DNA vaccines work by injecting DNA encoding antigens from pathogens. The host cells use this DNA to produce antigens, which are then displayed on the cell surface and trigger both humoral and cellular immune responses. DNA vaccines offer advantages over traditional vaccines like avoiding infectious organisms, not requiring refrigeration, and stimulating both arms of the immune system. They have shown protection against diseases in animal studies and have potential applications for influenza, hepatitis B, HIV, and malaria vaccines. However, DNA vaccines also have disadvantages like weak immune responses in humans.

1. Presented by-
Rahima Akhter Nury: 132 1722 046
Suraiya Ahmed: 132 0916 046
Anik khan: 132 0430 046
MD Isfat Hasan Tomal: 132 0378 046
Ramisha Rifat Tony: 132 0332 646

2. DNA Vaccine
production

3. What is DNA
vaccine?
• DNA vaccines are the vaccines
which contain DNA that codes for
specific proteins (antigens) from a
pathogen.
• The DNA is injected into cells
• uses the DNA to synthesize the
proteins
• Because these proteins are
recognized as foreign, when they are
processed by the host cells and
displayed on their surface, the
immune system is alerted,
• which then triggers immune
responses.

4. Why DNA vaccines?
This approach offers a number of potential
advantages over traditional approaches
Uses only the DNA from
infectious organisms.
Avoid the risk of using actual
infectious organism.
Provide both Humoral & Cell
mediated immunity
Refrigeration is not required
DNA vaccines Traditional vaccines
Uses weakened or killed form
of infectious organism.
Create possible risk of the
vaccine being fatal.
Provide primarily Humoral
immunity
Usually requires Refrigeration.

5. Including :
• the stimulation of both B- and T-cell
responses,
• improved vaccine stability,
• the absence of any infectious agent
• the relative ease of large-scale manufacture.
• immune responses in animals have been
obtained using genes from a variety of
infectious agents, including influenza virus,
hepatitis B virus, human immunodeficiency
virus, rabies virus, malarial parasites etc.
• In some cases, protection from disease in
animals has also been obtained.

6. Viral gene
Recombinant DNA
Technology
Expression plasmid
Plasmid with foreign gene
Principal of DNA Vaccine?

7. Transform in to bacteria
Plasmid DNA get Amplified

8. Plasmid DNA isolated
Stored in vials
Ready for Apply

10. Pneumatic Jet Injection: Very high amount
of vaccine applied to the abdominal skin.

11. How DNA vaccines work??
By 2 Pathway :
1. Endogenous pathway 2. Exogenous pathway

12. Production of DNA
vaccine

14. ADVANTAGES

15. DISADVANTAGES

16. APPLICATIONS

17. CONCLUSION

18. REFERENCES
1. Wolff JA, Malone RW, Williams P, et al. Direct gene
transfer into mouse muscle in
vivo. Science.1990;247(4949 Pt 1):1465–8. [PubMed]
2. Klinman DM, Yamshchikov G, Ishigatsubo Y. Contribution
of CpG motifs to the immunogenicity of DNA vaccines. J
Immunol. 1997;158(8):3635–9. [PubMed]
3. Khan KH. Vectors Used in Gene Manipulation – A
retrospective. Advanced Biotech Journal - Online -
Tutorial review. 2009;9(2):1–8
4. Corr M, Lee DJ, Carson DA, et al. Gene vaccination with
naked plasmid DNA: mechanism of CTL priming. J Exp
Med. 1996;184(4):1555–60. [PMC free article] [PubMed]
5. http://www.mdpi.com/2076-393X/1/3/225/htm