Prokaryotic Gene Expression Asignment
•
1 like•403 views
This document outlines tasks for a unit on genetics and genetic engineering. It includes three tasks: 1) A presentation on current genetic engineering techniques and applications in industry; 2) A practical report on controlling gene expression of beta galactosidase in prokaryotes; 3) A poster and 1,000 word essay comparing prokaryotic and eukaryotic cells for genetic engineering. The goals are to explain prokaryotic gene expression control, demonstrate understanding of the lac operon, and explain why prokaryotes are preferred over eukaryotes for genetic engineering. The tasks will be assessed to achieve a pass, merit, or distinction grade.
Report
Share
Report
Share
Download to read offline
Recommended
Expressed sequence tag (EST), molecular marker
This document discusses expressed sequence tags (ESTs), which are short sequences of cDNA used to identify genes and study gene expression. It provides a brief history of ESTs, noting they were first coined in 1991. ESTs are generated by sequencing fragments of cDNA from mRNA. They provide a quick and inexpensive way to discover new genes and study transcriptomes. Large databases of ESTs exist that can be searched and mined for various applications, including gene discovery, similarity searching, and transcriptome analysis. Pre-processing and clustering/assembling tools are used to improve EST data quality.
RNA-seq: A High-resolution View of the Transcriptome
The molecular microscopes that we use to examine human biology have advanced significantly with the advent of next generation sequencing. RNA-seq is one application of this technology that leads to a very high-resolution view of the transcriptome. With these new technologies come increased data analysis and data handling burdens as well as the promise of new discovery. These slides present a high-level overview of the RNA-seq technology with a focus on the analysis approaches, quality control challenges, and experimental design.
Iplant pag
1) The study aimed to improve the genome annotation of Brassica rapa by assembling its transcriptome using RNA-Seq data from multiple tissues and detecting novel transcripts.
2) Over 128 RNA-Seq libraries were generated, sequenced, and quality controlled using resources on the iPlant Collaborative and XSEDE. De novo and reference-based assembly was performed.
3) The analysis detected over 3,500 novel transcripts and updated the gene models for over 28,000 genes in the B. rapa genome. The hybrid assembly approach provided a more accurate annotation compared to single methods.
Functional genomics, a conceptual approach
Introduction
Definition
History
Goals of functional genomics
Functional genomics approach :
Reverse genetics
Forward genetics
Tools for forward and reverse genetics
Limitations
Conclusion
reference
Express sequence tags
ESTs are short sequences of DNA derived from cDNA clones that represent gene expression in particular cells or tissues. They provide a simple and inexpensive way to discover new genes and map their positions in genomes. To create an EST, mRNA is converted to cDNA and then sequenced, yielding short expressed DNA sequences. ESTs are deposited in public databases like NCBI's dbEST and can help identify genes, construct genome maps, and characterize expressed genes through clustering, assembly, and mapping to genomic sequences. However, isolating mRNA from some tissues can be difficult and ESTs alone do not indicate the genes they were derived from.
Molecular marker and application
Synopsis :-
Introduction {What is marker ?}
History
Types of marker
Categories of molecular markers
Applications
Conclusion
References
Est database
ESTs are short sequences of DNA that represent genes expressed in certain tissues or organisms. They provide a quick and inexpensive way for scientists to discover new genes and map their positions in genomes. ESTs represent a snapshot of genes expressed in a tissue at a given time. Sequencing the beginning or end of cDNA clones produces 5' and 3' ESTs, which can help identify genes and study gene expression and regulation.
BM405 Lecture Slides 21/11/2014 University of Strathclyde
Slides from lectures delivered at the University of Strathclyde, Glasgow, 21st November 2014, on the BM405 course.
Recommended
Expressed sequence tag (EST), molecular marker
This document discusses expressed sequence tags (ESTs), which are short sequences of cDNA used to identify genes and study gene expression. It provides a brief history of ESTs, noting they were first coined in 1991. ESTs are generated by sequencing fragments of cDNA from mRNA. They provide a quick and inexpensive way to discover new genes and study transcriptomes. Large databases of ESTs exist that can be searched and mined for various applications, including gene discovery, similarity searching, and transcriptome analysis. Pre-processing and clustering/assembling tools are used to improve EST data quality.
RNA-seq: A High-resolution View of the Transcriptome
The molecular microscopes that we use to examine human biology have advanced significantly with the advent of next generation sequencing. RNA-seq is one application of this technology that leads to a very high-resolution view of the transcriptome. With these new technologies come increased data analysis and data handling burdens as well as the promise of new discovery. These slides present a high-level overview of the RNA-seq technology with a focus on the analysis approaches, quality control challenges, and experimental design.
Iplant pag
1) The study aimed to improve the genome annotation of Brassica rapa by assembling its transcriptome using RNA-Seq data from multiple tissues and detecting novel transcripts.
2) Over 128 RNA-Seq libraries were generated, sequenced, and quality controlled using resources on the iPlant Collaborative and XSEDE. De novo and reference-based assembly was performed.
3) The analysis detected over 3,500 novel transcripts and updated the gene models for over 28,000 genes in the B. rapa genome. The hybrid assembly approach provided a more accurate annotation compared to single methods.
Functional genomics, a conceptual approach
Introduction
Definition
History
Goals of functional genomics
Functional genomics approach :
Reverse genetics
Forward genetics
Tools for forward and reverse genetics
Limitations
Conclusion
reference
Express sequence tags
ESTs are short sequences of DNA derived from cDNA clones that represent gene expression in particular cells or tissues. They provide a simple and inexpensive way to discover new genes and map their positions in genomes. To create an EST, mRNA is converted to cDNA and then sequenced, yielding short expressed DNA sequences. ESTs are deposited in public databases like NCBI's dbEST and can help identify genes, construct genome maps, and characterize expressed genes through clustering, assembly, and mapping to genomic sequences. However, isolating mRNA from some tissues can be difficult and ESTs alone do not indicate the genes they were derived from.
Molecular marker and application
Synopsis :-
Introduction {What is marker ?}
History
Types of marker
Categories of molecular markers
Applications
Conclusion
References
Est database
ESTs are short sequences of DNA that represent genes expressed in certain tissues or organisms. They provide a quick and inexpensive way for scientists to discover new genes and map their positions in genomes. ESTs represent a snapshot of genes expressed in a tissue at a given time. Sequencing the beginning or end of cDNA clones produces 5' and 3' ESTs, which can help identify genes and study gene expression and regulation.
BM405 Lecture Slides 21/11/2014 University of Strathclyde
Slides from lectures delivered at the University of Strathclyde, Glasgow, 21st November 2014, on the BM405 course.
Gene expression profiling i
The document summarizes various unsupervised learning algorithms used for analyzing gene expression data from microarray experiments, including k-means clustering, self-organizing maps, and hierarchical clustering. It describes how these algorithms group genes based on similarity in their expression profiles across different conditions or cell types without external labels, helping to simplify data sets and identify genes that may be co-regulated or serve similar functions.
