This document discusses the safe use and storage of chemicals and reagents in laboratories. It outlines specific guidelines for different types of chemicals including flammable, corrosive, toxic, oxidizing, explosive, and carcinogenic chemicals. Recommendations are provided for storage, labeling, and handling of small quantities of each chemical type. The document also discusses planning laboratory safety through adequate space, ventilation, protective equipment, and disposal of contaminated materials.
Handling of chemicals, handling of acids, ethers, calibration of burette, pipette, Heating methods, stirring methods, filtratio techniques, chemical balance and single pan balance
Laboratory Hazards, Accidents and Safety RulesTapeshwar Yadav
Injury, damage and loss by fire can be minimized when laboratory staff:
Understand how fires are caused and spread;
Reduce the risk of fire by following fire safety regulations at all times;
Know what to do if there is a fire in their laboratory;
Know how to use fire fighting equipment;
Know how to apply emergency First Aid, for burns.
Handling of chemicals, handling of acids, ethers, calibration of burette, pipette, Heating methods, stirring methods, filtratio techniques, chemical balance and single pan balance
Laboratory Hazards, Accidents and Safety RulesTapeshwar Yadav
Injury, damage and loss by fire can be minimized when laboratory staff:
Understand how fires are caused and spread;
Reduce the risk of fire by following fire safety regulations at all times;
Know what to do if there is a fire in their laboratory;
Know how to use fire fighting equipment;
Know how to apply emergency First Aid, for burns.
Laboratory safety rules are a major aspect of every clinical lab.
Each student in clinical laboratory must follow specific safety rules and procedures.
This slide gives you details about the following:
Safety precautions.
Rules and regulations to be followed inside laboratory.
Different type of laboratory hazards.
How to deals with laboratory accident incidents.
Diagrammatic representation of dress codes & rules.
bio safety cabinets.
Dress codes for technicians dealing with radioactive materials
sterilization of whole room (Fumigation)
Laboratory safety rules are a major aspect of every clinical lab.
Each student in clinical laboratory must follow specific safety rules and procedures.
This slide gives you details about the following:
Safety precautions.
Rules and regulations to be followed inside laboratory.
Different type of laboratory hazards.
How to deals with laboratory accident incidents.
Diagrammatic representation of dress codes & rules.
bio safety cabinets.
Dress codes for technicians dealing with radioactive materials
sterilization of whole room (Fumigation)
Who We Are | What We Do
New Era Education Institute is milestone the way for ambitious students who wish to start out a successful career in MBBS Abroad. We are most excellent at helping you to fulfill your dream by providing continuous support for all of your MBBS Abroad admission needs. Our advisory and value-added solutions include MBBS Abroad admission, payment processing, student visa, accommodation
Working in a laboratory usually involves working with various chemical, physical, and biological hazards. Because the hazards vary from laboratory to laboratory, employers must address the hazards specific to their laboratories. Standard precautions are meant to reduce the risk of transmission of blood borne and other pathogens from both recognized and unrecognized sources. They are the basic level of infection control precautions which are to be used, as a minimum, in the health care settings.
Essential Textbook of Biochemistry For Nursing (B.Sc.Nursing & PBN)Tapeshwar Yadav
I have relished teaching Biochemistry during my more than Ten years teaching experience in a medical, dental, nursing and health science colleges. It was because of constant inspiration from my students that I could come up with Essentials Textbook of Biochemistry for Nursing book, which hopefully would meet the inadequacies the students face in other books. In this age when the concepts in this subject are constantly changing, this book attempts to summarise the fundamentals and current state of knowledge in Biochemistry.
