Carbohydrates are generally classified into monosaccharides (simple sugars), oligosaccharides (containing few sugar units) and polysaccharides (containing many sugar units).
Monosaccharides are sugar molecules containing short chain of carbon atoms, one aldehydic or ketonic group and hydroxyl groups attached to remaining Carbon atoms.
Oligosaccharides are formed by polymerisation of monosaccharide molecules by elimination of water molecules.
Polysaccharides are high molecular weight substances composed of large number of moosaccharide units combined to form one large polymer molecule. They may be straight chain or branched chain polymers.
Introduction to Carbohydrates and its ChemistryDHANANJAY PATIL
A Comprehensive Introduction to Carbohydrates its chemistry, classification, qualitative tests an disorders related to its metabolism. This will give readers a overall insight to this topic. All types of queries and suggestions are most welcome
Sugar derivatives and reactions of monosaccharidesNamrata Chhabra
Reactions of monosaccharides, osazone formation, reduction, oxidation, reaction with acids and alkalies, ester formation and formation of amino sugars, amino sugar acids and deoxy sugars.
This ppt explains the properties of monosaccharides, polysaccharides. the properties like mutarotation, reduction, optical activity, caramerlization, osazone is given in the ppt. Also the determination of ring size of the monosaccharide is explained/
A complete review of carbohydrates. definition, source of carbohydrates. Importance, function of carbohydrates. translocation of carbohydrates in plants.
About carbohydrates, its types, physical and chemical properties, isomers and isomeric properties, important carbohydrates, medical use of some carbohydrates.
n chemistry, a glycosidic bond is a type of covalent bond that joins a carbohydrate (sugar) molecule to another group, which may or may not be another carbohydrate.
Carbohydrates are generally classified into monosaccharides (simple sugars), oligosaccharides (containing few sugar units) and polysaccharides (containing many sugar units).
Monosaccharides are sugar molecules containing short chain of carbon atoms, one aldehydic or ketonic group and hydroxyl groups attached to remaining Carbon atoms.
Oligosaccharides are formed by polymerisation of monosaccharide molecules by elimination of water molecules.
Polysaccharides are high molecular weight substances composed of large number of moosaccharide units combined to form one large polymer molecule. They may be straight chain or branched chain polymers.
Introduction to Carbohydrates and its ChemistryDHANANJAY PATIL
A Comprehensive Introduction to Carbohydrates its chemistry, classification, qualitative tests an disorders related to its metabolism. This will give readers a overall insight to this topic. All types of queries and suggestions are most welcome
Sugar derivatives and reactions of monosaccharidesNamrata Chhabra
Reactions of monosaccharides, osazone formation, reduction, oxidation, reaction with acids and alkalies, ester formation and formation of amino sugars, amino sugar acids and deoxy sugars.
This ppt explains the properties of monosaccharides, polysaccharides. the properties like mutarotation, reduction, optical activity, caramerlization, osazone is given in the ppt. Also the determination of ring size of the monosaccharide is explained/
A complete review of carbohydrates. definition, source of carbohydrates. Importance, function of carbohydrates. translocation of carbohydrates in plants.
About carbohydrates, its types, physical and chemical properties, isomers and isomeric properties, important carbohydrates, medical use of some carbohydrates.
n chemistry, a glycosidic bond is a type of covalent bond that joins a carbohydrate (sugar) molecule to another group, which may or may not be another carbohydrate.
ATP synthase—also called FoF1 ATPase is the universal protein that terminates oxidative phosphorylation by synthesizing ATP from ADP and phosphate.
ATP Synthase is one of the most important enzymes found in the mitochondria of cells
Actin filaments, usually in association with myosin, are responsible for many types of cell movements. Myosin is the prototype of a molecular motor—a protein that converts chemical energy in the form of ATP to mechanical energy, thus generating force and movement. The most striking variety of such movement is muscle contraction, which has provided the model for understanding actin-myosin interactions and the motor activity of myosin molecules. However, interactions of actin and myosin are responsible not only for muscle contraction but also for a variety of movements of nonmuscle cells, including cell division, so these interactions play a central role in cell biology. Moreover, the actin cytoskeleton is responsible for the crawling movements of cells across a surface, which appear to be driven directly by actin polymerization as well as actin-myosin interactions.
Carbohydrates - Monosaccharides and its qualitative tests - Part 1Mohamed Mukthar Ali
Discusses about monosaccharides definition, classification, structure and reactions of glucose, galactose, and fructose. Qualitative tests for carbohydrates with reaction scheme. Terminologies in carbohydrates such as epimeris, anomers and mutarotation.