BIOL335: How to annotate a genome
This document discusses various methods for annotating genomes after sequencing and assembly. Sequence analysis approaches like identifying open reading frames can rapidly and inexpensively find some genes, but have weaknesses like false positives and missing short genes. More accurate methods are needed to find non-coding RNAs, pseudogenes, and other elements. As sequencing technologies generate more data, the bottleneck has shifted to analysis, requiring skills in both biology and mathematics. The document provides an example sequence to annotate and poses questions about fast, cheap and accurate annotation methods.
Molecular taxonomy
Taxonomy is the branch of science concerned with the classification of organisms. A taxonomic designation is more than just a name. Ideally, it reflects evolutionary history and the relationship between organisms. Traditionally, taxonomic classification has relied upon morphological features and physiological characteristics. However, for bacterial taxonomy, phenotypic approaches have proven insufficient. Unrelated bacteria can exhibit identical traits, closely related bacteria can have divergent features, and methods for accurate identification may be too cumbersome for routine use. In contrast, molecular taxonomy approaches use data derived from hereditary material and provide a robust view of genetic relatedness. Advances in technology have been accompanied by improvements in the cost, speed, and availability of molecular methods. Here, we provide a brief history of approaches to prokaryotic classification and describe how molecular taxonomy is redefining our understanding of bacterial evolution and the tree of life.
Serial analysis of gene expression
Serial Analysis of Gene Expression (SAGE) is a method that allows for the quantitative analysis of gene expression patterns in cells or tissues without prior knowledge of gene sequences. It works by extracting short sequence tags (10-14 base pairs) from the 3' end of transcripts and linking them together in long strings that can be cloned and sequenced simultaneously. This allows for many transcripts to be analyzed from a single sequencing reaction and provides quantitative data on gene expression levels based on tag frequencies. SAGE provides an accurate way to discover genes and analyze overall expression patterns without needing to know full mRNA sequences in advance.
Bioinf4 genedisco
Some tools and bioinformatics concepts useful to guide gene discovery associated to traits of interest for biotechnology applications.
Functional genomics
Functional genomics uses genome-wide experimental approaches to assess gene function on a large scale. It analyzes gene expression through techniques like transcriptomics and proteomics. Transcriptomics analyzes gene expression profiles through RNA sequencing or microarray analysis. Microarray analysis involves hybridizing fluorescently-labeled cDNA or cRNA to microarrays containing DNA probes to measure gene expression levels across thousands of genes simultaneously. Functional genomics provides a global understanding of gene function and molecular interactions through integrated omics approaches.
Transcriptome Analysis & Applications
High throughput next generation sequencing and robust transcriptome analysis help with gene expression profiling, gene annotation or discovery of non-coding RNA.
Sage
SAGE is a technique that allows for the digital analysis of overall gene expression patterns through the use of short sequence tags to uniquely identify transcripts without requiring preexisting clones. It works by linking these tags together into long serial molecules that can then be cloned and sequenced, with the number of times a particular tag is observed providing the expression level of the corresponding transcript. This allows for rapid sequencing analysis of multiple transcripts from a single sequencing event. SAGE is useful for comparative expression studies to identify differences in gene expression between tissues.
DNA AND RNA BASED TAXONOMY OF ANIMALS
This document discusses molecular taxonomy and DNA/RNA-based taxonomy of animals. Molecular techniques like DNA and protein sequencing, immunological methods, and DNA-DNA or DNA-RNA hybridization are used to establish genetic relationships between taxa and construct phylogenetic trees. DNA is extracted from specimens and specific genes are analyzed to find base sequences, which are then compared to standard sequences to identify species. RNA classification also helps with taxonomy, examining messenger RNA, ribosomal RNA, transfer RNA, and smaller nuclear RNA.
Differential gene profiling methods
This document discusses differential gene profiling methods, specifically differential display and subtractive hybridization. It provides details on how differential display and subtractive hybridization work, including that differential display allows comparison of gene expression between two samples and was commonly used in the 1990s before being replaced by microarrays in 2000. Subtractive hybridization identifies differences in nucleic acids between two populations and enriches for sequences not common to both. The document discusses applications and references for further information.
Microarray profiling by ujjwal sirohi
DNA microarrays are solid supports with organized grids of DNA probes that represent single genes. They use hybridization of fluorescent targets to probes to measure gene expression. Key steps involve sample collection, RNA isolation, fluorescent target labeling, hybridization to the array, washing, and scanning to analyze gene expression levels. Microarrays can be used for applications like gene expression profiling, disease diagnosis, and response to environmental changes. However, they have drawbacks like decreased specificity of probes and challenges with RNA quality and quantity from samples.
Biotech 2012 spring-7_-rflp_0
Genetic testing uses methods like RFLP analysis and DNA typing to analyze mutations associated with diseases. RFLP analysis involves detecting changes in restriction enzyme sites that can indicate different gene variants linked to diseases. DNA typing uses analysis of microsatellite repeats and VNTR sequences, which are highly variable between individuals, to identify genetic differences for applications like paternity testing and forensic analysis. The cystic fibrosis gene was located through a process of chromosome walking that used RFLP markers and linkage analysis to progressively narrow down the region of chromosome 7 containing the gene.
Enhanced structural variant and breakpoint detection using SVMerge by integra...
This document describes SVMerge, a meta structural variant calling pipeline that integrates multiple SV detection methods and uses local assembly to validate calls. It summarizes SVMerge's workflow of running various SV callers individually on BAM files, merging and validating calls using local de novo assembly. The document also provides examples of how SVMerge was applied to a HapMap trio dataset, demonstrating it discovered more SVs than individual callers and had a low false positive rate compared to a curated database. Future work areas are identified like cataloging complex SV events and improving heterozygous call support.
Biological databases
What are biological data bases, what are its types and how do they work everything is explained in these slides
Transcriptomics approaches
The study of the complete set of RNAs (transcriptome) encoded by the genome of a specific cell or organism at a specific time or under a specific set of conditions is called Transcriptomics.
Transcriptomics aims:
I. To catalogue all species of transcripts, including mRNAs, noncoding RNAs and small RNAs.
II. To determine the transcriptional structure of genes, in terms of their start sites, 5′ and 3′ ends, splicing patterns and other post-transcriptional modifications.
III. To quantify the changing expression levels of each transcript during development and under different conditions.
Microarray @ujjwal sirohi
A DNA microarray (also commonly known as DNA chip or biochip) is a collection of microscopic DNA spots attached to a solid surface.
The core principle behind microarrays is hybridization between two DNA strands, the property of complementary nucleic acid sequences to specifically pair with each other by forming hydrogen bonds between complementary nucleotide base pairs.
