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Topic 1 - BAcTERiAL cLASSiFicATioN
                   ScHEMES



20/09/12                                  1
Topic content
 Characterisation of species
 Binomial system
 Problems of classification
 Phylogenetic classification




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20/09/12   3
Characterisation
 Why?..Great number and diversity of organisms.
 Classification:
 Grouping of related organism together.
 Many m/o look alike to be classified according to the
  structure
 Literally hundreds of bacterial species are small rods
  or small cocci.




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5
Basic Shapes
Bacillus (rod-shaped)
Coccus (spherical)
Spiral
  Spirillum
  Vibrio
  Spirochete




                         Figures 4.1a, 4.2a, 4.2d, 4.4a, 4.4b, 4.4c
Arrangements




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Prokaryotic Cells: Shapes
Average size: 0.2 –1.0 µm × 2 – 8 µm
Most bacteria are monomorphic
A few are pleomorphic




                                        Figure 4.7a
The Cell Wall
Prevents osmotic lysis
Made of peptidoglycan (in bacteria)




                                       Figure 4.6
Gram-Positive Bacterial Cell
Wall




                               Figure 4.13b
Gram-Negative Bacterial Cell
Wall




                               Figure 4.13c
Gram-positive          Gram-positive
                       Cell Wall
Cell Wall
Thick peptidoglycan    Thin peptidoglycan
Teichoic acids         Outer membrane
                        Periplasmic space




                                         Figure 4.13b–c
Motile Cells
Rotate flagella to run or tumble
Move toward or away from stimuli (taxis)
Flagella proteins are H antigens
 (e.g., E. coli O157:H7)
Arrangements of Bacterial
Flagella




                            Figure 4.7
20/09/12   15
History of Binomial Nomenclature
 Father of taxonomy?
 Two name system,Latin scientific name.
 Formal system of naming organisms
 Organizing,classifying and naming organisms into
  groups-according to the specific criteria.
 How to write these names??
 Vibrio cholerae
 Staphylococcus sp
 E.coli

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Genus and species
           Genus - capitalized
           species – specific epithet.

 Example:
 Escherichia coli,E.coli

 • Information gained: shape,who discovered,where it is
     found,what disease they cause.



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General problems
 Establishing a standard names for organisms so that
  we can communicate about them
 Usually classified according to their phylogenetic or
  evolutionary, but not always easy
 Leads to introduction of a dichotomous key




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Dichotomous key
 Has paired statements describing characteristic of
  organisms
 Paired statement can only have one statement true
 Each paired statement relate to each other




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Dichotomous
    Key




              Figure 10.8
Reasons causing problems
 1. Deciding what constitutes a species – because of
    some bacteria have the similar characteristics so
    taxonomist try to tolerate the organism within
    species
 2. Deciding what constitutes a kingdom – because
    most of organism having sexually reproduction so
    have very factors affecting the variable of the
    characteristics



20/09/12                                                23
Focusing on bacterial classification
 Other organism categorized from domain to species,
  but for prokaryotic cell, start from strain to genus
 However facing problems reaching the classes and
  orders level
 Wide range of studies needed on identification and
  building up taxonomy of bacteria




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Phylogenetic hierarchy
 Grouping organism according to
  common properties that implies that a
  group of organisms evolved from
  common ancestors.
 Each species retains some
  characteristics of the ancestor.


20/09/12   MIC250 by Miss Rashidah Iberahim   26
Hierarchy
 domain – Bacteria
 kingdom – none assign for bacteria
 phylum – Proteobacteria
 class – Gamma-proteobacteria
 order – Enterobacteriales
 family – Enterobacteriacea
 genus – Eschericia
 Species – E.coli
                                       start

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Three domain system classification




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Terminologies (answer)
 Taxa – subdivisions used to classify organism:
  domain, kingdom, phylum
 Phylogeny / sytematic – the evolutionary history of a
  group of organism; phylogenetic relationships are
  evolutionary relationship
 Taxonomy – the science of the classification of
  organism




20/09/12       m                                          29
The enD
   Short lecture ,but!!!
   MAKE MORE
   revision on it….