Title: Sense of Smell
Presenter: Dr. Faiza, Assistant Professor of Physiology
Qualifications:
MBBS (Best Graduate, AIMC Lahore)
FCPS Physiology
ICMT, CHPE, DHPE (STMU)
MPH (GC University, Faisalabad)
MBA (Virtual University of Pakistan)
Learning Objectives:
Describe the primary categories of smells and the concept of odor blindness.
Explain the structure and location of the olfactory membrane and mucosa, including the types and roles of cells involved in olfaction.
Describe the pathway and mechanisms of olfactory signal transmission from the olfactory receptors to the brain.
Illustrate the biochemical cascade triggered by odorant binding to olfactory receptors, including the role of G-proteins and second messengers in generating an action potential.
Identify different types of olfactory disorders such as anosmia, hyposmia, hyperosmia, and dysosmia, including their potential causes.
Key Topics:
Olfactory Genes:
3% of the human genome accounts for olfactory genes.
400 genes for odorant receptors.
Olfactory Membrane:
Located in the superior part of the nasal cavity.
Medially: Folds downward along the superior septum.
Laterally: Folds over the superior turbinate and upper surface of the middle turbinate.
Total surface area: 5-10 square centimeters.
Olfactory Mucosa:
Olfactory Cells: Bipolar nerve cells derived from the CNS (100 million), with 4-25 olfactory cilia per cell.
Sustentacular Cells: Produce mucus and maintain ionic and molecular environment.
Basal Cells: Replace worn-out olfactory cells with an average lifespan of 1-2 months.
Bowman’s Gland: Secretes mucus.
Stimulation of Olfactory Cells:
Odorant dissolves in mucus and attaches to receptors on olfactory cilia.
Involves a cascade effect through G-proteins and second messengers, leading to depolarization and action potential generation in the olfactory nerve.
Quality of a Good Odorant:
Small (3-20 Carbon atoms), volatile, water-soluble, and lipid-soluble.
Facilitated by odorant-binding proteins in mucus.
Membrane Potential and Action Potential:
Resting membrane potential: -55mV.
Action potential frequency in the olfactory nerve increases with odorant strength.
Adaptation Towards the Sense of Smell:
Rapid adaptation within the first second, with further slow adaptation.
Psychological adaptation greater than receptor adaptation, involving feedback inhibition from the central nervous system.
Primary Sensations of Smell:
Camphoraceous, Musky, Floral, Pepperminty, Ethereal, Pungent, Putrid.
Odor Detection Threshold:
Examples: Hydrogen sulfide (0.0005 ppm), Methyl-mercaptan (0.002 ppm).
Some toxic substances are odorless at lethal concentrations.
Characteristics of Smell:
Odor blindness for single substances due to lack of appropriate receptor protein.
Behavioral and emotional influences of smell.
Transmission of Olfactory Signals:
From olfactory cells to glomeruli in the olfactory bulb, involving lateral inhibition.
Primitive, less old, and new olfactory systems with different path
Lung Cancer: Artificial Intelligence, Synergetics, Complex System Analysis, S...Oleg Kshivets
RESULTS: Overall life span (LS) was 2252.1±1742.5 days and cumulative 5-year survival (5YS) reached 73.2%, 10 years – 64.8%, 20 years – 42.5%. 513 LCP lived more than 5 years (LS=3124.6±1525.6 days), 148 LCP – more than 10 years (LS=5054.4±1504.1 days).199 LCP died because of LC (LS=562.7±374.5 days). 5YS of LCP after bi/lobectomies was significantly superior in comparison with LCP after pneumonectomies (78.1% vs.63.7%, P=0.00001 by log-rank test). AT significantly improved 5YS (66.3% vs. 34.8%) (P=0.00000 by log-rank test) only for LCP with N1-2. Cox modeling displayed that 5YS of LCP significantly depended on: phase transition (PT) early-invasive LC in terms of synergetics, PT N0—N12, cell ratio factors (ratio between cancer cells- CC and blood cells subpopulations), G1-3, histology, glucose, AT, blood cell circuit, prothrombin index, heparin tolerance, recalcification time (P=0.000-0.038). Neural networks, genetic algorithm selection and bootstrap simulation revealed relationships between 5YS and PT early-invasive LC (rank=1), PT N0—N12 (rank=2), thrombocytes/CC (3), erythrocytes/CC (4), eosinophils/CC (5), healthy cells/CC (6), lymphocytes/CC (7), segmented neutrophils/CC (8), stick neutrophils/CC (9), monocytes/CC (10); leucocytes/CC (11). Correct prediction of 5YS was 100% by neural networks computing (area under ROC curve=1.0; error=0.0).
