Metabolism of amino acids (general metabolism).
Part - I of amino acid metabolism.
This presentation covers Transamination, deamination, formation and Transport of Ammoniaand etc.
Are most abundantly distributed organic compounds.
70 kg man= protein weight constitute 12 kg
Skeleton and connective tissue contains half
Body protein and other half is intracellular.
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Are most abundantly distributed organic compounds.
70 kg man= protein weight constitute 12 kg
Skeleton and connective tissue contains half
Body protein and other half is intracellular.
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Formation and fate of Ammonia
Transdeamination, oxidative and non oxidative deamination, Ammonia transport, Ammonia intoxication, Ammonia detoxification
Fate of Glucogenic and Ketogenic amino acid
Amino acid are the currency of of nitrogen and protein economy of the host, hence they are used in many pathways beyond protein synthesis, including energy production and neurotransmitter synthesis.
All amino acid are comprised of an amino group and a carbon skeleton. During metabolism these two parts are separated as they have different ‘fates’
Of the liberated amino acid approximately 75% are utilized while remainder serve as precursors for important biological compound and those not utilized are degraded to amphibolic intermediates
The pathway of amino acid catabolism is quite similar in most organism
Amino acids function as monomers of polypeptides.
Energy metabolites.
Precursors for nitrogen-containing compounds (heme, glutathione, nucleotides, coenzymes)
Amino acids are classified into 2 groups: essential and nonessential
Mammals can synthesize nonessential amino acids from metabolic precursors.
Essential amino acids must be taken in from diet.
Excess dietary amino acids are converted to common metabolic intermediates: pyruvate, OAA, acetyl-CoA, and -ketoglutarate.
Fates of Amino Acids
🠶 Amino Acid Utilization
🠶 Amino-group metabolism
🠶 Explain role of transamination reactions in aa synthesis and identify vitamin essential for this reaction (tie in to urea cycle)
🠶 Describe interconversion between ketoacids and AA, including requirement of
pyridoxal phosphate (PLP) as a cofactor
🠶 Outline formation and transport of ammonia
🠶 Describe importance of reactions catalyzed by glutamine synthetase, glutaminase, and glutamate dehydrogenase
🠶 Ammonia Intoxication
🠶List causes for hyperammonemia, its consequences, and treatments to reduce blood ammonia levels
Overview of amino acid anabolism and catabolism and fate of ammonia in amino acid metabolism. This is targeted for MBBS, MD, BDS and general Biochemistry students
Amino acid oxidation and the production of urea,
Catabolic pathways for phenylalanine and tyrosine.
Summary of the glucogeneic and ketogenic amino acids.
disorders of amino acids
Similar to Metabolism of amino acids (general metabolism) (20)
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3. 8
Proteins are nitrogen-containing macromolecules consisting
of L-α-amino acids
AA catabolism is part of the whole body catabolism
Nitrogen enters the body in a variety of compounds present
in the food, the most important being AAs present in the
dietary protein.
Nitrogen leaves the body as urea, ammonia, and other
products derived from AA metabolism.
5. 10
Degradation
• 300 to 400 gm/day
Synthesis
• 300 to 400 gm/day
The turnover is high in infancy and
decreases with age advance.
6. 11
Control of protein turnover
• The turnover of the protein is
influenced by many factors.
• A small polypeptide called
ubiquitin tags with the proteins
and facilitates degradation.
7. 12
NITROGEN BALANCE
Catabolism of amino acids leads to a net loss of nitrogen
from the body.
This loss must be compensated by the diet in order to
maintain a constant amount of body protein.
Nitrogen balance studies evaluate the relationship
between the nitrogen intake and nitrogen excretion.
8. 13
NITROGEN BALANCE
Three situations of nitrogen balance are possible as follows:
1.Nitrogen equilibrium
• In normal adults, nitrogen intake = nitrogen excretion.
• the rate of body protein synthesis = the rate of degradation
1.Positive nitrogen balance
• nitrogen intake > nitrogen excretion
• It shows that nitrogen is retained in the body
• This occurs in growing infants and pregnant women.
Negative nitrogen balance
• nitrogen intake < nitrogen excretion
• this occurs during serious illness & major injury & trauma, in advanced cancer
• If the situation is prolonged, it will ultimately lead to death
11. 16
Fate of alpha amino group of amino acids
o The presence of the α- amino group keeps amino acids safely locked away
from oxidative breakdown.
o Removal of α- amino group is an obligatory step in the catabolism of all
amino acids
o Once removed this nitrogen can be incorporated in to other compounds or
excreted, with the carbon skeleton being metabolized
o Different animals excrete excess nitrogen as ammonia, uric acid, or urea.
14. 19
• The transfer of an amino (-NH2) group from an amino acid to a keto
acid, with the formation of a new A. A & a new ketoacid.
• Catalysed by a group of enzymes called transaminases
(aminotransferases)
17. 22
• Pyridoxal Phosphate – COFACTOR
• Transamination is reversible.
