This document discusses leprosy, a chronic disease caused by Mycobacterium leprae. Key points include:
- Leprosy affects the skin and nerves and can cause deformities if left untreated. It is transmitted through droplets.
- M. leprae is an acid-fast bacillus that is intracellular and can be seen in bundles or "globi" inside cells.
- Leprosy manifestations depend on host cell-mediated immunity and range from tuberculoid leprosy with good immunity to lepromatous leprosy with poor immunity.
- Diagnosis involves examination of skin and nasal smears for acid-fast bacilli as well as le
The PPT is mainly all about Mycobacterium Tuberculosis. Agents causing the disease Tuberculosis, pathogenesis, laboratory diagnosis, treatment and prophylaxis. It was made for both BSc and MSc students.
The PPT is mainly all about Mycobacterium Tuberculosis. Agents causing the disease Tuberculosis, pathogenesis, laboratory diagnosis, treatment and prophylaxis. It was made for both BSc and MSc students.
Introduction
Disease
Important Properties
Transmission & Epidemiology
Risk factor of reactivation
Pathogenesis
Clinical Findings
Laboratory Diagnosis
Approaches to the diagnosis of latent infections
Treatment
Prevention
pseudomonas aeruginosa is one of the leading cause of hospital-associated infection. mainly Pseudomonas is a multi drug resistant bacteria.
they are oxidase positive, non fermenters, strictly aerobic bacteria.
they are pigment producing, pigment can be appreciated on nutrient agar.
Cryptococcosis also called as Torulosis is a subacute or chronic fungal infection caused by Cryptococcus neoformans. It leads to compications such as fatal meningoencephalitis. It is an opportunistic infection in HIV-infected patients. The PPT discuss on the morphology of the fungus, pathogenesis, laboratory diagnosis and treatment.
Cholera is a serious bacterial disease that usually
causes severe diarrhea and dehydration. The disease is typically spread through contaminated water.
Modern sewage and water treatment have effectively eliminated cholera in most countries. It’s still a problem in countries like Asia, America and Africa. Mostly in India.
Countries affected by war, poverty, and natural disasters have the greatest risk for a cholera outbreak.
Taxonomy:
class : Gamma Proteobacteria
Order: Vibrionales
Family: Vibrionaceae
Genus: Vibrio
Species: v.cholerae, v.parahaemolyticus,
v. vulnificus, v. alginolyticus
MORPHOLOGY:
Gram negative, actively motile, short, rigid curved bacilli
Resembling letter “V”
about 34 genus
most common in water
1.5µ X 0.2 -0.4 µ in size
polar flagellum , strongly aerobic
Smear – fish in stream appearance
PATHOGENESIS:
Source: Ingestion of contaminated water, food,
fruits and vegetables etc.,
Incubation periods: 1-5 days
Symptoms: Watery diarrhoea, vomiting, thirst, dehydration, muscle cramps
Complications: muscular pain, renal failure, pulmonary edema, cardiac arrhythrnias
DIAGNOSIS:
Specimen: stool sample, water sample(envt)
Microscopy: a) Hanging drop : +ve
b) Gram stain :-ve
Culture: Mac conkey Agar :colourless to light pink
TCBS : yellow colonies
Serology: serological tests are no diagnostic value
TREATMENT:
Adequate replacement of fluids and electrolytes.
Oral tetracycline reduces the period of vibrio excreation.
PREVENTION:
Drink and use bottled water
Frequent washing
Sanitary environment
Defecate in water
Cook food thoroughly
Mycobacterium is a genus of Actinobacteria, given its own family, the Mycobacteriaceae. Over 190 species are recognized in this genus. This genus includes pathogens known to cause serious diseases in mammals, including tuberculosis (Mycobacterium tuberculosis) and leprosy (Mycobacterium leprae) in humans.
