This document provides information about protein metabolism. It discusses that proteins undergo constant breakdown and resynthesis through protein turnover. The amino acids released are utilized for synthesis of new proteins, nucleic acids, and other biomolecules. Excess amino acids are converted to urea which is excreted in urine. The urea cycle occurring in the liver is the major route for ammonia detoxification. Deficiencies of urea cycle enzymes can cause hyperammonemia.
Metabolism of amino acids (general metabolism)Ashok Katta
Metabolism of amino acids (general metabolism).
Part - I of amino acid metabolism.
This presentation covers Transamination, deamination, formation and Transport of Ammoniaand etc.
Proteins are the macromolecules responsible for the biological processes in the cell. They consist at their most basic level of a chain of amino acids, determined by the sequence of nucleotides in a gene. Depending on the amino acid sequence (different amino acids have different biochemical properties) and interactions with their environment, proteins fold into a three-dimensional structure, which allows them to interact with other proteins and molecules and perform their function
Metabolism of amino acids (general metabolism)Ashok Katta
Metabolism of amino acids (general metabolism).
Part - I of amino acid metabolism.
This presentation covers Transamination, deamination, formation and Transport of Ammoniaand etc.
Proteins are the macromolecules responsible for the biological processes in the cell. They consist at their most basic level of a chain of amino acids, determined by the sequence of nucleotides in a gene. Depending on the amino acid sequence (different amino acids have different biochemical properties) and interactions with their environment, proteins fold into a three-dimensional structure, which allows them to interact with other proteins and molecules and perform their function
In this slide deck I show on the basis of meta-analyses how red and processed meat is linked to diabetes, strokes, heart disease and cancer whereas dairy is not.
Nutritional assessment in chronic liver diseaseShaimaa Elkholy
Protein Energy Malnutrition (PEM) is highly prevalent among patients with chronic liver disease. One of the problems is how to assess these patients nutritionally. yet no standard golden rule for their nutritional assessment.
This presentation has detailed information on glycolysis. each step is explained in detail. there are certain videos which i have taken from youtube. if these videos are not viewable u can refer to shomus biology glycolysis videos. u will get a detailed info there.
lehninger 3rd edition is also very good for the structures
Happy studying :)
structure of proteins
definition of Digestion
sources of Proteins --> EXOGENEOUS SOURCES 50-100g/day and ENDOGENEOUS SOURCES 30-100g/day
Proteins DEGRADED BY --> HYDROLASES specifically PEPTIDASES(ENDOPEPTIDASES & EXOPEPTIDASES)
1. Gastric Digestion of Proteins
2. Pancreatic Digestion of Proteins
3. Digestion of Proteins by Small Intestine Enzymes
Absorption of Amino ACids by Na+Dependent, Na+ Independent, Meister Cycle or gama-glutamyl cycle
designed for undergraduate level teaching of nitrogen metabolism in biochemistry. this is first in the series of three lectures. ideal for MBBS level teaching
Hormonal regulation of proteins metabolismOMEED AKBAR
During digestion, proteins are hydrolyzed into amino acids. Amino acids are absorbed by the capillaries of villi and enter the liver via the hepatic portal vein.
Essential Textbook of Biochemistry For Nursing (B.Sc.Nursing & PBN)Tapeshwar Yadav
I have relished teaching Biochemistry during my more than Ten years teaching experience in a medical, dental, nursing and health science colleges. It was because of constant inspiration from my students that I could come up with Essentials Textbook of Biochemistry for Nursing book, which hopefully would meet the inadequacies the students face in other books. In this age when the concepts in this subject are constantly changing, this book attempts to summarise the fundamentals and current state of knowledge in Biochemistry.
Biochemistry has been primarily written for the students of B.Sc. Nursing & Post Basic of Nursing (PBN) in such a way that it will also be suitable for General Medicine, Radiography, Physiotherapy, Ayurveda, Optometry, Dental and Nursing. This book can also be used as Reference for B.Sc. MLT, Bachelor of Pharmacy (B. PHARMA), Bachelor of Public Health (BPH), Bachelor of Physiotherapy (BPT), B. Ophthalmology, Bachelor of Radiography (BRT) and Biomedical Engineering students of Tribhuvan University, Purbanchal University, Kathmandu University and Pokhara University. Similarly, it will be equally useful for all the teachers, academic writers and those who are involved directly or indirectly in teaching and practising Health Sciences.
This is a basic book on Essential Textbook of Biochemistry for Nursing. The book thoroughly discusses some of the major concepts of Biochemistry and provides adequate information to help the students understand its implications in various areas of the subject. Furthermore, the book aims at equipping the students with practical cum theoretical skills. The book covers almost all the topics which have been prescribed in the Syllabus.