Biochemistry has been primarily written for the students of B.Sc. Nursing & Post Basic of Nursing (PBN) in such a way that it will also be suitable for General Medicine, Radiography, Physiotherapy, Ayurveda, Optometry, Dental and Nursing. This book can also be used as Reference for B.Sc. MLT, Bachelor of Pharmacy (B. PHARMA), Bachelor of Public Health (BPH), Bachelor of Physiotherapy (BPT), B. Ophthalmology, Bachelor of Radiography (BRT) and Biomedical Engineering students of Tribhuvan University, Purbanchal University, Kathmandu University and Pokhara University. Similarly, it will be equally useful for all the teachers, academic writers and those who are involved directly or indirectly in teaching and practising Health Sciences.
This is a basic book on Essential Textbook of Biochemistry for Nursing. The book thoroughly discusses some of the major concepts of Biochemistry and provides adequate information to help the students understand its implications in various areas of the subject. Furthermore, the book aims at equipping the students with practical cum theoretical skills. The book covers almost all the topics which have been prescribed in the Syllabus.
This is an introductory course to Biochemistry and is about medical biochemistry including the biochemical processes of - digestion & absorption of foods, metabolism of different kinds of foods & their disturbance effects in our body together with the physiological roles of different kinds of vitamins & enzymes.
The book consists of Theory as well as Practical portion. The author has tried his best to make all the concepts of each unit as lucid and simple as required for the students with supportive examples, samples, diagrams, clinical disorders and practical works. The ultimate purpose of this book is to equip the reader with comprehensive knowledge in Biochemistry with reference to basic as well as clinical aspects.
At last, I have made every effort to make the book error free, I am under no illusion. I expect constructive comments and suggestions from learners and teachers who use this book which will obviously help me in improving the future edition of the book.
Amino acids are a group of organic compounds containing two functional groups amino and carboxyl. The amino group (-NH2) is basic while the carboxyl group (-COOH) is acidic in nature.
The plasma in the liquid medium of blood (55%) in which the cell components namely Erythrocytes, Leucocytes and Platelets are suspended.
If anticoagulated blood is centrifuged, the plasma separates out as a supernatant while the cells remain at the bottom.
Plasma consists of water electrolytes metabolites nutrients proteins and hormones.
Most of the plasma proteins are synthesized in the liver.
Plasma proteins are separated by electrophoresis.
The word protein is derived from the Greek word ‘Proteios’ which means holding the first place. Berzelius (Swedish chemist) suggested the name proteins to the group of organic compounds that are important to life.
Proteins are the most abundant organic molecules of the living system.
They occur in every part of the cell and constitute about 50% of the cellular dry weight.
Proteins form the fundamental basis of structure and function of life.
Out of the total dry body weight, 3/4th are made up of proteins.
Proteins are used for body building; all the major structural and functional aspects of the body are carried out by protein molecules.
Proteins are high molecular weight polypeptides containing α-amino acids joined together by peptide linkage (-CO-NH).
The endocrine system consists of a network of ductless glands that secrete chemicals (called hormones) that affect the function of specific organs within the body, thus regulating many of the intricate functions of the body itself.
These ductless glands secrete their hormones directly into the bloodstream, as opposed to releasing them externally through ducts (as do the sweat glands and the oil glands).
The field of medicine that deals with the study of the endocrine system and the treatment of the diseases and disorders of the endocrine system is known as endocrinology.
The physician who specializes in the medical practice of endocrinology
is known as an endocrinologist.
Carbohydrates are the most abundant organic molecules in nature.
They are commonly known as saccharides or sugars.
They are primarily composed of the elements carbon, hydrogen and oxygen.
The name carbohydrate literally means “hydrates of carbon”.
Carbohydrates are widely distributed in nature in plants and animals.
The most important carbohydrate found in plants is starch.
It occurs abundantly in roots, tubers, vegetables and grains. The carbohydrate found in animals is glycogen.
It is a storage form of carbohydrate present in liver and muscles, which serves as important sources of energy for vital activities.
This field combines biology as well as chemistry to study the chemical structure of a living organism
Biochemistry is a basic science which deals with chemical nature and chemical behaviour of living matter and with the reactions and processes they undergo.
