By Thanyamon C.
DNA


                  1
(BIOTECHNOLOGY)
            Œ           Œ
        




                                            By Thanyamon C.
Œ
                     Œ             
                     Œ Œ
    ˜                       ,
   ‹      ‹ ,                   ,
DNA Technology                          2
(GENETIC ENGINEERING)
      Œ           DNA     Œ recombinant DNA
Recombinant DNA       Œ DNA         š     ‹




                                             By Thanyamon C.
   DNA      Œ      Œ Œ               Œ




                                         3
           (RESTRICTION ENZYME)
Hamilton Smith                   ŒŒ                   
       š          ˜          Œ                    




                                                                  By Thanyamon C.
            1,200
                         ‹                    ‹           ‹
      Œ          ‹
              5’ → 3’
                   Œ Œ                    2
                ‹ DNA                 Œ
DNA ‹                                 (sticky end)
  ‹Œ                             ‹ (blunt end)                4
By Thanyamon C.
                      5

By Thanyamon C.
                      6
‹
             (DNA LIGASE ENZYME)
     š        Œ            ‹   DNA
 ‹                 Œ             ‹ 2




                                                     By Thanyamon C.
   DNA Œ ‹          Œ
 ‹        DNA              ‹                 ‹
    Œ Œ                         Œ
     Œ restriction enzyme
        ‹        DNA      Œ DNA
(Recombinant DNA)                                7
RECOMBINANT DNA




                      By Thanyamon C.
                  8
(CLONE)
                 DNA Œ
Œ        ‹                Œ‹




                                   By Thanyamon C.
      DNA (DNA cloning)
DNA
    (gene cloning)
DNA           š
                               9
(plasmid)        DNA     š                       ‹       ‹




                                                                            By Thanyamon C.
DNA             (vector)               DNA                     Œ
      ‹                      DNA
                              
                            DNA        Œ           Œ                   Œ
                                               Œ       ‹                   10

           ŒŒ      ‹         Œ             Œ
By Thanyamon C.
                                11
DNA CLONING
PCR
PCR (polymerase chain reaction)
  ‹ DNA        Œ                        ‹         




                                                       By Thanyamon C.
             Œ   ˜                  ‹ thermocycler
       š                 Œ                      Œ
            Œ           DNA polymerase     Œ Œ
   Œ                 ‹     Œ                          12
PCR ( ‹ )
                  Œ       Œ
DNA   ‹       š       ‹        DNA       Œ




                                                     By Thanyamon C.
       (primer) š DNA
 ‹     DNA Œ          š              Œ         Œ
DNA
Nucleotide 4                  ATCG
DNA polymerase
                             pH Œ                  13
By Thanyamon C.
                  14
By Thanyamon C.
                        15
PCR
PCR ( ‹ )
                         PCR
                                          DNA           Œ         DNA            Œ




                                                                                    By Thanyamon C.
    Œ                 Œ                                      (‹              Œ   PCR
‹           Œ                 Œ                             ‹)
 Œ                                                                     Œ
DNA                                         Œ
                DNA                   Œ             ‹
                          ‹
                DNA               ‹             
                                                                                   16
 DNA




                                                   By Thanyamon C.
Gel electrophoresis                  DNA
    ‹               Œ       ‡           ‹      š
  ‹ Œ ‹ agarose gel       polyacrylamide gel
DNA         ( -)        Œ        (anode)       17

DNA              ‹         Œ‹       ‹ Œ Œ ‹
 DNA ( ‹ )
DNA ‹                      ‹
   Œ Œ Œ Œ      ethidium
bromide




                                    By Thanyamon C.
DNA Œ                ‹         Œ

    Œ     DNA Œ       Œ
            DNA
    (DNA molecular weight
marker)      Œ                     18

      DNA      Œ
 DNA ( ‹ )
Automated sequence                Œ                   
    Œ gel electropholysis ‹   Œ       




                                           By Thanyamon C.
                                          19
(GENOME)
     (genome)      Œ
                                      Œ
 ‹   š gene            ‹   ‹ ‹ gene




                                                      By Thanyamon C.
Genome ‹ š                
                  ‹ DNA Œ
restriction enzyme Œ
 Œ     gel electropholysis          Œ Genome
         DNA                                     ‹
Restriction fragment length                  ‹
polymophism (RFLP)                DNA          ‹     20

               Œ restriction enzyme
(‹)
 Human Genome Project
               š




                           By Thanyamon C.
                  
                   autosome
    sex chromosome
 Human Genome Project

          ‹               21
                                             
HUMAN GENOME PROJECT




                        By Thanyamon C.
                       22
 Œ                                                   DNA
             Œ                               
                                ‹                            ‹         HIV




                                                                                       By Thanyamon C.
                                                  ‹                               š
(       Œ                                                                     ‹
                      
                      )
          Œ                               Œ                         (gene therapy)
    ‹     SCID š                     Œ†               ‹             Œ Œ       Œ
        Œ                                                ‹                     ,
                          HIV,
                          HIV,                                                        23
By Thanyamon C.
                                 24
GENE THERAPY
By Thanyamon C.
                  25

 Œ                  DNA
                Œ                  
          




                                                            By Thanyamon C.
                    
