CRISPER-cas.9.pptx
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CRISPR-Cas9 is a genome editing method that uses a Cas9 enzyme to precisely cut DNA at a targeted location guided by a short RNA molecule. It works by integrating fragments of viral DNA into the host's genome to develop immunity. When the same virus later infects the host, CRISPR-Cas9 can recognize and cut the viral DNA. The method involves designing an RNA guide sequence to target a specific gene, with the Cas9 enzyme cutting the DNA 3-4 bases before a protospacer adjacent motif sequence. Potential applications of CRISPR-Cas9 genome editing include correcting genetic defects and developing therapies.
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CRISPR/Cas9 is a prokaryotic immune system that confers resistance to foreign genetic elements. It has been modified to edit genomes by delivering the Cas9 nuclease complexed with a guide RNA to cut DNA at a desired location. The system proceeds in three stages: adaptation incorporates new spacers into the CRISPR array, crRNA biogenesis processes the pre-crRNA, and target interference uses the crRNA-Cas9 complex to degrade invading DNA. CRISPR/Cas9 provides a simple and efficient tool for gene editing but also raises ethical concerns regarding its applications.
CRISPR
The document summarizes key aspects of CRISPR genome editing technology. It describes how CRISPR uses Cas9 and guide RNA to precisely target and edit DNA sequences. It provides a brief history of CRISPR discovery and outlines its components and mechanism of action. The document also discusses several medical applications of CRISPR including treating Duchenne muscular dystrophy, beta-thalassemia, and testing for viruses. It concludes that CRISPR is a flexible and accurate gene editing tool being explored for various applications in agriculture, biotechnology, and medicine.
CRISPR Technology
CRISPR is one of the mind blowing discovery which completely change the science of microorganisms. It is am efficient tool for genome editing and make the scientist enable to treat disease. The vast application of CRISPR technology covered almost all every aspect of life ranging from individual life to commercial aspect.
Purpose:
The purpose of this webinar is to develop creative scientific thinking in youngster and make them familiar with the miricals of science discovery.
CRISPR Cas 9 TECHNOLOGY
CRISPR Cas9 is a genome editing technology that allows genetic material to be added, removed, or altered from a genome. It originated as a bacterial immune system but can now be directed to make precise edits to DNA. The technology has wide applications for gene therapy, agriculture, research, and more, but also raises ethical concerns if misused. CRISPR offers promising possibilities but also challenges that must be addressed regarding safety, accuracy, and societal effects.
PRINCIPLE OF CRISPR GENOME EDITING
Genome editing with the CRISPR-Cas9 system has become one of the major tools in modern biotechnology. This slide share discusses the fundamentals in a simple, easy to understand format.
CRISPR_cas9_tech.pptx
CRISPR cas9 technology is a genome editing technique which won the noble prize in 2021.
CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats.
Genetic Engineering, Gene editing, Advantages of CRISPR, Limitations of CRISPR and Applications of CRISPR,
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GENE TRANSFER THROUGH EMBRYO MICROINJECTION .pptx
Gene transfer through embryo microinjection is a technique used in animal breeding strategies. It involves injecting foreign DNA into a fertilized single-cell embryo using a fine glass micropipette. The DNA is injected into the male pronucleus, which generally results in a higher rate of transgenic offspring. Microinjection allows for direct introduction of a target gene into a single cell and precise integration of a limited number of gene copies. It is commonly used to produce transgenic animals and study gene function, though it is a time-consuming and technically challenging procedure that varies based on the skill of the investigator.
Micromanipulation.pptx
Micromanipulation refers to procedures performed using microscopic instruments to manipulate eggs, sperm, and embryos. This includes techniques like partial zone dissection, subzonal insemination, intracytoplasmic sperm injection, somatic cell nuclear transfer, and gene transfer through microinjection. These techniques are used to increase fertilization and pregnancy rates, overcome male infertility, produce clones and chimeras, and enable genetic modification. The first successful transfers of sheep, pig, and cattle embryos occurred in the 1950s, with continuing advances including IVF births in the 1980s and the first animal clone in 1996. However, manipulating animals also raises ethical concerns regarding animal welfare and impacts on the environment.
plagirism.pptx
Plagiarism refers to stealing and passing off the ideas or work of others as one's own. It is considered a serious academic offense that can have negative consequences. There are several types of plagiarism, including copying an entire text (global plagiarism), rephrasing ideas without citation (paraphrasing plagiarism), directly copying words (verbatim plagiarism), stitching together sources (mosaic plagiarism), reusing one's own prior work (self-plagiarism), and accidental plagiarism by improperly citing sources. Plagiarism can be avoided by properly citing sources, maintaining records of sources used, and utilizing plagiarism checker software.
