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UV-VISIBLE
SPECTRO
PHOTOMETER
INDEX
 Introduction
 Principle
 Instrumentation
 Types
 Application
INTRODUCTION
 A spectrophotometer is an instrument that measures the amount of
photons absorbed by a sample after it is passed through its solution.
 UV-Visible spectrophotometer uses UV and visible range of
electromagnetic radiation spectrum.
 Electromagnetic spectrum:
PRINCIPLE
 BEER – LAMBERT LAW
It states that the absorbance of light by a material in a solution is directly
proportional to its concentration in that solution.
A = ϵlc
Where,
 A – absorbance
 ϵ - molar absorptivity
 l – length of solution
 c – concentration
 Transmittance (T) = It / Io
 A = log1/ T = log Io / It
INSTRUMENTATION
TYPES
Single beam
Double beam
APPLICATIONS
 1. Concentration measurement
 2. Detection of impurities
 3. Chemical kinetics
 4. Detection of functional group
Thank you

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