                              Œ
      DNA (DNA fingerprint)                  DNA
(DNA band) š           ‹              DNA š             
         (Œ ‹ ‹     Œ               DNA ‹          )   26
By Thanyamon C.
                      27


By Thanyamon C.
                      28


By Thanyamon C.
                        29
Œ
DNA
 Œ                    DNA ( ‹ )
                  Œ
GMOs (Genetically Modified Organisms)
                           Organisms)




                                                             By Thanyamon C.
                                  molecular breeding
                                                       (
                       ‹       Œ    )
    ‚    Š                 ‹

                                                            30
  Œ
        ‹                 
            Œ    ,      Œ 




                                   By Thanyamon C.
                 (transgenic
animal),
animal),             
      (in vitro fertilization)
<Œ             ‹             Œ>
                                  31
‹ TRANSGENIC ANIMAL




                       By Thanyamon C.
                      32
Œ                                                       (transgenic plant) ‹
    Œ                             Œ                                ‹ ‡ BT           ‹
Œ                                                                             Œ   Œ        
    ‹                 ‡ , Œ                   ,                ,     ,




                                                                                                By Thanyamon C.
        Œ               Œ             ‹                   ‹                 Œ
          Œ                               Œ                 ‹
            Œ                 ‹                                        ‹ Œ      (       A)
                  Œ                                             Œ         ‹           Œ
      Œ                                                 ‹               Œ
              Œ
                Œ                                 ‹ ‹                                          33
By Thanyamon C.
                         34
GMOS
‹
 Œ                           DNA ( ‹ )
        Œ                          Œ    Œ                       Œ
    Œ                 (mutant)         Œ                                    Œ ‹




                                                                                       By Thanyamon C.
                   ‚      Š     ‹
                (bioinformation)                            ŒŒ
                 Œ                Œ                     Œ                          Œ
            Œ                 Œ           Œ                                  Œ ‹
Œ                        ‹ Œ                     , Œ                                  ,
                            ‹               Œ       ‹                Œ
                                     Œ                                   Œ         35

              Œ                     Œ ‹
 Œ                   DNA ( ‹ )
                  Œ                Œ
Œ                                        ‹        ‹




                                                         By Thanyamon C.
           „œ
        ‹ ‹             ‹       ‹



                                                        36
DNA

                                       Œ                      ‹




                                                                    By Thanyamon C.
    ‹ Œ                   Œ    Œ                    ‹    
                          Œ    Œ              
            Œ            GMOs Œ                    ‹ š        GMOs
Œ               Œ
                    ŒŒ        genome                          Œ
          ‹ Œ            ŒŒ    ‹ Œ         Œ                       37
By Thanyamon C.
                  38
By Thanyamon C.
                     39
                  The End