Introduction
Disease
Important Properties
Transmission & Epidemiology
Risk factor of reactivation
Pathogenesis
Clinical Findings
Laboratory Diagnosis
Approaches to the diagnosis of latent infections
Treatment
Prevention
pseudomonas aeruginosa is one of the leading cause of hospital-associated infection. mainly Pseudomonas is a multi drug resistant bacteria.
they are oxidase positive, non fermenters, strictly aerobic bacteria.
they are pigment producing, pigment can be appreciated on nutrient agar.
Cryptococcosis also called as Torulosis is a subacute or chronic fungal infection caused by Cryptococcus neoformans. It leads to compications such as fatal meningoencephalitis. It is an opportunistic infection in HIV-infected patients. The PPT discuss on the morphology of the fungus, pathogenesis, laboratory diagnosis and treatment.
Cholera is a serious bacterial disease that usually
causes severe diarrhea and dehydration. The disease is typically spread through contaminated water.
Modern sewage and water treatment have effectively eliminated cholera in most countries. It’s still a problem in countries like Asia, America and Africa. Mostly in India.
Countries affected by war, poverty, and natural disasters have the greatest risk for a cholera outbreak.
Taxonomy:
class : Gamma Proteobacteria
Order: Vibrionales
Family: Vibrionaceae
Genus: Vibrio
Species: v.cholerae, v.parahaemolyticus,
v. vulnificus, v. alginolyticus
MORPHOLOGY:
Gram negative, actively motile, short, rigid curved bacilli
Resembling letter “V”
about 34 genus
most common in water
1.5µ X 0.2 -0.4 µ in size
polar flagellum , strongly aerobic
Smear – fish in stream appearance
PATHOGENESIS:
Source: Ingestion of contaminated water, food,
fruits and vegetables etc.,
Incubation periods: 1-5 days
Symptoms: Watery diarrhoea, vomiting, thirst, dehydration, muscle cramps
Complications: muscular pain, renal failure, pulmonary edema, cardiac arrhythrnias
DIAGNOSIS:
Specimen: stool sample, water sample(envt)
Microscopy: a) Hanging drop : +ve
b) Gram stain :-ve
Culture: Mac conkey Agar :colourless to light pink
TCBS : yellow colonies
Serology: serological tests are no diagnostic value
TREATMENT:
Adequate replacement of fluids and electrolytes.
Oral tetracycline reduces the period of vibrio excreation.
PREVENTION:
Drink and use bottled water
Frequent washing
Sanitary environment
Defecate in water
Cook food thoroughly
Mycobacterium is a genus of Actinobacteria, given its own family, the Mycobacteriaceae. Over 190 species are recognized in this genus. This genus includes pathogens known to cause serious diseases in mammals, including tuberculosis (Mycobacterium tuberculosis) and leprosy (Mycobacterium leprae) in humans.
Aerobic Non-Spore-Forming Gram-Positive BacilliSijo A
Disease: listeriosis.
L. monocytogenes causes a variety of infections in neonates, pregnant women, and immunosuppressed patients.
CNS infections: meningitis, encephalitis, brain abscess, spinal cord infections.
Neonatal:
Early onset: Granulomatosis infantisepticum—in utero infection disseminated systemically that causes stillbirth.
Late onset: Bacterial meningitis.
Food poisoning, bacteremia.
Mode of transmission:
Direct contact: Human gastrointestinal tract, ingestion of contaminated food, such as meat and dairy products.
Endogenous strain: Colonized mothers may pass organism to fetus. Portal of entry is probably from gastrointestinal tract to blood and in some instances from blood to meninges.
The following presentation is only for quick reference. I would advise you to read the theoretical aspects of the respective topic and then use this presentation for your last minute revision. I hope it helps you..!!
Mayur D. Chauhan
Visceral leishmaniasis (VL), also known as kala-azar, is the most severe form of leishmaniasis caused by the protozoan parasite Leishmania donovani and transmitted by the infected sandflies. It characterized by irregular bouts of fever, substantial weight loss, swelling of the spleen and liver, and anaemia.
Recomendações da OMS sobre cuidados maternos e neonatais para uma experiência pós-natal positiva.
