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 ‘Micro-propagation has many synonyms-tissue
culture, mass propagation, in-vitro culture,
cloning.
 In vitro clonal propagation.
 Micro-propagation is the practice of rapidly
multiplying stock plant material to produce a
large number of progeny plants, using modern
plant tissue culture methods.
 Widely used for orchids, ferns, many interior
foliage plants, etc.
Clone is a plant population derived
from a single individual by asexual
reproduction.
Clonal Propagation is the
multiplication of genetically identical
individuals by asexual reproduction.
 Clonal reproduction
 Multiplication stage can be recycled many
times to produce an unlimited number of
clones
 Easy to manipulate production cycles
 Disease-free plants can be produced
Rapid clonal in vitro propagation of plants:
• From cells, tissues or organs
• Cultured aseptically on defined media
• Contained in culture vessels
• Maintained under controlled conditions of light
and temperature
Commercialization of Micro-propagation
1970s & 1980s
Murashige (1974)
Broad commercial application
Tip bud
Leaf
Axillary
bud
Internode
Root
Starting material for
micropropagation
♣ Part of plant
♣ Genotype
♣ Physiological condition
♣ Season
♣ Position on plant
♣ Size of explants
♣ Minerals
♣ Sugar
♣ Organic ‘growth factors’
♣ Growth regulators
♣ Gelling agent
♣ Other additives
♣ Temperature
♣ Moisture
♣ Light
 Stage 0-I–Establishment
◦ Selection of the explants plant
◦ Sterilization of the plant tissue
takes place
◦ Establishment to growth medium
 Stage II - Proliferation
◦ Transfer to proliferation media
◦ Shoots can be constantly divided
 Stage III – Rooting & Hardening
◦ explants transferred to root media
◦ explants returned to soil
 Organogenesis
◦ Organogenesis via callus formation
◦ Direct adventitious organ formation
 Embryogenesis
◦ Direct embryogenesis
◦ Indirect embryogenesis
 Micro-cutting
◦ Meristem culture (Mericloning)
◦ Bud culture
• From one to many propagules rapidly.
• Multiplication in controlled lab
conditions.
• Continuous propagation year round.
• Potential for disease-free propagules.
• Inexpensive per plant once established.
• Specialized equipment/facilities required.
• More technical expertise required.
• Protocols not optimized for all species.
• Plants produced may not fit industry
standards.
• Relatively expensive to set up.
• Rapid increase of stock of new varieties.
• Elimination of diseases.
• Cloning of plant types not easily propagated
by conventional methods.
• Propagules have enhanced growth features
(multibranched character)
Micro propagation

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Micro propagation

  • 1.
  • 2.  ‘Micro-propagation has many synonyms-tissue culture, mass propagation, in-vitro culture, cloning.  In vitro clonal propagation.  Micro-propagation is the practice of rapidly multiplying stock plant material to produce a large number of progeny plants, using modern plant tissue culture methods.  Widely used for orchids, ferns, many interior foliage plants, etc.
  • 3. Clone is a plant population derived from a single individual by asexual reproduction. Clonal Propagation is the multiplication of genetically identical individuals by asexual reproduction.
  • 4.  Clonal reproduction  Multiplication stage can be recycled many times to produce an unlimited number of clones  Easy to manipulate production cycles  Disease-free plants can be produced
  • 5. Rapid clonal in vitro propagation of plants: • From cells, tissues or organs • Cultured aseptically on defined media • Contained in culture vessels • Maintained under controlled conditions of light and temperature
  • 6. Commercialization of Micro-propagation 1970s & 1980s Murashige (1974) Broad commercial application
  • 8. ♣ Part of plant ♣ Genotype ♣ Physiological condition ♣ Season ♣ Position on plant ♣ Size of explants
  • 9. ♣ Minerals ♣ Sugar ♣ Organic ‘growth factors’ ♣ Growth regulators ♣ Gelling agent ♣ Other additives
  • 11.  Stage 0-I–Establishment ◦ Selection of the explants plant ◦ Sterilization of the plant tissue takes place ◦ Establishment to growth medium  Stage II - Proliferation ◦ Transfer to proliferation media ◦ Shoots can be constantly divided  Stage III – Rooting & Hardening ◦ explants transferred to root media ◦ explants returned to soil
  • 12.  Organogenesis ◦ Organogenesis via callus formation ◦ Direct adventitious organ formation  Embryogenesis ◦ Direct embryogenesis ◦ Indirect embryogenesis  Micro-cutting ◦ Meristem culture (Mericloning) ◦ Bud culture
  • 13. • From one to many propagules rapidly. • Multiplication in controlled lab conditions. • Continuous propagation year round. • Potential for disease-free propagules. • Inexpensive per plant once established.
  • 14. • Specialized equipment/facilities required. • More technical expertise required. • Protocols not optimized for all species. • Plants produced may not fit industry standards. • Relatively expensive to set up.
  • 15. • Rapid increase of stock of new varieties. • Elimination of diseases. • Cloning of plant types not easily propagated by conventional methods. • Propagules have enhanced growth features (multibranched character)