This is an introductory course to Biochemistry and is about medical biochemistry including the biochemical processes of - digestion & absorption of foods, metabolism of different kinds of foods & their disturbance effects in our body together with the physiological roles of different kinds of vitamins & enzymes.
The book consists of Theory as well as Practical portion. The author has tried his best to make all the concepts of each unit as lucid and simple as required for the students with supportive examples, samples, diagrams, clinical disorders and practical works. The ultimate purpose of this book is to equip the reader with comprehensive knowledge in Biochemistry with reference to basic as well as clinical aspects.
At last, I have made every effort to make the book error free, I am under no illusion. I expect constructive comments and suggestions from learners and teachers who use this book which will obviously help me in improving the future edition of the book.
Amino acids are a group of organic compounds containing two functional groups amino and carboxyl. The amino group (-NH2) is basic while the carboxyl group (-COOH) is acidic in nature.
The plasma in the liquid medium of blood (55%) in which the cell components namely Erythrocytes, Leucocytes and Platelets are suspended.
If anticoagulated blood is centrifuged, the plasma separates out as a supernatant while the cells remain at the bottom.
Plasma consists of water electrolytes metabolites nutrients proteins and hormones.
Most of the plasma proteins are synthesized in the liver.
Plasma proteins are separated by electrophoresis.
The word protein is derived from the Greek word ‘Proteios’ which means holding the first place. Berzelius (Swedish chemist) suggested the name proteins to the group of organic compounds that are important to life.
Proteins are the most abundant organic molecules of the living system.
They occur in every part of the cell and constitute about 50% of the cellular dry weight.
Proteins form the fundamental basis of structure and function of life.
Out of the total dry body weight, 3/4th are made up of proteins.
Proteins are used for body building; all the major structural and functional aspects of the body are carried out by protein molecules.
Proteins are high molecular weight polypeptides containing α-amino acids joined together by peptide linkage (-CO-NH).
The endocrine system consists of a network of ductless glands that secrete chemicals (called hormones) that affect the function of specific organs within the body, thus regulating many of the intricate functions of the body itself.
These ductless glands secrete their hormones directly into the bloodstream, as opposed to releasing them externally through ducts (as do the sweat glands and the oil glands).
The field of medicine that deals with the study of the endocrine system and the treatment of the diseases and disorders of the endocrine system is known as endocrinology.
The physician who specializes in the medical practice of endocrinology
is known as an endocrinologist.
Carbohydrates are the most abundant organic molecules in nature.
They are commonly known as saccharides or sugars.
They are primarily composed of the elements carbon, hydrogen and oxygen.
The name carbohydrate literally means “hydrates of carbon”.
Carbohydrates are widely distributed in nature in plants and animals.
The most important carbohydrate found in plants is starch.
It occurs abundantly in roots, tubers, vegetables and grains. The carbohydrate found in animals is glycogen.
It is a storage form of carbohydrate present in liver and muscles, which serves as important sources of energy for vital activities.
This field combines biology as well as chemistry to study the chemical structure of a living organism
Biochemistry is a basic science which deals with chemical nature and chemical behaviour of living matter and with the reactions and processes they undergo.
“The branch of science dealing with the study of all the life processes such as control and coordination within a living organism is called Biochemistry”
Medical parasitology : study of parasites that infect human, diseases caused by them, clinical picture, their diagnosis, treatment and prevention as well as controls.
It involves drug development, epidemiological studies and study of zoonoses.
To know various terms related to parasitology.
To know about general parasites and parasitic infections.
To get knowledge about laboratory diagnosis and its importance.
To gain idea about general epidemiological aspects of parasites that affect human.
Apply basic methods of specimen collection , preservation and processing in lab.
To prevent ourselves from these infections and apply control measures.
Microbiology is the study of
living organisms of microscopic
size which includes bacteria ,
Fungi , Algae , Protozoa and Viruses. It is concerned with the forms, structure , reproduction , physiology , metabolism and classification.
Principle Of Microbiology
Medical microbiology deals with the causative agent of the infectious disease of the human , the ways in which they produce disease in the body and essential information for diagnosis and treatment.
Hematology is the branch of medicine, that is concerned with the study of blood, blood forming organs and blood diseases. It includes study of etiology, diagnosis, treatment, prognosis and prevention of blood diseases .
After the completion of this presentation we will know about:
What is hematology and its purpose.
hematology laboratory.
Blood and its compositions and collections
Hematology lab equipment's
Some hematological tests , disease and hazards too.