GBS: Genotyping by sequencing
Genotyping-by-Sequencing (GBS): A Novel, Efficient and Cost-Effective Genotyping method Using Next-Generation Sequencing
Microarray
Microarrays are tools used to analyze gene expression. They consist of small glass slides containing samples of many genes arranged in a regular pattern. The genes are used to detect mRNA species present in a cell or tissue at a given time, allowing analysis of which genes are actively being expressed. Major uses of microarrays include analysis of gene expression, single nucleotide polymorphisms, and identification of pathogens or tumors. They provide a way to detect differences in gene expression across large numbers of genes in different cell populations on a genome-wide scale.
MASSIVELY PARELLEL SIGNATURE SEQUENCING
MPSS is a technique for analyzing gene expression that involves sequencing cDNA fragments cloned onto microbeads. It allows for the simultaneous sequencing of over 1 million cDNA clones. MPSS generates 17-base signature sequences that uniquely identify mRNA transcripts. Gene expression levels are quantified by counting the number of signatures for each gene. MPSS provides a more in-depth analysis of gene expression compared to other methods as it can detect genes expressed at very low levels and does not require prior knowledge of gene sequences.
Multilocus sequence typin1
Multilocus sequence typing (MLST) is a technique used to characterize microbes based on DNA sequences of fragments of multiple housekeeping genes. For each gene, different sequences are assigned as distinct alleles, and the combination of alleles across genes defines the sequence type (ST) of a strain. MLST provides an unambiguous and portable method for classifying strains that can be used for evolutionary and epidemiological studies. It has been applied to characterize many human and plant pathogens. While powerful, MLST has limitations such as potentially lacking discriminatory power and neglecting non-housekeeping genes.
Gene Expression & regulation
Gene expression is regulated in both prokaryotes and eukaryotes. In prokaryotes, operons including promoters, operators, and structural genes turn genes on and off by controlling transcription. The lac operon acts like a switch that is off when lactose is absent and on when lactose is present. In eukaryotes, gene expression is regulated at transcription through regulatory DNA sequences and transcription factors, and through RNA processing which splices exons, adds a cap and tail to mRNA.
Gene expression in prokaryotes
Regulated gene expression is required for adaptation, differentiation, and development in organisms. In prokaryotes, genes involved in metabolic pathways are often arranged in operons, where a single regulatory region controls multiple structural genes. The lac operon in E. coli regulates genes for lactose metabolism. In the absence of lactose, the lac repressor binds the operator region and prevents transcription. When lactose is present, it binds the repressor and induces a conformational change that reduces its affinity for DNA, allowing transcription. This is an example of negative regulation through repression and derepression.
More Related Content
What's hot
Gene expression profiling i
The document summarizes various unsupervised learning algorithms used for analyzing gene expression data from microarray experiments, including k-means clustering, self-organizing maps, and hierarchical clustering. It describes how these algorithms group genes based on similarity in their expression profiles across different conditions or cell types without external labels, helping to simplify data sets and identify genes that may be co-regulated or serve similar functions.
BIOL335: How to annotate a genome
This document discusses various methods for annotating genomes after sequencing and assembly. Sequence analysis approaches like identifying open reading frames can rapidly and inexpensively find some genes, but have weaknesses like false positives and missing short genes. More accurate methods are needed to find non-coding RNAs, pseudogenes, and other elements. As sequencing technologies generate more data, the bottleneck has shifted to analysis, requiring skills in both biology and mathematics. The document provides an example sequence to annotate and poses questions about fast, cheap and accurate annotation methods.
Molecular taxonomy
Taxonomy is the branch of science concerned with the classification of organisms. A taxonomic designation is more than just a name. Ideally, it reflects evolutionary history and the relationship between organisms. Traditionally, taxonomic classification has relied upon morphological features and physiological characteristics. However, for bacterial taxonomy, phenotypic approaches have proven insufficient. Unrelated bacteria can exhibit identical traits, closely related bacteria can have divergent features, and methods for accurate identification may be too cumbersome for routine use. In contrast, molecular taxonomy approaches use data derived from hereditary material and provide a robust view of genetic relatedness. Advances in technology have been accompanied by improvements in the cost, speed, and availability of molecular methods. Here, we provide a brief history of approaches to prokaryotic classification and describe how molecular taxonomy is redefining our understanding of bacterial evolution and the tree of life.
Serial analysis of gene expression
Serial Analysis of Gene Expression (SAGE) is a method that allows for the quantitative analysis of gene expression patterns in cells or tissues without prior knowledge of gene sequences. It works by extracting short sequence tags (10-14 base pairs) from the 3' end of transcripts and linking them together in long strings that can be cloned and sequenced simultaneously. This allows for many transcripts to be analyzed from a single sequencing reaction and provides quantitative data on gene expression levels based on tag frequencies. SAGE provides an accurate way to discover genes and analyze overall expression patterns without needing to know full mRNA sequences in advance.
Bioinf4 genedisco
Some tools and bioinformatics concepts useful to guide gene discovery associated to traits of interest for biotechnology applications.
Functional genomics
Functional genomics uses genome-wide experimental approaches to assess gene function on a large scale. It analyzes gene expression through techniques like transcriptomics and proteomics. Transcriptomics analyzes gene expression profiles through RNA sequencing or microarray analysis. Microarray analysis involves hybridizing fluorescently-labeled cDNA or cRNA to microarrays containing DNA probes to measure gene expression levels across thousands of genes simultaneously. Functional genomics provides a global understanding of gene function and molecular interactions through integrated omics approaches.
Transcriptome Analysis & Applications
High throughput next generation sequencing and robust transcriptome analysis help with gene expression profiling, gene annotation or discovery of non-coding RNA.
Sage
SAGE is a technique that allows for the digital analysis of overall gene expression patterns through the use of short sequence tags to uniquely identify transcripts without requiring preexisting clones. It works by linking these tags together into long serial molecules that can then be cloned and sequenced, with the number of times a particular tag is observed providing the expression level of the corresponding transcript. This allows for rapid sequencing analysis of multiple transcripts from a single sequencing event. SAGE is useful for comparative expression studies to identify differences in gene expression between tissues.
DNA AND RNA BASED TAXONOMY OF ANIMALS
This document discusses molecular taxonomy and DNA/RNA-based taxonomy of animals. Molecular techniques like DNA and protein sequencing, immunological methods, and DNA-DNA or DNA-RNA hybridization are used to establish genetic relationships between taxa and construct phylogenetic trees. DNA is extracted from specimens and specific genes are analyzed to find base sequences, which are then compared to standard sequences to identify species. RNA classification also helps with taxonomy, examining messenger RNA, ribosomal RNA, transfer RNA, and smaller nuclear RNA.
