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13

     35
5.
DNA
  ?
DNA
Deoxyribonucleic Acid



DNA

                        DNA
DNA



    JAMES D.WATSON   FRANCIS H.C.
CRICK
                       DNA
DNA
-    Adenine       A
-    Guanine      G
-   Thymine       T                 A
-      Cytosine        CT , C   G
       P
DNA
                 DNA
polynucleotide   2
                 TAGC
                 Deoxyribose
20
              mRNA
                  4
ATC       U       4
      3       1
          1
AUG

         mRNA
      UAA , UAG   UGA
DNA replication

          DNA

            replication
DNA
          (DNA REPLICATION)

                       S


DNA(DNA REPLICATION)               SEMI-
CONSERVATIVE               DNA


          RNA      3
                 DNA
DNA
DNA replication
DNA

  DNA template
DNA Gyrase    DNA
2. Enzyme Helicase            H-
bond
        ATP                   SSB
       polynucleotide
DNA
DNA Primase         RNA Primer
        Leading strand

DNA polymerase
 DNA               nucleotide
RNA primer         A         T
 C       G
DNA

5. RNA Primer


DNA Polymerase          nucleotide


                 Lagging Strand   DNA Ligase


                 ATP
Polynucleotide

-   Leading Strand
’   ’
-       Lagging Strand          RNA Primer
                     ’      ’
                         Okazaki fragment”
                         Polynucleotide
        ’      ’
Transcription
                DNA
RNA polymerase
DNA                   RNA
 DNA 2
DNA
RNA polymerase

 RNA polymerase
       DNA
mRNA
Translation (          )
     Translation
                mRNA
                 polypeptide


1.

2.

3.
1.
     mRNA

           tRNA
     AUG
1.tRNA
         mRNA

                tRNA
UAA,UAG,UGA          tRNA

                tRNA

mRNA
•             Gregor
    Mendel)
•



•




    Chromosome)
•

                     2




    (haploid cell)
•
2   2
-
        cytoplasm



    -       cytoplasm



-



-
-




-
-


                   egg


-      cytoplasm




    cytoplasm
Down’s syndrome
•


•
•
•
Edward’s syndrome



-

-

-
Patau’s syndrome


•

•
•
•
Cri-du-chat syndrome   Cat-cry
syndrome
•




•
•
X
Y



        Klinefelter’s syndrome
    X
           XXY
•
•
Turner’s syndrome
     X
XO
-


-

-
3.Triple X syndrome Super Female         X
                                       XXX




•

•
4.Double Y syndrome Superman         Y
                               XYY




•
•
•
•
•
•

•
•
•
Albino
•
•



            X
        =
    =
:1
•       2
    -
            2


    -
•              Clor blindness)




                                 Red –
    Green blindness)


                                     X
•       =
        =




    •
    •
Sex Determination)
                          2

1.

2.
1.
               3
     1.   XY
     2.   XO
     3.   ZW
1.   XY



      Y         Y
                        Testis)
      Y             X

-              XY
-         XX
2.     XO
     Orthoptera)
        Hemiptera)
                          X

              XX
-                    XO
-              XX
3.       ZW

                           ZW
              ZZ
     2
              Z        W
                   Z
2.

     Hymennoptera)

-                          n)
-                    2n)
               16               32
1.
-    XY       XY    XX

                              ,
-    XO       XO    XX                    ,
          X                       ,
-    ZW       ZZ(XX) ZW(XY)
                                           ,
                                          ,

                                  ,

                                      ,
(mutation)




                                     2

-   (gene mutation)
-          (chromosomal mutation)
•         Gene mutation)

            (A, T, C, G)


    DNA
(
(chromosomal
    mutation)
                   2
-
-
• 1.1
 -             aneuploidy)
              46             47
        45
             momosomic)
          disomic)
           trisomic)
            polysomic)                            21
         1                    47   trisomic-21)
-         euploidy)

       haploid=n)
        monoploid = n)
    diploid = 2n)
       triploid = 3n)
       polyploid =       n)
1.2
      (deletion)

                                   5




•            duplication)
•            inversion)
•                 translocation)
(mutagen)
1.     (radiation)                          2
-Ionizing Radiation             ,       ,
-Non-Ionizing Radiation

2.
-                (colchicine)

-

***
  (carcinogen)                      (aflatoxins)
2
1.   (Somatic cell)

2.   (Sex cell)
•   Genetic Engineering




                     insulin

        insulin
•                   DNA

                                DNA
                     DNA                            DNA
                    DNA
                                                   DNA

• DNA                                 DNA    DNA vector
                          DNA               DNA
              DNA                              DNA
              DNA
    Plasmid
•                                                     Restriction enzyme

                               DNA
                      rDNA

•         . .2513                    Hamilton Smith
              DNA
                    DNA
                                     Restriction enzyme
•                                                                   DNA
               5’         3’
•                                                               DNA
              Blunt end                                          Stricky
    end
DNA Ligase Enzyme
•                   DNA

    2       DNA
• 1.
                          DNA

• 2.                  DNA             DNA
              DNA
                     DNA
  DNA

• 3.    DNA

                                DNA
                    DNA
        DNA
•            DNA

                              DNA
            DNA



    GMO-genetically modified organism
BT
BT
-   -
-
-   -

-
-   -
Cloning



“   ”       2539
DNA
   DNA cloning)         DNA
                              gene
cloning)
•

•


•



•
•
งานนำเสนอ2

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