Em consonância com os ODS – Objetivos do Desenvolvimento Sustentável e a Estratégia Global para a Saúde das Mulheres, Crianças e Adolescentes, e aplicando uma abordagem baseada nos direitos humanos, os esforços de cuidados pós-natais devem expandir-se para além da cobertura e da simples sobrevivência, de modo a incluir cuidados de qualidade.
Estas diretrizes visam melhorar a qualidade dos cuidados pós-natais essenciais e de rotina prestados às mulheres e aos recém-nascidos, com o objetivo final de melhorar a saúde e o bem-estar materno e neonatal.
Uma “experiência pós-natal positiva” é um resultado importante para todas as mulheres que dão à luz e para os seus recém-nascidos, estabelecendo as bases para a melhoria da saúde e do bem-estar a curto e longo prazo. Uma experiência pós-natal positiva é definida como aquela em que as mulheres, pessoas que gestam, os recém-nascidos, os casais, os pais, os cuidadores e as famílias recebem informação consistente, garantia e apoio de profissionais de saúde motivados; e onde um sistema de saúde flexível e com recursos reconheça as necessidades das mulheres e dos bebês e respeite o seu contexto cultural.
Estas diretrizes consolidadas apresentam algumas recomendações novas e já bem fundamentadas sobre cuidados pós-natais de rotina para mulheres e neonatos que recebem cuidados no pós-parto em unidades de saúde ou na comunidade, independentemente dos recursos disponíveis.
É fornecido um conjunto abrangente de recomendações para cuidados durante o período puerperal, com ênfase nos cuidados essenciais que todas as mulheres e recém-nascidos devem receber, e com a devida atenção à qualidade dos cuidados; isto é, a entrega e a experiência do cuidado recebido. Estas diretrizes atualizam e ampliam as recomendações da OMS de 2014 sobre cuidados pós-natais da mãe e do recém-nascido e complementam as atuais diretrizes da OMS sobre a gestão de complicações pós-natais.
O estabelecimento da amamentação e o manejo das principais intercorrências é contemplada.
Recomendamos muito.
Vamos discutir essas recomendações no nosso curso de pós-graduação em Aleitamento no Instituto Ciclos.
Esta publicação só está disponível em inglês até o momento.
Prof. Marcus Renato de Carvalho
www.agostodourado.com
Knee anatomy and clinical tests 2024.pdfvimalpl1234
This includes all relevant anatomy and clinical tests compiled from standard textbooks, Campbell,netter etc..It is comprehensive and best suited for orthopaedicians and orthopaedic residents.
- Video recording of this lecture in English language: https://youtu.be/lK81BzxMqdo
- Video recording of this lecture in Arabic language: https://youtu.be/Ve4P0COk9OI
- Link to download the book free: https://nephrotube.blogspot.com/p/nephrotube-nephrology-books.html
- Link to NephroTube website: www.NephroTube.com
- Link to NephroTube social media accounts: https://nephrotube.blogspot.com/p/join-nephrotube-on-social-media.html
Flu Vaccine Alert in Bangalore Karnatakaaddon Scans
As flu season approaches, health officials in Bangalore, Karnataka, are urging residents to get their flu vaccinations. The seasonal flu, while common, can lead to severe health complications, particularly for vulnerable populations such as young children, the elderly, and those with underlying health conditions.
Dr. Vidisha Kumari, a leading epidemiologist in Bangalore, emphasizes the importance of getting vaccinated. "The flu vaccine is our best defense against the influenza virus. It not only protects individuals but also helps prevent the spread of the virus in our communities," he says.
This year, the flu season is expected to coincide with a potential increase in other respiratory illnesses. The Karnataka Health Department has launched an awareness campaign highlighting the significance of flu vaccinations. They have set up multiple vaccination centers across Bangalore, making it convenient for residents to receive their shots.
To encourage widespread vaccination, the government is also collaborating with local schools, workplaces, and community centers to facilitate vaccination drives. Special attention is being given to ensuring that the vaccine is accessible to all, including marginalized communities who may have limited access to healthcare.
Residents are reminded that the flu vaccine is safe and effective. Common side effects are mild and may include soreness at the injection site, mild fever, or muscle aches. These side effects are generally short-lived and far less severe than the flu itself.