Differential gene profiling methods
This document discusses differential gene profiling methods, specifically differential display and subtractive hybridization. It provides details on how differential display and subtractive hybridization work, including that differential display allows comparison of gene expression between two samples and was commonly used in the 1990s before being replaced by microarrays in 2000. Subtractive hybridization identifies differences in nucleic acids between two populations and enriches for sequences not common to both. The document discusses applications and references for further information.
Microarray profiling by ujjwal sirohi
DNA microarrays are solid supports with organized grids of DNA probes that represent single genes. They use hybridization of fluorescent targets to probes to measure gene expression. Key steps involve sample collection, RNA isolation, fluorescent target labeling, hybridization to the array, washing, and scanning to analyze gene expression levels. Microarrays can be used for applications like gene expression profiling, disease diagnosis, and response to environmental changes. However, they have drawbacks like decreased specificity of probes and challenges with RNA quality and quantity from samples.
Biotech 2012 spring-7_-rflp_0
Genetic testing uses methods like RFLP analysis and DNA typing to analyze mutations associated with diseases. RFLP analysis involves detecting changes in restriction enzyme sites that can indicate different gene variants linked to diseases. DNA typing uses analysis of microsatellite repeats and VNTR sequences, which are highly variable between individuals, to identify genetic differences for applications like paternity testing and forensic analysis. The cystic fibrosis gene was located through a process of chromosome walking that used RFLP markers and linkage analysis to progressively narrow down the region of chromosome 7 containing the gene.
Enhanced structural variant and breakpoint detection using SVMerge by integra...
This document describes SVMerge, a meta structural variant calling pipeline that integrates multiple SV detection methods and uses local assembly to validate calls. It summarizes SVMerge's workflow of running various SV callers individually on BAM files, merging and validating calls using local de novo assembly. The document also provides examples of how SVMerge was applied to a HapMap trio dataset, demonstrating it discovered more SVs than individual callers and had a low false positive rate compared to a curated database. Future work areas are identified like cataloging complex SV events and improving heterozygous call support.
Biological databases
What are biological data bases, what are its types and how do they work everything is explained in these slides
Transcriptomics approaches
The study of the complete set of RNAs (transcriptome) encoded by the genome of a specific cell or organism at a specific time or under a specific set of conditions is called Transcriptomics.
Transcriptomics aims:
I. To catalogue all species of transcripts, including mRNAs, noncoding RNAs and small RNAs.
II. To determine the transcriptional structure of genes, in terms of their start sites, 5′ and 3′ ends, splicing patterns and other post-transcriptional modifications.
III. To quantify the changing expression levels of each transcript during development and under different conditions.
Microarray @ujjwal sirohi
A DNA microarray (also commonly known as DNA chip or biochip) is a collection of microscopic DNA spots attached to a solid surface.
The core principle behind microarrays is hybridization between two DNA strands, the property of complementary nucleic acid sequences to specifically pair with each other by forming hydrogen bonds between complementary nucleotide base pairs.
GBS: Genotyping by sequencing
Genotyping-by-Sequencing (GBS): A Novel, Efficient and Cost-Effective Genotyping method Using Next-Generation Sequencing
Microarray
Microarrays are tools used to analyze gene expression. They consist of small glass slides containing samples of many genes arranged in a regular pattern. The genes are used to detect mRNA species present in a cell or tissue at a given time, allowing analysis of which genes are actively being expressed. Major uses of microarrays include analysis of gene expression, single nucleotide polymorphisms, and identification of pathogens or tumors. They provide a way to detect differences in gene expression across large numbers of genes in different cell populations on a genome-wide scale.
MASSIVELY PARELLEL SIGNATURE SEQUENCING
MPSS is a technique for analyzing gene expression that involves sequencing cDNA fragments cloned onto microbeads. It allows for the simultaneous sequencing of over 1 million cDNA clones. MPSS generates 17-base signature sequences that uniquely identify mRNA transcripts. Gene expression levels are quantified by counting the number of signatures for each gene. MPSS provides a more in-depth analysis of gene expression compared to other methods as it can detect genes expressed at very low levels and does not require prior knowledge of gene sequences.
Multilocus sequence typin1
Multilocus sequence typing (MLST) is a technique used to characterize microbes based on DNA sequences of fragments of multiple housekeeping genes. For each gene, different sequences are assigned as distinct alleles, and the combination of alleles across genes defines the sequence type (ST) of a strain. MLST provides an unambiguous and portable method for classifying strains that can be used for evolutionary and epidemiological studies. It has been applied to characterize many human and plant pathogens. While powerful, MLST has limitations such as potentially lacking discriminatory power and neglecting non-housekeeping genes.
What's hot (20)
Enhanced structural variant and breakpoint detection using SVMerge by integra...
Enhanced structural variant and breakpoint detection using SVMerge by integra...
Viewers also liked
Gene Expression & regulation
Gene expression is regulated in both prokaryotes and eukaryotes. In prokaryotes, operons including promoters, operators, and structural genes turn genes on and off by controlling transcription. The lac operon acts like a switch that is off when lactose is absent and on when lactose is present. In eukaryotes, gene expression is regulated at transcription through regulatory DNA sequences and transcription factors, and through RNA processing which splices exons, adds a cap and tail to mRNA.
Gene expression in prokaryotes
Regulated gene expression is required for adaptation, differentiation, and development in organisms. In prokaryotes, genes involved in metabolic pathways are often arranged in operons, where a single regulatory region controls multiple structural genes. The lac operon in E. coli regulates genes for lactose metabolism. In the absence of lactose, the lac repressor binds the operator region and prevents transcription. When lactose is present, it binds the repressor and induces a conformational change that reduces its affinity for DNA, allowing transcription. This is an example of negative regulation through repression and derepression.
Microbial Genetics
Microbial genetics is a subject area within microbiology and genetic engineering. This involves the study of the genotype of microbial species and also the expression system in the form of phenotypes
Mutations
Mutations are changes in an organism's DNA that may or may not affect phenotype. A mutation can involve a single nucleotide substitution or the insertion or deletion of nucleotides, and can occur in a single gene or across entire chromosomes. Mutations can have varying effects depending on their location and type, from no observable effect to large impacts on phenotype. They can arise from replication errors or exposure to mutagens like UV radiation or chemicals.
Microbial genetics lectures 19,20, and 21
This document discusses plasmids and transposons in bacteria. It explains that plasmids are small, circular pieces of DNA separate from the bacterial chromosome that can contain genes enhancing survival. Plasmids can transfer genes horizontally through conjugation. Transposons are mobile DNA elements that can move locations within a genome, sometimes carrying antibiotic resistance genes. They include insertion sequences and larger transposons, which may contain resistance genes flanked by insertion sequences that allow for transposition. Together, plasmids and transposons facilitate horizontal gene transfer and generation of genetic diversity in bacteria.
Overview of transcription
Overview of transcriptionMarudhar Kesari Jain College for Women Vaniyambadi - 635 751, Tamil Nadu, INDIA.
