The document provides instructions for drawing blood from donors. It states that donors must be healthy and comfortable, and the skin where blood will be drawn must be thoroughly cleaned. It also notes that blood is drawn into bottles or bags containing an anticoagulant solution to prevent clotting, and several safety and quality control procedures are followed before and after drawing blood.

1. DRAWING OF BLOOD
 DONOR MUST BE HEALTHY
 ALWAYS MAKE THE DONOR TO LIE DOWN
 MORE COMFORTABLE THE DONOR
 REASURE THE DONOR KNOWS ABOUT THE PROCEDURE,
ESPECIALLY IF THIS IS THE FIRST TIME
 ASEPSIS IS IMPORTANT
08-Jan-18
COLLECTION
,PRESERVATION
AND
STORAGE
IN
BLOOD
BANK
G.KALAIVANI
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2. DRAWING OF BLOOD
• SKIN OF THE ANTECUBITAL FOSSA OF EITHER ARM MUST BE
CLEANED THOROUGHLY
• WITH TINCTURE OF IODINE FOLLOWED BY CLEANING WITH
SPIRIT
• REMOVES THE DIRT FROM THE SKIN l like OILS ,IODINE
• IF A PERSON IS ALLERGIC TO IODINE ,GETS A SKIN REACTION
WHEN IT IS USED
• ALLOW THE SKIN TO DRY BEFORE INSERTING THE NEEDLE
08-Jan-18
COLLECTION
,PRESERVATION
AND
STORAGE
IN
BLOOD
BANK
G.KALAIVANI
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3. DRAWING OF BLOOD
• BLOOD FLOWS BY GRAVITY DOWNWARDS THROUGH THE TUBE
INTO THE BOTTLE OR BAG
• AN AIRWAY IS REQUIRED IN THE BOTTLE TO ALLOW THE AIR
ALREADY IN THE BOTTLE TO ESCAPE AS THE BLOOD FILLS THE
BOTTLE
• AIRWAY IS NOT NEEDED WITH PLASTIC BAGS, CONTAIN ONLY THE
PRESERVATIVE SOLUTION
• CAN EXPAND AS THEY FILL THE BLOOD,WITHOUT DISPLACEMENT
OF ANY AIR
08-Jan-18
COLLECTION
,PRESERVATION
AND
STORAGE
IN
BLOOD
BANK
G.KALAIVANI
3

4. DRAWING OF BLOOD
• LARGE NEEDLE ON THE DONOR SET 15 OR 16 GAUGE NEEDLE
• NEEDLE MAY CAUSE SOME DISCOMFORT
• INSERTED ONLY AFTER A SMALL AMOUNT OF LOCAL
ANAESTHETIC (XYLOCAINE) IS INSERTED INTO THE SKIN
• CARE MUST BE TAKEN TO ENSURE THAT THE BOTTLE OF
LOCAL ANESTHETIC DOES NOT BECOME CONTAMINATED
08-Jan-18
COLLECTION
,PRESERVATION
AND
STORAGE
IN
BLOOD
BANK
G.KALAIVANI
4

5. DRAWING OF BLOOD
• 420ml OF BLOOD +80 ml of ACD solution = 500 ml ‘unit ‘ of
ACD BLOOD FOR TRANSFUSION
• 300 ml or 250 ml ‘UNITS’ = AVERAGE INDIAN BLOOD DONOR
IS SMALLER
• LOWER HAEMOGLOBIN THAN THE WESTERN COUNTRIES
• ONLY REASONABLE TO TAKE A SMALLER QUANTITY OF BLOOD
FROM EACH DONOR
08-Jan-18
COLLECTION
,PRESERVATION
AND
STORAGE
IN
BLOOD
BANK
G.KALAIVANI
5

6. DRAWING OF BLOOD
• HEALTHY INDIAN DONORS CAN DONATE 500 ml ‘UNIT’
WITHOUT TROUBLE
• WHILE THE BLOOD IS BEING DRAWN,THE BOTTLE SHOULD
OCCASIONALLY BE SWRILED GENTLY (DO NOT DISTURB THE
NEEDLE) IN ORDER TO ASSURE ADEQUATE MIXING OF THE
BLOOD WITH THE ANTICOAGULANT SOLUTION
08-Jan-18
COLLECTION
,PRESERVATION
AND
STORAGE
IN
BLOOD
BANK
G.KALAIVANI
6