Biochemistry is the study of the structure and function of biological molecules such as proteins, nucleic acids, carbohydrates and lipids.
Biochemistry is the study of the chemistry of living things. This includes organic molecules and their chemical reactions.
Biochemistry deals with body substance like enzymes, carbohydrates, amino acids, fats, proteins, hormones, DNA, RNA, pigments etc.
The major objective of biochemistry is the complete understanding of all chemical processes associated with living cells at the molecular level. Some of the objectives can be listed as follows:
1. Isolation, structural elucidation and the determination of mode of action of biomolecules.
2. Identification of disease mechanisms.
3. Study of in born errors of metabolism.
4. Study of oncogenes in cancer cells.
5. The relationship of biochemistry with the genetics, physiology, immunology, pharmacology, toxicology etc.
Biochemistry is related to almost all the life sciences and without biochemistry background and knowledge, a through understanding of health and well-being is not possible.
It is a well known fact that metal ions have a profound effect on cellular processes
The importance or the role that ions play in cellular activity can be gauged by the fact that most cells maintain a very critical Na+ & k+ balance between the extracellular and the intracellular spaces.
Any distribution in this critical balance is to the cellular metabolism through a drastic change in the osmotic pressure resulting in cellular swelling.
An ISE operates an exactly the same principles as a PH electrode
In fact, a PH electrode is a type of ion selective electrode sensitive to hydrogen ion.
Just like a PH electrode, the electrode body contains a reference solution and an metal reference electrode
Safety cabinets are intended to protect a laboratory worker from aerosols and airborne particles.
They will not protect the person from spillages and the consequences of mishandling and poor technique.
Aerosol particles of less than 5 µm in diameter and small droplets of 5–100 µm in diameter are not visible to the naked eye.
The laboratory worker is generally not aware that such particles are being generated and may be inhaled or may cross contaminate work surface materials.
BSCs, when properly used, have been shown to be highly effective in reducing laboratory-acquired infections and cross-contaminations of cultures due to aerosol exposures. BSCs also protect the environment.
Most BSCs use high efficiency particulate air (HEPA) filters in the exhaust and supply systems.
The exception is a Class I BSC, which does not have HEPA filtered supply air.
The application of knowledge, techniques and equipment to prevent a personal laboratory and environmental exposure to potentially infectious agents or biohazard is known as biosafety.
Biosafety defines the containment conditions under which infectious agents can be safely manipulated.
The objective of containment is to confine biohazard and to reduce the potential exposure of the laboratory worker, persons outside of the laboratory, and the environment to potentially infectious agents.
A pipette (also called a point or a pipettor) is a laboratory instrument used to transfer a measured volume of liquid.
Pipettes are commonly used in chemistry and molecular biology research as well as clinical biochemistry tests.
Pipettes come in several designs for various purposes with different levels of accuracy and precision, from single piece flexible plastic transfer pipettes to more complex adjustable or electronic pipettes.
A pipette works by creating a vacuum above the liquid-holding chamber and selectively releasing this vacuum to draw and dispense liquid.
Safe Use and Storage of Chemicals and ReagentsTapeshwar Yadav
Even in the smallest laboratory, dangerous chemicals are used directly or incorporated into stains and reagents.
Hence the correct handling and storage of hazardous chemicals is essential to prevent injury and damage.
In addition to this, to reduce accidents caused by chemicals, labeling is very important.
Laboratory Hazards, Accidents and Safety RulesTapeshwar Yadav
Injury, damage and loss by fire can be minimized when laboratory staff:
Understand how fires are caused and spread;
Reduce the risk of fire by following fire safety regulations at all times;
Know what to do if there is a fire in their laboratory;
Know how to use fire fighting equipment;
Know how to apply emergency First Aid, for burns.
Revised Curriculum of Certificate in Medical Laboratory Technology(CMLT) by C...Tapeshwar Yadav
This curriculum of 3 years Certificate in Medical Laboratory Technology is designed to produce middle level skilled laboratory personnel equipped with knowledge, skills and attitudes of medical laboratory with a view to provide diagnostic, curative, preventive and promotive laboratory services to the community. Such technicians collect specimens, process, and perform tests to analyze body fluids, tissue, and other substances. The graduates perform lab procedures and maintain instruments. The graduates are expected to perform tests that help other healthcare professionals such as physicians to detect, diagnose, and treat diseases.
