardiac
Central nervous system.
Hematologic abnormalities.
70. Immunologic Findings
Lupus Erythematosus (LE) cell, neutrophil which has
engulfed the antibody-coated nucleus of another cell.
First classic test to aid in diagnosis.
Not utilized anymore, may still see in older references.
Over activity of B cells main immunologic
characteristic.
Antinuclear antibodies produced.
More than 28 antibodies associated with LE have been
identified.
Level of antibody production correlates with severity of
symptoms.
Estrogen enhance B cell activation.
71. LE Cell
Here is the famous "LE cell" test which has value only in
demonstrating how the concept of autoantibodies work. The
pink blobs are denatured nuclei. Here are two, with one seen
being phagocytozed in the center by a PMN. This test is not
nearly as sensitive as the ANA which has supplanted the LE cell
test. Therefore, NEVER order an LE cell test. [Image
contributed by Elizabeth Hammond, MD, University of Utah]
72. Immunologic Findings
Decrease in absolute number of T cells
Accumulation of immune complexes with
activation of complement lead to kidneydamage.
Drug induced lupus may occur, discontinue
drug, symptoms usually disappear.
73. Laboratory Diagnosis
Screening test for anti-nuclear antibodies (ANA) first
test done.
Antibodies directed against nuclear material of cells.
Flourescent anti-nuclear antibody (FANA) most widely
used, extremely sensitive, low diagnostic specificity.
Animal or human cells fixed to slide.
Add patient serum and incubate.
Wash to remove unreacted antibody.
Add anti-human globulin labeled with fluorescent
tag or enzyme.
74. ANA
Patterns of reactivity:
Homogenous-entire nucleus stained
Peripheral-rim of nucleus stained
Speckled-spots of stain throughout nucleus
Nucleolar-nucleolus only stained
False positives and negatives occur.
If positive, perform profile testing.
75. Antinuclear Antibody Test
Antinuclear antibodies
(ANA) are autoantibodies
against various cell nucleus
antigens and are found in
patients with autoimmune
diseases such as SLE.
Some of ANA are considered
to be useful for diagnosis of
autoimmune diseases.
77. Nucleolar
23 or 46 (or some multiple of 46) bright speckles or
ovoid granules spread over the nucleus of interphase
cells
78. Peripheral
Fluorescence is most intense at the periphery of the
nucleus with a large ring starting from the internal
nuclear membrane and the rest of the nucleus showing
weaker yet smooth staining.
79. Speckled
Large speckles covering the whole nucleoplasm,
interconnected by a fine fluorescent network.
80. Anti-nuclear antibodies detected by FANA
Double-stranded DNA (ds-DNA) antibodies are most specific
for SLE, correlate well with disease activity.
Antihistone antibody second major antibody found in SLE.
Deoxyribonucleoprotein (DNP) antibody, responsible for LE
cell phenomena and available as a latex agglutination test.
Anti-Sm antibody, specific for LE.
SS-A/Ro and SS-B/La antibodies, most common in patients
with cutaneous manifestations.
Anti-nRNP detected in patients with SLE as well as mixed
connective tissue disease.
Presence of antibodies not diagnostic, may be present due to
other diseases.
81. Anti-nuclear Antibodies by
Immunodiffusion.
Used to determine specificity.
Ouchterlony double diffusion most frequently
used to identify antibodies to: Sm, nRNP, SS-
A/Ro, SS-B/La and others.
Test is not as sensitive but very specific.
82. Extractable Nuclear Antigen
This is antibody to a cytoplasmic ribonuclear
protein complex.
It is associated with mixed connective disease
and SLE with particular features (arthritis,
myositis, Raynaud's phenomenon - also
association with HLA-DR4 and HLA-DQw8).
85. Antiphospholipid Antibodies
Antiphospholipid antibodies may be present and
are of two types.
Anticardiolipin.
Lupus anticoagulant, if present, may cause
spontaneous abortion and increase
Risk of clotting, platelet function may be
affected.
86. Treatment
Aspirin and anti-inflammatories for fever and
arthritis.
