Vaccines provide immunity to diseases and contain agents that stimulate the immune system. There are several types including whole organism vaccines using killed or attenuated microbes, purified components like toxoids and polysaccharides, recombinant and DNA vaccines. Vaccines work by inducing both antibody and cellular immune responses. While effective, they also carry small risks like adverse reactions that researchers continue working to understand and improve safety.
Types of Vaccines with live attenuated, inactivated up to recombination technique. OPV and IPV difference and rationale to replace OPV with IPV. EPI schedule of nepal
Humoral immunity is defined as the immunity mediated by antibodies, which are secreted by B lymphocytes.
B lymphocytes secrete the antibodies into the blood and lymph
Types of Vaccines with live attenuated, inactivated up to recombination technique. OPV and IPV difference and rationale to replace OPV with IPV. EPI schedule of nepal
Humoral immunity is defined as the immunity mediated by antibodies, which are secreted by B lymphocytes.
B lymphocytes secrete the antibodies into the blood and lymph
A vaccine is a biological preparation of weakened or killed pathogen such as bacterium or virus that will improves immunity to a particular diseases.
The principle of immunization or vaccination is based on the property of ‘memory’ of the immune system.
The process of introduction of vaccine into an individual to provide protection against a disease called vaccination.
BP-605T, Pharmaceutical biotechnology, Structure of immunoglobulins, classification of immunoglobulins, explanation of structure of immunoglobulin, digestion with proteolytic enzymes, Fab region, Fc region, role of different immunoglobulin classes, structure of IGM, IGA, IGG, IGE, IGD, Light chain, heavy chain, kappa, lambda, papain enzyme, pepsin enzyme
Immunity is the ability of the body to protect against all types of foreign bodies like bacteria, virus, toxic substances etc. As it protects us from diseases it is also called disease resistance. Immunity is done by immune system which is a complex network of lymphoid organs such as bone marrow, thymus, spleen etc. It is mainly of two types- Natural and Acquired immunity.
antibodies are a large proteins. based on electrophorosis and centrifugation anti bodies are mainly five types .these are protects on human body from various microorganisms.
The word "vaccine" originates from the Latin word “vacca”, meaning “cow” a virus (cowpox) which manly effect the cow. which Edward Jenner demonstrated in 1798 could prevent smallpox in humans.
A Vaccine is a substance that is introduced into the body to stimulate the body’s immune response. (Production of an Antibodies)
Vaccine is an immune biological substance designed to produced specific protection against a disease causing pathogen
It is given to prevent an infectious disease from developing disease in the person.
Vaccines contain the same germs that cause disease. (For example, measles vaccine contains measles virus, and Hib vaccine contains Hib bacteria)
But they have been either killed or weakened to the point that they don’t caused disease.
Objectives:
After the end of the presentation we’ll know -
What is cloning vector?
Why cloning vector?
History
Features of a cloning vector
Types of cloning vector
Plasmid
Bacteriophage
Cosmid
Bacterial Artificial Chromosome (BAC)
Yeast Artificial Chromosome (BAC)
Human Artificial Chromosome (HAC)
Retroviral Vectors
What determines choice of vector?
Vector in molecular gene cloning
Cloning vector - The molecular analysis of DNA has been made possible by the cloning of DNA. The two molecules that are required for cloning are the DNA to be cloned and a cloning vector.
A cloning vector is a small piece of DNA taken from a virus, a plasmid or the cell of a higher organism, that can be stably maintained in an organism and into which a foreign DNA fragment can be inserted for cloning purposes.
Most vectors are genetically engineered.
The cloning vector is chosen according to the size and type of DNA to be cloned.
The vector therefore contains features that allow for the convenient insertion or removal of DNA fragment in or out of the vector, for example by treating the vector and the foreign DNA with a restriction enzyme and then ligating the fragments together.
After a DNA fragment has been cloned into a cloning vector, it may be further subcloned into another vector designed for more specific use.
A vaccine is a biological preparation of weakened or killed pathogen such as bacterium or virus that will improves immunity to a particular diseases.
The principle of immunization or vaccination is based on the property of ‘memory’ of the immune system.
The process of introduction of vaccine into an individual to provide protection against a disease called vaccination.