“The branch of science dealing with the study of all the life processes such as control and coordination within a living organism is called Biochemistry”
Medical parasitology : study of parasites that infect human, diseases caused by them, clinical picture, their diagnosis, treatment and prevention as well as controls.
It involves drug development, epidemiological studies and study of zoonoses.
To know various terms related to parasitology.
To know about general parasites and parasitic infections.
To get knowledge about laboratory diagnosis and its importance.
To gain idea about general epidemiological aspects of parasites that affect human.
Apply basic methods of specimen collection , preservation and processing in lab.
To prevent ourselves from these infections and apply control measures.
Microbiology is the study of
living organisms of microscopic
size which includes bacteria ,
Fungi , Algae , Protozoa and Viruses. It is concerned with the forms, structure , reproduction , physiology , metabolism and classification.
Principle Of Microbiology
Medical microbiology deals with the causative agent of the infectious disease of the human , the ways in which they produce disease in the body and essential information for diagnosis and treatment.
Hematology is the branch of medicine, that is concerned with the study of blood, blood forming organs and blood diseases. It includes study of etiology, diagnosis, treatment, prognosis and prevention of blood diseases .
After the completion of this presentation we will know about:
What is hematology and its purpose.
hematology laboratory.
Blood and its compositions and collections
Hematology lab equipment's
Some hematological tests , disease and hazards too.
Biochemistry is the study of the structure and function of biological molecules such as proteins, nucleic acids, carbohydrates and lipids.
Biochemistry is the study of the chemistry of living things. This includes organic molecules and their chemical reactions.
Biochemistry deals with body substance like enzymes, carbohydrates, amino acids, fats, proteins, hormones, DNA, RNA, pigments etc.
The major objective of biochemistry is the complete understanding of all chemical processes associated with living cells at the molecular level. Some of the objectives can be listed as follows:
1. Isolation, structural elucidation and the determination of mode of action of biomolecules.
2. Identification of disease mechanisms.
3. Study of in born errors of metabolism.
4. Study of oncogenes in cancer cells.
5. The relationship of biochemistry with the genetics, physiology, immunology, pharmacology, toxicology etc.
Biochemistry is related to almost all the life sciences and without biochemistry background and knowledge, a through understanding of health and well-being is not possible.
It is a well known fact that metal ions have a profound effect on cellular processes
The importance or the role that ions play in cellular activity can be gauged by the fact that most cells maintain a very critical Na+ & k+ balance between the extracellular and the intracellular spaces.
Any distribution in this critical balance is to the cellular metabolism through a drastic change in the osmotic pressure resulting in cellular swelling.
An ISE operates an exactly the same principles as a PH electrode
In fact, a PH electrode is a type of ion selective electrode sensitive to hydrogen ion.
Just like a PH electrode, the electrode body contains a reference solution and an metal reference electrode
Safety cabinets are intended to protect a laboratory worker from aerosols and airborne particles.
They will not protect the person from spillages and the consequences of mishandling and poor technique.
Aerosol particles of less than 5 µm in diameter and small droplets of 5–100 µm in diameter are not visible to the naked eye.
The laboratory worker is generally not aware that such particles are being generated and may be inhaled or may cross contaminate work surface materials.
BSCs, when properly used, have been shown to be highly effective in reducing laboratory-acquired infections and cross-contaminations of cultures due to aerosol exposures. BSCs also protect the environment.
Most BSCs use high efficiency particulate air (HEPA) filters in the exhaust and supply systems.
The exception is a Class I BSC, which does not have HEPA filtered supply air.
The application of knowledge, techniques and equipment to prevent a personal laboratory and environmental exposure to potentially infectious agents or biohazard is known as biosafety.
Biosafety defines the containment conditions under which infectious agents can be safely manipulated.
The objective of containment is to confine biohazard and to reduce the potential exposure of the laboratory worker, persons outside of the laboratory, and the environment to potentially infectious agents.
