Embryo culture is a tissue culture technique that involves the sterile isolation and in-vitro growth of zygotic embryos into plantlets. It includes mature and immature embryo culture methods, which are used to develop embryos that may not survive in nature due to various factors. Key applications include plant production, studying embryo-endosperm relationships, overcoming seed dormancy, and clonal propagation.

1. EMBRYO CULTURE
AND ITS
APPLICATION
BY – DIVYA BADONI
Bsc. (H) Biotechnology 2nd year
IMSUC, Ghaziabad

2. EMBRYO CULTURE
▪ Embryo culture is a type of Tissue Culture technique.
▪ WHAT IS EMBRYO ?
Male gamete + Female gamete (fusion)
fertilization
zygote
develop
embryo

3. ▪ WHAT IS EMBRYO CULTURE ?
Deals with sterile isolation and In- vitro growth of zygotic
immature/mature embryo into a plantlet.
In Somatic Embryogenesis technique we develop the embryo
into the plant with the help of Explant.
The part we select out of an living organism from which we
make the whole plant is called as Explant.

4. METHODS OF EMBRYO CULTURE
methods of embryo culture
Mature embryo culture Immature embryo culture
OR
Embryo rescue method

5. MATURE EMBRYO CULTURE
▪ The development stage is gained by embryo.
▪ Isolation of mature embryo from ripe seeds.
▪ Used when embryo doesn’t survives in-vivo(because of
seed dormancy chemical inhibitors or mechanical
resistance).

6. IMMATURE EMBRYO CULTURE/
EMBRYO RESCUE METHOD
▪ It is still in the development stage.
▪ Isolation of immature embryo from
unripe/hybrid/sterile seed which fails to develop.
▪ Used to rescue embryo of wide crosses or distant
hybridization (inter-specific and inter-generic cross)

7. PROCEDURE OF EMBRYO CULTURE
SEED
SURFACE STERILIZATION
ISOLATION OF EMBRYO
EMBRYO KEPT IN NUTRIENT MEDIA(MS/BS/White media) simple media
for mature and complex media with high concentration of sucrose for immature embryo
organogenesis differentiation
CALLUS PLANTLETS

8. APPLICATIONS OF EMBRYOCULTURE
▪ Production of plant from embryo.
▪ Study the relationship between embryo and endosperm.
▪ Prevents Embryo abortion.
▪ Overcome seed dormancy.
▪ Sterile seed due to early ripening e.g. Peach, cherry, apricot and
plum.
▪ Used in scientific study as well
▪ Clonal propagation

9. ▪ Embryo endosperm transplant.
▪ Haploid production.
▪ Biotechnology.
▪ Shortening of breeding cycle.