Transcription - prokaryotic & Eukaryotic, transcription regulatory elements, inhibitors of TranscriptionTranscription and translation
Prokaryotic transcription involves RNA polymerase binding to promoter sequences on DNA and synthesizing RNA without the need for primers. It proceeds through initiation, elongation, and termination stages. Eukaryotic transcription is more complex, utilizing three RNA polymerases and involving transcription factors, mediator complexes, 5' capping, splicing, and 3' polyadenylation to process mRNA. Alternative splicing allows single genes to code for multiple proteins through different combinations of exons.
Transcription
The document discusses transcription in prokaryotes and eukaryotes. In prokaryotes, RNA polymerase binds to promoter sequences and transcribes DNA into RNA through initiation, elongation, and termination. Transcription requires RNA polymerase and proceeds similarly in eukaryotes but involves multiple RNA polymerases and occurs in the nucleus. Eukaryotic transcription is more complex, utilizing regulatory sequences, transcription factors, and RNA processing to modify pre-mRNA into mature mRNA through splicing, capping, polyadenylation, and other modifications. Mutations can affect splicing and cause genetic disorders like beta-thalassemia.
Microbial genetics lectures 1, 2, and 3
1. The document discusses the structure and function of DNA, RNA, and viral genomes. It explains that DNA contains genes which code for RNA and proteins, while RNA has different types that aid in protein production.
2. The key differences between DNA and RNA are that DNA is double-stranded, more stable, and stores genetic information, while RNA is single-stranded and acts as a messenger between DNA and ribosomes.
3. Viruses contain either DNA or RNA as their genetic material within a protein coat, and vary in genome size and structure between linear, circular, single-stranded and double-stranded forms.
Phage stratagies
The document summarizes key aspects of bacteriophage lambda. It describes that lambda can enter either the lytic cycle or lysogenic cycle. The lytic cycle involves a cascade of gene expression controlled by regulators that ultimately leads to bacterial lysis and phage particle release. The lysogenic cycle is maintained by the lambda repressor protein, which binds as a dimer to three operator sites and prevents expression of genes needed for the lytic cycle. The repressor uses a helix-turn-helix motif for specific DNA binding and binds cooperatively to operators to maintain lysogeny through an autoregulatory circuit.
TRANSPOSONS
This document discusses transposons, also known as transposable elements or jumping genes. Transposons are DNA sequences that can change their position within the genome, sometimes causing or reversing mutations. The document outlines the mechanisms by which transposable elements are regulated in germ cells and somatic cells, and how their insertions can contribute to genetic variation and disease. It also discusses how transposable element insertions have been linked to various genetic disorders and cancers. The conclusion estimates that around 700 novel transposable element insertion events occur in a single diploid human genome.
Transposons ask
This document provides an overview of transposable elements (TEs). It discusses that TEs are DNA sequences that can move within genomes. There are two major classes of TEs - retrotransposons which move via an RNA intermediate, and DNA transposons which move directly through a cut-and-paste or copy-and-paste mechanism. The document outlines different types of TEs found in bacteria, eukaryotes, and the human genome. It also discusses the impact of TEs in causing mutations and their applications in genetic engineering and mutagenesis experiments.
281 lec13 prokaryotic_transcription
This document discusses transcription in prokaryotes. It begins by outlining the aims of understanding the transcription process, gene structure, promoter and terminator structures, and how transcription is terminated. The transcription process involves three steps - initiation, elongation, and termination. Initiation occurs at the promoter region, which contains -10 and -35 boxes. Elongation involves RNA polymerase moving along the DNA and synthesizing RNA. Termination can occur via Rho-independent terminators that form hairpin loops, or Rho-dependent terminators involving the Rho protein. The gene structure contains a promoter region, RNA coding sequence, and terminator region.
281 lec18 prokaryotic_translation
1. Translation in prokaryotes begins with the small 30S ribosomal subunit binding to the Shine-Dalgarno sequence in mRNA with initiation factors IF1 and IF3.
2. The initiator tRNA carrying formyl-methionine then binds to the start codon with help from IF2.
3. The large 50S subunit joins to form the 70S initiation complex.
4. Elongation adds amino acids to the growing polypeptide chain through the actions of charged tRNAs, elongation factors, and peptidyl transferase moving the ribosome downstream codon by codon.
5. Termination occurs when a stop codon is reached and release factors cause the
Lamda phage
Lambda phage is a bacteriophage that infects E. coli bacteria. It has two life cycles: a lytic cycle and a lysogenic cycle. In the lytic cycle, the phage genome is transferred into the bacterial cell where it replicates and causes the bacterial cell to burst, releasing new phage particles. In the lysogenic cycle, the phage genome integrates into the bacterial chromosome and replicates with the host DNA without killing the cell. The phage can switch between these two cycles depending on environmental conditions inside the infected bacterial cell.
Transcription and translation lecture notes
BIo cells and molecules 1200 course notes on transcription and translation process in Eukaryotes and prokaryotic cells
(2) microbial genetics
The document discusses several key aspects of microbial genetics including:
1) Microbial genetics revealed that genes consist of DNA and led to the discovery of restriction enzymes and PCR for amplifying specific DNA regions.
2) Prokaryotic genomes typically consist of a single circular DNA molecule along with extrachromosomal genetic elements like plasmids and bacteriophages.
3) Viruses depend on host cells for replication and require a mechanism for invading hosts and packaging viral components to infect new hosts. Bacteriophages can integrate into host genomes or cause lytic infections.
Rna synthesis
The central dogma of molecular biology proposes a one-way flow of information from DNA to RNA to protein. Transcription involves RNA polymerase making RNA using DNA as a template in the 3' to 5' direction. The three major types of RNA are mRNA, rRNA, and tRNA. Elongation of transcription occurs as RNA polymerase adds nucleotides to the growing RNA strand. Termination happens via different mechanisms depending on the type of RNA polymerase and results in release of the completed RNA molecule.
Prokaryotic transcription
Prokaryotic transcriptionMarudhar Kesari Jain College for Women Vaniyambadi - 635 751, Tamil Nadu, INDIA.
Transcription in prokaryotes involves RNA polymerase producing messenger RNA transcripts of genetic material in the cytoplasm. Unlike in eukaryotes, transcription and translation can occur simultaneously. Transcription is controlled by transcription factors and involves three main steps: initiation, elongation, and termination. Initiation requires RNA polymerase binding to promoter regions with sigma factors. Elongation adds complementary bases to the DNA template. Termination can occur via intrinsic terminator sequences forming stem-loop structures or with rho-dependent termination using a rho factor protein.Bacteriophage
Bacteriophages are viruses that infect bacteria. They were discovered in the early 20th century and come in diverse structural forms. Bacteriophages have a nucleic acid core surrounded by a protein coat. They undergo either a lytic cycle that results in host cell lysis or a lysogenic cycle where the phage DNA integrates into the host chromosome. The lysogenic cycle can confer new properties on the host bacteria through lysogenic conversion. Bacteriophages play important roles in bacterial evolution, epidemiology, and have applications in genetic engineering and controlling bacterial growth.