The program extends over three academic years. The first year course focuses on basic science and foundational subjects, the second year course focuses on theory and practical parts of basic medical laboratory subjects. Simultaneously the third year is given to the application of learned skills and knowledge in the comprehensive practical settings, in hospitals and medical laboratory. The graduates will have career opportunities in hospitals, diagnostic laboratories, clinics, industry and physicians' offices, research centers, blood bank, crime investigating laboratories etc. It is based on the code of conduct of Nepal Health professional Council.
Clinical Pathology is the application of laboratory techniques to find out the cause of disease. Clinical pathology laboratory involves all aspect of the medicine ranging from the field of biochemistry, microbiology, Parasitology, haematology, immunology and cytogenetics etc. Clinical pathology laboratory perform qualitative and quantitative analysis of body fluid such as urine, blood, CSF, sputum, other body fluid such as synovial fluid, peritoneal fluid, pericardial fluid and plural fluid. These determinations are useful in diagnosing various clinical conditions such as diabetes mellitus, jaundice, gout, hyperlipidemia, pancreatitis, rickets, etc. The clinical pathological tests are very useful in determining the severity of diseases of many organs such as liver, stomach, heart, kidneys, brain as well as the endocrine disorders and related status of acid-base balance of the body. The clinical pathology tests, in relation to the various clinical conditions can be applicable for:-
1) Reveal the causes of the diseases
2) Screen easy diagnosis
3) Suggest effective treatment
4) Assist in monitoring progress of a pathological condition and
5) Help in assessing response to therapy
Modern medicine says that: Your practice of medicine will be as good as your understanding of pathology.
Tom Selleck Health: A Comprehensive Look at the Iconic Actor’s Wellness Journeygreendigital
Tom Selleck, an enduring figure in Hollywood. has captivated audiences for decades with his rugged charm, iconic moustache. and memorable roles in television and film. From his breakout role as Thomas Magnum in Magnum P.I. to his current portrayal of Frank Reagan in Blue Bloods. Selleck's career has spanned over 50 years. But beyond his professional achievements. fans have often been curious about Tom Selleck Health. especially as he has aged in the public eye.
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Introduction
Many have been interested in Tom Selleck health. not only because of his enduring presence on screen but also because of the challenges. and lifestyle choices he has faced and made over the years. This article delves into the various aspects of Tom Selleck health. exploring his fitness regimen, diet, mental health. and the challenges he has encountered as he ages. We'll look at how he maintains his well-being. the health issues he has faced, and his approach to ageing .
Early Life and Career
Childhood and Athletic Beginnings
Tom Selleck was born on January 29, 1945, in Detroit, Michigan, and grew up in Sherman Oaks, California. From an early age, he was involved in sports, particularly basketball. which played a significant role in his physical development. His athletic pursuits continued into college. where he attended the University of Southern California (USC) on a basketball scholarship. This early involvement in sports laid a strong foundation for his physical health and disciplined lifestyle.
Transition to Acting
Selleck's transition from an athlete to an actor came with its physical demands. His first significant role in "Magnum P.I." required him to perform various stunts and maintain a fit appearance. This role, which he played from 1980 to 1988. necessitated a rigorous fitness routine to meet the show's demands. setting the stage for his long-term commitment to health and wellness.
Fitness Regimen
Workout Routine
Tom Selleck health and fitness regimen has evolved. adapting to his changing roles and age. During his "Magnum, P.I." days. Selleck's workouts were intense and focused on building and maintaining muscle mass. His routine included weightlifting, cardiovascular exercises. and specific training for the stunts he performed on the show.
Selleck adjusted his fitness routine as he aged to suit his body's needs. Today, his workouts focus on maintaining flexibility, strength, and cardiovascular health. He incorporates low-impact exercises such as swimming, walking, and light weightlifting. This balanced approach helps him stay fit without putting undue strain on his joints and muscles.
Importance of Flexibility and Mobility
In recent years, Selleck has emphasized the importance of flexibility and mobility in his fitness regimen. Understanding the natural decline in muscle mass and joint flexibility with age. he includes stretching and yoga in his routine. These practices help prevent injuries, improve posture, and maintain mobilit
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Pulmonary Thromboembolism - etilogy, types, medical- Surgical and nursing man...VarunMahajani
Disruption of blood supply to lung alveoli due to blockage of one or more pulmonary blood vessels is called as Pulmonary thromboembolism. In this presentation we will discuss its causes, types and its management in depth.
Recomendações da OMS sobre cuidados maternos e neonatais para uma experiência pós-natal positiva.
Em consonância com os ODS – Objetivos do Desenvolvimento Sustentável e a Estratégia Global para a Saúde das Mulheres, Crianças e Adolescentes, e aplicando uma abordagem baseada nos direitos humanos, os esforços de cuidados pós-natais devem expandir-se para além da cobertura e da simples sobrevivência, de modo a incluir cuidados de qualidade.