Healthcare providers are also stressing the importance of continuing COVID-19 precautions. Wearing masks, practicing good hand hygiene, and maintaining social distancing are still crucial, especially in crowded places.
Protect yourself and your loved ones by getting vaccinated. Together, we can help keep Bangalore healthy and safe this flu season. For more information on vaccination centers and schedules, residents can visit the Karnataka Health Department’s official website or follow their social media pages.
Stay informed, stay safe, and get your flu shot today!
New Drug Discovery and Development .....NEHA GUPTA
The "New Drug Discovery and Development" process involves the identification, design, testing, and manufacturing of novel pharmaceutical compounds with the aim of introducing new and improved treatments for various medical conditions. This comprehensive endeavor encompasses various stages, including target identification, preclinical studies, clinical trials, regulatory approval, and post-market surveillance. It involves multidisciplinary collaboration among scientists, researchers, clinicians, regulatory experts, and pharmaceutical companies to bring innovative therapies to market and address unmet medical needs.
Pulmonary Thromboembolism - etilogy, types, medical- Surgical and nursing man...VarunMahajani
Disruption of blood supply to lung alveoli due to blockage of one or more pulmonary blood vessels is called as Pulmonary thromboembolism. In this presentation we will discuss its causes, types and its management in depth.
Title: Sense of Smell
Presenter: Dr. Faiza, Assistant Professor of Physiology
Qualifications:
MBBS (Best Graduate, AIMC Lahore)
FCPS Physiology
ICMT, CHPE, DHPE (STMU)
MPH (GC University, Faisalabad)
MBA (Virtual University of Pakistan)
Learning Objectives:
Describe the primary categories of smells and the concept of odor blindness.
Explain the structure and location of the olfactory membrane and mucosa, including the types and roles of cells involved in olfaction.
Describe the pathway and mechanisms of olfactory signal transmission from the olfactory receptors to the brain.
Illustrate the biochemical cascade triggered by odorant binding to olfactory receptors, including the role of G-proteins and second messengers in generating an action potential.
Identify different types of olfactory disorders such as anosmia, hyposmia, hyperosmia, and dysosmia, including their potential causes.
Key Topics:
Olfactory Genes:
3% of the human genome accounts for olfactory genes.
400 genes for odorant receptors.
Olfactory Membrane:
Located in the superior part of the nasal cavity.
Medially: Folds downward along the superior septum.
Laterally: Folds over the superior turbinate and upper surface of the middle turbinate.
Total surface area: 5-10 square centimeters.
Olfactory Mucosa:
Olfactory Cells: Bipolar nerve cells derived from the CNS (100 million), with 4-25 olfactory cilia per cell.
Sustentacular Cells: Produce mucus and maintain ionic and molecular environment.
Basal Cells: Replace worn-out olfactory cells with an average lifespan of 1-2 months.
Bowman’s Gland: Secretes mucus.
Stimulation of Olfactory Cells:
Odorant dissolves in mucus and attaches to receptors on olfactory cilia.
Involves a cascade effect through G-proteins and second messengers, leading to depolarization and action potential generation in the olfactory nerve.
Quality of a Good Odorant:
Small (3-20 Carbon atoms), volatile, water-soluble, and lipid-soluble.
Facilitated by odorant-binding proteins in mucus.
Membrane Potential and Action Potential:
Resting membrane potential: -55mV.
Action potential frequency in the olfactory nerve increases with odorant strength.
Adaptation Towards the Sense of Smell:
Rapid adaptation within the first second, with further slow adaptation.
Psychological adaptation greater than receptor adaptation, involving feedback inhibition from the central nervous system.
Primary Sensations of Smell:
Camphoraceous, Musky, Floral, Pepperminty, Ethereal, Pungent, Putrid.
Odor Detection Threshold:
Examples: Hydrogen sulfide (0.0005 ppm), Methyl-mercaptan (0.002 ppm).
Some toxic substances are odorless at lethal concentrations.