Viewers also liked (20)
Similar to Prokaryotic Gene Expression Asignment
Btec National Unit 18 Genetic Engineering
This document outlines an assignment on genetic engineering. Students are asked to research and describe two examples of genetic engineering - one related to crop production and one to medical science. They will create a front page covering the basic scientific principles and then profiles of each example, describing what was altered and the rationale. Students must also explain the commercial, social, and ethical concerns of each example. Finally, students must evaluate the benefits and drawbacks of using genetic engineering for each application. The assignment is assessed based on criteria for a pass, merit, and distinction grade.
Y13 Biotech L Os
This document outlines the learning outcomes for a unit on biotechnological techniques. It describes several key techniques including restriction enzymes, DNA ligase, polymerase chain reaction, gene therapy, gel electrophoresis, and DNA sequencing. It also covers applications such as plant tissue culture, animal cloning, gene cloning, transgenic organisms, DNA profiling, and genome analysis. The overall goal is for students to understand how these techniques meet human needs and demands.
PROKARYOTIC TRANSCRIPTOMICS AND METAGENOMICS
After billions of years of evolution, prokaryotes have developed a huge diversity of regulatory mechanisms, many of which are probably uncharacterized. Now that the powerful tool of whole-transcriptome analysis can be used to study the RNA of bacteria and archaea, a new set of un expected RNA-based regulatory strategies might be revealed.
Metagenomics, together with in vitro evolution and high-throughput screening technologies, provides industry with an unprecedented chance to bring biomolecules into industrial application.
Web Apollo: Lessons learned from community-based biocuration efforts.
This presentation tries to highlight the importance and relevance of community-based curation of biological data. It describes the results of harvesting expertise from dispersed researchers assigning functions to predicted and curated peptides, as well as collaborative efforts for standardization of genes and gene product attributes across species and databases.
Lab assignment 1 revised 2
The document provides instructions and templates for laboratory writing assignments as part of the RISE Program at the University of Puerto Rico - Cayey. It details 3 workshops that students participated in to learn various laboratory techniques. The first workshop covered microscopy techniques including bright field, dark field, and phase contrast microscopy as well as photomicrography. The second workshop involved using micropipettes, performing aseptic techniques, and working with bacteria. The third workshop over 3 days taught techniques in DNA isolation, polymerase chain reaction, electrophoresis, protein separation, and detection of oncogenes and oncoproteins. The document emphasizes that mastery of these laboratory techniques is important for scientific discovery and diagnostic applications in
Web Apollo Workshop University of Exeter
This presentation is a thorough guide to the use of Web Apollo, with details on User Navigation, Functionality, and the thought process behind manual annotation.
During this workshop, participants:
- Learn to identify homologs of known genes of interest in a newly sequenced genome.
- Become familiar with the environment and functionality of the Web Apollo genome annotation editing tool.
- Learn how to corroborate or modify automatically annotated gene models using available evidence in Web Apollo.
- Understand the process of curation in the context of genome annotation.
Btec National Unit 18 Dna Technology
The document outlines an assignment on genetic engineering and DNA technology. It discusses examining the techniques of genetic engineering, manipulating DNA, and investigative opportunities with scientific techniques. Students will research and describe basic DNA techniques like extraction, electrophoresis, PCR, and cell transformation. They will write lab manuals for two procedures and explain the reasons for key steps in each technique. Finally, students will discuss practical limitations of each technique and how they can be overcome.
Btec National Unit 13 From Dna To Protein
The document outlines an assignment for a laboratory technician to create an information packet explaining how DNA codes for proteins. It discusses three tasks: [1] creating fact sheets about DNA structure and genetic code; [2] explaining transcription and translation through a slidecast and RNA fact sheet; and [3] profiling each RNA type and relating structure to function in protein biosynthesis and industrial uses. The assignment provides evidence for passing, merit, and distinction grades, with distinction requiring relating RNA structure to roles in protein production and applications in industry.
Prototypes Of The Ovulona
The capability to anticipate ovulation well in advance, and to then detect ovulation independently of the predictive signals, is unique to the bioZhena technology. This unique capability results from the mode of action, further discussed in the Alphabet of bioZhena under Modus operandi (MO). http://biozhena.wordpress.com/2007/11/28/the-alphabet-of-biozhena
See also under Mysterious conceptions - or the non-existence thereof. From the MO also follows the broad applicability of the technology, which is another feature that distinguishes the Ovulona from any other product addressing fertility status and ovulation monitoring. For a potential impact of the technology on public health, see under Sexually transmitted diseases, and also under Cervical cancer and under Smoking.
It could be argued that the greatest aspect of the bioZhena project is the idea of introducing – via the affordable personal fertility monitoring method – a general, routinely usable, women’s health tracking and diagnostic tool, with the potential to impact on several important areas of public health.
Btec National Unit 13 Separating Biological Molecules Assignment Details
The document outlines an assignment for students to create fact sheets about the structure and function of proteins, carbohydrates, and lipids, including their properties that allow for separation from mixtures. Students will then discuss techniques that can be used to separate each of the molecules and include evidence that would show the separation was successful. The final task is for students to write a literature review on agarose gel electrophoresis, how it works to separate molecules, and attach an interpretation of electrophoresis results to determine band sizes in a sample. The purpose is to introduce medical biochemistry and molecular separation techniques. Completion of the tasks provides evidence that students can demonstrate and explain separation techniques, interpret experimental data, and evaluate the techniques used.
call for papers, research paper publishing, where to publish research paper, ...
call for papers, research paper publishing, where to publish research paper, ...International Journal of Engineering Inventions www.ijeijournal.com
1) The document describes a feature extraction program developed to analyze gene expression data from the Gene Expression Omnibus (GEO) database.
2) The program was tested on human transcription factor expression data sets and was able to successfully extract gene expression information.
3) Analyzing specific gene sets from GEO files had previously been a labor-intensive task, but the object-oriented program streamlines this process using C and Perl programming languages.Interpret gene expression results 2013
The document summarizes a webinar about using the GNC Pro software to analyze gene expression results from PCR array experiments. The webinar demonstrates how to input gene lists into GNC Pro, walk around the gene interaction network to find new candidate genes, verify gene interactions, check tissue-specific expression, and export results. It also discusses upcoming features like canonical pathways and color-coded fold changes. The webinar uses a case study analyzing gene expression changes in human PBMCs stimulated with phorbol ester and ionomycin to demonstrate the software.
rheumatoid arthritis
This document provides an overview of bioinformatics and genomics. It begins with an acknowledgement and abstract section. The introduction defines bioinformatics and its role in analyzing genetic sequences and biological data through computational methods. Major research areas of bioinformatics discussed include sequence analysis, genome annotation, evolutionary biology, measuring biodiversity, gene expression analysis, protein analysis, cancer mutation analysis, and protein structure prediction. Comparative genomics and modeling biological systems are also summarized. The document concludes with a definition of genomics as the study of genomes through sequencing efforts and mapping genetic interactions.