BP-605T, Pharmaceutical biotechnology, Structure of immunoglobulins, classification of immunoglobulins, explanation of structure of immunoglobulin, digestion with proteolytic enzymes, Fab region, Fc region, role of different immunoglobulin classes, structure of IGM, IGA, IGG, IGE, IGD, Light chain, heavy chain, kappa, lambda, papain enzyme, pepsin enzyme
Immunity is the ability of the body to protect against all types of foreign bodies like bacteria, virus, toxic substances etc. As it protects us from diseases it is also called disease resistance. Immunity is done by immune system which is a complex network of lymphoid organs such as bone marrow, thymus, spleen etc. It is mainly of two types- Natural and Acquired immunity.
antibodies are a large proteins. based on electrophorosis and centrifugation anti bodies are mainly five types .these are protects on human body from various microorganisms.
The word "vaccine" originates from the Latin word “vacca”, meaning “cow” a virus (cowpox) which manly effect the cow. which Edward Jenner demonstrated in 1798 could prevent smallpox in humans.
A Vaccine is a substance that is introduced into the body to stimulate the body’s immune response. (Production of an Antibodies)
Vaccine is an immune biological substance designed to produced specific protection against a disease causing pathogen
It is given to prevent an infectious disease from developing disease in the person.
Vaccines contain the same germs that cause disease. (For example, measles vaccine contains measles virus, and Hib vaccine contains Hib bacteria)
But they have been either killed or weakened to the point that they don’t caused disease.
Objectives:
After the end of the presentation we’ll know -
What is cloning vector?
Why cloning vector?
History
Features of a cloning vector
Types of cloning vector
Plasmid
Bacteriophage
Cosmid
Bacterial Artificial Chromosome (BAC)
Yeast Artificial Chromosome (BAC)
Human Artificial Chromosome (HAC)
Retroviral Vectors
What determines choice of vector?
Vector in molecular gene cloning
Cloning vector - The molecular analysis of DNA has been made possible by the cloning of DNA. The two molecules that are required for cloning are the DNA to be cloned and a cloning vector.
A cloning vector is a small piece of DNA taken from a virus, a plasmid or the cell of a higher organism, that can be stably maintained in an organism and into which a foreign DNA fragment can be inserted for cloning purposes.
Most vectors are genetically engineered.
The cloning vector is chosen according to the size and type of DNA to be cloned.
The vector therefore contains features that allow for the convenient insertion or removal of DNA fragment in or out of the vector, for example by treating the vector and the foreign DNA with a restriction enzyme and then ligating the fragments together.
After a DNA fragment has been cloned into a cloning vector, it may be further subcloned into another vector designed for more specific use.
Complex developmental disability in infancy and early childhood, sign and symptom, its treatment via therapist approaches across the child's daily life
The Biological Plausibility of a Relationship between Vaccines and Autism Spectrum Disorders. A presentation to the Florida Governor's Task Force on Autism Spectrum Disorders
Here in this presentation is proved that vaccines is one of the main causes of autism, mainly due to the presence of mercury in vaccines. Mercury has been known to cause autism.
This presentation discusses about the etiology, clinical features, complications and management of Septic Shock.
The information compiled in this presentation (from medical textbooks and internet sources) does not belong to me, but has been done so for educational purposes
Class switching is the process whereby an activated B cell changes its antibody production from IgM to either IgA, IgG, or IgE depending on the functional requirements. By the end of this CAL you should understand: the basic structure and function of an antibody.
Students, digital devices and success - Andreas Schleicher - 27 May 2024..pptxEduSkills OECD
Andreas Schleicher presents at the OECD webinar ‘Digital devices in schools: detrimental distraction or secret to success?’ on 27 May 2024. The presentation was based on findings from PISA 2022 results and the webinar helped launch the PISA in Focus ‘Managing screen time: How to protect and equip students against distraction’ https://www.oecd-ilibrary.org/education/managing-screen-time_7c225af4-en and the OECD Education Policy Perspective ‘Students, digital devices and success’ can be found here - https://oe.cd/il/5yV
How to Create Map Views in the Odoo 17 ERPCeline George
The map views are useful for providing a geographical representation of data. They allow users to visualize and analyze the data in a more intuitive manner.
Operation “Blue Star” is the only event in the history of Independent India where the state went into war with its own people. Even after about 40 years it is not clear if it was culmination of states anger over people of the region, a political game of power or start of dictatorial chapter in the democratic setup.
The people of Punjab felt alienated from main stream due to denial of their just demands during a long democratic struggle since independence. As it happen all over the word, it led to militant struggle with great loss of lives of military, police and civilian personnel. Killing of Indira Gandhi and massacre of innocent Sikhs in Delhi and other India cities was also associated with this movement.
This is a presentation by Dada Robert in a Your Skill Boost masterclass organised by the Excellence Foundation for South Sudan (EFSS) on Saturday, the 25th and Sunday, the 26th of May 2024.
