This document describes the process of somatic hybridization. It involves isolating protoplasts from plant tissues using either mechanical or enzymatic methods, fusing the protoplasts from two different plant species or varieties using spontaneous or induced fusion techniques, identifying and selecting hybrid cells using various markers, culturing the hybrid cells, and regenerating hybrid plants. Advantages include transferring genes for traits like disease resistance. Limitations include poor regeneration of hybrid plants and non-viability of fused products.