Processing Amplicon Sequence Data for the Analysis of Microbial Communities
This document provides an overview of next-generation sequencing (NGS) technologies and their usefulness for analyzing microorganisms associated with plants. It discusses how NGS methods allow addressing previously impossible questions about the composition, function, and interactions of microbial communities in environments like the rhizosphere and phyllosphere. While powerful, NGS platforms have limitations that can introduce errors or biases, but methods exist to overcome these issues. The review highlights applications of NGS in metagenomic studies of plant-associated microbiomes and how these new techniques are transforming the field.
one complete report from all the 4 labs.pdf
The document provides instructions for compiling a complete lab report from four biology labs on genomic databases, primer design, PCR, and molecular cloning. It outlines the necessary sections for the report, including an introduction describing the overall question and background, materials and methods, results with data and figures, and a discussion/conclusion section. It also provides additional details on designing a transgene reporter gene based on knowledge gained from the lab exercises, including defining a transgene, necessary gene elements, and ideas for using the transgene.
one complete report from all the 4 labs.pdf
The document provides instructions for compiling a complete lab report from four biology labs on genomic databases, primer design, PCR, and molecular cloning. It outlines the required sections of the report, including an introduction, materials and methods, results with data, and a discussion/conclusion section. It also provides discussion questions on building a reporter gene or transgene, defining key terms and outlining the necessary gene elements and ideas for using the transgene. The report should integrate results and instructions from all four labs.
Apollo Workshop at KSU 2015
Apollo is a web-based application that supports and enables collaborative genome curation in real time, allowing teams of curators to improve on existing automated gene models through an intuitive interface. Apollo allows researchers to break down large amounts of data into manageable portions to mobilize groups of researchers with shared interests.
Munoz torres web-apollo-workshop_exeter-2014_ss
This document provides an introduction and overview of a manual annotation workshop using the Web Apollo genome annotation tool. It discusses manual annotation and community-based curation efforts. The workshop aims to teach participants how to identify genes of interest, become familiar with Web Apollo, learn how to corroborate and modify gene models using evidence, and understand the genome annotation process from assembly to manual curation. The document outlines the workshop activities and provides guidance on using Web Apollo, including navigating the interface, editing annotations, and annotating simple cases by adding or modifying exons.
Chapter 20 ppt
This document summarizes key concepts from Chapter 20 of an AP Biology textbook. It discusses several topics:
1) Genomics is the study of genomes and how they are organized and regulated. Genome sequences provide insights into fundamental biological questions.
2) Computer analysis can identify protein-coding genes in DNA sequences by looking for start/stop signals and other features. With 25,000 genes in humans, this analysis is a huge undertaking without technology.
3) Genome sizes vary greatly between organisms, but size does not always correlate with complexity. Some plants have genomes much larger than humans despite fewer genes.
Similar to Prokaryotic Gene Expression Asignment (20)
Web Apollo: Lessons learned from community-based biocuration efforts.
Web Apollo: Lessons learned from community-based biocuration efforts.
Btec National Unit 13 Separating Biological Molecules Assignment Details
Btec National Unit 13 Separating Biological Molecules Assignment Details
call for papers, research paper publishing, where to publish research paper, ...
call for papers, research paper publishing, where to publish research paper, ...
Processing Amplicon Sequence Data for the Analysis of Microbial Communities
Processing Amplicon Sequence Data for the Analysis of Microbial Communities
More from Teresa Briercliffe
Extra Credit Exam Question Variation
An Australian study of 14,000 twin pairs sought to determine if there was a genetic basis for homosexuality. They focused on male identical twins. The researchers found that of the identical twin pairs where one twin was homosexual, 38% of the time the other twin was also homosexual. This suggests there may be a genetic influence for sexual orientation. However, the conclusion has limitations since other environmental factors could also influence sexual orientation.
Variation Lesson One
This 1 hour 30 minute lesson introduces students to the concept of variation within and between species. It aims to teach students key terms like genotype, phenotype, intraspecific and interspecific variation. The lesson will explore the genetic and environmental causes of variation and how variation can be measured on continuous or discontinuous scales. Students will consider examples of human traits that vary and sort them based on likely genetic or environmental influences. They will also discuss how confident we can be about stating single causes of variation, as influences are often complex.
Variation Lesson Two
Twins are used in variation studies because they allow researchers to isolate the effects of genetic and environmental factors. Studying twins who share genes but are raised in separate environments or together in the same environment can show whether differences between twins are due to nature or nurture. This helps researchers understand the relative contributions of genetic and environmental influences on traits.
Variation Is.
Variation exists between individuals of the same species due to both genetic and environmental factors. Genetic causes of variation include differences in DNA sequences between individuals, while environmental factors such as diet, climate, and other external influences can also impact variation. The document explores the sources and causes of variation between individuals.
Magnification Questions
This document contains practice biology questions about magnification and measurements of biological structures and cells. It includes images of a fish gill magnified 400x and asks the actual length of a filament. It also includes an image of a parasite spine magnified 1000x and asks the actual length. Finally, it includes drawings of a pancreatic cell measured at 8.6 micrometers and E. coli cells typically 2 micrometers long, asking the magnification of the images.
Biology Mock Exam
The document provides instructions for a biology mock exam to be taken at Haringey Sixth Form Centre Science Department. Students are to write their name and take a one hour exam with questions worth a total of 50 marks. Pencils, rulers, pens and calculators are required materials for the test.
Biology Homework Mark The Mock
This document contains an October biology mock exam paper for A. Student at Haringey Sixth Form Centre Science Department. The exam is one hour long and consists of multiple choice and short answer questions related to biology topics like enzymes, temperature effects on reaction rates, carbohydrates, and digestion. The questions assess understanding of key concepts and require explaining processes like hydrolysis, competitive inhibition, and the effects of enzyme denaturation.
Protein True And False
The document discusses proteins, stating that proteins are made of amino acids joined by peptide bonds, enzymes are proteins, antibodies are proteins, and there are 20 standard amino acids. Key proteins mentioned include keratin, actin, myosin, collagen, hemoglobin, and structural and transport proteins.
S
This document provides resources for investigating the effectiveness of methods to prevent the spread of swine flu, including petri dishes, agar, hand sanitizer, face masks, and bacteria samples. Students will work in small groups to plan an investigation with an hypothesis, risk assessment, and method section. Their assignment is to begin drafting an independent report with these planned sections and revising their introduction based on feedback.