Microsoft power point พันธุศาสตร์และเทคโนโลยีทาง dna

  • 1.
  • 2.
    (BIOTECHNOLOGY) Œ Œ  By Thanyamon C. Œ  Œ   Œ Œ ˜ , ‹ ‹ , , DNA Technology 2
  • 3.
    (GENETIC ENGINEERING) Œ DNA Œ recombinant DNA Recombinant DNA Œ DNA š ‹ By Thanyamon C. DNA Œ  Œ Œ Œ 3
  • 4.
     (RESTRICTION ENZYME) Hamilton Smith ŒŒ  š ˜ Œ  By Thanyamon C. 1,200 ‹ ‹ ‹ Œ ‹ 5’ → 3’ Œ Œ 2 ‹ DNA Œ DNA ‹ (sticky end) ‹Œ ‹ (blunt end) 4
  • 5.
  • 6.
  • 7.
     (DNA LIGASE ENZYME) š  Œ ‹ DNA ‹ Œ  ‹ 2 By Thanyamon C. DNA Œ ‹ Œ ‹ DNA ‹ ‹ Œ Œ Œ Œ restriction enzyme ‹ DNA Œ DNA (Recombinant DNA) 7
  • 8.
    RECOMBINANT DNA By Thanyamon C. 8
  • 9.
    (CLONE) DNA Œ Œ ‹ Œ‹ By Thanyamon C. DNA (DNA cloning) DNA (gene cloning) DNA š 9
  • 10.
    (plasmid) DNA š ‹ ‹ By Thanyamon C. DNA (vector) DNA Œ ‹ DNA  DNA Œ Œ Œ Œ ‹ 10 ŒŒ ‹ Œ Œ
  • 11.
    By Thanyamon C. 11 DNA CLONING
  • 12.
    PCR PCR (polymerase chainreaction) ‹ DNA Œ ‹  By Thanyamon C. Œ ˜ ‹ thermocycler š Œ Œ  Œ DNA polymerase Œ Œ Œ ‹ Œ 12
  • 13.
    PCR ( ‹) Œ Œ DNA ‹ š ‹ DNA Œ By Thanyamon C.  (primer) š DNA ‹ DNA Œ š Œ Œ DNA Nucleotide 4 ATCG DNA polymerase  pH Œ 13
  • 14.
  • 15.
  • 16.
    PCR ( ‹)  PCR DNA Œ DNA Œ By Thanyamon C. Œ Œ (‹ Œ PCR ‹ Œ Œ ‹) Œ  Œ DNA Œ DNA Œ ‹ ‹ DNA ‹  16
  • 17.
     DNA By Thanyamon C. Gel electrophoresis DNA ‹ Œ ‡ ‹ š ‹ Œ ‹ agarose gel polyacrylamide gel DNA ( -) Œ (anode) 17 DNA ‹ Œ‹ ‹ Œ Œ ‹
  • 18.
     DNA (‹ ) DNA ‹ ‹ Œ Œ Œ Œ ethidium bromide By Thanyamon C. DNA Œ ‹ Œ Œ DNA Œ Œ DNA (DNA molecular weight marker) Œ 18 DNA Œ
  • 19.
     DNA (‹ ) Automated sequence Œ  Œ gel electropholysis ‹ Œ  By Thanyamon C. 19
  • 20.
    (GENOME) (genome) Œ Œ ‹ š gene ‹ ‹ ‹ gene By Thanyamon C. Genome ‹ š  ‹ DNA Œ restriction enzyme Œ Œ gel electropholysis Œ Genome DNA ‹ Restriction fragment length ‹ polymophism (RFLP) DNA ‹ 20 Œ restriction enzyme
  • 21.
    (‹) Human GenomeProject  š By Thanyamon C.    autosome sex chromosome Human Genome Project ‹ 21 
  • 22.
    HUMAN GENOME PROJECT By Thanyamon C. 22
  • 23.
     Œ DNA  Œ   ‹ ‹ HIV By Thanyamon C. ‹ š ( Œ ‹  ) Œ Œ (gene therapy) ‹ SCID š Œ† ‹ Œ Œ Œ Œ  ‹  , HIV, HIV, 23
  • 24.
    By Thanyamon C. 24 GENE THERAPY
  • 25.
  • 26.
     Œ DNA  Œ   By Thanyamon C.    Œ  DNA (DNA fingerprint) DNA (DNA band) š ‹ DNA š  (Œ ‹ ‹ Œ  DNA ‹ ) 26
  • 27.
  • 28.
  • 29.
  • 30.
     Œ DNA ( ‹ )  Œ GMOs (Genetically Modified Organisms) Organisms) By Thanyamon C.  molecular breeding  ( ‹ Œ ) ‚ Š ‹ 30
  • 31.
      Œ ‹   Œ , Œ  By Thanyamon C. (transgenic animal), animal),  (in vitro fertilization) <Œ ‹ Œ> 31
  • 32.
    ‹ TRANSGENIC ANIMAL By Thanyamon C. 32
  • 33.
    Œ (transgenic plant) ‹ Œ Œ ‹ ‡ BT ‹ Œ Œ Œ  ‹ ‡ , Œ , , , By Thanyamon C. Œ Œ ‹ ‹ Œ Œ Œ ‹ Œ ‹ ‹ Œ ( A) Œ Œ ‹ Œ Œ ‹ Œ Œ Œ ‹ ‹ 33
  • 34.
    By Thanyamon C. 34 GMOS ‹
  • 35.
     Œ DNA ( ‹ ) Œ  Œ Œ Œ Œ  (mutant) Œ Œ ‹ By Thanyamon C. ‚ Š ‹ (bioinformation) ŒŒ Œ Œ Œ Œ Œ Œ Œ Œ ‹ Œ  ‹ Œ , Œ , ‹ Œ ‹ Œ Œ Œ 35 Œ Œ ‹
  • 36.
     Œ DNA ( ‹ )  Œ Œ Œ   ‹ ‹ By Thanyamon C.  „œ ‹ ‹ ‹ ‹ 36
  • 37.
    DNA Œ  ‹ By Thanyamon C. ‹ Œ Œ Œ ‹   Œ Œ  Œ GMOs Œ ‹ š GMOs Œ Œ ŒŒ genome  Œ ‹ Œ ŒŒ ‹ Œ Œ 37
  • 38.
  • 39.
    By Thanyamon C. 39 The End