CONCLUSIONS: 5YS of LCP after radical procedures significantly depended on: 1) PT early-invasive cancer; 2) PT N0--N12; 3) cell ratio factors; 4) blood cell circuit; 5) biochemical factors; 6) hemostasis system; 7) AT; 8) LC characteristics; 9) LC cell dynamics; 10) surgery type: lobectomy/pneumonectomy; 11) anthropometric data. Optimal diagnosis and treatment strategies for LC are: 1) screening and early detection of LC; 2) availability of experienced thoracic surgeons because of complexity of radical procedures; 3) aggressive en block surgery and adequate lymph node dissection for completeness; 4) precise prediction; 5) adjuvant chemoimmunoradiotherapy for LCP with unfavorable prognosis.
Anti ulcer drugs and their Advance pharmacology ||
Anti-ulcer drugs are medications used to prevent and treat ulcers in the stomach and upper part of the small intestine (duodenal ulcers). These ulcers are often caused by an imbalance between stomach acid and the mucosal lining, which protects the stomach lining.
||Scope: Overview of various classes of anti-ulcer drugs, their mechanisms of action, indications, side effects, and clinical considerations.
micro teaching on communication m.sc nursing.pdfAnurag Sharma
Microteaching is a unique model of practice teaching. It is a viable instrument for the. desired change in the teaching behavior or the behavior potential which, in specified types of real. classroom situations, tends to facilitate the achievement of specified types of objectives.
- Video recording of this lecture in English language: https://youtu.be/lK81BzxMqdo
- Video recording of this lecture in Arabic language: https://youtu.be/Ve4P0COk9OI
- Link to download the book free: https://nephrotube.blogspot.com/p/nephrotube-nephrology-books.html
- Link to NephroTube website: www.NephroTube.com
- Link to NephroTube social media accounts: https://nephrotube.blogspot.com/p/join-nephrotube-on-social-media.html
Pulmonary Thromboembolism - etilogy, types, medical- Surgical and nursing man...VarunMahajani
Disruption of blood supply to lung alveoli due to blockage of one or more pulmonary blood vessels is called as Pulmonary thromboembolism. In this presentation we will discuss its causes, types and its management in depth.
Prix Galien International 2024 Forum ProgramLevi Shapiro
June 20, 2024, Prix Galien International and Jerusalem Ethics Forum in ROME. Detailed agenda including panels:
- ADVANCES IN CARDIOLOGY: A NEW PARADIGM IS COMING
- WOMEN’S HEALTH: FERTILITY PRESERVATION
- WHAT’S NEW IN THE TREATMENT OF INFECTIOUS,
ONCOLOGICAL AND INFLAMMATORY SKIN DISEASES?
- ARTIFICIAL INTELLIGENCE AND ETHICS
- GENE THERAPY
- BEYOND BORDERS: GLOBAL INITIATIVES FOR DEMOCRATIZING LIFE SCIENCE TECHNOLOGIES AND PROMOTING ACCESS TO HEALTHCARE
- ETHICAL CHALLENGES IN LIFE SCIENCES
- Prix Galien International Awards Ceremony
Explore natural remedies for syphilis treatment in Singapore. Discover alternative therapies, herbal remedies, and lifestyle changes that may complement conventional treatments. Learn about holistic approaches to managing syphilis symptoms and supporting overall health.
Flu Vaccine Alert in Bangalore Karnatakaaddon Scans
As flu season approaches, health officials in Bangalore, Karnataka, are urging residents to get their flu vaccinations. The seasonal flu, while common, can lead to severe health complications, particularly for vulnerable populations such as young children, the elderly, and those with underlying health conditions.
Dr. Vidisha Kumari, a leading epidemiologist in Bangalore, emphasizes the importance of getting vaccinated. "The flu vaccine is our best defense against the influenza virus. It not only protects individuals but also helps prevent the spread of the virus in our communities," he says.
This year, the flu season is expected to coincide with a potential increase in other respiratory illnesses. The Karnataka Health Department has launched an awareness campaign highlighting the significance of flu vaccinations. They have set up multiple vaccination centers across Bangalore, making it convenient for residents to receive their shots.
To encourage widespread vaccination, the government is also collaborating with local schools, workplaces, and community centers to facilitate vaccination drives. Special attention is being given to ensuring that the vaccine is accessible to all, including marginalized communities who may have limited access to healthcare.
Residents are reminded that the flu vaccine is safe and effective. Common side effects are mild and may include soreness at the injection site, mild fever, or muscle aches. These side effects are generally short-lived and far less severe than the flu itself.
Healthcare providers are also stressing the importance of continuing COVID-19 precautions. Wearing masks, practicing good hand hygiene, and maintaining social distancing are still crucial, especially in crowded places.
