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DNA is the genetic material
Transforming
Principle
Phage T2
Transfection
•DNA is the genetic material in
eukaryotes
•It can be transferred between different
species and yet remain functional
DNA is a double helix
Nucleic acid
Nucleosides
Sequences of bases is the form in which
genetic information is carried
Watson & Crick DNA double helix
•X-ray diffraction data
•Constant diameter of DNA
•G= C, A=T
Mutations change the sequence of
DNA
Mutations
•Spontaneous - Due to normal cellular operations
•Induced mutations - Due to mutagens
Nitrous acid
effect
BrdU
effect
Triplet code
Triplet code
•Genetic code
•Genetic information
•Codon
Frame-shift
mutations
Genetic code
•No overlapping
•Use fixed starting point
Blocked reading frames
mRNA
Gene may be longer than Prt
sequence
Gene may be longer than Prt
sequence
Eukaryotic genes are often interrupted
Organization of interrupted genes may
be conserved
Globin genes
Introns in Vertebrates
Exons in Eukaryotes
C-value
C-value
C-value
Amino acids
Proteins are dehydration polymers of amino acids
All 20 common amino acids are α-amino acids
They differ from each other in their side chains, or R groups
Stereoisomerism
Classification
Classification
Classification
Classification
Classification
Twenty publications that
influenced present view

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