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1
VACCINES
2
•Introduction
•Types
Whole-Organism Vaccines
Purified Macromolecules as Vaccines
Recombinant vaccine
DNA vaccine
Multivalent Subunit Vaccines
•Mechanism
•Effectiveness
•Manufacturing Strategies
•Risk associated with vaccines
•Recent research
•Conclusion
•Reference
Conte
nts
•British physician Edward Jenner, who in 1796 used the cowpox virus (Latin variola
vaccinia) to confer protection against smallpox.
•In 1885 the French microbiologist Louis Pasteur and Emile Roux developed the
first vaccine against rabies.
Introdu
ction
A vaccine is a biological preparation that improves immunity to a particular disease. It
contains certain agents that not only resembles a disease-causing microorganism but it also
stimulates body’s immune sustem recognize the foreign agents.
History:
Definition:
(Ref: www.wikipedia.org, www.britannica.com, www.pathmicro.med.sc.edu)
3
4
Vaccines are dead or inactivated organisms or purified products derived from
them. There are several types of vaccines in use. They are:
Typ
es
• Whole-Organism Vaccines
Killed
Attenuated
• Purified Macromolecules as Vaccines
Toxoids
Capsular polysaccharides
Recombinant microbial antigens/Surface antigens
• Recombinant vaccine
• DNA vaccine
• Multivalent Subunit Vaccines (Ref: Kuby, book for Immunology)
5
Many of the common vaccines currently in use consist of inactivated (killed) or live but
attenuated (avirulent) bacterial cells or viral particles.
Whole-Organism
Vaccines
 Killed/Inactivated.
 Attenuated.
Killed/ Inactivated: Some vaccines contain killed, but previously virulent, micro-
organisms that have been destroyed with chemicals, heat, radioactivity or antibiotics.
Attenuated: Some vaccines contain live, attenuated microorganisms. Many of these are
live viruses that have been cultivated under conditions that disable their virulent
properties, or which use closely related but less dangerous organisms to produce a broad
immune response.
(Ref: Kuby, book for Immunology)
6
Disease or pathogen Type of vaccine
WHOLE ORGANISMS
Bacterial cells
Anthrax
Cholera
Pertussis*
Plague
Tuberculosis
Typhoid
Inactivated
Inactivated
Inactivated
Inactivated
Live attenuated
Live attenuated
Classification of common
vaccines for humans:
(Ref: Kuby, book for Immunology)
7
Disease or pathogen Type of vaccine
Viral particles
Hepatitis A
Influenza
Measles
Polio (Sabin)
Polio (Salk)
Rabies
Rotavirus
Varicella zoster (chickenpox)
Yellow fever
Inactivated
Inactivated
Live attenuated
Live attenuated
Inactivated
Inactivated
Live attenuated
Live attenuated
Live attenuated
(Ref: Kuby, book for Immunology)
8
• Inactivated exotoxins.
• Capsular polysaccharides.
• Recombinant microbial antigens/Surface antigens.
Purified Macromolecules as Vaccine
(Ref: Kuby, Book for Immunology)
9
Inactivated
exotoxins/Toxoid
• Toxoids are vaccines which consist of exotoxins that have been inactivated,
either by heat or chemicals. These vaccines are intended to build immunity
against the toxins, but not necessarily the bacteria that produce the toxins.
• Some examples are botulinum antitoxin and diphtheria antitoxin.
Fig: Modification of toxin to toxoid
(Ref: Kuby, www2a.cdc.gov)
10
• The virulence of some pathogenic bacteria depends primarily on the anti
phagocytic properties of their hydrophilic polysaccharide capsule.
• Coating of the capsule with antibodies and or complement greatly increases
the ability of macrophages and neutrophils to phagocytose such pathogens.
• The current vaccine for Streptococcus pneumoniae, which causes
pneumococcal pneumonia, consists of 23 antigenically different capsular
polysaccharides.
Capsular polysaccharides
(Ref: Kuby, www2a.cdc.gov)
11
• The gene encoding any immunogenic protein can be cloned and expressed
in bacterial, yeast, or mammalian cells using recombinant DNA technology.
• The first such recombinant antigen vaccine approved for human use is the
hepatitis B vaccine. This vaccine was developed by cloning the gene for the
major surface antigen of hepatitis B virus (HBsAg) and expressing it in yeast
cells.
Recombinant microbial
antigens/Surface antigen
(Ref: Kuby, book for Immunology)
12
(Ref: Kuby, book for Immunology)
Recombinant Vaccines:
Fig: Production of vaccinia vector vaccine.
13
DNA Vaccines:
Fig: Use of DNA vaccines raises both humoral and cellular immunity
14
Multivalent Subunit
Vaccines
Multivalent subunit
vaccines
Solid matrix–
antibody-antigen
(SMAA) complex
Detergent to
protein antigens
(Ref: Kuby, book for Immunology)
15
(Ref: Kuby, book for Immunology)
Solid matrix–antibody-antigen
(SMAA) complex
Fig: Solid matrix Antigen
16
(Ref: Kuby, book for Immunology
Detergent to protein antigens
Fig: b. Detergent extracted membrane antigens or antigenic peptides
c. ISCOM delivery of antigen into cell
17
Mechanism of a vaccine
Fig: Mechanism of vaccine
(Ref: www.pathmicro.med.sc.edu)
18
Vaccines do not guarantee complete protection from a disease.