• It involves both anabolism and catabolism,
• It diverts the excess AAs towards energy production.
• There is no free NH3 liberated, only transfer of amino group.
• Except lysine, threonine, proline, and hydroxy proline, all AAs participate
in transamination reactions.
• Transamination is not restricted to α -amino groups.
• The δ -amino group of ornithine and the Ʃ -amino group of lysine—readily
undergoes transamination.
• Liver, Kidney, Heart, Brain- adequate amount of these enzymes.
Characteristics of
Transamination
18. 23
• This reaction provides a mechanism for collecting the amino groups
from various AAs into one common product L-glutamate.
• This is important because glutamate is the only AA whose α-amino
group can be directly removed at a high rate by oxidative
deamination.
• Functions both in amino acid catabolism & biosynthesis.
• The synthesis of non-essential amino acids.
Metabolic significance of Transamination reactions
Clinical significance of transaminase enzyme
Serum levels of some transaminases are elevated in some disease state and
measurement of these are useful in medical diagnosis, e.g. ALT (SGPT) and AST
(SGOT) are important in the diagnosis of liver and heart damage
19. 24
Role of B6 Phosphate in Transamination
• PLP, a derivative of vitamin B6
• The coenzyme pyridoxal phosphate (PLP) is
present at the catalytic site of aminotransferases and of
many other enzymes that act on amino acids.
• During transamination, bound PLP serves as a carrier of
amino groups.
• Rearrangement forms an α-keto acid and enzyme-bound
Pyridoxamine phosphate, which forms a Schiff base with a
second keto acid.
21. 26
Role of B6 Phosphate in transamination
• The transfer of α-amino group from donor amino acid to Pyridoxal
phosphate forms Pyridoxamine phosphate, and a keto acid.
• The α-amino group is finally passed on to acceptor α-keto acid to form a
new amino acid.
22. 27
Aspartate transaminase (AST / SGOT)
– mitochondrial enzyme.
- heart, liver, muscle, kidney.
- specific to heart damage
- important in liver since half of urea-N is from Aspartate.
23. 28
– cytosolic enzyme,
- specific to liver damage.
- important in muscle where ~25% of AA-N is transported out as
alanine
- In liver, reverse reaction moves AA-N back on GLU .
Alanine Transaminase (ALT/ SGPT)
26. 31
Deamination
The removal of amino group from A.A , as free NH3.
This occurs both as
Oxidative deamination.
Non-oxidative deamination.
27. 32
In this deamination coupled with oxidation.
Removal of NH3 from amino group of Glutamate, coupled with
oxidation.
Glutamate is the only AA , which undergoes oxidative deamination at a
significant rate.
Site:
Liver and kidney.
Mitochondria.
Catalysed by Glutamate Dehydrogenase –
Co-enzyme – NAD+ / NADP + .
28. 33
Glutamate dehydrogenase (gdh)
Mitochondrial enzyme
Metalloenzyme (Zn 2+).
It is the only enzyme that can accept either NAD+ or
NADP + as its coenzyme.
31. 36
Regulation of GDH activity
• The activity of glutamate dehydrogenase is allosterically
regulated.
• Consists of 6 identical subunits .
• Allosteric regulation-
• GTP and ATP – allosteric inhibitors.
• GDP and ADP - allosteric activators.
• ↓ Energy - ↑ oxidation of A.A.
• Steroid and thyroid hormones inhibit GDH
32. 37
Metabolic Significance
• Reversible Reaction
• Both Anabolic and Catabolic.
• Catabolic – Channels nitrogen from Glutamate to urea.
• Anabolic – amination of α- KG by NH3 to Glutamate.
33. 38
Role of Glutamate
• Glutamate occupies a central place in the amino acid metabolism.
• Basically it acts as a collector of amino group of the amino acids.
• All the amino nitrogen from amino acids that undergo transamination
can be concentrated in glutamate.
• L-glutamate is the only amino acid that undergoes oxidative
deamination at an appreciable rate in mammalian tissues.
• The formation of ammonia from α -amino groups thus occurs mainly
via the α -amino nitrogen of L-glutamate.
34. 39
Role of Glutamate & Glutamate dehydrogenase
• Since in majority of the transamination reactions alpha ketoglutarate
is the acceptor keto acid forming Glutamate, that is oxidatively
deaminated in the liver by Glutamate dehydrogenase to form alpha
ketoglutarate and ammonia.
• Conversion of α-amino nitrogen to ammonia by the concerted action
of glutamate aminotransferase and GDH is often termed
"transdeamination."
• Thus Transamination and deamination are coupled processes though
they occur at distant places.
37. 42
Amino acid Oxidases
L-amino acid oxidase and D-amino acid oxidase
Flavoproteins
Cofactors - FMN and FAD .
Site - Liver, kidney .
Peroxisomes .
Activity of L-AA Oxidase is low.
Thus , a minor role in AA catabolism.