Characteristics of Smell:
Odor blindness for single substances due to lack of appropriate receptor protein.
Behavioral and emotional influences of smell.
Transmission of Olfactory Signals:
From olfactory cells to glomeruli in the olfactory bulb, involving lateral inhibition.
Primitive, less old, and new olfactory systems with different path
TEST BANK for Operations Management, 14th Edition by William J. Stevenson, Ve...kevinkariuki227
TEST BANK for Operations Management, 14th Edition by William J. Stevenson, Verified Chapters 1 - 19, Complete Newest Version.pdf
TEST BANK for Operations Management, 14th Edition by William J. Stevenson, Verified Chapters 1 - 19, Complete Newest Version.pdf
263778731218 Abortion Clinic /Pills In Harare ,sisternakatoto
263778731218 Abortion Clinic /Pills In Harare ,ABORTION WOMEN’S CLINIC +27730423979 IN women clinic we believe that every woman should be able to make choices in her pregnancy. Our job is to provide compassionate care, safety,affordable and confidential services. That’s why we have won the trust from all generations of women all over the world. we use non surgical method(Abortion pills) to terminate…Dr.LISA +27730423979women Clinic is committed to providing the highest quality of obstetrical and gynecological care to women of all ages. Our dedicated staff aim to treat each patient and her health concerns with compassion and respect.Our dedicated group ABORTION WOMEN’S CLINIC +27730423979 IN women clinic we believe that every woman should be able to make choices in her pregnancy. Our job is to provide compassionate care, safety,affordable and confidential services. That’s why we have won the trust from all generations of women all over the world. we use non surgical method(Abortion pills) to terminate…Dr.LISA +27730423979women Clinic is committed to providing the highest quality of obstetrical and gynecological care to women of all ages. Our dedicated staff aim to treat each patient and her health concerns with compassion and respect.Our dedicated group of receptionists, nurses, and physicians have worked together as a teamof receptionists, nurses, and physicians have worked together as a team wwww.lisywomensclinic.co.za/
Report Back from SGO 2024: What’s the Latest in Cervical Cancer?bkling
Are you curious about what’s new in cervical cancer research or unsure what the findings mean? Join Dr. Emily Ko, a gynecologic oncologist at Penn Medicine, to learn about the latest updates from the Society of Gynecologic Oncology (SGO) 2024 Annual Meeting on Women’s Cancer. Dr. Ko will discuss what the research presented at the conference means for you and answer your questions about the new developments.
Ethanol (CH3CH2OH), or beverage alcohol, is a two-carbon alcohol
that is rapidly distributed in the body and brain. Ethanol alters many
neurochemical systems and has rewarding and addictive properties. It
is the oldest recreational drug and likely contributes to more morbidity,
mortality, and public health costs than all illicit drugs combined. The
5th edition of the Diagnostic and Statistical Manual of Mental Disorders
(DSM-5) integrates alcohol abuse and alcohol dependence into a single
disorder called alcohol use disorder (AUD), with mild, moderate,
and severe subclassifications (American Psychiatric Association, 2013).
In the DSM-5, all types of substance abuse and dependence have been
combined into a single substance use disorder (SUD) on a continuum
from mild to severe. A diagnosis of AUD requires that at least two of
the 11 DSM-5 behaviors be present within a 12-month period (mild
AUD: 2–3 criteria; moderate AUD: 4–5 criteria; severe AUD: 6–11 criteria).
The four main behavioral effects of AUD are impaired control over
drinking, negative social consequences, risky use, and altered physiological
effects (tolerance, withdrawal). This chapter presents an overview
of the prevalence and harmful consequences of AUD in the U.S.,
the systemic nature of the disease, neurocircuitry and stages of AUD,
comorbidities, fetal alcohol spectrum disorders, genetic risk factors, and
pharmacotherapies for AUD.
How STIs Influence the Development of Pelvic Inflammatory Disease.pptx
M. leprae.pptx
1. By Dr. Rakesh Prasad Sah
Associate Professor, Microbiology
2. • Leprosy: chronic mycrobacterial disease of ancient world
still affecting many parts of world maily Asia and Africa
• Leprosy Kushta Roga in Sushruta Samhita, 600 BC.