Protect yourself and your loved ones by getting vaccinated. Together, we can help keep Bangalore healthy and safe this flu season. For more information on vaccination centers and schedules, residents can visit the Karnataka Health Department’s official website or follow their social media pages.
Stay informed, stay safe, and get your flu shot today!
Report Back from SGO 2024: What’s the Latest in Cervical Cancer?bkling
Are you curious about what’s new in cervical cancer research or unsure what the findings mean? Join Dr. Emily Ko, a gynecologic oncologist at Penn Medicine, to learn about the latest updates from the Society of Gynecologic Oncology (SGO) 2024 Annual Meeting on Women’s Cancer. Dr. Ko will discuss what the research presented at the conference means for you and answer your questions about the new developments.
3. Each year, 100 metric tons of CO2 is converted to Carbohydrates by plants
4. Carbohydrates
• Carbohydrates are the most abundant organic
molecules in nature.
• Hydrates of carbon
• Carbohydrates are defined as polyhydroxyaldehydes
or ketones or compounds which produce them on
hydrolysis.
• Composed of carbon, hydrogen and oxygen
5. • General molecular formula Cn (H2O)n
• Some carbohydrates contain Sulphur, Nitrogen or
Phosphorus
• Exceptions are acetic acid C2H4O2 and lactic acid
C3H6O3.
6. Functions of Carbohydrates
• Main sources of ENERGY in body (4kcal/g)
– RBCs and Brain cells have an absolute requirement of
carbohydrates.
• Storage form of energy (starch and glycogen)
• Excess carbohydrate is converted to fat.
• Glycoproteins and glycolipids are components of cell
membranes and receptors.
• Structural basis of many organisms.e.g. Cellulose in plants,
exoskeleton of insects, cell wall of microbes,
mucopolysaccharides and ground substance in higher
organisms.
7. • Oligosaccharide Chains Encode Biologic Information
• An enormous number of glycosidic linkages can be
generated between sugars.
• For example, three different hexoses may be linked to
each other to form over 1000 different trisaccharides.
• Oligosaccharide chains encode biologic information
and this depends upon their constituent sugars, their
sequences, and their linkages.
8. • The biologic information that sugars contain is
expressed via interactions between specific sugars,
either free or in glycoconjugates, and proteins (such
as lectins) or other molecules.
• These interactions lead to changes of cellular
activity.
9. • Thus, deciphering the so-called ‘sugar code of life’
(one of the principal aims of glycomics) entails
elucidating all of the interactions that sugars and
sugar-containing molecules participate in, and also
the results of these interactions on cellular behavior.
• This will not be an easy task, considering the diversity
of glycans found in cells.
10. Carbohydrates
Disaccharides
2 sugar units
Oligosaccharides
3-9 units
Polysaccharides
>10
Homoglycans Heteroglycans
e.g.Glucose,
fructose etc
e.g.Sucrose e.g. Maltotriose
e.g. starch,
glycogen
e.g. GAGs or
glycosaminoglycans
11. Monosaccharides
• Molecules having only one actual or potential sugar group
No. of
carbon
Generic name
Aldoses Ketoses
3 Trioses
Aldotriose e.g. glyceraldehyde Ketotriose e.g. Dihydroxyacetone
4 Tetroses
Aldotetrose e.g.
Erythrose
Ketotetrose e.g.
Erythrulose
5 Pentoses
Aldopentoses e.g
Arabinose, Xylose, Ribose
Ketopentoses e.g.
Xylulose, Ribulose
6 Hexoses
Aldohexose e.g. Glucose,
Galactose, Mannose Ketohexose e.g. Fructose
7 Heptoses
Aldoheptose: Glucoheptose Ketoheptose e.g Sedoheptulose
14. Stereoisomers
• Compounds having same structural formula, but
differing in spatial configuration as known as
stereoisomers.
• Asymmetric carbon:
• Four different groups are attached to the same
carbon.
• The reference molecule is glyceraldehyde.
• All monosaccharides can be considered as molecules
derived from glyceraldehyde by successive addition of
carbon atoms.
16. D and L Isomers of glucose
• D and L Isomers are mirror images of each other.
• The spatial orientation of H & OH groups on the C-atom
(C5 for glucose), adjacent to the terminal
primary alcohol carbon determines whether the
sugar is D or L Isomer.
• If the OH group is on the right side, the sugar is of D-Isomer.
• If the OH group is on the left side, the sugar is of L-Isomer.
• Mammalian tissues have D- sugars.
21. Optical activity
• Optical activity is a characteristic feature of compounds with
asymmetric carbon atom.
• When a beam of polarized light is passes through a solution of
an optical isomer, it will be rotated either to the right or left.