A pipette (also called a point or a pipettor) is a laboratory instrument used to transfer a measured volume of liquid.
Pipettes are commonly used in chemistry and molecular biology research as well as clinical biochemistry tests.
Pipettes come in several designs for various purposes with different levels of accuracy and precision, from single piece flexible plastic transfer pipettes to more complex adjustable or electronic pipettes.
A pipette works by creating a vacuum above the liquid-holding chamber and selectively releasing this vacuum to draw and dispense liquid.
Revised Curriculum of Certificate in Medical Laboratory Technology(CMLT) by C...Tapeshwar Yadav
This curriculum of 3 years Certificate in Medical Laboratory Technology is designed to produce middle level skilled laboratory personnel equipped with knowledge, skills and attitudes of medical laboratory with a view to provide diagnostic, curative, preventive and promotive laboratory services to the community. Such technicians collect specimens, process, and perform tests to analyze body fluids, tissue, and other substances. The graduates perform lab procedures and maintain instruments. The graduates are expected to perform tests that help other healthcare professionals such as physicians to detect, diagnose, and treat diseases.
The program extends over three academic years. The first year course focuses on basic science and foundational subjects, the second year course focuses on theory and practical parts of basic medical laboratory subjects. Simultaneously the third year is given to the application of learned skills and knowledge in the comprehensive practical settings, in hospitals and medical laboratory. The graduates will have career opportunities in hospitals, diagnostic laboratories, clinics, industry and physicians' offices, research centers, blood bank, crime investigating laboratories etc. It is based on the code of conduct of Nepal Health professional Council.
Clinical Pathology is the application of laboratory techniques to find out the cause of disease. Clinical pathology laboratory involves all aspect of the medicine ranging from the field of biochemistry, microbiology, Parasitology, haematology, immunology and cytogenetics etc. Clinical pathology laboratory perform qualitative and quantitative analysis of body fluid such as urine, blood, CSF, sputum, other body fluid such as synovial fluid, peritoneal fluid, pericardial fluid and plural fluid. These determinations are useful in diagnosing various clinical conditions such as diabetes mellitus, jaundice, gout, hyperlipidemia, pancreatitis, rickets, etc. The clinical pathological tests are very useful in determining the severity of diseases of many organs such as liver, stomach, heart, kidneys, brain as well as the endocrine disorders and related status of acid-base balance of the body. The clinical pathology tests, in relation to the various clinical conditions can be applicable for:-
1) Reveal the causes of the diseases
2) Screen easy diagnosis
3) Suggest effective treatment
4) Assist in monitoring progress of a pathological condition and
5) Help in assessing response to therapy
Modern medicine says that: Your practice of medicine will be as good as your understanding of pathology.
Biochemistry is a basic science which deals with chemical nature and chemical behaviour of living matter and with the reactions and processes they undergo.
Biochemistry involves the study of:
Chemical constituents of living matter.
Chemical changes which occur in the organism during digestion, absorption and excretion.
Chemical changes which occur during growth and multiplication of the organism.
Transformation of one form of chemical constituent to the other.
Energy changes involved in such transformation.
Note:- The term “Biochemistry” was first introduced by German chemist Carl Neuberg in 1903 from Greek word “bios” means “life”.
It is mainly deals with the biochemical aspects that are involved in several conditions.
The results of qualitative and quantitative analysis of body fluids assist the clinicians in the diagnosis, treatment and prevention of the disease and drug monitoring, tissue and organ transplantation, forensic investigations and so on.
Various biological fluids subjected to chemical tests and assays include blood, plasma, serum, urine, cerebrospinal fluid (CSF), ascetic fluid, pleural fluid, faeces, calculi and tissues.
Note:- Modern day medical practice is highly dependent on the laboratory analysis of body fluids, especially the blood. The disease manifestations are reflected in the composition of blood and other tissues.
Hence, the demarcation of abnormal from normal constituents of the body is another aim of the study of clinical biochemistry.
