This document provides an overview of DNA microarray technology. It discusses that a DNA microarray contains DNA spots attached to a solid surface with specific DNA sequences known as probes. Each probe occupies a spot on the chip and fluorescent activity from labeled target sequences binding to probes allows measurement of gene expression levels. The document outlines the historical development of microarrays and describes the basic principles and process, including sample preparation, hybridization of labeled targets to probes, washing, and image analysis. It distinguishes between cDNA and oligonucleotide microarrays and lists requirements for a microarray system.
Microarray -types, DNA chip, Principle and application of microarray, Preparation of DNA Chip, Affymetrix chip, microarray in genomics and proteomics, advantages and limitations of microarray
Microarray -types, DNA chip, Principle and application of microarray, Preparation of DNA Chip, Affymetrix chip, microarray in genomics and proteomics, advantages and limitations of microarray
The above presentation consist of the definition of microarray, brief history, general principle of the same, the type of scanner that are used to read or to scan the microarray , type of DNA microarray and finally its various apliccation including the role of DNA microaarray in drug discovery.
pBluescript is an example of a combination between plasmids and phages (phagemids).
Phagemids represent a hybrid type of class of vectors that serve to produce single-stranded DNA.
2D-PAGE is a method is used for the separation and identification of proteins in a complex mixture using two separate dimensions that are run perpendicular to one another.
2D-DIGE is an advanced version of classical two-dimensional gel electrophoresis (2D-PAGE).
The protein samples are labeled with fluorescent dyes and then separated by 2D-PAGE.
INTRODUCTION TO REAL TIME PCR IS GIVEN, basic principle of realtime pcr, along with the process of operating this, diagrammatic representation of the process, advantages and disadvantages o f reatimem pcr, applications of the same is also there
Hello There,
DNA Footprinting Is A Molecular Biology Technique With Wide Applications In Many Areas Of Biological Sciences And Importantly It Is Used For Crime Detection In Forensic Sciences. In This Presentation, You Will Learn What It Is, The Technology, Protocol, Pictorial Representation, Applications And References For Further Study.
Applications of genomics and proteomics pptIbad khan
Applications of genomics and proteomics ppt
genomics and proteomics ppt
in the field of health genomics and proteomics ppt
oncology ppt
biomedical application of genomics and proteomics ppt
agriculture application of genomics and proteomics ppt
proteomics in agriculture ppt
diagnosis of infectious disease ppt
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Genomic sequencing a sub-disciplinary branch of genetics and difference between the two sequencers used to sequence the genome basically automated sequencer and fluorescence sequencers and its applications.
The above presentation consist of the definition of microarray, brief history, general principle of the same, the type of scanner that are used to read or to scan the microarray , type of DNA microarray and finally its various apliccation including the role of DNA microaarray in drug discovery.
pBluescript is an example of a combination between plasmids and phages (phagemids).
Phagemids represent a hybrid type of class of vectors that serve to produce single-stranded DNA.
2D-PAGE is a method is used for the separation and identification of proteins in a complex mixture using two separate dimensions that are run perpendicular to one another.
2D-DIGE is an advanced version of classical two-dimensional gel electrophoresis (2D-PAGE).
The protein samples are labeled with fluorescent dyes and then separated by 2D-PAGE.
INTRODUCTION TO REAL TIME PCR IS GIVEN, basic principle of realtime pcr, along with the process of operating this, diagrammatic representation of the process, advantages and disadvantages o f reatimem pcr, applications of the same is also there
Hello There,
DNA Footprinting Is A Molecular Biology Technique With Wide Applications In Many Areas Of Biological Sciences And Importantly It Is Used For Crime Detection In Forensic Sciences. In This Presentation, You Will Learn What It Is, The Technology, Protocol, Pictorial Representation, Applications And References For Further Study.