• Depending on the rotation, molecules are called
dextrorotatory (+) or levorotatory (-).
• Racemic mixture: If D & L isomers are present in equal
concentration, it is known as racemic mixture.
• NOTE: Racemic mixture does not exhibit any optical activity.
22. Epimers
• If two monosaccharides differ from each other in their
configuration around a single specific carbon atom, they are
referred as epimers to each other.
• Glucose & galactose are C4-epimers
• Glucose & mannose are C2-epimers
• Inter-conversion of epimers is known as epimerization,
epimerases catalyzes this reaction.
23. Enantiomers
• Enantiomers are a special type of stereoisomers, that
are mirror images of each other.
• Majority of sugars in higher animals are of D-type.
24. Diastereoisomers
• The term diastereomers is used to represent the stereoisomers that
are not mirror images of one another.
• Configurational changes with regard to C2 , C3 and C4 will produce
eight different monosaccharides.
Total D + L forms = 16 isomers of glucose
25. • Reference Carbon atom of Sugars:-
• Penultimate carbon atom is the reference carbon atom for
naming sugars.
• All monosaccharides can be considered as molecules derived
from glyceraldehyde by successive addition of C units.
Penultimate
carbon
26. Glycosides
• When the hemi-acetal group (hydroxyl group of
the anomeric carbon) of a monosaccharide
iscondensed with an alcohol or phenol group, it is
called a glycoside.
• The non-carbohydrate group is called aglycone.
28. Between
C1 -CHO and C5 -OH
The common
monosaccharides have
cyclic structures
29. Anomerism
• Anomers have same composition but differ in the orientation of groups
around anomeric carbon atom.
• Anomeric carbon is a carbonyl carbon atom, e.g. 1st carbon atom in
glucose is anomeric carbon atom.
• Carbonyl carbon atom becomes asymetric because of ring structures of
monosaccharides in solution thus anomers are encountered in cyclic
structures of monosaccaharides.
• The alpha & beta cyclic forms of D-glucose are known as anomers.
• They differ from each other in the configuration only around C1 known as
anomeric carbon.
• The hemiacetal (or carbonyl) carbon atom is called the anomeric carbon.
• In case of alpha anomer, the OH group held by anomeric carbon is on the
opposite side of the group CH2OH of sugar ring.
30. Expression
• Anomers are expressed as α and β forms.
• In α form “OH” group is below the plane (OH
group is oriented away from the oxygen atom)
• In β form “OH” group is above the plane (OH
group is oriented towards the oxygen atom)
31. • Mutation:
• When D-glucose is crystallized at room temperature and a fresh
solution is prepared, its specific rotation of polarized light is
112o; but after 12- 18 hrs it changes to +52.5 o
• This change in rotation with time is called as mutarotation.
• Glucose has two anomers α and β.
Traces of linear forms,
intermediate forms
35. Reactions of monosaccharides
• Tautomerization:
• The process of shifting a hydrogen atom from one carbon atom to
another to produce enediols is known as tautomerization.
• Reducing properties:
• In mild alkaline solutions, carbohydrates containing a free sugar
group (aldehyde or ketone) will tautomerise to form enediols ,
where two hydroxyl groups are attached to the double-bonded
carbon atoms.
• Since enediols are powerful reducing agents in alkaline medium.
• When oxidizing agents like cupric ions are present , sugars form a
mixture of carboxylic acids by breaking at the double bonds.
36. Benedict’s test
• Procedure: 0.5 (8 drops) ml urine + 5ml Benedict’s reagent & boil for 2
mins.
• Interpretation:
• Drawback – test is not specific for glucose
• Fehling’s test: No intermediate colors are formed as because over there
a powerful reducing agent KOH is used.
Observation Inference
No change in colour No sugar
Green colour 0-0.5mg% +
Yellow 0.5-1.0mg% ++
Orange 1.0-1.5mg% +++
Brick red 1.5-2mg% ++++
37. Osazone formation
• All reducing sugars will form osazones with excess of phenylhydrazine when
kept at boiling temperature.
• Osazones are insoluble.
• Osazones of individual sugars have characteristic crystal from
• The differences in glucose, fructose and mannose are dependent on 1st and 2nd
C & this difference is masked when Phenyhydrazine reacts with these two
carbons.
• So, Glucose, Fructose and Mannose give broom shaped osazones.
39. Oxidation of Sugars
1) Mild Oxidation Conditions: e.g. hypobromous
acid, the aldehyde group is oxidized to
carboxyl group to produce ALDONIC acids.
Glucose Gluconic acid
Mannose Mannonic acid
Galactose Galactonic acid
2) When Aldehyde group is protected, and the
molecule is oxidized, esp. in the body, the last
C is oxidized to COOH producing URONIC
acids.