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Title: Sense of Smell
Presenter: Dr. Faiza, Assistant Professor of Physiology
Qualifications:
MBBS (Best Graduate, AIMC Lahore)
FCPS Physiology
ICMT, CHPE, DHPE (STMU)
MPH (GC University, Faisalabad)
MBA (Virtual University of Pakistan)
Learning Objectives:
Describe the primary categories of smells and the concept of odor blindness.
Explain the structure and location of the olfactory membrane and mucosa, including the types and roles of cells involved in olfaction.
Describe the pathway and mechanisms of olfactory signal transmission from the olfactory receptors to the brain.
Illustrate the biochemical cascade triggered by odorant binding to olfactory receptors, including the role of G-proteins and second messengers in generating an action potential.
Identify different types of olfactory disorders such as anosmia, hyposmia, hyperosmia, and dysosmia, including their potential causes.
Key Topics:
Olfactory Genes:
3% of the human genome accounts for olfactory genes.
400 genes for odorant receptors.
Olfactory Membrane:
Located in the superior part of the nasal cavity.
Medially: Folds downward along the superior septum.
Laterally: Folds over the superior turbinate and upper surface of the middle turbinate.
Total surface area: 5-10 square centimeters.
Olfactory Mucosa:
Olfactory Cells: Bipolar nerve cells derived from the CNS (100 million), with 4-25 olfactory cilia per cell.
Sustentacular Cells: Produce mucus and maintain ionic and molecular environment.
Basal Cells: Replace worn-out olfactory cells with an average lifespan of 1-2 months.
Bowman’s Gland: Secretes mucus.
Stimulation of Olfactory Cells:
Odorant dissolves in mucus and attaches to receptors on olfactory cilia.
Involves a cascade effect through G-proteins and second messengers, leading to depolarization and action potential generation in the olfactory nerve.
Quality of a Good Odorant:
Small (3-20 Carbon atoms), volatile, water-soluble, and lipid-soluble.
Facilitated by odorant-binding proteins in mucus.
Membrane Potential and Action Potential:
Resting membrane potential: -55mV.
Action potential frequency in the olfactory nerve increases with odorant strength.
Adaptation Towards the Sense of Smell:
Rapid adaptation within the first second, with further slow adaptation.
Psychological adaptation greater than receptor adaptation, involving feedback inhibition from the central nervous system.
Primary Sensations of Smell:
Camphoraceous, Musky, Floral, Pepperminty, Ethereal, Pungent, Putrid.
Odor Detection Threshold:
Examples: Hydrogen sulfide (0.0005 ppm), Methyl-mercaptan (0.002 ppm).
Some toxic substances are odorless at lethal concentrations.
Characteristics of Smell:
Odor blindness for single substances due to lack of appropriate receptor protein.
Behavioral and emotional influences of smell.
Transmission of Olfactory Signals:
From olfactory cells to glomeruli in the olfactory bulb, involving lateral inhibition.
Primitive, less old, and new olfactory systems with different path
Couples presenting to the infertility clinic- Do they really have infertility...Sujoy Dasgupta
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TEST BANK for Operations Management, 14th Edition by William J. Stevenson, Verified Chapters 1 - 19, Complete Newest Version.pdf
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Report Back from SGO 2024: What’s the Latest in Cervical Cancer?bkling
Are you curious about what’s new in cervical cancer research or unsure what the findings mean? Join Dr. Emily Ko, a gynecologic oncologist at Penn Medicine, to learn about the latest updates from the Society of Gynecologic Oncology (SGO) 2024 Annual Meeting on Women’s Cancer. Dr. Ko will discuss what the research presented at the conference means for you and answer your questions about the new developments.