7. DRAWING OF BLOOD
• FLOW IS USUALLY HASTENED BY
• (1) HAVING A BLOOD PRESSURE CUFF ON THE ARM ABOVE
THE NEEDLE,INFLATED TO A PRESSURE 40 -50 mm Hg
• (2) ASKING THE DONOR TO GENTLY FLEX THE FINGERS TO
ASSIST THE FLOW OF BLOOD INTO THE VEIN FROM WHICH IT
IS BEING TAKEN
08-Jan-18
COLLECTION
,PRESERVATION
AND
STORAGE
IN
BLOOD
BANK
G.KALAIVANI
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8. DRAWING OF BLOOD
• WHEN THE AMOUNT OF THE DONATION HAS BEEN
COLLECTED, BEFORE THE NEEDLE IS WITHDRAWN FROM THE
VEIN,DRAW THE LOWER NEEDLE OF THE SET FROM THE
BOTTLE AND PUT 5-10 ml SAMPLES OF THE BLOOD INTO 2 r 3
SMALL DRY BOTTLES OR TUBES
• LABELLED WITH THE SAME NUMBER AS THAT ON THE LARGE
BLOOD TRANSFUSION BOTTLE OR BAG – PILOT BOTTLES
08-Jan-18
COLLECTION
,PRESERVATION
AND
STORAGE
IN
BLOOD
BANK
G.KALAIVANI
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9. DRAWING OF BLOOD
• BOTTLES OR TUBES SHOULD BE AUTOCLAVED BEFORE USE
• ENSURE THAT THEY ARE STERILE
• VARIOUS EXTRA SAMPLES OF DONOR BLOOD TO BE USED FOR
TESTING MAY BE COLLECTED IN SEGMENTS OF EXCESS PLASTIC
TUBING PROVIDED WITH THE BAG
• RELEASE THE BLOOD PRESSURE CUFF AND WITHDRAW THE
NEEDLE FROM THE VEIN
• PRESS FIRMLY OVER THE AREA WITH COTTON,PREFERABLY WITH
THE ARM HELD STRAIGHT UP IN AN EXTENDED POSITION
08-Jan-18
COLLECTION
,PRESERVATION
AND
STORAGE
IN
BLOOD
BANK
G.KALAIVANI
9

10. DRAWING OF BLOOD
• HOLE IN THE VEIN AND SKIN IS BIGGER THAN THE ONE AFTER
THE USUAL VENIPUNCTURE FOR A BLOOD TEST SAMPLE
• DO NOT RELEASE THE PRESSURE TOO SOON
• HAVE THE DONOR HOLD IT FIRMLY FOR 3- 5 MINUTES
• PUT A STERILE COVER R SEAL OVER THE TOP OF THE BOTTLE
IMMEDIATELY AFTER STERILZING THE RUBBER STOPPER
08-Jan-18
COLLECTION
,PRESERVATION
AND
STORAGE
IN
BLOOD
BANK
G.KALAIVANI
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11. DRAWING OF BLOOD
• ONCE CLOSED,THE BLOOD BOTTLE SHOULD NEVER BE
OPENED AND ENTERED UNTIL THE DOCTOR INSERTS THE
BLOOD GIVING SET TO GIVE THE BLOOD TO A PATIENT
• BE SURE THE BOTTLE IS COMPLETELY AND CORRECTLY
LABELLED – NUMBER,NAME,GROUP,TYPE,DATE OF
COLLECTION, etc
• WHEN USING BOTTLES TO COLLECT THE BLOOD,THE CORRECT
VOLUME OF BLOOD TO BE TAKEN CAN BE DETERMINED BY
THE SCALE ON THE SIDE OF THE BOTTLE
08-Jan-18
COLLECTION
,PRESERVATION
AND
STORAGE
IN
BLOOD
BANK
G.KALAIVANI
11

12. DRAWING OF BLOOD
• IF YOU USE PLASTIC BAGS TO COLLECT THE BLOOD,THERE IS
EASY AND ACCURATE WAY TO TELL THE VOLUME OF BLOOD
DRAWN
• UNLESS YOU USE A SIMPLE PAN SCALE AND HAVE IT SET TO
TRIP WHEN THE BAG REACHES THE CORRECT WEIGHT
• IMPORTANT NOT TO DRAW TO MUCH BLOOD FOR THE
AMOUNT OF ANTICOAGULANT WHICH IS IN THE BAG
• EXCESS BLOOD IS DRAWN THERE MAY NOT BE SUFFICIENT
ANTICOAGULATION
08-Jan-18
COLLECTION
,PRESERVATION
AND
STORAGE
IN
BLOOD
BANK
G.KALAIVANI
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13. DRAWING OF BLOOD
• DO NOT LET THE DONOR STAND UP IMMEDIATELY AFTER
DONATION
• THERE MUST BE A LITTLE TIME FOR THE BODY TO ADJUST TO
THE LOSS OF MUCH BLOOD
• BODY WILL ADJUST,BUT IT GENERALLY DOES NOT HAPPEN
IMMEDIATELY
• USUALLY 5-10 MINUTES WILL SUFFICE
08-Jan-18
COLLECTION
,PRESERVATION
AND
STORAGE
IN
BLOOD
BANK
G.KALAIVANI
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14. DRAWING OF BLOOD
• OVER 1-2 ADDITIONAL MINUTES THE DONOR FIRST SITS
UP,AND THEN STANDS UP,THERE IS MINIMAL CHANCE OF ANY
REACTION(FAINTING) DUE TO LOW BLOOD VOLUME AND
PRESSURE
• DONORS APPRECIATE TO DRINK A JUICE OR COFFEE OR A
PIECE OF CAKE OR OTHER PASTY
• SHOULD BE ENCOURAGED TO DRINK A LITTLE EXTRA WATER
OR OTHER FLUIDS DURING THE NEXT SEVERAL HOURS
• NO OTHER INSTRUCTIONS OR SPECIAL PRECAUTIONS ARE
NORMALLY NEEDED
08-Jan-18
COLLECTION
,PRESERVATION
AND
STORAGE
IN
BLOOD
BANK
G.KALAIVANI
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15. PREVIOUS YEAR QUESTIONS
NIL
08-Jan-18
COLLECTION
,PRESERVATION
AND
STORAGE
IN
BLOOD
BANK
G.KALAIVANI
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