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(Research Poster).pptx
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The cost of information acquisition by natural selection
Ryan Seamus McGee, Olivia Kosterlitz, Artem Kaznatcheev, Benjamin Kerr, Carl T. Bergstrom
bioRxiv 2022.07.02.498577; doi: https://doi.org/10.1101/2022.07.02.498577
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+
53.13485
−
27.82088
with a host spectroscopic redshift of
2.903
±
0.007
. The transient was identified in deep James Webb Space Telescope (JWST)/NIRCam imaging from the JWST Advanced Deep Extragalactic Survey (JADES) program. Photometric and spectroscopic followup with NIRCam and NIRSpec, respectively, confirm the redshift and yield UV-NIR light-curve, NIR color, and spectroscopic information all consistent with a Type Ia classification. Despite its classification as a likely SN Ia, SN 2023adsy is both fairly red (
�
(
�
−
�
)
∼
0.9
) despite a host galaxy with low-extinction and has a high Ca II velocity (
19
,
000
±
2
,
000
km/s) compared to the general population of SNe Ia. While these characteristics are consistent with some Ca-rich SNe Ia, particularly SN 2016hnk, SN 2023adsy is intrinsically brighter than the low-
�
Ca-rich population. Although such an object is too red for any low-
�
cosmological sample, we apply a fiducial standardization approach to SN 2023adsy and find that the SN 2023adsy luminosity distance measurement is in excellent agreement (
≲
1
�
) with
Λ
CDM. Therefore unlike low-
�
Ca-rich SNe Ia, SN 2023adsy is standardizable and gives no indication that SN Ia standardized luminosities change significantly with redshift. A larger sample of distant SNe Ia is required to determine if SN Ia population characteristics at high-
�
truly diverge from their low-
�
counterparts, and to confirm that standardized luminosities nevertheless remain constant with redshift.
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CRISPER-cas.9.pptx
- 1. Submitted By: Osama Alam Department of Bio-Technology University of Science & Technology, Bannu September 2022
- 2. Genome Editing Presented by: Osama Alam Presented to: Sir Sami Sb
- 3. CRISPR-CAS 9 Method • CRISPR Associated Nuclease •Definition It is is a unique method of genome editing that helps to find a specific bit of DNA inside a cell. It edits genes by highly precisely cutting DNA and then letting natural DNA repair processes to take over. • Clustered • Regulory • Interspaced • Short • Palindrome Repeats
- 4. CRISPR It is a short segment of DNA containing short repetitions of base sequences, involved in the defence mechanisms of prokaryotic organisms to viruses. It is found in the genomes of prokaryotic organisms such as bacteria and archaea. It helps to detect and destroy DNA from similar bacteriophages
- 5. Mechanism oWhen bacteriophage attack on bacteria the first stage of the immune response is to capture phage DNA and insert it into a CRISPR locus in the form of a spacer. Cas1 and Cas2 . oSpacer DNA +Palindrome =CRISPR Locus Array osDNA is a region of non coding and they are mostly transposan oWhen viral DNA is inserted in bacteria genome it is transcribe.
- 8. Components CAS-9 CRISPER Associated Enzyme: • It helps to precise cut in DNA double strand and shift the target gene. Guided RNA: • It helps to guide the RNA for precise cut. • They contain such sequences that are complement to DNA sequence. • It cut in such places that we need of there edition. • Trans Activating CRISPER • It helps to Guide the Guided RNA sequence.
- 10. Designing sgRNA for CRISPR Experiments
- 11. PAM sequences • Each Cas nuclease binds to its target sequence only in presence of a specific sequence, called protospacer adjacent motif (PAM). • The nuclease cuts 3-4 nucleotides upstream of the PAM sequence
- 12. How CRISPER Work i. RNA can be designed for many unique sequences of DNA. ii. Targeted DNA may be faulty. iii. Could be theraptic.
- 14. Application