Skin manifestations-anti-malarials or topical
steroids.
Systemic corticosteroids for acute fulminant
lupus, lupus nephritis or central nervous system
complications.
Five year survival rate is 80 to 90%.
87. Rheumatoid Arthritis
Chronic inflammatory disease primarily affecting the
joints, but can affect heart, lung and blood vessels.
Women three more times as likely as men to have it.
Typically strikes at ages between 20 and 40, but can
occur at any age.
The three major symptoms of arthritis are joint
pain, inflammation, and stiffness.
Progress of disease varies.
88. Clinical Signs
Diagnosis based on criteria established by
American College of Rheumatologists, must
have at least 4 of the following:
Morning stiffness lasting 1 hour.
Swelling of soft tissue around 3 or more joints.
Swelling of hand/wrist joints.
Symmetric arthritis.Subcutaneous nodules
Positive test for rheumatoid factor.
Xray evidence of joint erosion.
89. Clinical Signs
Symptoms initially non-specific: malaise, fever, weight
loss, and transient joint pain.
Morning stiffness and joint pain improve during the day.
Symmetric joint pain: knees, hips, elbows, shoulders.
Joint pain leads to muscle spasm, limits range of motion,
results in deformity.
Approximately 25% of patients have nodules over
bones (necrotic areas), nodules can also be found in
organs.
Certain bacteria may trigger RA due to certain proteins
that possess antigens similar to those antigens found in
joint, ie, molecular mimicry
90. Immunologic Findings
Rheumatoid Factor (RF) is an IgM antibody
directed against the Fc portion of the IgG
molecule, it is an anti-antibody.
Not specific for RA, found in other diseases.
Immune complexes form and activate
complement and the inflammatory response.
Enzymatic destruction of cartilage is followed by
abnormal growth of synovial cells, results in the
formation of a pannus layer.
92. Diagnosis
Diagnosis is based on:
Clinical findings.
Radiographic findings
Laboratory testing.
Laboratory tests involve testing patients serum with red blood
cells or latex particles coated with IgG, agglutination is a positive
result.
Nephelometry and ELISA techniques are available to quantitate
the RF.
Erythrocyte Sedimentation Rate (ESR) used to monitor
inflammation.
C-Reactive protein (CRP) is utilized to monitor inflammation
93. Treatment
Rest and nonsteroidal anti-inflammatory drugs
control swelling and pain.
Substantial functional loss seen in 50% of
patients within 5 years.
Slow acting antirheumatic drugs are coming into
use but have side affects.
Joint replacement.
94. Hashimoto's Thyroiditis
Hashimoto's Thyroiditis is a type of autoimmune
thyroid disease in which the immune system attacks and
destroys the thyroid gland.
The thyroid helps set the rate of metabolism - the rate
at which the body uses energy.
Hashimoto’s prevents the gland from producing
enough thyroid hormones for the body to work
correctly.
It is the most common form of Hypothyroidism
(underactive thyroid).
95. Hashimoto’s Thyroiditis
Organ specific disease affecting the thyroid
gland.
Most often seen in women 30 to 40 years old,
may be genetic predisposition.
Common cause of hypothyroidism.
Causes diffuse hyperplasia in the gland resulting
in development of a goiter.
Thyroid autoantibodies are formed.
96. Hashimoto’s Thyroiditis
Hashimoto's thyroiditis is the most common
cause of hypothyroidism.
It is also most prevalent in elderly women and
tends to run in families.
Hashimoto's thyroiditis occurs eight times more
often in women than men.
Certain chromosomal abnormalities include
Hashimoto's thyroiditis as a symptom.
97. Symptoms
The following are the most common symptoms.
However, each individual may experience symptoms
differently:
goiter (enlarged thyroid gland which may cause a bulge in the
neck)
other endocrine disorders such as diabetes, an underactive
adrenal gland, underactive parathyroid glands, and other
autoimmune disorders
fatigue
muscle weakness
weight gain
98. Thyroid
Thyroid hormones are produced by the thyroid gland. This gland
is located in the lower part of the neck, below the Adam's apple.
