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Dna Sequencing

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Zahoor Ahmed
Zahoor Ahmed

The document summarizes DNA sequencing methods. It discusses the DNA double helix structure and how the four nitrogenous bases form complementary pairs between strands. It then describes the two main historical DNA sequencing methods: the Maxam-Gilbert method which uses chemical degradation, and the Sanger method which is based on chain termination using dideoxynucleotides. The Sanger method is now the most common approach and involves sequencing in four separate reactions with one of the four ddNTPs added to each.

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[object Object],[object Object],[object Object],DNA SEQUENCING
DNA ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Watson & Crick Model   ,[object Object],[object Object],[object Object],[object Object],[object Object]
DNA SEQUENCING ,[object Object]
PURPOSE ,[object Object],[object Object],[object Object],[object Object],[object Object]
DNA SEQUENCING METHODS ,[object Object],[object Object],[object Object],[object Object]
MAXAM & GILBERT METHOD ,[object Object],[object Object]
PRINCIPLE : ,[object Object],[object Object],[object Object],[object Object]
 
 
SANGER METHOD ,[object Object],[object Object],[object Object],[object Object]
SANGER METHOD TERMED AS CHAIN TERMINATION METHOD ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
DEOXY VERSUS DIDEOXY
PRINCIPLE : ,[object Object],[object Object],[object Object],[object Object]
REQIREMENTS ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
HOW DOES THE DNA TEMPLATE OBTAINED? ,[object Object],[object Object],[object Object]
 
COMPARISON Automation is not available Automation Double-stranded or single- stranded DNA Single-stranded DNA. ,[object Object],[object Object],[object Object],Requires DNA synthesis ,[object Object],[object Object],Maxam Gilbert Method Sanger Method
Next Generation Technologies: ,[object Object],[object Object],[object Object],[object Object]
THANK YOU

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Dna Sequencing

Editor's Notes

  1. The template for a chain termination experiment is a single-stranded version of the DNA molecule to be sequenced. There are several ways in which this can be Obtained;