Estas diretrizes visam melhorar a qualidade dos cuidados pós-natais essenciais e de rotina prestados às mulheres e aos recém-nascidos, com o objetivo final de melhorar a saúde e o bem-estar materno e neonatal.
Uma “experiência pós-natal positiva” é um resultado importante para todas as mulheres que dão à luz e para os seus recém-nascidos, estabelecendo as bases para a melhoria da saúde e do bem-estar a curto e longo prazo. Uma experiência pós-natal positiva é definida como aquela em que as mulheres, pessoas que gestam, os recém-nascidos, os casais, os pais, os cuidadores e as famílias recebem informação consistente, garantia e apoio de profissionais de saúde motivados; e onde um sistema de saúde flexível e com recursos reconheça as necessidades das mulheres e dos bebês e respeite o seu contexto cultural.
Estas diretrizes consolidadas apresentam algumas recomendações novas e já bem fundamentadas sobre cuidados pós-natais de rotina para mulheres e neonatos que recebem cuidados no pós-parto em unidades de saúde ou na comunidade, independentemente dos recursos disponíveis.
É fornecido um conjunto abrangente de recomendações para cuidados durante o período puerperal, com ênfase nos cuidados essenciais que todas as mulheres e recém-nascidos devem receber, e com a devida atenção à qualidade dos cuidados; isto é, a entrega e a experiência do cuidado recebido. Estas diretrizes atualizam e ampliam as recomendações da OMS de 2014 sobre cuidados pós-natais da mãe e do recém-nascido e complementam as atuais diretrizes da OMS sobre a gestão de complicações pós-natais.
O estabelecimento da amamentação e o manejo das principais intercorrências é contemplada.
Recomendamos muito.
Vamos discutir essas recomendações no nosso curso de pós-graduação em Aleitamento no Instituto Ciclos.
Esta publicação só está disponível em inglês até o momento.
Prof. Marcus Renato de Carvalho
www.agostodourado.com
NVBDCP.pptx Nation vector borne disease control programSapna Thakur
NVBDCP was launched in 2003-2004 . Vector-Borne Disease: Disease that results from an infection transmitted to humans and other animals by blood-feeding arthropods, such as mosquitoes, ticks, and fleas. Examples of vector-borne diseases include Dengue fever, West Nile Virus, Lyme disease, and malaria.
Ozempic: Preoperative Management of Patients on GLP-1 Receptor Agonists Saeid Safari
Preoperative Management of Patients on GLP-1 Receptor Agonists like Ozempic and Semiglutide
ASA GUIDELINE
NYSORA Guideline
2 Case Reports of Gastric Ultrasound
These simplified slides by Dr. Sidra Arshad present an overview of the non-respiratory functions of the respiratory tract.
Learning objectives:
1. Enlist the non-respiratory functions of the respiratory tract
2. Briefly explain how these functions are carried out
3. Discuss the significance of dead space
4. Differentiate between minute ventilation and alveolar ventilation
5. Describe the cough and sneeze reflexes
Study Resources:
1. Chapter 39, Guyton and Hall Textbook of Medical Physiology, 14th edition
2. Chapter 34, Ganong’s Review of Medical Physiology, 26th edition
3. Chapter 17, Human Physiology by Lauralee Sherwood, 9th edition
4. Non-respiratory functions of the lungs https://academic.oup.com/bjaed/article/13/3/98/278874
The prostate is an exocrine gland of the male mammalian reproductive system
It is a walnut-sized gland that forms part of the male reproductive system and is located in front of the rectum and just below the urinary bladder
Function is to store and secrete a clear, slightly alkaline fluid that constitutes 10-30% of the volume of the seminal fluid that along with the spermatozoa, constitutes semen
A healthy human prostate measures (4cm-vertical, by 3cm-horizontal, 2cm ant-post ).