The gland wraps around the windpipe (trachea) and has a shape
that is similar to a butterfly - formed by two wings (lobes) and
attached by a middle part (isthmus).
99. Goiter
This enlargement is due to the inflammatory cells which destroy
thyroid cells, resulting in long term scarring. When the cells are
damaged they cease thyroid hormone production, resulting in
hypothyroidism
A goiter only needs to be treated if it is causing symptoms.
The enlarged thyroid can be treated with radioactive iodine to
shrink the gland or with surgical removal of part or all of the
gland (thyroidectomy).
Small doses of iodine (Lugol's or potassium iodine solution) may
help when the goiter is due to iodine deficiency.
100. Laboratory Testing
The diagnosis of Hashimoto's thyroiditis is simply diagnosed by
two blood tests.
Routine thyroid function tests to confirm that a patient has an
underactive thyroid gland.
Anti-microsomal and anti-thyroglobulin antibodies are immune
cells which the body produces to attack specific portions of the
thyroid cells which pinpoint Hashimoto's thyroiditis as the cause
of the hypothyroidism.
The anti-microsomal antibody test is much more sensitive than
the anti-thyroglobulin, therefore some doctors use only the
former blood test.
These thyroid autoantibodies blood tests are high in about 95%
of patients with Hashimoto's thyroiditis, but are not diagnostic.
102. Graves’ Disease - Thyrotoxicosis
Characterized by HYPERTHYROIDISM.
Nervousness, insomnia, depression, weight loss,
heat intolerance, breathlessness, fatigue, cardiac
dysrhythmias, and restlessness.
Women more susceptible, occurs most
frequently between 30 and 40 years of age.
Genetic link suspected.
103. Graves’ Disease
Diagnosis may be straightforward, since the "classic
face" with its triad of hyperthyroidism, goiter, and
exophthalmos is easily recognized.
Goiter is usually symmetric, smooth, and nontender
The hyperthyroid state, which is by far the most
common component of Graves' disease, can cause a
wide variety of multisystem derangements that often
result in diagnostic confusion.
104. Exophthalmos
Exophthalmos, also called proptosis, is a characteristic
finding in thyroid eye disease, and has been reported
to occur in 34% to 93% of patients
105. Signs Symptoms
Nervousness and increased activity, Grave's
disease patients may suffer a fast heartbeat,
fatigue, moist skin, increased sensitivity to heat,
shakiness, anxiety, increased appetite, weight
loss, and sleep difficulties.
They also have at least one of the following: an
enlargement of the thyroid gland (goiter),
bulging eyes, or raised areas of skin over the
shins.
106. Laboratory Testing
Presence of thyroid-stimulating hormone
receptor antibody, causes release of thyroid
hormones.
Key findings are elevated total and free T3
(triiodothyronine) and T4 (thyroxine), the
thyroid hormones.
Thyroid stimulating hormone (TSH) is reduced
due to antibody stimulation of the thyroid.
108. Insulin Dependent Diabetes Mellitus
Autoimmune process causes destruction of cells
in the pancreas resulting in insufficient insulin
production.
Occurs before age 20, peak onset between 10
and 14 years.
Inherited susceptibility.
Environmental influences include possibility of
viral infections.
109. Complications
With its complications, diabetes is the seventh leading
cause of death in the United States.
Diabetes is the leading cause of new blindness in people
20-74 years of age.
Ten to twenty-one percent of all people with diabetes
develop kidney disease.
People with diabetes are 2-4 times more likely to have
heart disease.
About 60%-70% of people with diabetes have mild to
severe forms of diabetic nerve damage, which, in severe
forms, can lead to lower limb amputations.
110. Laboratory Testing
The American Diabetes Association (ADA) recommendations
for diagnosing diabetes state that patients be told they have
diabetes if any of the criteria below applies:
Fasting plasma glucose is above 126 mg/dl;
Diabetes symptoms exist and casual plasma glucose is equal to or above
200 mg/dl; or
Plasma glucose is equal to or above 200 mg/dl during an oral glucose
tolerance test.