• Caused by Mycobacterium leprae first observed by
Hansen in 1868.
• First bacterial pathogen of human to described it still
remains least understood not possible to grow in artificial
culture medium.
3. • slender, slightly curved or straight bacillus.
• acid-fast, but less than tubercle bacillus 5% H2SO4 is
used.
• Intracellular & extracellular, single or in group in cells
present as bundles bound together by lipid like substance
‘glia’ & these masses are known as “globi”.
• Parallel rows of bacilli in globi give appearance of ‘Cigar
bndle’
ZN-
Staining
Uniform &
Solid
Fragmente
d &
granular
Dead
Bacilli
Live
Bacilli
Help to
monitor
efficacy
of the
treatme
nt
4. • slender, slightly curved or straight bacillus.
• acid-fast, but less than tubercle bacillus 5% H2SO4 is
used.
• Intracellular & extracellular,single or in group.in cells
present as bundles bound together by lipid like substance
‘glia’ & these masses are known as “globi”.
• Parallel rows of bacilli in globi give appearance of ‘Cigar
bndle’
ZN-
Staining
Uniform &
Solid
Fragmente
d &
granular
Dead
Bacilli
Live
Bacilli
Help to
monitor
efficacy
of the
treatme
nt
5. • Have not yet been grown on artificial culture media or
tissue culture.
• The most important animals used for experimental
infection with M. leprae include mouse and nine-banded
armadillos.
• Nine banded armadillo.
• Foot pad of mouse at low temp(200cafter inoculation
granuloma devlop at the site in 1—6 mths. if CMI depressed
(administration of antilymphocytic serum or thymectomy),
generalised infection resembling L.L.
• Armadillo L.L.
6. • One of best report from ICRC (Indian Cancer Research Institute)
acid fast bacilli isolated from leprosy patients employing human
foetal spinal ganglion cell culture.
• Other Animals
• Chimpanzees
• Monkeys
• Indian Pangolin
• Golden hamster
• Chipmunks
7. • Cell wall 4 layers. Inner peptidpglycan shape & rigidity
external to this is lipoarabinomannan—B (LAM-B),
attach to this is mycolic acid layer. outermost layer’s major
component is phenolic glycolipid-1(PGL-1). LAM-B, PGL-1—
used for serodignosisPGL-1supress CMI.
• 18kda, 28kda,----
8. • Chronic granulomatous disease.
• Bacilli localize primarily in the
• Skin
• Peripheral nerves
• Nasal Mucosa
• Tissue or organ may be involved
• Source
• infected patients.
• Mode of transmission
• droplets
• skin lesions.
• I.P.—Long, Variable.
Preference Of
Bacilli
Lower
Temperature
Superficial &
Cooler
Tissues
9. • Types
• Lepromatous,
• Tuberculoid
• Diamorphous
• Indeterminate
• CMI status-determines type.
• Indeterminate occupies position between L.L. & T.T.
• Type of leprosy changes with chemotheraopy &
alternation in immune status.
10. • Seen in individuals either CMI is low or absent.
• Severe form & poor prognosis.
• More infectious, Lepromin negative deficient CMI.
• High conc. of humoral abs No protective role.
• Develops nodular lesion on
• Face
• Ear lobes
• Hand
• Feet
• Less commonly on trunk
11. • Is slow and symmetric thickening of peripheral nerves &
anesthesia
• As a result of loss of sensation nodules ulcerate &
secondary infection occur distortation & mutilation of
extremities.
• Multibacillary.
• Skin lesions contain many macrophages packed
with bacilli.
• Lepra bacilli present in large no. in
• Skin lesions
• Mucosa of nose
• Mouth
• Upper respiratory tract
• Bacilli shed in
• Sweat
• Nasal
• Oral secretions.
12. • Localised form of disease CMI good.
• few skin lesions Non elevated hypo or hyper
pigmented macular patches involving
• Face
• Limbs
• Trunk
• Bacilli very few of absent tissues.