He discussed the concept of quality improvement, emphasizing its applicability to various aspects of life, including personal, project, and program improvements. He defined quality as doing the right thing at the right time in the right way to achieve the best possible results and discussed the concept of the "gap" between what we know and what we do, and how this gap represents the areas we need to improve. He explained the scientific approach to quality improvement, which involves systematic performance analysis, testing and learning, and implementing change ideas. He also highlighted the importance of client focus and a team approach to quality improvement.
Read| The latest issue of The Challenger is here! We are thrilled to announce that our school paper has qualified for the NATIONAL SCHOOLS PRESS CONFERENCE (NSPC) 2024. Thank you for your unwavering support and trust. Dive into the stories that made us stand out!
2024.06.01 Introducing a competency framework for languag learning materials ...Sandy Millin
http://sandymillin.wordpress.com/iateflwebinar2024
Published classroom materials form the basis of syllabuses, drive teacher professional development, and have a potentially huge influence on learners, teachers and education systems. All teachers also create their own materials, whether a few sentences on a blackboard, a highly-structured fully-realised online course, or anything in between. Despite this, the knowledge and skills needed to create effective language learning materials are rarely part of teacher training, and are mostly learnt by trial and error.
Knowledge and skills frameworks, generally called competency frameworks, for ELT teachers, trainers and managers have existed for a few years now. However, until I created one for my MA dissertation, there wasn’t one drawing together what we need to know and do to be able to effectively produce language learning materials.
This webinar will introduce you to my framework, highlighting the key competencies I identified from my research. It will also show how anybody involved in language teaching (any language, not just English!), teacher training, managing schools or developing language learning materials can benefit from using the framework.
We all have good and bad thoughts from time to time and situation to situation. We are bombarded daily with spiraling thoughts(both negative and positive) creating all-consuming feel , making us difficult to manage with associated suffering. Good thoughts are like our Mob Signal (Positive thought) amidst noise(negative thought) in the atmosphere. Negative thoughts like noise outweigh positive thoughts. These thoughts often create unwanted confusion, trouble, stress and frustration in our mind as well as chaos in our physical world. Negative thoughts are also known as “distorted thinking”.
Ethnobotany and Ethnopharmacology:
Ethnobotany in herbal drug evaluation,
Impact of Ethnobotany in traditional medicine,
New development in herbals,
Bio-prospecting tools for drug discovery,
Role of Ethnopharmacology in drug evaluation,
Reverse Pharmacology.
3. •British physician Edward Jenner, who in 1796 used the cowpox virus (Latin variola
vaccinia) to confer protection against smallpox.
•In 1885 the French microbiologist Louis Pasteur and Emile Roux developed the
first vaccine against rabies.
Introdu
ction
A vaccine is a biological preparation that improves immunity to a particular disease. It
contains certain agents that not only resembles a disease-causing microorganism but it also
stimulates body’s immune sustem recognize the foreign agents.
History:
Definition:
(Ref: www.wikipedia.org, www.britannica.com, www.pathmicro.med.sc.edu)
3
4. 4
Vaccines are dead or inactivated organisms or purified products derived from
them. There are several types of vaccines in use. They are:
Typ
es
• Whole-Organism Vaccines
Killed
Attenuated
• Purified Macromolecules as Vaccines
Toxoids
Capsular polysaccharides
Recombinant microbial antigens/Surface antigens
• Recombinant vaccine
• DNA vaccine
• Multivalent Subunit Vaccines (Ref: Kuby, book for Immunology)
5. 5
Many of the common vaccines currently in use consist of inactivated (killed) or live but
attenuated (avirulent) bacterial cells or viral particles.
Whole-Organism
Vaccines
Killed/Inactivated.
Attenuated.
Killed/ Inactivated: Some vaccines contain killed, but previously virulent, micro-
organisms that have been destroyed with chemicals, heat, radioactivity or antibiotics.
Attenuated: Some vaccines contain live, attenuated microorganisms. Many of these are
live viruses that have been cultivated under conditions that disable their virulent
properties, or which use closely related but less dangerous organisms to produce a broad
immune response.