New Drug Discovery and Development .....NEHA GUPTA
The "New Drug Discovery and Development" process involves the identification, design, testing, and manufacturing of novel pharmaceutical compounds with the aim of introducing new and improved treatments for various medical conditions. This comprehensive endeavor encompasses various stages, including target identification, preclinical studies, clinical trials, regulatory approval, and post-market surveillance. It involves multidisciplinary collaboration among scientists, researchers, clinicians, regulatory experts, and pharmaceutical companies to bring innovative therapies to market and address unmet medical needs.
Ethanol (CH3CH2OH), or beverage alcohol, is a two-carbon alcohol
that is rapidly distributed in the body and brain. Ethanol alters many
neurochemical systems and has rewarding and addictive properties. It
is the oldest recreational drug and likely contributes to more morbidity,
mortality, and public health costs than all illicit drugs combined. The
5th edition of the Diagnostic and Statistical Manual of Mental Disorders
(DSM-5) integrates alcohol abuse and alcohol dependence into a single
disorder called alcohol use disorder (AUD), with mild, moderate,
and severe subclassifications (American Psychiatric Association, 2013).
In the DSM-5, all types of substance abuse and dependence have been
combined into a single substance use disorder (SUD) on a continuum
from mild to severe. A diagnosis of AUD requires that at least two of
the 11 DSM-5 behaviors be present within a 12-month period (mild
AUD: 2–3 criteria; moderate AUD: 4–5 criteria; severe AUD: 6–11 criteria).
The four main behavioral effects of AUD are impaired control over
drinking, negative social consequences, risky use, and altered physiological
effects (tolerance, withdrawal). This chapter presents an overview
of the prevalence and harmful consequences of AUD in the U.S.,
the systemic nature of the disease, neurocircuitry and stages of AUD,
comorbidities, fetal alcohol spectrum disorders, genetic risk factors, and
pharmacotherapies for AUD.
Flu Vaccine Alert in Bangalore Karnatakaaddon Scans
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This year, the flu season is expected to coincide with a potential increase in other respiratory illnesses. The Karnataka Health Department has launched an awareness campaign highlighting the significance of flu vaccinations. They have set up multiple vaccination centers across Bangalore, making it convenient for residents to receive their shots.
To encourage widespread vaccination, the government is also collaborating with local schools, workplaces, and community centers to facilitate vaccination drives. Special attention is being given to ensuring that the vaccine is accessible to all, including marginalized communities who may have limited access to healthcare.
Residents are reminded that the flu vaccine is safe and effective. Common side effects are mild and may include soreness at the injection site, mild fever, or muscle aches. These side effects are generally short-lived and far less severe than the flu itself.
Healthcare providers are also stressing the importance of continuing COVID-19 precautions. Wearing masks, practicing good hand hygiene, and maintaining social distancing are still crucial, especially in crowded places.
Protect yourself and your loved ones by getting vaccinated. Together, we can help keep Bangalore healthy and safe this flu season. For more information on vaccination centers and schedules, residents can visit the Karnataka Health Department’s official website or follow their social media pages.
Stay informed, stay safe, and get your flu shot today!
2. Introduction:
Even in the smallest laboratory, dangerous
chemicals are used directly or incorporated into
stains and reagents.
Hence the correct handling and storage of
hazardous chemicals is essential to prevent injury
and damage.
In addition to this, to reduce accidents caused by
chemicals, labeling is very important.
3. 1. Flammable chemicals:
These include ether, xylene, toluene, methanol,
ethanol, other alcohol, glacial acetic acid, acetone,
and acetic anhydride.
Alcoholic Romanovsky stains and acid alcohol
solutions are also highly flammable.
Storage:
Flammable chemicals should be stored in a fire proof
metal box at ground level, preferably in and out side
cool and locked store.
If a metal box is not available, at least a container well
lined with tin foil should be used.
N.B: Only small quantities of flammable solvents should
be kept on lab, benches and shelves.
4. Contd…
Safe Use:
Before opening a bottle containing a flammable
solvent, check that there is no open flame such
as that from a Bunsen burner. Do not light match
near flammable chemicals.