Imp- Glucuronic acid is used in
body to synthesize heteropolysaccharides and
also for conjugation of various substances.
40. • Under strong oxidation conditions(e.g strong acids-HNO3)
• BOTH groups are oxidized to produce dicarboxylic acids called
acids called SACCHARIC acids
Glucose --> Glucosaccharic acid
Mannose --> Mannaric acid
Galactose --> Mucic acid
• Oxidation by glucose oxidase:- GOD-POD method
41. Furfural formation
• Monosaccharides when treated with concentrated H2SO4 undergoes
dehydration with the removal of 3 molecules of water.
• Hexoses give hydroxymethyl furfural and pentoses give furfural.
• Furfurals condense with phenolic compounds to give various colors.
E.g. Molisch’s test: General test for carbohydrates (H2SO4 and α-naphthol)
• Rapid Furfural and Seliwanoff’s test: Tests for presence of keto group
42. Reduction to form alcohols
• When treated with reducing agents such as sodium amalgam,
hydrogen can reduce sugars.
• Aldose yields corresponding alcohol.
• Ketoses form two alcohols because of appearance of new
asymmetric carbon in this process.
D-Glucose D-Sorbitol D-Fructose D-Mannitol
• Sorbitol and Mannitol are used to identify bacterial colonies.
• Mannitol is used to reduce intracranial pressure by forced
diuresis.
• The osmotic effect of sorbitol and dulcitol produces changes in
tissues when they accumulate in abnormal amounts. E.g cataract
43. Glycosides
• When the hemi-acetal group (hydroxyl group of the anomeric
C ) of a monosaccharide is condensed with an alcohol or
phenol group, it is called as a glycoside.
• The non-carbohydrate group is called aglycone.
• Glycosides are non –reducing (WHY ?) but they may be
hydrolyzed by boiling with dilute acids.
• - glycosides are hydrolyzed by maltase from yeast, while
beta-glycosides are hydrolyzed by Emulsin from almonds.
• So enzyme hydrolysis affords a method to distinguish b/w two
forms.
44.
45. Important Glycosides
Sugar Aglycon Glycoside Source Importance
Glucose Phloretin Phlorizin Rose bark Renal damage
Galactose
Xylose
Digitogenin Digitonin Leaves of
foxglove
Cardiac
stimulant
Glucose Indoxyl Plant
indican
Leaves of
indigofera Stain
46. Formation of esters
• Hydroxyl groups of sugars can be esterified to form
acetates, propionates, benzoates, etc
• Sugar phosphates are of great biological importance.
• Metabolism of sugars inside the body starts with
phosphorylation.
e.g Glucose 6-P04
47. Amino sugars
• Amino groups may be substituted for hydroxyl groups of sugars to
give rise to amino sugar.
• Generally the group is added to the second C of hexoses.
• They are non –reducing and do not form osazones
• They are found in GAGS, glycoproteins, proteoglycans
• Abbreviations:-
GluNac = N-acetyl –glucosamine
GalNac =N-acetyl-galactosamine
GLUCOSAMINE or 2 amino-D-glucopyranose
(α form)
48. • The amino group may be further acetylated to
produce N-acetlyated sugars like N-acetly glucosamine
(GlcNac) or N-acetyl galactosamine (GalNac) which are
important constituents of glycoproteins and MPS
49. Deoxy Sugars
• Oxygen of the hydroxyl group may be removed to form deoxy
sugars.
• They are non reducing.
• Don’t form osazones.
• Deoxyribose is present in DNA
50. Disaccharides
• When two monosaccharides are combined together by
glycosidic linkage, a disaccharide is formed.