38. 43
L-amino acid α- keto acid + NH3
L-amino acid oxidase
FMN FMNH2
H2O2 ½ O2
Catalase
H2O
L-AA Oxidase – acts on all A.A, except Hydroxy , dicarboxylic AA .
39. 44
D-amino acid α- keto acid + NH3
D-amino acid oxidase
FAD FADH2
H2O2 ½ O2
Catalase
H2O
Activity of D-AA oxidase is high than that of L-AA oxidase.
(Degrades D-AA in bacterial cell wall ).
40. 45
Metabolic fate of D - amino acids
D-amino acid
α- keto acid
D-amino acid oxidase
FAD
FADH2
H2O
NH4
+
L – amino acid Glucose, Fats
Energy
41. 46
Fate of D-amino acids
• D-amino acids are found in plants and microorganisms.
• They are not present in mammalian proteins
• D-amino acids are taken in the diet / bacterial cell wall, absorbed
from gut - acted on by D- AA oxidase to the respective α-keto acids.
• The α-keto acids undergo transamination to be converted to L-amino
acids Which participate in various metabolic pathways.
42. 47
Non -Oxidative deamination
• Direct deamination, without oxidation.
Amino acid Dehydratases :
• Serine, threonine and homoserine are the hydroxy amino acids.
• They undergo non-oxidative deamination catalyzed by PLP-
dependent dehydratases
PL
P
Serine
Threonine
Homoserine
Respective Ketoacid
Dehydratase
NH3
43. 48
HO CH2
H
C COO
NH3
+
C COO
OH2O NH4
+
C COO
NH3
+
H2C H3C
H2O
serine aminoacrylate pyruvate
Serine Dehydratase
44. 49
Amino acid desulfhydrases
• Cysteine and homocysteine undergo deamination coupled with
desulfhydration to give keto acids.
•
• Deamination of histidine:
Cysteine Pyruvate
Desulfhydrases
NH3 +H2S
Histidine Uroconate
Histidase
NH3
46. 51
At the physiological pH, ammonia exists as NH4
+ ions.
Formation of ammonia:
Amino acids – by Trans-deamination.
Biogenic amines
Pyrimidine catabolism.
by action of intestinal bacteria on urea.
metabolism of ammonia
47. 52
Ammonia is produced in most tissues.
Since ammonia is extremely toxic,
For the ultimate conversion of ammonia to urea, it is transported
to the liver.
It is immediately converted to nontoxic metabolites such as,
glutamate (All tissues )
glutamine (Brain)
alanine (Muscle) &
ultimately to urea.
metabolism of ammonia
48. 53
Since free ammonia is highly toxic, it is never transported in free form
in blood.
Two mechanisms are available in humans for the transport of
ammonia from the peripheral tissues to the liver for its ultimate
conversion to urea.
Transport of Ammonia in the Form of Glutamine
Transport of Ammonia in the Form of Alanine
Transport of ammonia
49. 54
Glutamine is a storehouse of NH3.
It is present at the highest conc. in blood among the AAs.
Glutamine serves as a storage and transport form of NH3.
In many tissues (liver, kidney and brain), ammonia is enzymatically
combined with glutamate to yield glutamine by the action of
glutamine synthetase.
Transport of Ammonia in the Form of Glutamine
50. 55
The glutamine, so formed is a neutral nontoxic major transport form
of ammonia.
The glutamine is transported by blood to the liver, where it is cleaved
by glutaminase to yield glutamate and free ammonia.
The ammonia so formed is converted by the liver into urea.
Transport of Ammonia in the Form of Glutamine
54. 59
1.Glutamate –
formed from cellular transamination.
major role in inter-organ transport of NH3.
Conc. of glutamate in blood is 10 times high than other AAs.
Glutamate from blood reaches Liver.
Here ,GDH liberates NH3 from Glutamate .
2. Glutamine from Brain .
Brain is very sensitive to NH3, so, it has a special mechanism for immediate detoxification.
Glutamate – charged
- cannot pass .
Glutamine – neutral, non-toxic
- can pass through cell membrane.
55. 60
• Even a marginal elevation in the blood ammonia conc. is harmful to the
brain.
• when ammonia accumulates in the body, results in…
• slurring of speech,
• Blurring of the vision, Tremors.
• May lead to coma and finally,
• Death, if not corrected.
• Elevation in blood NH3 level may be genetic or acquired.
• Impairment in urea synthesis leads to hyperammonemia.
• And cause hepatic coma and mental retardation.
• The acquired hyperammonemia may be due to hepatitis, alcoholism
etc.
56. 61
Functions of Ammonia
• Ammonia is essential for the synthesis of non-essential amino acids,
purines, pyrimidines, amino sugars & aspargine.
• Ammonium ions are very important to maintain acid-base balance
of the body.
The proteins on degradation (proteolysis)
release individual amino acids. Amino acids are
not just the structural components of proteins.
In contrast to carbohydrates and fat whose major function is to provide energy, the primary role of
amino acids is to serve as building blocks of synthesis of tissue protein and other nitrogen
containing compounds