N.B: Never heat a flammable liquid over a Bunsen
burner or lighted gas.
5. 2.Corrosive chemicals:
Corrosive chemicals include strong acids such as
concentrated sulfuric acid, nitric acid, glacial
acetic acid, trichloroacetic acid, ortho -
phosphoric acid, and caustic alkalis such as
sodium hydroxide (caustic soda) and potassium
hydroxide (caustic potash).
Storage:
Corrosive chemicals should be stored at low level
to avoid any serious injury, which could be
caused if they are accidentally knocked off a
shelf.
6. Contd…
Safe use:
Never mouth pipette corrosive chemicals instead use
automatic pipettes.
The accidental swallowing of corrosive chemicals can
cause severe injury because such chemicals destroy
living tissues.
Always pour corrosive chemicals at below eye level,
slowly and with great care to avoid splashing.
When opening a container of corrosive chemicals,
and when pouring it, wear protective materials.
N.B: When diluting concentrated acids in general and
sulfuric acid in particular, ‘ALWAYS’ add the acids to
the water. This is because adding of a small amount
of water to concentrated acids produces sufficient
amount of heat that can break a glass container,
which can cause damage and even chemical burn.
7. 3.Toxic, harmful, and irritating
chemicals:
Toxic chemicals are those chemicals which can
cause death or serious ill-health if swallowed or
inhaled, or if the chemical is allowed to come into
contact with the skin.
Examples of toxic chemicals
include potassium cyanide, sodium nitroprusside,
formaldehyde solution, chloroform, barium
chloride and methanol.
Harmful chemicals can cause ill- heath if
swallowed and inhaled, or by skin contact.
Example, iodine and sulphanilic
acid chemicals can cause inflammation and
irritation of the skin, mucous membranes, and
8. Contd…
Storage:
Highly toxic chemicals such as potassium cyanide
must be kept in a locked cupboard. Stock solutions or
solids of harmful and irritating chemicals should be
stored safely in cap board, not on an open shelf.
Safe use:
Handle toxic, harmful and irritating chemicals with
great care by wearing protective gloves. Always lock
away highly toxic chemicals immediately after use.
Keep the lab, well ventilated while the chemicals are
being used.
N.B: Never mouth pipette any chemicals, instead use
automatic pipette or dispenser or pipette filler.
9. 4.Oxidizing chemicals:
These chemicals include chlorates, perchlorates,
strong peroxides, potassium dichromate, and
chromic acid.
Storage:
Oxidizing chemicals must be stored away from
organic materials and reducing agents. They can
produce much heat when in contact with other
chemical, especially flammable chemicals.
Safe use:
Handle oxidizing chemicals with great care. Most
are dangerous to skin and eyes and when in
contact with reducing agents.
10. 5.Explosive chemicals:
Heat, flame, or friction can cause explosive
chemicals to explode. An example of
explosive chemical is picric acid, which must
be stored under water.
If picric acid is allowed to dry, it can become
explosive.
This can occur if the chemical is left to dry in
pipes without being flushed away with
adequate amount of water.
11. 6.Carcinogens:
A chemical that can cause cancer by
ingestion, inhalation, or by skin contact is
known as a carcinogen.
Chemicals with proven carcinogenic
properties include benzene, Ortho - tolidine,
alpha and beta- naphthylamine, nitrosamines
and selenite.
The risk in handling of these chemicals is
proportional to the length and frequency of the
exposure and the concentration of the
chemical.
12. Contd…
Storage:
Carcinogens should be kept in closed containers
and labeled as ‘carcinogenic, handle with special
precautions’.
Safe use:
Always wear protective plastic or rubber gloves,
and face mask when handling carcinogenic
chemicals.
Carcinogens must not be allowed to come in
contact with the skin because some carcinogens
can be absorbed through the skin such as beta -
naphtylamine.
13.
14. Planning for safety:
A laboratory should be planned not only for efficient
work but also designed with a view to eliminate
accidents.