It surrounds the urethra just below the urinary bladder. It has anterior, median, posterior and two lateral lobes
It’s work is regulated by androgens which are responsible for male sex characteristics
Generalised disease of the prostate due to hormonal derangement which leads to non malignant enlargement of the gland (increase in the number of epithelial cells and stromal tissue)to cause compression of the urethra leading to symptoms (LUTS
Lung Cancer: Artificial Intelligence, Synergetics, Complex System Analysis, S...Oleg Kshivets
RESULTS: Overall life span (LS) was 2252.1±1742.5 days and cumulative 5-year survival (5YS) reached 73.2%, 10 years – 64.8%, 20 years – 42.5%. 513 LCP lived more than 5 years (LS=3124.6±1525.6 days), 148 LCP – more than 10 years (LS=5054.4±1504.1 days).199 LCP died because of LC (LS=562.7±374.5 days). 5YS of LCP after bi/lobectomies was significantly superior in comparison with LCP after pneumonectomies (78.1% vs.63.7%, P=0.00001 by log-rank test). AT significantly improved 5YS (66.3% vs. 34.8%) (P=0.00000 by log-rank test) only for LCP with N1-2. Cox modeling displayed that 5YS of LCP significantly depended on: phase transition (PT) early-invasive LC in terms of synergetics, PT N0—N12, cell ratio factors (ratio between cancer cells- CC and blood cells subpopulations), G1-3, histology, glucose, AT, blood cell circuit, prothrombin index, heparin tolerance, recalcification time (P=0.000-0.038). Neural networks, genetic algorithm selection and bootstrap simulation revealed relationships between 5YS and PT early-invasive LC (rank=1), PT N0—N12 (rank=2), thrombocytes/CC (3), erythrocytes/CC (4), eosinophils/CC (5), healthy cells/CC (6), lymphocytes/CC (7), segmented neutrophils/CC (8), stick neutrophils/CC (9), monocytes/CC (10); leucocytes/CC (11). Correct prediction of 5YS was 100% by neural networks computing (area under ROC curve=1.0; error=0.0).
CONCLUSIONS: 5YS of LCP after radical procedures significantly depended on: 1) PT early-invasive cancer; 2) PT N0--N12; 3) cell ratio factors; 4) blood cell circuit; 5) biochemical factors; 6) hemostasis system; 7) AT; 8) LC characteristics; 9) LC cell dynamics; 10) surgery type: lobectomy/pneumonectomy; 11) anthropometric data. Optimal diagnosis and treatment strategies for LC are: 1) screening and early detection of LC; 2) availability of experienced thoracic surgeons because of complexity of radical procedures; 3) aggressive en block surgery and adequate lymph node dissection for completeness; 4) precise prediction; 5) adjuvant chemoimmunoradiotherapy for LCP with unfavorable prognosis.
Prix Galien International 2024 Forum ProgramLevi Shapiro
June 20, 2024, Prix Galien International and Jerusalem Ethics Forum in ROME. Detailed agenda including panels:
- ADVANCES IN CARDIOLOGY: A NEW PARADIGM IS COMING
- WOMEN’S HEALTH: FERTILITY PRESERVATION
- WHAT’S NEW IN THE TREATMENT OF INFECTIOUS,
ONCOLOGICAL AND INFLAMMATORY SKIN DISEASES?
- ARTIFICIAL INTELLIGENCE AND ETHICS
- GENE THERAPY
- BEYOND BORDERS: GLOBAL INITIATIVES FOR DEMOCRATIZING LIFE SCIENCE TECHNOLOGIES AND PROMOTING ACCESS TO HEALTHCARE
- ETHICAL CHALLENGES IN LIFE SCIENCES
- Prix Galien International Awards Ceremony
3. Introduction
Are most abundantly distributed organic compounds.
70 kg man= protein weight constitute 12 kg
Skeleton and connective tissue contains half
Body protein and other half is intracellular.
4. Protein Turnover:
The total amount of protein in the body remains
constant (i.e Rate of protein synthesis is constnt)
Is equal to protein degradation.
This process is called as protein turnover.
300 to 400 Gm/day.
5. Rate turn over
Half-lives Proteins
Hours/Days Digestiive Enzymes &
Plasma proteins.
Months/years Collagen.(Structural Proteins)
Proteins rich in Proline, Glutamate, Serine and
threonine are rapidly degraded and short half-lives
6. AminoAcid pool
Amino acids released by dietary & tissue Protein
Mix with free amino acids of body = Constitutes=100
gm.
Glutamate,Glutamine - 50 %
Essential amino acids - 10%.
Remaining Non-Essential Amino Acids.
7. Proteins rich in Proline,Glutamate,Serine and
Threonine are rapidly degraded and have short half-
lives.
AMINO ACID POOL: -Amino acids released by
1.Dietary and 2.Tissue protein.
8. There is no storage form of amino acids like
Glycogen and Triglycerides.
Excess intake of proteins(Amino acids) are
metabolised then oxidised to provide energy or
converted to glucose or fat.
Amino groups lost as Urea→Excreted.