           TQ




20/09/12                   30

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(A) 1 bacterial classification schemes new

  • 1. Topic 1 - BAcTERiAL cLASSiFicATioN ScHEMES 20/09/12 1
  • 2. Topic content Characterisation of species Binomial system Problems of classification Phylogenetic classification 20/09/12 2
  • 4. Characterisation Why?..Great number and diversity of organisms. Classification: Grouping of related organism together. Many m/o look alike to be classified according to the structure Literally hundreds of bacterial species are small rods or small cocci. 20/09/12 4
  • 5. 5
  • 6. Basic Shapes Bacillus (rod-shaped) Coccus (spherical) Spiral Spirillum Vibrio Spirochete Figures 4.1a, 4.2a, 4.2d, 4.4a, 4.4b, 4.4c
  • 8. Prokaryotic Cells: Shapes Average size: 0.2 –1.0 µm × 2 – 8 µm Most bacteria are monomorphic A few are pleomorphic Figure 4.7a
  • 9. The Cell Wall Prevents osmotic lysis Made of peptidoglycan (in bacteria) Figure 4.6
  • 12. Gram-positive Gram-positive Cell Wall Cell Wall Thick peptidoglycan  Thin peptidoglycan Teichoic acids  Outer membrane  Periplasmic space Figure 4.13b–c
  • 13. Motile Cells Rotate flagella to run or tumble Move toward or away from stimuli (taxis) Flagella proteins are H antigens (e.g., E. coli O157:H7)
  • 15. 20/09/12 15
  • 16. History of Binomial Nomenclature Father of taxonomy? Two name system,Latin scientific name. Formal system of naming organisms Organizing,classifying and naming organisms into groups-according to the specific criteria. How to write these names?? Vibrio cholerae Staphylococcus sp E.coli 20/09/12 16
  • 17. Genus and species Genus - capitalized species – specific epithet. Example: Escherichia coli,E.coli • Information gained: shape,who discovered,where it is found,what disease they cause. 20/09/12 17
  • 18. 20/09/12 18
  • 19. General problems Establishing a standard names for organisms so that we can communicate about them Usually classified according to their phylogenetic or evolutionary, but not always easy Leads to introduction of a dichotomous key 20/09/12 19
  • 20. Dichotomous key Has paired statements describing characteristic of organisms Paired statement can only have one statement true Each paired statement relate to each other 20/09/12 20
  • 21. 20/09/12 21
  • 22. Dichotomous Key Figure 10.8
  • 23. Reasons causing problems 1. Deciding what constitutes a species – because of some bacteria have the similar characteristics so taxonomist try to tolerate the organism within species 2. Deciding what constitutes a kingdom – because most of organism having sexually reproduction so have very factors affecting the variable of the characteristics 20/09/12 23
  • 24. Focusing on bacterial classification Other organism categorized from domain to species, but for prokaryotic cell, start from strain to genus However facing problems reaching the classes and orders level Wide range of studies needed on identification and building up taxonomy of bacteria 20/09/12 24
  • 25. 20/09/12 25
  • 26. Phylogenetic hierarchy Grouping organism according to common properties that implies that a group of organisms evolved from common ancestors. Each species retains some characteristics of the ancestor. 20/09/12 MIC250 by Miss Rashidah Iberahim 26
  • 27. Hierarchy domain – Bacteria kingdom – none assign for bacteria phylum – Proteobacteria class – Gamma-proteobacteria order – Enterobacteriales family – Enterobacteriacea genus – Eschericia Species – E.coli start 20/09/12 27
  • 28. Three domain system classification 20/09/12 28
  • 29. Terminologies (answer) Taxa – subdivisions used to classify organism: domain, kingdom, phylum Phylogeny / sytematic – the evolutionary history of a group of organism; phylogenetic relationships are evolutionary relationship Taxonomy – the science of the classification of organism 20/09/12 m 29
  • 30. The enD Short lecture ,but!!! MAKE MORE revision on it…. TQ 20/09/12 30