The following are among the features of a safety
designed or planned and organized laboratory.
Adequate floor, bench and storage space for staff to
work safely;
Ample light is essential, especially in the examination
areas of the laboratory;
A sufficient supply of wall electric points to avoid the
use of adapters;
Overcrowding must be avoided;
Good ventilation is essential with adequate provision
of fume cupboards;
15. Contd…
There should be a system for marking ‘’high
risk’’ specimens.
Discard containers that contain infectious
microorganisms after each use;
The floor should be well constructed with a
surface that is non-slippery, impermeable to
liquids and resistant to those chemicals used in
the laboratory;
Walls should be smooth, free from cracks,
impermeable to liquids and easily washable;
Doors of the of the lab, should be opened to the
out side direction;
16. Contd…
Sectioning of the lab, into separate rooms or working
areas with definite places (for patients, visitors, and
reception of specimens);
Bench surfaces should be without cracks, washable
and resistant to the disinfectants and chemicals used
in the laboratory;
An adequate number of hand basins with running
water is essential.
Provision of protective clothing; Example, gown
Fire extinguishers should be placed at accessible
points. If extinguishers are not available several
buckets of sand must be provided;
17. Contd…
Ensure that all work in the laboratory is done with
a safety conscious attitude;
All staff must ensure that the conditions of their
work do not create any hazard for those working
near by;
Laboratory coats should be fully buttoned up
while working and removed before leaving the
laboratory.
18. Contd…
The chances of an accident occurring in the
laboratory are much reduced if:
o Every one works in a tidy fashion;
o Every one works with out rush;
o Benches are clean;
o Reagents returned to the shelves after each use;
o No eating, drinking or smoking in the laboratory.
N.B: Eat, drink and be merry but not in the
laboratory!
19.
20. General precautions for avoidance
of laboratory accidents:
Handle acids and alkalis firmly up right with a dry
hand. Never pour water in to strong acids
because of the danger of reaction and splashing.
Do not keep acids and alkalis in bottles with
ground glass stoppers. This is because they may
get stuck.
Use small measuring cylinders for measuring
acids and alkalis where possible.
Use pipettes that are plugged with non-
absorbent cotton wool or with a rubber tube
attachment.
21. Contd…
Never heat the bottom of a test tube. The liquid inside
might sputter. Always heat the middle portion of the
test tube, shaking gently. The mouthpart of the test
tube should be facing away from the worker and other
person.
Only boro - silicate glass wares can be heated over a
Bunsen flame.
Only small quantities of inflammable liquids should
be kept in the lab.
Caution: Ether ignites at a distance of several meters
from a flame. Hence, never place a bottle of ether on
a workbench where there is an open flame (Bunsen
burner, sprite lamp, etc.)
22. Contd…
When lighting a butane gas (gas burner) always
light the match and hold it to the burner before
turning on the gas tap. Turn off the main valves of
all bottles of butane gas every evening.
Eating, drinking and smoking in the laboratory
should be strictly forbidden.
Students and junior staff must be aware of the
dangers of unauthorized experiments.
Safe disposal of specimens and contaminated
materials is highly essential.
Safe use and storage of different lab chemicals is
mandatory.
23. Contd…
Safe use of electrical equipment is of paramount
importance.
Adequately equipped first aid box should be
available in the laboratory
Dangerous laboratory chemicals and specimens
should be labeled clearly.
Laboratory equipments should be sterilized as
required;
Using of Biological Safety Cabinets when
appropriate.
24. Contd…
Biological Safety Cabinets are designed to
protect the laboratory personnel, the laboratory
environment and work materials from exposure to
infectious aerosols and splashes that may be
generated when manipulating materials
containing infectious agents, such as primary
cultures, stocks and diagnostic specimens.
N.B: It is extremely important to use gloves as a
personal means of protection from various
infectious agents while working in medical
laboratories.