Blood components: sample collection, labeling, preservation, storage, documentation, record keeping, retention of records, quality control system.

1. By: Nityanand Upadhyay
Associate Professor
Department of MLT
Integral University, Lucknow

2.  SAMPLE COLLECTION
 PRESEVATION
 STORAGE
 LABELING
 TRANSPORT
 RECORD KEEPING
 DOCUMENTATION
 QUALITY CONTROL

3. 1. Venipunture site:
i. Inspection of the area situated in front of the
elbow (Antecubital area).
ii. Both are should be checked.
iii. Locate and select suitable vein to be
punctured.

4. 2. Use of Tourniquet:
i. A sphygmomanometer placed on the
upper arm.
ii. Inflated to 50 mm Hg.
iii. It is important to make sure not to impair
arterial flow.

5. 3. Phlebotomy:
i. Prepare an Area of about 1.5 inches in all
direction.
ii. Scrub vigorously with soap solution for 30
second ( clean away fat, oils, dirt , skin cells).
iii. Apply 10 percent acetone in 70 percent
isopropylalcohol(1+9 part) allow to dry.

6. iv. Apply Tincture of Iodine (3 percent iodine in
70 percent ethyl alcohol) and allow to dry.
v. Remove the iodine with 10 percent acetone in
70 percent isopropylalcohol.
vi. Perform Venipunture.

7. 4. Instructions:
a. Do not repalpate the vein after venipunture.
b. Blood collected in double or triple bags
containing suitable Anticoagulant.
c. The flow of blood should be rapid and without
intrupted.
d. Blood bag should be inverted several time
during Phlebotomy.(Satisfactory mixing of
anticoagulant with blood).

8. e. The collection should be completed within 8-10
mins.
f. Total Quantity are:
350ml bag: 350ml± 10% of blood.
450ml bag: 450ml± 10% of blood.
g. Collected blood store in an air condition room.

11. 1. ACD & CPD preserve the unit for 21 days at
2-6ºC.
2. CPDA-1 (anticoagulant/preservative for 35
days).
• C = Citrate→ to prevent clotting
• P = Phosphate→ to maintain pH
• D = Dextrose→ ATP generation
• A = Adenine-1→ substrate from which RBC
produce
Anticoagulant ratio is 1.4 ml:10ml blood (63ml / 450ml)

12. Additive Solution:
• (SAGM) → Saline-Adenine-Glucose- Manitol
• Purpose of additive solution, to improve RBCs
storage viability till 42 days @ 2-6ºC
• Note: Added only to PRBC,s

13. 1. PRBC : 2-60 C 35 days , 42 days with
additive solution “SAGM”
2. FFP: -180 C for 1year, at -800 C for >1
Years.
3. CPP: -300 C, 5-7 days according to bag in
use.

14. 4. Granulocytes: -200 C to -240 C
5. Platelets: 20 0 C to 24 0 C 3-5 days (with gentle
agitation).
6. CRYO: -300C for1 Year

17.  Whole blood/PRBC/FFP/Cryoprecipitate,
must be transported in Blood transport box
which contains ice packs inside the box,
 Platelets do not use freezing.

18. 1. A record keeping system must be in place to
trace significant steps in processing of Donor
blood and Recipient sample.
2. Documentation and Record form one of the
important quality system essentials in any
blood bank/blood transfusion service.

19. 3. The record keeping system may be Manual,
Computerized or a Combination of both.
4. Abbreviations and symbols must be properly
defend.
5. Documentation and Records involve every
aspect of blood banking i.e.
i. Donor recruitment
II. Donor selection

20. III. Donor recall.
IV. Blood collection.
V. Blood components processing.
VI. Storage.
VII. Transportation of blood and blood
components.

21. VIII. Screening of blood for TTI'S.
IX. Adverse reactions to blood transfusion (Blood
Transfusion Reaction).
X. Issue of and transfusion of blood and blood
component to the patient.
XI. Stock
XII. Disposal Record.
=>Ensurance of Confidentiality and Security
of the all information.

22. 1.Record of blood donors:
. Blood donor consent for donation Donor's name
and father/husband name.
. Donation - voluntary or replacement.
. Date of birth (age), gender and weight.
. Address (office & residence) and telephone number
History of illness.

23. 2. Record of blood donation:
. Date of blood donation.
. Donation number (Identification number)
3. Physical examination record :
.Pulse, temperature, and blood pressure.
. Hemoglobin ABO and Rh (D) group.
. Results of HBsAg , anti-HCV, anti-HIV 1&2.