• Two types:-
Non-reducing
Sucrose Cane sugar
Trehalose in yeast
Reducing
Lactose Milk sugar
Maltose Malt sugar
52. Inversion
• Hydrolysis of sucrose (optical rotation +66.5o) will
produce one molecule of glucose (+52.5o) and one
molecule of fructose (-92o)
• Therefore the products will change the dextrorotation
to levorotation (INVERSION)
• Equimolecular mixture of glucose and fructose thus
formed is called as Invert Sugar
• The enzyme producing hydrolysis of sucrose is called
INVERTASE
55. Isomaltose
• 2 Glucose in (16) linkage
• Reducing disaccharide
• Produced during partial hydrolysis of starch and glycogen
56. Disaccharides of importance
Sugar Composition Source Clinical Significance
Sucrose
D-glucopyranosyl-
(1-2) D-fructo
furanoside
Cane and beet sugar,
sorghum and some
fruits and vegetables
Rare genetic lack of sucrase
leads to sucrose intolerance—
diarrhea and flatulence
Lactose
D-galactopyranosyl-
(1-4)D-glucopyranose
Milk
Lack of lactase leads to lactose
intolerance diarrhea and
flatulence; may be excreted in
the urine in pregnancy
57. Sugar Composition Source Clinical Significance
Maltose
D-glucopyranosyl-
(1-4)-D-glucopyranose
Enzymatic hydrolysis of starch
(amylase); germinating cereals
and malt
Isomaltose
D-glucopyranosyl-
(1-6)-D-glucopyranose
Enzymatic hydrolysis of starch
(the branch points in
amylopectin)
Lactulose
D-galactopyranosyl-
(1-4)-D-fructofuranose
Heated milk (small amounts),
mainly synthetic
Not hydrolyzed by
intestinal enzymes, but
fermented by intestinal
bacteria; used as a mild
osmotic laxative
Trehalose D-glucopyranosyl-
(1-1)-D-glucopyranoside
Yeasts and fungi; the main sugar
of insect hemolymph
61. Starch
• Carbohydrates of the plant kingdom
• Sources:
• Potatoes, tapioca, cereals (rice, wheat) and
other food grains
• Composed of Amylose & Amylopectin
• Amylose:
• When starch is treated with boiling water, 10 -20
% is solubilized.
• This part is called amylose, contains glucose
units with -1,4 glycosidic linkages.
• Mol wt =400,000 or more
62. • Amylopectin:
• The insoluble part absorbs water and forms paste
like gel;
• This is called as amylopectin.
• Amylopectin is also made up of glucose units, but is
highly branched with molecular weight more than 1
million.
• The branching points are made by - 1, 6 linkage
63.
64. Hydrolysis of starch
• Starch will form a blue coloured complex with iodine; this
color disappears on heating and reappears when cooled.
• This is a sensitive test for starch.
• When starch is hydrolyzed by mild acid, smaller and
smaller fragments are produced.
• The hydrolysis for a short time produces amylodextrin
(violet color with iodine and non-reducing).
• Further hydrolysis…………….
amylodex erythrodexarchrodextrinMaltose
Violet Red no color no color
Non reducing Non reducing Reducing Reducing
65. Action of amylases on starch
• Salivary amylases and pancreatic amylases are
amylases, which act randomly on , 1-4
linkages to split starch into smaller units
called dextrins
• Beta amylases (plant origin – almonds etc) act
consecutively from one end.
• When beta amylases reach a branch point in
amylopectin, enzyme is blocked, leaving a large
molecule called as LIMIT DEXTRIN
66. Glycogen
• Storage form of energy in animal.
• Stored in liver and muscle
• Stores more glucose residues per gram than starch.
• More branched and compact than starch.
• Less osmotic pressure.
• More energy in a smaller space.
• Glycogen in liver (6-8%) is higher than that in the
muscles (1-2%).
• Liver glycogen - first line of defense against declining
blood glucose levels especially between meals.
67. • A homopolysaccharide: linear chain of (1→4) linked glucosyl
residues with branches joined by (1→6) linkages
68. Cellulose
• Glucose units combined by -1,4 linkages.
• Straight line str. with no branches.
• Mol wt 2-5 million.
• This bond is digested by cellobiases an enzyme not
present in humans.
• Herbivores animals have large caecum which harbor
bacteria which break cellulose.
• White ants (termites) and some wood fungi also have
cellulase.
• Commercial applications: nitrocellulose, cellulose
acetate membranes for electrophoresis ETC
69. Inulin
• D -fructose in -1,2 linkages.
• Source:
• Bulbs and tubers chicory, dahlia, dandelion,
onions, garlic.
• Not metabolized .
• Not absorbed nor secreted by kidneys.
• USE – to measure GFR.
70. Dextrans
• Highly branched homoglycan containing Glu
residues in 1-6, 1-4 and 1-3 linkages.
• Produced by microbes.
• Mol. wt:- 1-4 million.
• As large sized, they will not move out of vascular
compartment so used as plasma expanders.
71. Chitin
• N-acetyl glucosamine with beta 1,4 glycosidic
linkage
• Exoskeleton of crustacea and insects.
73. Agar and Agarose
• It is made up of D-galactose and an L-galactose derivative
ether – linked between C-3 and C-6
• It is dissolved in water at 100 o C, which upon cooling sets into
a gel.
• Agar cannot be digested by bacteria so it is widely used as a
supporting media to culture bacterial colonies.
• Also used as a supporting agent for immuno-diffusion and
immuno-electrophoresis.
• Two components : Agarose (unbranched)
Agaropectin (branched)
• Agarose is made up of D- galactose combined with 3,6-
anhydro L-galactose units and is used as a matrix for
electrophoresis.
