This document discusses the determination of red blood cell indices including packed cell volume (PCV), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), and mean corpuscular hemoglobin concentration (MCHC). It describes the macrohematocrit or Wintrobe tube method for determining PCV which involves centrifuging a blood sample and measuring the ratio of packed red cells to total blood volume. Formulas are provided for calculating MCV from PCV and RBC count, MCH from hemoglobin and RBC count, and MCHC from hemoglobin and PCV. Normal ranges are listed for each index.
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Eosinophils become active when you have certain allergic diseases, infections, and other medical conditions.
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I have listed out the LE cells structure and Microscopical examinaton of LE CELLS, Difference between tart cells and le cells, clinical symptoms and diagnostic procedure.
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RBC Indices- MCV, MCH, MCHC II Blood PhysiologyHM Learnings
RBC Indices- MCV, MCH, MCHC II Blood Physiology
The slide will cover the following:
1. Introduction to RBC indices
2. Mean Corpuscular volume (MCV)
3. Mean Corpuscular hemoglobin (MCH)
4. Mean Corpuscular hemoglobin concentration (MCHC)
5. Color index (CI)
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Hematocrit (packed cell volume) is the ratio of the volume of red cells to that of the whole blood.
It indicates relative volume of red cells and plasma (e.g. in anemia red cells are reduced with corresponding reduction in the hematocrit)
Methods
Macromethod using Wintrobe tube
• Micromethod using capillary tube
• Automated analyse
The packed cell volume is a measurement of the proportion of blood that is made up of cells. The value is expressed as a percentage
The packed cell volume (PCV) is a measurement of the proportion of blood that is made up of cells. The value is expressed as a percentage or fraction of cells in blood. For example, a PCV of 40% means that there are 40 millilitres of cells in 100 millilitres of blood.
2. 1. Packed cell volume(PCV)
2. Mean corpuscular volume(MCV)
3. Mean corpuscular hemoglobin(MCH)
4. Mean corpuscular hemoglobin concentration(MCHC)
5. Color index (CI)
4. • Hematocrit literally means ‘blood separation’.
• It measures the percentage of volume of packed
red cells.
• So it is also known as Packed cell volume(PCV).
8. Wintrobe tube:
• 110 mm long, narrow, thick walled tube with 3mm
internal bore.
• Graduated from 0-10 cm with graduation both in
ascending and descending order on 2 sides of tube.
• The scale with the markings from 0-10 from above
downwards is used in ESR determination and from below
upward is used for Hematocrit (PCV) determination.
9.
10. Procedure :
1. Under aseptic condition get a venous blood sample.
2. Carefully mix the blood sample in EDTA vial by
repeated inversion.
3. Fill the Wintrobe tube with the help of Pasteur
pipette to the 10 cm mark ( which represent 100%).
4. Place the Wintrobe tube in the centrifuge machine,
and other wintrobe tube filled with water on the
opposite side so as to balance it.
5. Turn the centrifuge on to the slow speed and then
gradually increase the speed.
11. 6. Centrifuge for 30 minutes at 3000 rpm.
7. After 30 minutes stop the centrifuge machine.
8. Take out the Wintrobe tube and note the readings
directly off the graduation.
16. How MCH is calculated ?
• It is calculated from the following basic values:
I. RBC count in million/mm3
II. Hb in gm%
• Formula:
MCH = Hb in gm% X 10
RBC count
Normal range: 27 – 32 pg