24. . VDRL/RPR and malaria tests.
. Disposal: issued for transfusion or discarded.
“ Minimum record keeping requirements
Source: Drugs and Cosmetic Act 1940”

25. 4. Record of blood component
preparation:
. Name of component.
. Donation number.
. ABO and Rh (D) group.
. Date of preparation.
. Result of tests of markers of transmissible
infections.

26. 5. Disposal:
. Issued for transfusion or discarded.
6. Recipient requisition form:
. Patient's name with father/husband's name.
. Admission/Registration number.
. Age and Sex.
. Hospital name - room/bed number.
. Name of clinician attending the patient.

27. . Diagnosis and reasons of transfusion.
. Number of units of blood/component
required.
. Date and time of requirement.
. Requirement is routine or emergency.
“ Minimum record keeping requirements
Source: Drugs and Cosmetic Act 1940.”

28. 7. Compatibility Test Record on the
Requisition Form :
. Recipient's ABO and Rh (D) group.
. Antibody screening in patient's blood.
. Donor's unit donation number ABO and Rh
(D) of donor‘ unit.
. Cross-matching for IgM & IgG .

29. Result of compatibility :
. Compatible or not compatible.
. Initial of technician performing the test.
8. Blood/ component issue record:
. Serial number & Date.
. Patient's name, Age & Sex.
. Admission number & room / ward number.
. ABO&Rh (D) Donor's unit.

30. . Donation number.
. Date of Collection & Date of Expiry
. Name of Components.
. Compatibility for IgM & IgG.
. Cross-matched by.
. Issued by & time of issue.
“Minimum record keeping requirements
Source: Drugs and Cosmetic Act 1940.”

31. 9. Record of blood transfusion reactions:
.Reported transfusion reaction cases should be
investigated.
. Record should be kept.
10. Records of Infectious markers tests:
. Anti-HIV 1& 2Test HBsAg.
. Test Anti-HCV test.
. VDRL/RPR Malaria.
. These all records to be retained for five years.

32. 1. Unique unit identification: 10 years.
2. Medical director approval for exceptions to
donation: 5 years.
3. Donors placed on indefinite deferral: Indefinite
4. Investigation of transfusion-transmitted disease:
10 years.

33. 5. Donor's ABO & Rh type: 5 years.
6. Donor testing for unexplained antibodies:10
years.
7. HCV or HIV look back: 10 years.
8. Recipient's ABO & Rh type: 5 years.

34. 9. Patient's transfusion medical record (unit,
date, vital signs): 10 years.
10. Suspected transfusion complication: 5 years.
11. Archival of obsolete documents: 5 years.
12. Orders for tissue: 10 years.

35. “Testing of random components to ensure
they achieve reliably certain specific
standards • It includes analysis of test
results and detection of irregularities to
identify deficiencies in production of
Blood & Blood Components.”

36. Indian standards
• Drugs and Cosmetics Act 1940, Rules),
Govt of India
• Transfusion Medicine Technical Manual
DGHS, Ministry of Health And Family
Welfare, Govt of India

37. • Blood Bank Standards of NACO, Ministry of
Health and Family Welfare, Govt of India.
• NABH Accreditation Standards for Blood
Banks.

38. 1. REFRIGERATED CENTRIFUGE:
• Buckets & centrifuge bowls – clean with -
warm water and mild detergent - 1% Na
hypochlorite after each spill/breakage
• Calibrated upon receipt, repairs or if low
platelet yields.
• Preventive Maintenance - calibration of
speed with a tachometer (twice a year) -

39. 2. CRYOPRECIPITATE BATH/WATER
BATH:
• Temperature - checked and recorded daily.
• Change water once a week or if leakage
• Recalibration of temperature controller if -
temp. probe/ circuit board replaced - diff. in
digital display & certified thermometer.

40. 3. Platelet Agitator:
• No. of strokes – 60-70/min • Periodic cleaning
& lubrication.
4. Components storage:
. Daily temp check of REFRIGERATORS, FREEZERS
& PLATELET INCUBATOR.
• Visual and audio alarms – check regularly

41. • Monitoring device - THERMOGRAPH (continuous
temperature recorder)
• Temperature check - different locations in large
equipment
• Actual temperature checked with Hg thermometer in
glycerol
• Alarm test - sensor dipped in a beaker with tap
water / ice slush.

42. THANK
YOU………
nityanandu@iul.ac.in