DNA sequencing is a fundamental process in biotechnology that determines the order of nucleotides in a DNA molecule. There are two main methods: the chemical method developed by Maxam and Gilbert, and the enzymatic method developed by Sanger and Coulson. DNA sequencing involves degrading DNA fragments into smaller pieces, sequencing the individual fragments, and ordering the fragments to determine the full DNA sequence.

1. Introduction

2.  Determination of nucleic acid sequence
in DNA molecule is basic and
fundamental requirement in
biotechnology.
 DNA sequencing is important to
understand the function of genes, and
basis of inherited disorders.
 DNA cloning and gene manipulation
invariably require knowledge of
accurate nucleotide sequence.

3.  There are two DNA sequencing
methodologies were developed .
1. Chemical method by Alan Maxam and
Walter Gilbert of Harvard University.
2. Enzymatic method by Sanger and A. R.
Coulson of medical council in
Cambridge, England.

4.  DNA sequencing is the process of
reading the nucleotide bases in a DNA
molecule.
 This method determine the order of the
four bases
1) Adenine
2) Guanine
3) Cytosine
4) Thymine
in the strand of DNA.

5. It involves
1. The specific degradation and fractionation of
the polynucleotide of interest to fragments
small enough to be fully sequenced.
2. The sequencing of individual fragments.
3. The ordering of fragments by repeating the
preceding steps using a degradation
procedure

6. Please see next video for types of nucleic
acid sequencing