9. Body Dietary Synthesis
Protein Protein Non-Essential AA
Body Protein Porphyrins
Purines & Pyrimidins
Creatine
Neurotransmitters
UREA
Glucose CO₂ Fattyacids
Glycogen Ketone Bodies
Steroids
AMINO ACID POOL
100 G
10. Digestion of Dietary Proteins
Proteins are too large to be absorbed by
intestine and must be hydrolysed to yield
amino acids,which can beabsorbed.
Proteolytic enzymes responsible for degrading
proteins are produced by 3 different organs:
Stomach,Pancreas and Small intestine.
11.
12.
13. PROTEIN DIGESTION:- Dietary protein-50-100
gm/day.
30-100gm/Day- Endogenous protein from digestive
enzymes.
Dietary proteins are denatured on cooking.
14. Proteins are degraded by hydrolases which
cleave peptide bonds known as peptidases.
Exopeptidases and endopeptidases.
EXOPEPTIDASES ENDO PEPTIDASES
1.Carboxy peptidases. Aspartate Proteinase
2.Amino peptidases. Eg:- Trypsin
3.Tripeptidyl peptidases. Serine Proteinases
4.Dipeptidyl peptidases. Eg:-Trypsin
5.Dipeptidase Cysteine Proteinases
Eg:-Papain
Metallo Proteinases
Eg:-Metal-ion
15. DIGESTION @ Stomach
HCl , pH=2, Due to HCl, secreted by parietal cells.
Denature Protein
Destroys microorganisms.
Pepsin = Pepsinogen HCl Pepsin.
16. ABSORPTION :- Occurs in small intestine of infants
immediately after birth. This process is known as
PINOCYTOSIS.
Direct transfer is useful for taking up of maternal
γ Globulins.
17. ADULTS:-Direct transfer of intact protein (or) poly
peptide in body elicits Antibody formation(Food
allergy).
NONTROPICAL SPURE:-Tripeptde digestionof wheat
Glutemate stimulates antibody production.
CELIAC DISEASE in children same phenomena has
been obsreved.
18. Abnormality of protein digestion:-HARTNUP’S
disease is due inability of intestinal absorption of
neutral amino acids.
PROTEIN TURNOVER:-Continuous degradation and
resynthesis occurs in all cellular proteins.
Adults degrade 1-2% of their body protein
daily(muscle protein).
75-80% are utilized for protein synthesis.
20-25% for urea.
19.
20. Proteins are degraded at varying rates.
1.High mean rates of protein degradation occurs in
uterine tissue during pregnacy.
2.During strvation ,skeletal muscle protein
degradation is ↑.
21.
22.
23. TRANSMINATION is defined as a process in
which amino group is transferred from an Aminoacid
to Ketoacid to form an corresponding Aminoacid that
itself is forming Ketoacid with out liberation of
ammonia.
The enzymes catalyzing the reaction as a group are
known as AMINO TRANSFERASES.
All amino Transferases require coenzyme –
Pyridoxal phosphate-B6.
25. All amino acids ,except LYSINE,THREONINE
participate in Transmination.
The reaction is Cytoplasmic and takes place in
liver.
Transminases are induced by Glucocorticoids
which promotes Gluconeogenesis.
26. Clinical significance:- ↑Levels in plasma indicates
damage to cells rich in these enzymes.
SGPT↑ :- Toxic Hepatisis.
Viral Hepatisis
Cirrhosis.
SGOT:- Myocardial infarction,Pulmonary disorders.
Function:- Transmination is useful synthesis of
Non- Essential Aminoacids.
Major oxidative deamination is catalysed.
Glutamate DeHydrogenase (GDH)
It is a mitochondrial enzyme in Liver.
30. All amino acids, amino groups are funnelled into
glutamate.
High Protein Diet = High Ammonia formation.
When energy levels are low amino acid degradation
by GDH is High,Provides α-KG for TCA cycle.
Allosteric regulators=
ATP,GTP=Inhibitors
ADP,GDP=Activators
31. NON-OXIDATIVE DEAMINATION
1.Enzymes that acts as dehydratases forms
corresponding keto acid and ammonia.
Threonine Deaminase Keto Butyrate +NH₃
2.Transulfuration:
Cysteine Pyruvate+NH₃ + H₂S
3.Histidine undergoes non-oxidative deamination
by histidase.
4.Glutamine Glutaminase* Glutamate + NH₃
Aspargine Asparginase* Aspartate + NH₃.
These enzymes have been utilized as Anti-Tumor
Agents.
*Acts as anti tumor agents
32. Metabolism of Ammmonia:-
SOURCES OF AMMONIA :-
Amino acids Synthesises Protein,
Protein degraded to Amino acids.
