DNA Replication.pdf
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This document discusses DNA replication and the central dogma. It covers the basic requirements for DNA replication including substrates, templates, enzymes, and primers. The stages of replication - initiation, elongation, and termination - are described. Key aspects of the replication process are explained, such as semi-conservative mechanism, unwinding of DNA, formation of replication forks, and bidirectional replication. Differences between prokaryotic and eukaryotic DNA replication are highlighted. Finally, various inhibitors of DNA replication are listed.
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DNA Replication.pdf
- 1. 26-03-23 19:15:11 S.Karthikumar Assistant Professor Department of Biotechnology Kamaraj College of Engineering and Technology Virudhunagar
- 11. 26-03-23 19:15:11 ENZYMES DNA Gyrase DNA Helicase Primase DNA Polymerase - I SSB DNA Topoisomerase - III DNA Ligase Ter binding protein Enzymes and Proteins Enzymes and Proteins
- 13. STAGES STAGES Initiation Initiation Elongation Elongation Termination Termination 26-03-23 19:15:11
- 14. Unwinding and formation of replication fork Unwinding and formation of replication fork 26-03-23 19:15:11 Replication bubble Replication bubble
- 17. 26-03-23 19:15:11 5’ 3’ 5’ Primase synthesizes PRIMER Primase synthesizes PRIMER Single stranded binding protein Single stranded binding protein
- 18. 26-03-23 19:15:11 5’ 5’ 3’ 3’ 5’ 3’ Parental DNA Leading strand Laging strand 5’ 3’ 5’ Okazaki fragments Okazaki fragments Primer Primer Gap filled byDNA Ligase Gap filled byDNA Ligase Elongation by DNA Polymerase III Elongation by DNA Polymerase III Primer is removed by DNA Polymerase I Primer is removed by DNA Polymerase I
- 23. Termination sequences “Ter” direct termination Termination sequences “Ter” direct termination 26-03-23 19:15:11
- 25. DNA →DNA Bidirectional Primers • Semiconservative mechanism • Begins at origin • Synthesis5→3’ • Read 3’→5’ • Leading strand • Laging strand 26-03-23 19:15:11
- 26. Differences between prokaryotic and eukaryotic DNA replication 26-03-23 19:15:11 Features Prokaryotes Eukaryotes RNA primer length 50 nucleotides 9 nucleotides DNA polymerase I,II,III α,β,γ,δ,ε Number of origins Single Multiple Nucleotide length of Okazaki 1000-2000 nucleotides 200 nucleotides Rate of replication 500 nucleotides/sec 50 nucleotides/sec
- 29. Recall - 1 26-03-23 19:15:11