Applications of genomics and proteomics pptIbad khan
Applications of genomics and proteomics ppt
genomics and proteomics ppt
in the field of health genomics and proteomics ppt
oncology ppt
biomedical application of genomics and proteomics ppt
agriculture application of genomics and proteomics ppt
proteomics in agriculture ppt
diagnosis of infectious disease ppt
personalized medicine ppt
Genomic sequencing a sub-disciplinary branch of genetics and difference between the two sequencers used to sequence the genome basically automated sequencer and fluorescence sequencers and its applications.
In biology, cloning is the process of producing similar populations of genetically identical individuals that occurs in nature when organisms such as bacteria, insects or plants reproduce asexually. Cloning in biotechnology refers to processes used to create copies of DNA fragments (molecular cloning), cells (cell cloning), or organisms. The term also refers to the production of multiple copies of a product such as digital media or software.
This presentation was given to me during my higher education in the Lebanese University, Faculty of Education. It includes detailed explanation about DNA microarray.
The DNA microarray is a tool used to determine whether the DNA from a particular individual contains a mutation in genes like BRCA1 and BRCA2. The chip consists of a small glass plate encased in plastic. Some companies manufacture microarrays using methods similar to those used to make computer microchips.
A DNA microarray is a collection of microscopic DNA spots attached to a solid surface. Scientists use DNA microarrays to measure the expression levels of large numbers of genes simultaneously or to genotype multiple regions of a genome. Each DNA spot contains picomoles of a specific DNA sequence, known as probes.
This chapter provides an overview of DNA microarrays. Microarrays are a technology in which 1000’s of nucleic acids are bound to a surface and are used to measure the relative concentration of nucleic acid sequences in a mixture via hybridization and subsequent detection of the hybridization events. We first cover the history of microarrays and the antecedent technologies that led to their development. We then discuss the methods of manufacture of microarrays and the most common biological applications. The chapter ends with a brief discussion of the limitations of microarrays and discusses how microarrays are being rapidly replaced by DNA sequencing technologies.
The DNA microarray is a tool used to determine whether the DNA from a particular individual contains a mutation in genes like BRCA1 and BRCA2. The chip consists of a small glass plate encased in plastic. Some companies manufacture microarrays using methods similar to those used to make computer microchips.
Honest Reviews of Tim Han LMA Course Program.pptxtimhan337
Personal development courses are widely available today, with each one promising life-changing outcomes. Tim Han’s Life Mastery Achievers (LMA) Course has drawn a lot of interest. In addition to offering my frank assessment of Success Insider’s LMA Course, this piece examines the course’s effects via a variety of Tim Han LMA course reviews and Success Insider comments.
How to Make a Field invisible in Odoo 17Celine George
It is possible to hide or invisible some fields in odoo. Commonly using “invisible” attribute in the field definition to invisible the fields. This slide will show how to make a field invisible in odoo 17.
2024.06.01 Introducing a competency framework for languag learning materials ...Sandy Millin
http://sandymillin.wordpress.com/iateflwebinar2024
Published classroom materials form the basis of syllabuses, drive teacher professional development, and have a potentially huge influence on learners, teachers and education systems. All teachers also create their own materials, whether a few sentences on a blackboard, a highly-structured fully-realised online course, or anything in between. Despite this, the knowledge and skills needed to create effective language learning materials are rarely part of teacher training, and are mostly learnt by trial and error.
Knowledge and skills frameworks, generally called competency frameworks, for ELT teachers, trainers and managers have existed for a few years now. However, until I created one for my MA dissertation, there wasn’t one drawing together what we need to know and do to be able to effectively produce language learning materials.
This webinar will introduce you to my framework, highlighting the key competencies I identified from my research. It will also show how anybody involved in language teaching (any language, not just English!), teacher training, managing schools or developing language learning materials can benefit from using the framework.