From Liver : a) Transamination
b) Oxidative deamination
From Kidney : Glutaminase reaction
From Intestine : By Bacterial action
From Diet : Amines
From Catabolism : Purines (Adenine)
Pyrimidines (Cytosine)
From Non–oxidative: Deamination : Aminoacids
33. UTILIZATION OF AMMONIA:-
Glutamate+ Ammonia Glutamine.
Glutamine synthetase- Liver, Brain and Kidney.
Brain :- Major mechanism for removal of
Ammonia is Glutamate formation.
αKG+NH3+NADPH+H⁺ Glutamate+NADP⁺
Glutamate may be considered as a major transport
form of NH₃ from tissue to liver.Concentration of
Glutamate in blood is 10 times more than other
amino acids.
34. 3 Important disposal route of ammonia is formation
of Urea.
End product Amino Nitrogen
In mammals is Urea - Ureotelic.
Fishes is Ammonia - Ammonotelic.
Birds & Reptiles is Uric acid - Uricotelic
35. TRANSPORT OF AMMONIA:-
Ammonia is constantly produed in tissues.
Plasma ammonia - 10-20 μg /dl.
Elevated levels cause symptoms of ammonia
intoxication.
SYMPTOMS:-Tremor, Slurring of speech, Blurring of
vision→Coma and death.
37. HEREDITARY HYPER AMMONEMIA:-
All inherited deficiencies of UREA CYCLE ENZYMES
result in HYPER AMMONEMIA.
Prevalence is 1 in 30000 individuals.
38.
39. UREA CYCLE
1.Enzymes of Urea cycle
2.Regulation of Urea cycle
3.Energetics of Urea cycle
4.Clinical significance of blood Urea
5.Disorders of Urea cycle
40. Urea cycle is also called as Krebs-Henseleit or
Ornithine cycle.
Site: Liver
Urea synthesized in Liver, released into Blood, cleared
by Kidneys.
Urea cycle is devided into Five steps.
41.
42. Two nitrogen atoms of urea are derived from
ammonia and alpha amino group of aspartic
acid.
One mol of urea synthesis requires 4 mol of ATP.
Step 1 in Mitochondria:-
CO₂ + Ammonia + 2 ATP Carbamoyl
Phosphate+2ADP+PPi
Carbamoyl phosphate synthase 1(CPS-1)
It is a Mitochondrial enzyme,
Allosteric activator is N-Acetyl Glutamate.
43. Step 2. Formation of Citrulline
Carbamoyal phosphate +Ornithine
Ornithine Transcarbamoylase
Citrulline + Pi
Ornithine trans carbmoylase is also a Mitochondrial
enzyme
44. This step onwards the reactions occurred in
CYTOPLASM
Step3- Formation of Argininosuccinate.
Citrulline + Aspartate + ATP
Argininosuccinate synthase
Argininosuccinate + AMP + PPi
46. 2.Regulation of Urea Cycle :
a) Carbamoyl Phosphate Synthase-1:
Allosteric activator –N-acetylglutamate.
More glutamate, more N-acetylglutamate,
more CPS-1 activity, leads more Urea synthesis.
b) During starvation, Urea cycle enzyme activities are
increased to meet the demands of increased rate of
protein catabolism.
47. 3 Energetics of Urea cycle:
ATP UTILIZED
Carbamoyal phosphate synthase -2ATP
Argininosuccinate synthase -2ATP
ATP GENERATED
Fumarate- Malate- MDH +3ATP
Net Energy expenditure -1ATP
48. Clinical significance of Blood Urea:
Normal Blood Urea = 15 - 40 mgs %
Normal Urine Urea = 15 - 30 grams/day
Increased Blood Urea levels:
Prerenal causes
a) High protein diet
b) Increased protein catabolism –starvation
c) Gastro-intestinal haemorrhage.
49. Renal causes
a) Chronic renal failure
B) Acute glomerulonephritis
c) Nephroscelerosis.
Postrenal causes
a) Renal stone
B) Prostate enlargement
c) Malignant stricture
50. Decreased Blood Urea Levels:
a) Low protein diet.
b) Liver diseases
c) Water retention.
51. DISORDERS OF UREA CYCLE:
a) Hyperammonemia - Type I
-Enzyme Defeciency - CPS 1
-Symptoms - Increased blood ammonia (↑ NH₄)
Mental retardation
Autosomal recessive .
52. HYPER AMMONEMIA Type II
Enzyme deficiency –Ornithine transcarbamoylase
↑ NH₃ in Blood; Glutamine ↑ in C.S.F, urine, blood.
X-Linked inheritance.
Orotic aciduria, Mental retardation.