74. Mucopolysaccharides or GAG
[ URONIC ACID + AMINO SUGAR] n
Acetylated amino sugars, sulfate and carboxyl groups
may be present also
75. Heteropolysaccharides
• Polymers made from more than one kind of
monosaccharides or monosaccharide derivatives.
• Eg : Glycosoaminoglycans,
Agar
Agarose
76. • Long, Unbranched heteropolysaccharide, made of
repeating disaccharide units containing uronic acid &
amino sugars.
• Amino sugar – Glucosamine or Galactosamine
(Present in there acetylated form)
• Uronic acid – D-Glucuronic acid or L-Iduronic acid
• GAGs are the most important group of heteroglycan in
humans.
77. • First isolated from mucin so called
mucopolysaccharides.
• Major components of extracellular matrix of
connective tissue, including bone and cartilage,
synovial fluid, vitreous humor and secretions of
mucus producing cells.
78. • Gel forming component of extracellular matrix
• The anionic groups (carboxy & sulfate groups) being strongly
hydrophilic tend to bind large amount of water producing gel
like matrix, that forms the bodies ground substance.
• Heteropolysaccharide chains repel one another and therefore
exist in extended conformation in solutions.
• This produces slippery consistency of mucus secretions and
synovial fluid.
79. • Structural support to connective tissue
• GAGs form matrix or ground substance that stabilizes and
supports the cellular and fibrous components of tissues.
• Other functions:
• Plays an important role in mediating cell-cell interactions
• Their slippery consistency makes them suitable for a
lubricant action in joints.
81. Hyaluronic acid
• It is sulfate free GAG.
• Synovial fluid of joints, vitreous humor,
connective tissues and cartilage.
82. Functions of Hyaluronic acid
• Serves as a lubricant and shock absorbant in joints.
• Acts as seives in extracellular matrix.
• Permits cell migration during morphogenesis &
wound repair.
• Hyaluronidase is an enzyme that breaks β1 – 4
linkages of hyaluronic acid.
• Present in high concentration in seminal fluid, & in
certain snake and insect venoms.
83. • Hyaluronidase enzyme of semen degrades the gel
around ovum & allows effective penetration of
sperm into ovum, thus helps in fertilization.
• The invasive power of some pathogenic organism
may be increased because they secrete
hyaluronidase.
85. • Widely distributed in bone, cartilage & tendons.
• Functions:
• In cartilage, it binds collagen & hold fibers in a tight
strong network.
• Role in Compressibility of cartilage in weight bearing.
86. Dermatan sulfate
• Contains repeating units of L-iduronic acid and N-acetyl
glucosamine 4 sulfate.
87. • Present in skin, cardiac valves & tendon.
• Function:
• Present in sclera of eye where it has important
function in maintaining overall shape of eye.
88. Heparin
• Only GAG present intracellular: In granules of mast
cells and also in lung, liver and skin.
89. • Strongly acidic due to presence of more sulfate group.
• Functions:
• It is an anticoagulant (prevents blood clotting)
• Heparin helps in the release of the enzyme lipoprotein
lipase (LPL) which helps to clear the lipidemia after
fatty meal – so called clearing factor.
90. Heparan sulfate
• Structurally similar to heparin, but has a
• Lower molecular weight
• Contains higher acetyl groups & less sulfate group
• Predominant uronic acid is D-Glucuronic acid
• It is an extracellular GAG found in basement membrane and is
an essential component of cell surfaces.
• Determines charge selectiveness of renal glomerulus.
91. Keratan sulfate
• Only GAG with no uronic acid.
• Found in cornea & tendons.
• Function:
• Maintains the corneal transparency.
94. Blood group substances (blood gr Antigens)
• RBC membrane contains several antigenic substance,
based on which classified into different blood groups.
• They contain carbohydrates as glycoproteins or
glycolipids.
• N-Acetylgalactosamine, galactose, fucose, sialic acid
etc are found in blood gr substances.
• Carbohydrate content plays a determinant role in
blood grouping.
95. Agar
• Contains galactose, glucose & other sugars.
• Obtained from sea weeds
• Functions:
• Cannot be digested by bacteria.
• So used as supporting agent to culture bacterial
colonies.
• Also as support medium of immuno diffusion &
immuno-electrophoresis.
96. Agarose
• Galactose and 3,6 anhydrous galactose units
• Used as matrix for electrophoresis.
97.
98. Reference Books
• Test Book of Biochemistry- Harper
• Test Book of Biochemistry - Dr. U.Satyanarayana
• Test Book of Medical Biochemistry-DM.Vasudevan
• Test Book of Medical Biochemistry – MN Chatterjea