• Lepromin positive intact CMI
• abs. rarely produced.
extend into
big nerve
trunks
Involvement
of local
nerves
become
thickened,
hard and
tender
lead to
deformities
of limbs
13. Feature Lepromatous
leprosy
Tuberculoid
Leprosy
Lepra Bacilli in
lesion
Numerous Scanty
CMI Deficient / absent Adequate
Lepromin test Negative Positive
Infectivity Highly infectious Usually non
infective
Mycobacterial
Antibodies
Abundant Rarely produced
14. • Lesion resembels TL but bacteriological &
immunological picture is of LL.
• Shifting of the form to any of the two extreme type
depends upon chemotherapy & alteration in immune
status of host.
15. • Neither characteristic of TT nor LL.
• Lesions in the form of maculoanashthetic patches.
• Type 1 Reaction (Reversal Reaction)
• seen in borderline patient who developed CMI and shift toward
tuberculoid spectrum.
• It may lead to permanent nerve damage.
• This is due to DTH.
• Type 2 ( erythema nodosum leprosum)
• seen in LL patients under treatment.
• This is due to formation of Ag-Ab complexes.
• Clinically crops of red nodules appears on skin which last for 1-2 days.
16. Characters TT BT BB BL LL
Bacilli in the skin - +/- + ++ +++
Nasal secretion - - - + +++
Granulomma formation +++ ++ + - -
Lepromin Test +++ - +/- - -
Ab to M. Leprae +/- +/- + ++ +++
17. • First described by Mitsuda in 1919.
• Original lepromin antigen- boiled, emulsified, lepromatous
tissue rich in lepra bacilli.
• Nowadays, Lepromin-A or Lepromin-H.
• Are of 2 types-
• 1) Integral Antigen
• 2) Bacillary Lepromin.
18. • Procedure 0.1ml intradermal.
• Biphasic response
• Early reaction of Fernandez erythema & induration in 24-48 hrs,
remains for 3-5 days. Histologicaly serous exudates with
lymphocytic infiltration.
• Late reaction of Mitsuda appears after 1-3 wks of injection and
peak in 4wks reaction the form of nodule-may ulcerate.
Histologically infiltration with lymphocytes, epitheloid, giant
cells.
• Mitsuda indicates resistance to leprosy.
19. • Classification of leprosy.
• Assessment of prognosis.
• Assessment of resistance.(field workers)
20. • Specimen : Nasal Mucosa, skin lesions, & ear lobules.
• The specimens from skin are obtained by “slit and scrape”
method.
• Skin- six diff areas including
• buttocks
• chin
• cheek,
• forehead
• ears.
Smear on slide
Air dry & fix
Stain (ZN-
Pinch the Sight
Scrape & Collect
material
Incise
21. • AFB staining
• Grading-
• 1-10 in 100 fields ---- 1+
• 1-10 in 10 fields----- 2+
• 1-10 per field--------3+
• 10-100 per field-----4+
• 100-1000 per field---5+
• More than 1000 bacilli, clumps & globi in every field = 6+
22. • Bacteriological Index:-
• Defined as number of total bacilli in tissue.
• Calculated by grades (number of pluses, +s) scored in all smears divided
by no. of smears taken.
• E.g. if 7 smears examined have total of 14+ BI will be 2.
• For calculation minimum of
• 4 skin lesions
• nasal swab and
• both ear lobes examined.
• Morphological Index
• Defined as percentage of uniformly stained bacilli out of total no. of bacilli
counted.
• Useful for assessing chemotherapy
23. • Animal inoculation
• Lepromin test: - not diagnostic but to assess prognosis
and response to treatment.
• Serological test: Anti PGL-1 Antibodies detection. High
titer in LL and LOW in TL.
24. • Chemotherapy : Dapsone.
Emergence of Dapsone resistance hence WHO
recommended MDT.
• Immunotherapy:
Efforts to induced effective CMI by IV inj of transfer factor,
and extract of lymphocytes from patients suffering from
TL etc.
• Administration Of Vaccine: