Plant tissue culture involves growing plant cells, tissues, or organs in an artificial nutrient medium under sterile conditions. It allows for the rapid production of exact genetic copies of plants and is used to regenerate whole plants from genetically modified plant cells. The key requirements for successful tissue culture are appropriate explant tissue, a suitable growth medium, aseptic conditions to prevent microbial contamination, growth regulators, and frequent subculturing to provide nutrients and remove waste. The basic steps are selection of explant tissue from a healthy mother plant, sterilization of the tissue, establishment of the explant in a culture medium, multiplication through callus formation and shoot development, and root formation through manipulation of growth regulators. Benefits include providing pathogen-free plants