Adjuvants:
•An adjuvant (Latin, adiuvare: to aid) is a pharmacological or immunological
agent that modifies the effect of other agents, such as a drug or vaccine. They
are often included in vaccines to enhance the recipient's immune response to a
supplied antigen, while keeping the injected foreign material to a minimum.
Effectiv
eness
(Ref: www.wikipedia.org)
19
• The primary risk associated with vaccines, especially vaccines that utilize live
organisms, is that the vaccine itself causes illness.
•Another risk is that the vaccine may behave as a super antigen and over stimulate the
immune system.
•Yet a third risk is that some individuals may have an allergic reaction to the vaccine,
especially vaccines produced in Embryonated chicken eggs and in transgenic plants.
Risks associated
with vaccinesVaccines also have some sort of risks, like:
(Ref: www.wikipedia.org)
20
• Approaches for designing a preventive HIV vaccine.
• Vaccine against Dengue Vaccine.
• NIH Scientists Identify New HIV-Inhibiting Protein.
•NIH Scientists Find Cause of Rare Immune Disease: Genetic Mutation Leads to Cold
Allergy, Immune Deficiency and Autoimmunity.
• NIH Found a Gene That May Play a Role in Type 1 Diabetes.
Recent Research:
(Ref: www.niaid.nih.gov)
21
Vaccines are one of the most effective health interventions
ever developed. Three types of vaccines are currently used in humans: attenuated
(avirulent) microorganisms, inactivated (killed) microorganisms, or purified
macromolecules. Recombinant vector vaccine and Plasmid DNA vaccines are also used.
They induce both humoral and cell-mediated immunity. Some boosters (called adjuvants)
are also used in association with vaccines for increasing the immune response. As the
vaccines have a lot of benefits, they do carry some harmful effects too.
Conclusion
22
Source:
•Janes Kuby, 2007, Vaccines, Immunology, W.H. Freeman and Company,
Newyork, sixth Edition, Pg. 413- 428.
•Satyanarayana U., 2010, Vacines, Biotechnolgy, BOOK’S AND ALLIED (P)
Ltd, Kolkata, sixth edition, Pg. 211-212.
Net Source:
• www.wikipedia.org
• www.britannica.com
• www.pathmicro.med.sc.edu
• www.mpi-magdeburg.mpg.de
• www.sc.edu
• www.niaid.nih.gov
Refere
nce

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Vaccines

  • 2. 2 •Introduction •Types Whole-Organism Vaccines Purified Macromolecules as Vaccines Recombinant vaccine DNA vaccine Multivalent Subunit Vaccines •Mechanism •Effectiveness •Manufacturing Strategies •Risk associated with vaccines •Recent research •Conclusion •Reference Conte nts
  • 3. •British physician Edward Jenner, who in 1796 used the cowpox virus (Latin variola vaccinia) to confer protection against smallpox. •In 1885 the French microbiologist Louis Pasteur and Emile Roux developed the first vaccine against rabies. Introdu ction A vaccine is a biological preparation that improves immunity to a particular disease. It contains certain agents that not only resembles a disease-causing microorganism but it also stimulates body’s immune sustem recognize the foreign agents. History: Definition: (Ref: www.wikipedia.org, www.britannica.com, www.pathmicro.med.sc.edu) 3
  • 4. 4 Vaccines are dead or inactivated organisms or purified products derived from them. There are several types of vaccines in use. They are: Typ es • Whole-Organism Vaccines Killed Attenuated • Purified Macromolecules as Vaccines Toxoids Capsular polysaccharides Recombinant microbial antigens/Surface antigens • Recombinant vaccine • DNA vaccine • Multivalent Subunit Vaccines (Ref: Kuby, book for Immunology)
  • 5. 5 Many of the common vaccines currently in use consist of inactivated (killed) or live but attenuated (avirulent) bacterial cells or viral particles. Whole-Organism Vaccines  Killed/Inactivated.  Attenuated. Killed/ Inactivated: Some vaccines contain killed, but previously virulent, micro- organisms that have been destroyed with chemicals, heat, radioactivity or antibiotics. Attenuated: Some vaccines contain live, attenuated microorganisms. Many of these are live viruses that have been cultivated under conditions that disable their virulent properties, or which use closely related but less dangerous organisms to produce a broad immune response. (Ref: Kuby, book for Immunology)
  • 6. 6 Disease or pathogen Type of vaccine WHOLE ORGANISMS Bacterial cells Anthrax Cholera Pertussis* Plague Tuberculosis Typhoid Inactivated Inactivated Inactivated Inactivated Live attenuated Live attenuated Classification of common vaccines for humans: (Ref: Kuby, book for Immunology)
  • 7. 7 Disease or pathogen Type of vaccine Viral particles Hepatitis A Influenza Measles Polio (Sabin) Polio (Salk) Rabies Rotavirus Varicella zoster (chickenpox) Yellow fever Inactivated Inactivated Live attenuated Live attenuated Inactivated Inactivated Live attenuated Live attenuated Live attenuated (Ref: Kuby, book for Immunology)