The ADA now also recommends that all individuals age 45 and
above be tested for diabetes, and if the test is normal, they
should be re-tested every three years.
If genetic predisposition is suspected perform testing to detect
antibodies to pancreatic islet cells.
Antibodies to insulin detected by RIA or ELISA methods.
111. Indications for Laboratory Testing
Testing should be conducted at earlier ages and carried out more
frequently in individuals who are any of the following:
obese;
have a first degree relative with diabetes;
are members of a high-risk ethnic population (African-American,
Hispanic, Native American, Asian);
have delivered a baby weighing more than 9 pounds;
have had gestational diabetes;
are hypertensive;
have HDL cholesterol levels equal to or less than 35 mg/dl or triglyceride
levels equal to or greater than 250 mg/dl;
or who, on previous testing had impaired glucose tolerance or impaired
fasting glucose.
113. Multiple Sclerosis
Multiple sclerosis (MS) is a chronic, potentially debilitating
disease that affects the brain and spinal cord (central nervous
system).
Destruction of myelin sheath of axons results in formation of
lesions (plaques) in white matter of brain and spinal cord.
Causes inflammation and injury to the sheath and ultimately to
the nerves.
The result may be multiple areas of scarring (sclerosis).
Cause may include genetic and environmental factors.
Most often seen between ages of 20 and 50.
114. Multiple Sclerosis
Because the myelin is damaged, messages moving along
the nerve are transmitted more slowly or not at all
which slows or blocks muscle coordination, visual
sensation and other nerve signals.
116. Diagnosis
The basic guideline for diagnosing MS relies on two
criteria:
There must have been two attacks at least one month apart.
An attack, also known as an exacerbation, flare, or relapse, is
a sudden appearance of or worsening of an MS symptom or
symptoms which lasts at least 24 hours.
There must be more than one area of damage to central
nervous system myelin—the sheath that surrounds and
protects nerve fibers. The damage to myelin must have
occurred at more than one point in time and not have been
caused by any other disease that can cause demyelination or
similar neurologic symptoms.
117. Laboratory Diagnosis
Cerebrospinal fluid (CSF) is tested for levels of certain
immune system proteins and for the presence of
oligoclonal bands.
These bands indicate an abnormal autoimmune
response within the central nervous system, meaning
the body is producing an immune response against
itself.
Oligoclonal bands are found in the spinal fluid of about
90-95% of people with MS, but since they are present
in other diseases as well, they cannot be relied on as
positive proof of MS. They may also take some years to
develop.
119. Treatment
The treatment of MS focuses mainly on
decreasing the rate and severity of relapse,
reducing the number of MS lesions, delaying the
progression of the disease, and providing
symptomatic relief for the patient.
Several different drugs have been developed to
treat the symptoms of MS.
Drug treatment depends on the stage of the
disease as well as other factors.
120. Myasthenia Gravis
It is a chronic autoimmune neuromuscular
disease characterized by varying degrees of
weakness of the skeletal (voluntary) muscles of
the body.
It is the most common primary disorder of
neuromuscular transmission
121. Symptoms
Facial weakness,
Difficulty chewing and swallowing,
Inability to maintain support of trunk, neck or
head.
122. Myasthenia Gravis
Antibody mediated damage to acetylcholine
receptors in skeletal muscles leading
toprogressive muscle weakness.
Acetylcholine released from nerve endings to
generate muscle contraction.
Antibody combines with receptor site, blocking
acetylcholine binding.
Receptors destroyed by action of antibody and
complement.
124. Laboratory Testing
Autoantibodies to the Acetylcholine receptor
(AChRAb) can be detected in 80-90% of
patients with myasthenia gravis.
The assay measures antibodies that precipitate
solublized muscle AChR that has been
complexed with radiolabeled alpha-
bungarotoxin (αBTX). Antibodies that bind to
the receptor regions that are not sterically
blocked by the αBTX are detected.