(Ref: Kuby, book for Immunology)
6. 6
Disease or pathogen Type of vaccine
WHOLE ORGANISMS
Bacterial cells
Anthrax
Cholera
Pertussis*
Plague
Tuberculosis
Typhoid
Inactivated
Inactivated
Inactivated
Inactivated
Live attenuated
Live attenuated
Classification of common
vaccines for humans:
(Ref: Kuby, book for Immunology)
7. 7
Disease or pathogen Type of vaccine
Viral particles
Hepatitis A
Influenza
Measles
Polio (Sabin)
Polio (Salk)
Rabies
Rotavirus
Varicella zoster (chickenpox)
Yellow fever
Inactivated
Inactivated
Live attenuated
Live attenuated
Inactivated
Inactivated
Live attenuated
Live attenuated
Live attenuated
(Ref: Kuby, book for Immunology)
8. 8
• Inactivated exotoxins.
• Capsular polysaccharides.
• Recombinant microbial antigens/Surface antigens.
Purified Macromolecules as Vaccine
(Ref: Kuby, Book for Immunology)
9. 9
Inactivated
exotoxins/Toxoid
• Toxoids are vaccines which consist of exotoxins that have been inactivated,
either by heat or chemicals. These vaccines are intended to build immunity
against the toxins, but not necessarily the bacteria that produce the toxins.
• Some examples are botulinum antitoxin and diphtheria antitoxin.
Fig: Modification of toxin to toxoid
(Ref: Kuby, www2a.cdc.gov)
10. 10
• The virulence of some pathogenic bacteria depends primarily on the anti
phagocytic properties of their hydrophilic polysaccharide capsule.
• Coating of the capsule with antibodies and or complement greatly increases
the ability of macrophages and neutrophils to phagocytose such pathogens.
• The current vaccine for Streptococcus pneumoniae, which causes
pneumococcal pneumonia, consists of 23 antigenically different capsular
polysaccharides.
Capsular polysaccharides
(Ref: Kuby, www2a.cdc.gov)
11. 11
• The gene encoding any immunogenic protein can be cloned and expressed
in bacterial, yeast, or mammalian cells using recombinant DNA technology.
• The first such recombinant antigen vaccine approved for human use is the
hepatitis B vaccine. This vaccine was developed by cloning the gene for the
major surface antigen of hepatitis B virus (HBsAg) and expressing it in yeast
cells.
Recombinant microbial
antigens/Surface antigen
(Ref: Kuby, book for Immunology)
12. 12
(Ref: Kuby, book for Immunology)
Recombinant Vaccines:
Fig: Production of vaccinia vector vaccine.
15. 15
(Ref: Kuby, book for Immunology)
Solid matrix–antibody-antigen
(SMAA) complex
Fig: Solid matrix Antigen
16. 16
(Ref: Kuby, book for Immunology
Detergent to protein antigens
Fig: b. Detergent extracted membrane antigens or antigenic peptides
c. ISCOM delivery of antigen into cell
17. 17
Mechanism of a vaccine
Fig: Mechanism of vaccine
(Ref: www.pathmicro.med.sc.edu)
18. 18
Vaccines do not guarantee complete protection from a disease.
Adjuvants:
•An adjuvant (Latin, adiuvare: to aid) is a pharmacological or immunological
agent that modifies the effect of other agents, such as a drug or vaccine. They
are often included in vaccines to enhance the recipient's immune response to a
supplied antigen, while keeping the injected foreign material to a minimum.
Effectiv
eness
(Ref: www.wikipedia.org)
19. 19
• The primary risk associated with vaccines, especially vaccines that utilize live
organisms, is that the vaccine itself causes illness.
•Another risk is that the vaccine may behave as a super antigen and over stimulate the
immune system.
•Yet a third risk is that some individuals may have an allergic reaction to the vaccine,
especially vaccines produced in Embryonated chicken eggs and in transgenic plants.
Risks associated
with vaccinesVaccines also have some sort of risks, like:
(Ref: www.wikipedia.org)
20. 20
• Approaches for designing a preventive HIV vaccine.
• Vaccine against Dengue Vaccine.
• NIH Scientists Identify New HIV-Inhibiting Protein.
•NIH Scientists Find Cause of Rare Immune Disease: Genetic Mutation Leads to Cold
Allergy, Immune Deficiency and Autoimmunity.
• NIH Found a Gene That May Play a Role in Type 1 Diabetes.
Recent Research:
(Ref: www.niaid.nih.gov)
21. 21
Vaccines are one of the most effective health interventions
ever developed. Three types of vaccines are currently used in humans: attenuated
(avirulent) microorganisms, inactivated (killed) microorganisms, or purified
macromolecules. Recombinant vector vaccine and Plasmid DNA vaccines are also used.
They induce both humoral and cell-mediated immunity. Some boosters (called adjuvants)
are also used in association with vaccines for increasing the immune response. As the
vaccines have a lot of benefits, they do carry some harmful effects too.
Conclusion