students will be benefited

1. COMPATIBILITY TESTING – THE
CROSS MATCHING
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2. COMPATIBILITY TESTING – THE
CROSS MATCHING
 A COMPATIBILITY TEST IS ESSENTIAL BEFORE
ALL TRANSFUSIONS
 PROCEDURE USED TO DETERMINE
COMPATIBILITY OF DONOR AND RECIPIENT
BLOOD IS CALLED THE CROSS MATCH
 SHOULD BE DONE IN 2 PARTS CALLED THE
MAJOR CROSS MATCH AND MINOR CROSS
MATCH
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3. TESTS ARE NECESSARY FOR A SAFE
TRANSFUSON
 1.ACCURATE TESTING OF ABO AND Rh TYPING
OF BOTH THE DONOR AND RECIPIENTS DONE
 2.SCREENING TEST OF THE SERA OF BOTH
THE RECIPINET AND DONOR FOR DETECTION
OF ANY IRREGULAR ANTIBODIES
 3.SCREENING FOR HIV, HBV, HCV, MALARIA,
SYPHILIS, etc
 4.CROSS MATCHING TEST
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4. DIFFERENT METHOD
 1.SALINE CROSS MATCH
OPEN SLIDE METHOD
SALINE TUBE METHOD
IMMEDIATE –SPIN AND THERMAL INCUBATION
MODIFICATION
 2. ALBUMIN TUBE METHOD
 3.COOMB’S CROSS MATCHING
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5. SALINE CROSS MATCH
1.OPEN SLIDE METHOD
PROCEDURE
 MAKE 5% SUSPENSION IN SALINE OF THE
PATIENTS’S CELLS AND DONORS CELLS
 TAKE A CLEAN GLASS SLIDE
 MARK LEFT SIDE ONE CIRCLE & LABEL IT AS
‘P’- MAJOR SIDE
 MARK RIGHT SIDE ONE CIRCLE & LABEL IT AS
‘D’ – MINOR SIDE
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6. OPEN SLIDE METHOD
 MAJOR SIDE – 1 DROP OF THE PATIENT’S
SERUM & 1 DROP OF THE DONOR’S CELL
SUSPENSION
 MINOR SIDE – 1 DROP OF THE DONOR’S
SERUM &1 DROP OF THE PATIENT’S CELL
SUSPENSION
 MIX BY GENTLY ROTATING THE SLIDE
 INCUBATE AT ROOM TEMPERATURE FOR 10 -
15 MINUTES
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7. OPEN SLIDE METHOD
 EXAMINE BOTH MACROSCOPICALLY AND
MICROSCOPICALLY FOR AGGLUTINATION
 THEREFORE RECOMMENDED THAT TUBE
METHODS BE USED INSTEAD OF THIS SLIDE
METHOD
 ALLOWING DETECTION OF BOTH
AGGLUTINATION AND HAEMOLYSIS AS
INDICATOR OF INCOMPTABILITY
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8. SALINE TUBE METHOD
PROCEDURE
 MAKE 5% SALINE CELL SUSPENSION OF THE
DONOR AND PATIENT CELLS IN A SEPARATE
TUBE
 MAJOR CROSS MATCH TUBE – ADD 1 DROP OF
PATIENT’S SERUM AND ADD 1 DROP OF THE
DONOR CELL SUSPENSION
 MINOR CROSS MATCH TUBE – ADD I DROP OF
DONOR SERUM AND ADD 1 DROP OF
PATIENT’S CELL SUSPENSION
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9. SALINE TUBE METHOD
 MIX THE CELLS AND SERA BY GENTLY
TAPPING THE TUBES
 ALLOW TO STAND AT ROOM TEMPERATURE
FOR 30 MINUTES
 EXAMINE FOR AGGLUTINATION BOTH
MACROSCOPICALLY AND MICROSCOPICALLY
 ALSO NOTE THE COLOUR OF THE
SUPERNATANT FLUID BEFORE SUSPENDING
THE CELLS TO NOTE ANY HAEMOLYSIS
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11. SALINE TUBE METHOD
DISADVANTAGE
 METHOD REQUIRES A LONGER INCUBATION
PERIOD
ADVANTAGE
 DETECTING WEAK REACTION MORE OFTEN
 ALLOWING DETECTION OF HAEMOLYSIS
 ONLY DETECT THE AGGLUTINATING IgM
ANTIBODIES
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12. PREVIOUS YEAR QUESTION PAPER
 COMPATIBILITY TESTING (5 MARKS)
SEPTEMBER 2014/APRIL 2017/SEPTEMBER
2018
 DESCRIBE CROSS MATCHING(5
MARKS)FEB/MARCH 2012
 WHAT IS CROSS MATCHING?(3 MARKS)
AUGUST 2013
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