125. Goodpasture’s Syndrome
An uncommon and life-threatening
hypersensitivity disorder believed to be an
autoimmune process related to antibody
formation in the body.
Goodpasture's syndrome is characterized by
renal (kidney) disease and lung hemorrhage.
126. Goodpasture’s Syndrome
Antibodies react with antigens in the glomerular
basement membrane of the kidney, results in
severe necrosis.
Antigen in kidney is similar to antigen found in
lungs, resulting in antibody reacting with lung
tissue resulting in pulmonary hemorrhage.
Specific anti-basement antibodies can be
demonstrated.
127. Symptoms
Symptoms include:
foamy,
bloody, or dark colored urine,
decreased urine output,
cough with bloody sputum,
difficulty breathing after exertion,
weakness,
fatigue,
nausea or vomiting,
weight loss,
nonspecific chest pain
and/or pale skin
128. Diagnosis
Complete blood count (CBC)
Blood urea nitrogen (BUN) and creatinine levels
Urinalysis will be done to check for damage to the kidneys.
Sputum test to look for specific antibodies.
Chest x ray to assess the amount of fluid in the lung tissues.
Lung needle biopsy and a kidney biopsy will show immune
system deposits.
Kidney biopsy can also show the presence of the harmful
antibodies that attack the lungs and kidneys
Antiglomerular basement membrane (anti-GBM) antibody
Enzyme immunoassay (EIA)
Antibodies to Neutrophil Cytoplasmic Antigens (ANCA)
identified by immunofluorescence
130. Sjogren's Syndrome
Sjogren's syndrome is an autoimmune disease,
characterized by the abnormal production of extra
antibodies in the blood that are directed against various
tissues of the body.
This particular autoimmune illness is caused by
inflammation in the glands of the body.
Inflammation of the glands that produce tears (lacrimal
glands) leads to decreased water production for tears
and eye dryness.
Inflammation of the glands that produce the saliva in
the mouth (salivary glands, including the parotid glands)
leads to mouth dryness.
131. Sjogren’s Syndrome
Sjogren's syndrome classically features a
combination of dry eyes, and dry mouth .
Most often occurs secondary to RA, SLE or
other autoimmune disorders
Dry eyes and mouth due to damage to secretory
ducts.
90% of cases found in women.
133. Treatment
Nonsteroidal anti-inflammatory drugs
(NSAIDs), such as aspirin and ibuprofen
Corticosteroids
Saliva substitutes
Artificial tears or eye drops
Cyclosporine A (Restasis) eye drops
134. Scleroderma
A rare, chronic disease characterized by excessive deposits of
collagen.
Causes skin thickening and tightening, and can involve fibrosis
and other types of damage to internal body organs.
This condition, thought to be an autoimmune disease, affects
both adults and children, most commonly adult women.
he most evident symptom is the hardening of the skin and
associated scarring.
Typically the skin appears reddish or scaly in appearance. Blood
vessels may also be more visible. W
here large areas are affected, fat and muscle wastage will weaken
limbs and affect appearance.
136. Calcinosis
The buildup of calcium deposits in the tissues.
It may occur under the skin of the fingers, arms,
feet, and knees, causing pain and infection if the
calcium deposits pierce the surface of the skin.
137. Raynaud’s Phenomena
is a problem of poor blood flow to fingers and
toes.
Blood flow decreases because blood vessels in
these areas become narrow for a short time, in
response to cold or to emotional stress.
Results in: finger sensitivity, toe sensitivity cold
sensitivity, changes in skin color, finger pain, toe
pain, fingertip ulcers, toe ulcers
138. Esophageal Dysmotility
The digestive system includes the mouth,
esophagus, stomach, and bowels.
Scleroderma can weaken the esophagus and the
bowels.
It can also build-up of scar tissue in the
esophagus, which narrows the tube.
140. Telangiectasias
Telangiectasias are small enlarged blood vessels near
the surface of the skin, usually they measure only a few
millimetres.
They can develop anywhere on the body but commonly
on the face around the nose, cheeks and chin
144. Immunoproliferative Disease
B-cell immunoproliferative disorders most commonly
evaluated.