Safalta Digital marketing institute in Noida, provide complete applications that encompass a huge range of virtual advertising and marketing additives, which includes search engine optimization, virtual communication advertising, pay-per-click on marketing, content material advertising, internet analytics, and greater. These university courses are designed for students who possess a comprehensive understanding of virtual marketing strategies and attributes.Safalta Digital Marketing Institute in Noida is a first choice for young individuals or students who are looking to start their careers in the field of digital advertising. The institute gives specialized courses designed and certification.
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Read| The latest issue of The Challenger is here! We are thrilled to announce that our school paper has qualified for the NATIONAL SCHOOLS PRESS CONFERENCE (NSPC) 2024. Thank you for your unwavering support and trust. Dive into the stories that made us stand out!
Acetabularia Information For Class 9 .docxvaibhavrinwa19
Acetabularia acetabulum is a single-celled green alga that in its vegetative state is morphologically differentiated into a basal rhizoid and an axially elongated stalk, which bears whorls of branching hairs. The single diploid nucleus resides in the rhizoid.
Macroeconomics- Movie Location
This will be used as part of your Personal Professional Portfolio once graded.
Objective:
Prepare a presentation or a paper using research, basic comparative analysis, data organization and application of economic information. You will make an informed assessment of an economic climate outside of the United States to accomplish an entertainment industry objective.
A Strategic Approach: GenAI in EducationPeter Windle
Artificial Intelligence (AI) technologies such as Generative AI, Image Generators and Large Language Models have had a dramatic impact on teaching, learning and assessment over the past 18 months. The most immediate threat AI posed was to Academic Integrity with Higher Education Institutes (HEIs) focusing their efforts on combating the use of GenAI in assessment. Guidelines were developed for staff and students, policies put in place too. Innovative educators have forged paths in the use of Generative AI for teaching, learning and assessments leading to pockets of transformation springing up across HEIs, often with little or no top-down guidance, support or direction.
This Gasta posits a strategic approach to integrating AI into HEIs to prepare staff, students and the curriculum for an evolving world and workplace. We will highlight the advantages of working with these technologies beyond the realm of teaching, learning and assessment by considering prompt engineering skills, industry impact, curriculum changes, and the need for staff upskilling. In contrast, not engaging strategically with Generative AI poses risks, including falling behind peers, missed opportunities and failing to ensure our graduates remain employable. The rapid evolution of AI technologies necessitates a proactive and strategic approach if we are to remain relevant.
4. A DNA microarray (also commonly known as DNA Chip or biochip) is a
collection of microscopic DNA spots attached to a solid surface.
Each DNA spot contains picomoles (10−12 moles) of a specific DNA sequence,
known as probes (or oligos).
Each known gene or “probe” occupies a particular “spot” on the chip, and varying
levels of fluorescent activity show varying levels of gene activity in introduced genetic
material.
Fluorescently labeled target sequences that bind to a probe sequence generate a
signal.
5. Historical background : Evolved from southern blotting
The concept of DNA microarrays began in the mid 1980s.
• Pin based robotic system was developed by Lehrach’s group in 1990.
•“Quantitative Monitoring of Gene Expression Patterns with a complementary
DNA microarray” reported by Patrick Brown, Mark Schena and colleagues in
Science (1995).
• Steve Fodor developed scanner for reading the output.
• Mark schena was proclaimed as the “Father of Microarray
Technology”.
Steve Fodor
Mark schenaPatrick Brown
•
•
•
6. • 1991 - Photolithographic printing (Affymetrix)
• 1994 - First cDNA collections are developed at Stanford.
• 1996 - Commercialization of arrays (Affymetrix)
• 1997- Genome-wide expression monitoring in S. cerevisiae (yeast)
• 2003 - Introduction to clinical practice
• 2004-Whole human genome on one microarray
Singh, A., & Kumar, N. (2013). A review on DNA microarray Technology. International
Journal of Current Research and Review, 5(22), 01-05.
7. The core principle behind microarrays is
hybridization.
Samples are labelled using fluorescent dyes.
At least two samples are hybridized to chip.
Complementary nucleic acid sequences
get pair via hydrogen bonds.