  • 8. 8 • Inactivated exotoxins. • Capsular polysaccharides. • Recombinant microbial antigens/Surface antigens. Purified Macromolecules as Vaccine (Ref: Kuby, Book for Immunology)
  • 9. 9 Inactivated exotoxins/Toxoid • Toxoids are vaccines which consist of exotoxins that have been inactivated, either by heat or chemicals. These vaccines are intended to build immunity against the toxins, but not necessarily the bacteria that produce the toxins. • Some examples are botulinum antitoxin and diphtheria antitoxin. Fig: Modification of toxin to toxoid (Ref: Kuby, www2a.cdc.gov)
  • 10. 10 • The virulence of some pathogenic bacteria depends primarily on the anti phagocytic properties of their hydrophilic polysaccharide capsule. • Coating of the capsule with antibodies and or complement greatly increases the ability of macrophages and neutrophils to phagocytose such pathogens. • The current vaccine for Streptococcus pneumoniae, which causes pneumococcal pneumonia, consists of 23 antigenically different capsular polysaccharides. Capsular polysaccharides (Ref: Kuby, www2a.cdc.gov)
  • 11. 11 • The gene encoding any immunogenic protein can be cloned and expressed in bacterial, yeast, or mammalian cells using recombinant DNA technology. • The first such recombinant antigen vaccine approved for human use is the hepatitis B vaccine. This vaccine was developed by cloning the gene for the major surface antigen of hepatitis B virus (HBsAg) and expressing it in yeast cells. Recombinant microbial antigens/Surface antigen (Ref: Kuby, book for Immunology)
  • 12. 12 (Ref: Kuby, book for Immunology) Recombinant Vaccines: Fig: Production of vaccinia vector vaccine.
  • 13. 13 DNA Vaccines: Fig: Use of DNA vaccines raises both humoral and cellular immunity
  • 14. 14 Multivalent Subunit Vaccines Multivalent subunit vaccines Solid matrix– antibody-antigen (SMAA) complex Detergent to protein antigens (Ref: Kuby, book for Immunology)
  • 15. 15 (Ref: Kuby, book for Immunology) Solid matrix–antibody-antigen (SMAA) complex Fig: Solid matrix Antigen
  • 16. 16 (Ref: Kuby, book for Immunology Detergent to protein antigens Fig: b. Detergent extracted membrane antigens or antigenic peptides c. ISCOM delivery of antigen into cell
  • 17. 17 Mechanism of a vaccine Fig: Mechanism of vaccine (Ref: www.pathmicro.med.sc.edu)
  • 18. 18 Vaccines do not guarantee complete protection from a disease. Adjuvants: •An adjuvant (Latin, adiuvare: to aid) is a pharmacological or immunological agent that modifies the effect of other agents, such as a drug or vaccine. They are often included in vaccines to enhance the recipient's immune response to a supplied antigen, while keeping the injected foreign material to a minimum. Effectiv eness (Ref: www.wikipedia.org)
  • 19. 19 • The primary risk associated with vaccines, especially vaccines that utilize live organisms, is that the vaccine itself causes illness. •Another risk is that the vaccine may behave as a super antigen and over stimulate the immune system. •Yet a third risk is that some individuals may have an allergic reaction to the vaccine, especially vaccines produced in Embryonated chicken eggs and in transgenic plants. Risks associated with vaccinesVaccines also have some sort of risks, like: (Ref: www.wikipedia.org)
  • 20. 20 • Approaches for designing a preventive HIV vaccine. • Vaccine against Dengue Vaccine. • NIH Scientists Identify New HIV-Inhibiting Protein. •NIH Scientists Find Cause of Rare Immune Disease: Genetic Mutation Leads to Cold Allergy, Immune Deficiency and Autoimmunity. • NIH Found a Gene That May Play a Role in Type 1 Diabetes. Recent Research: (Ref: www.niaid.nih.gov)
  • 21. 21 Vaccines are one of the most effective health interventions ever developed. Three types of vaccines are currently used in humans: attenuated (avirulent) microorganisms, inactivated (killed) microorganisms, or purified macromolecules. Recombinant vector vaccine and Plasmid DNA vaccines are also used. They induce both humoral and cell-mediated immunity. Some boosters (called adjuvants) are also used in association with vaccines for increasing the immune response. As the vaccines have a lot of benefits, they do carry some harmful effects too. Conclusion
  • 22. 22 Source: •Janes Kuby, 2007, Vaccines, Immunology, W.H. Freeman and Company, Newyork, sixth Edition, Pg. 413- 428. •Satyanarayana U., 2010, Vacines, Biotechnolgy, BOOK’S AND ALLIED (P) Ltd, Kolkata, sixth edition, Pg. 211-212. Net Source: • www.wikipedia.org • www.britannica.com • www.pathmicro.med.sc.edu • www.mpi-magdeburg.mpg.de • www.sc.edu • www.niaid.nih.gov Refere nce