B-cell lineage develop into plasma cells
Urine antibodies used to diagnose and evaluate certain B-cell
proliferations
B-cells produce one antibody specificity (monoclonal).
Persistent presence of large amounts of a single
immunoglobulin suggests malignancy.
Increase in total amount of one specific clone characteristic
of benign reactive immunoproliferative disease.
145. Plasma Cell Dyscrasias
Include several related syndromes:
Multiple myeloma
Waldenstrom’s macroglobulinemia
Light-chain disease
Heavy-chain disease
Monoclonal gammopathy of undetermined
significance.
146. Plasma Cell Dyscrasias
Characteristic is over production of a single
immunoglobulin component.
Paraprotein or myeloma protein.
Diagnosis and monitoring dependent on
detecting and quantitating the paraprotein.
Screening and confirmatory tests performed
in most clinical laboratories.
147. Multiple Myeloma
Malignancy of mature plasma cells.
Most serious and common of plasma cell dyscrasias.
Age of diagnosis 40 t0 70 years, found in blacks
twice as frequently as whites, and men twice as likely
as women.
Have excess of plasma cells in the bone marrow.
Level of normal immunoglobulin decreased in
proportion to abnormal immunoglobulin.
148. Multiple Myeloma
Immunoglobulin produced by malignant clone,
can be of any class, IgG most common.
Important diagnostic feature is presence of
Bence Jones protein in the urine.
Abnormal production of free immunoglobulin light
chains, kappa or lambda.
Can be detected by immunoelectrophoresis or heat
precipitation.
149. Clinical Manifestations
Hematologic related to failure of bone marrow to produce
normal number of hematoopoeitic cells, leads to anemia,
thrombocytopenia and neutropenia
High levels of immunoglobulins lead to rouleaux formation being noted
on blood smear.
High levels of abnormal plasma cells leads to deficiency in normal
immunoglobulin levels.
Myeloma involves bone leading to lytic lesions, bone pain and fractures.
Deposition of antibody derived material leads to organ dysfunctions, with
kidneys most commonly involved.
Hyperviscosity develops when protein levels are high, especially with IgM
producing tumors.
Hemorrhage can occur due to thrombocytopenia and paraprotein
interferes in normal hemostasis.
150. Waldenstrom’s Macroglobulinemia
Malignant proliferation of IgM producing lymphocytes
Malignant cells more immature than plasma cells, with
appearance being between small lymph and plasma cell.
Plasmacytoid lymphs infiltrate bone marrow, spleen and
lymph nodes.
Some IgM paraproteins behave as cryoglobulins,
precipitate at cold temperatures.
Occlude small vessels in patient’s extremities in cold weather.
Leads to skin sores and necrosis of fingers and toes.
151. Waldenstrom’s Macroglobulinemia
Cryoglobulins detected in blood or plasma by placing
the sample in a refrigerator in the clinical laboratory.
Precipitate forms at low temperatures.
Dissolves upon rewarming.
May be associated with a cold red cell autoantibody directed
against the I antigen on the patient’s own red blood cells, may
result in hemolytic anemia.
Patients with stable production of monoclonal IgM
without infiltration of marrow or lymphoid tissue are
considered to have cold agglutinin syndrome.
152. Clinical Symptoms
Clinical symptoms:
Anemia
Bleeding
Hyperviscosity
Median survival 5 years versus multiple
myeloma, 3 years.
153. Laboratory Diagnosis
Measurement of immunoglobulin levels in serum.
Serum protein electrophoresis to separate and detect abnormal
levels, myelomas which produce only light chains may be missed.
Immunoelectrophoresis used to evaluate monoclonal
gammopathies detected by SPE.
Immunofixation electrophoresis also used to evaluate
monoclonal gammopathies.
Serum viscosity measurements useful for Waldenstrom’s
macroglobulinemia or high levels of IgG or IgA paraproteins.
Bone marrow biopsy to establish diagnosis of
lymphoproliferative disorder and determine extent of bone
marrow replacement by malignancy.