Washing off of non-specific bonding
sequences .
Sample
Preparation and
labelling
Hybridisation Washing
Image acquisition
and data analysis
8. There are 2 types of DNA Chips/Microarrays:
1. cDNA based microarray (or) Spotted DNA arrays
2. Oligonucleotide based microarray
9. There are certain requirements for designing a
DNA microarray system viz.,:
1. DNA Chip
2. Target sample (Fluorescently labelled)
3. Fluorescent dyes
4. Probes
5. Scanner
10. Various types of cells in body are different from one another due to different genes being
“turned on” and “turned off”. Because of differ in GENE EXPRESSION
2. Tissue samples dissolved in organic solvent .RNA extracted.
DNA, proteins and other cell components separated.
1. Sample extraction from healthy and unhealthy tissue of same
plant.
Healthy Unhealthy
Mixing the tissue samples on the Vortex. RNA will be
released. Samples are centrifuged to separate RNA
from rest of the cell components
11. • After centrifugation, RNA separated from the rest.
Then both samples are pipetted out in different
collection tubes
• RNA Samples washed over Columns filled
with small beads
• These beads bind only to RNA strands with
Poly-A tail
• Other molecules washed away
Healthy Unhealthy
Healthy Unhealthy
Healthy Unhealthy
• Wash Buffer solution over beads
• mRNA detached from the beads
Healthy Unhealthy
• DNA copy of the RNA is made giving it
some colour as labelling mix
• Green--Healthy cells RNA
• Red - infected cells RNA
12. • Reverse transcriptase assembles
labelled nucleotides into a cDNA
molecule
• mRNA molecule degraded.
Both the Samples are spread on the Microarray
13. • Microarray placed in washing solution. Extra cDNA
present on slide are washed off easily as it won’t bind.
HEALTHY CELLS
• Many of the spots are Green but not every
• Dark Spots are genes not transcribed in Healthy cells
CANCER CELLS
• Red Spots pattern looks different
• Infected cells and Healthy skin cells have different
gene expression pattern
RED AND GREEN SPOTS MERGED TOGETHER
• Yellow spot –gene expressed both in healthy cells and unhealthy cells
• Yellow genes—No change in activity when cell becomes unhealthy
• Red spots--genes “turnedon” in unhealthy cells
producing more mRNA
• Green spots –genes “turned-off’ in unhealthy cells
14. Microarrays use relative quantization in which intensity if spot is compared to the same spot
Under a different condition which is known by its position
• The two samples to be compared (pair wise
comparison) are grown/acquired.
• RNA, DNA, DNA/RNA bound to a protein
• Optional PCR Amplification
• The label is added either in the RT step or in an
additional step after amplification if present.
• This mix is denatured and added to a pin hole in a
microarray.
• The holes are sealed and the microarray hybridized.
• The microarray is dried and scanned in a special
machine where a laser excites the dye and a detector
measures its emission. After that the raw that is
normalized for study
15.
16. Gene Chips
1. Oligonucleotide arrays (Affymetrix)
Small number of 20-25mers/gene
Enabled by photolithography from the computer industry
Off the shelf
2. Ink-jet microarrays (Agilent)
Large number of 25-60mers “printed” directly on glass
Four cartridges: A, C, G, and T
Flexible, rapid, but expensive.
17. Spotted Array Affey gene chips
Relative cheap to make (~$10 slide) Expensive ($500 or more)
Flexible - spot anything you want Limited types available, no chance
of specialized chips
Cheap so can repeat
experiments many times
Fewer repeated experiments
usually
Highly variable spot
deposition
More uniform DNA features
Usually have to make your
own
Can buy off the shelf
Editor's Notes
. The purified RNA is analysed for quality (by capillary
electrophoresis) and quantity (by using a nanodrop spectrometer)
The labeled samples are then mixed with a propriety
hybridization solution. SDS, SSC, dextran sulfate, a
blocking agent, Denhardt's solution and formamine.