Study Support Biohemical Molecules
This document provides guidance for creating a revision poster on biological molecules. It lists definitions, terminology, diagrams, monomers to polymers, and reactions that should be included. The poster must cover the structure of amino acids, glucose, and nucleotides as well as key terms like mono, di, tri, and poly. It will be reviewed and tested on October 9th to support an AS Biology class.
Swine Flu 1 Pdf
The document discusses swine flu and provides safety advice. It notes that antibiotics will not help with swine flu as it is a viral infection. It recommends wearing a safety harness if leaning out a window to avoid the risk of falling and injury.
Food Testing Carbohydrates
The document provides instructions for performing a series of tests to identify carbohydrates in food products. It describes preparing food samples, carrying out Benedict's test for reducing sugars, testing sensitivity of Benedict's test and Clinistix strips, and performing biochemical tests for non-reducing sugars and starch using Benedict's reagent and iodine solution. Observations are recorded and deductions made to identify which foods contain reducing sugars, non-reducing sugars, and starch.
Digestive System Label And Function
The human digestive system consists of the mouth, esophagus, stomach, small intestine, large intestine, and rectum. Food moves through this system where it is broken down and absorbed. The small intestine plays an important role in digestion through villi and microvilli that increase surface area for absorption. Glands like the salivary glands, pancreas, and liver secrete enzymes to further break down food into molecules that can be absorbed and used by the body.
Liz Claiborne Teen Dating Abuse Article
I. Incidences of teen dating abuse are unexpectedly high, with nearly 1 in 3 teens reporting sexual abuse, physical abuse, or threats of physical abuse, and nearly 1 in 2 reporting being controlled or pressured into unwanted acts. Many teens also know someone who has been abused.
II. There appears to be a link between families experiencing economic problems, higher levels of domestic violence between parents, and teens experiencing higher rates of dating abuse themselves.
III. Despite the high levels of abuse, most parents are dangerously unaware of the dating abuse occurring in their teens' lives.
Chd Case Study
Benny is a 54-year-old man whose lifestyle puts him at high risk for cardiovascular disease. He has a sedentary job and lifestyle aside from walking his dog once a day. His diet is unhealthy and high in cholesterol. He smokes 40 cigarettes per day. His father died of a heart attack in his late 40s, indicating a genetic risk factor.
Liz Claiborne Teen Dating Abuse Article
I. Incidences of teen dating abuse are unexpectedly high, with nearly 1 in 3 teens reporting sexual abuse, physical abuse, or threats of physical abuse, and nearly 1 in 2 reporting being controlled or pressured into unwanted acts. Many teens also know someone who has experienced abuse.
II. There appears to be a link between families experiencing economic problems, higher levels of domestic violence between parents, and teens experiencing higher rates of dating abuse themselves.
III. Despite the high levels of abuse, most parents are dangerously unaware of the dating abuse occurring in their teens' lives.
A2 And As Course Handbook
This document is a course handbook for AS and A2 Biology students at Haringey Sixth Form Centre. It provides information about staff roles, the course structure and units, assessment details, progression opportunities, expectations of students, a calendar of the academic year, study skills advice, health and safety policies, required equipment, and recommended reading materials and websites. The handbook is intended to give students all the necessary information to succeed in the course throughout their time at the Centre.
More from Teresa Briercliffe (20)
Recently uploaded
Artificial Intelligence for XMLDevelopment
In the rapidly evolving landscape of technologies, XML continues to play a vital role in structuring, storing, and transporting data across diverse systems. The recent advancements in artificial intelligence (AI) present new methodologies for enhancing XML development workflows, introducing efficiency, automation, and intelligent capabilities. This presentation will outline the scope and perspective of utilizing AI in XML development. The potential benefits and the possible pitfalls will be highlighted, providing a balanced view of the subject.
We will explore the capabilities of AI in understanding XML markup languages and autonomously creating structured XML content. Additionally, we will examine the capacity of AI to enrich plain text with appropriate XML markup. Practical examples and methodological guidelines will be provided to elucidate how AI can be effectively prompted to interpret and generate accurate XML markup.
Further emphasis will be placed on the role of AI in developing XSLT, or schemas such as XSD and Schematron. We will address the techniques and strategies adopted to create prompts for generating code, explaining code, or refactoring the code, and the results achieved.
The discussion will extend to how AI can be used to transform XML content. In particular, the focus will be on the use of AI XPath extension functions in XSLT, Schematron, Schematron Quick Fixes, or for XML content refactoring.
The presentation aims to deliver a comprehensive overview of AI usage in XML development, providing attendees with the necessary knowledge to make informed decisions. Whether you’re at the early stages of adopting AI or considering integrating it in advanced XML development, this presentation will cover all levels of expertise.
By highlighting the potential advantages and challenges of integrating AI with XML development tools and languages, the presentation seeks to inspire thoughtful conversation around the future of XML development. We’ll not only delve into the technical aspects of AI-powered XML development but also discuss practical implications and possible future directions.
Webinar: Designing a schema for a Data Warehouse
Are you new to data warehouses (DWH)? Do you need to check whether your data warehouse follows the best practices for a good design? In both cases, this webinar is for you.
A data warehouse is a central relational database that contains all measurements about a business or an organisation. This data comes from a variety of heterogeneous data sources, which includes databases of any type that back the applications used by the company, data files exported by some applications, or APIs provided by internal or external services.
But designing a data warehouse correctly is a hard task, which requires gathering information about the business processes that need to be analysed in the first place. These processes must be translated into so-called star schemas, which means, denormalised databases where each table represents a dimension or facts.
We will discuss these topics:
- How to gather information about a business;
- Understanding dictionaries and how to identify business entities;
- Dimensions and facts;
- Setting a table granularity;
- Types of facts;
- Types of dimensions;
- Snowflakes and how to avoid them;
- Expanding existing dimensions and facts.
Generating privacy-protected synthetic data using Secludy and Milvus
During this demo, the founders of Secludy will demonstrate how their system utilizes Milvus to store and manipulate embeddings for generating privacy-protected synthetic data. Their approach not only maintains the confidentiality of the original data but also enhances the utility and scalability of LLMs under privacy constraints. Attendees, including machine learning engineers, data scientists, and data managers, will witness first-hand how Secludy's integration with Milvus empowers organizations to harness the power of LLMs securely and efficiently.
Building Production Ready Search Pipelines with Spark and Milvus
Spark is the widely used ETL tool for processing, indexing and ingesting data to serving stack for search. Milvus is the production-ready open-source vector database. In this talk we will show how to use Spark to process unstructured data to extract vector representations, and push the vectors to Milvus vector database for search serving.
Salesforce Integration for Bonterra Impact Management (fka Social Solutions A...
Sidekick Solutions uses Bonterra Impact Management (fka Social Solutions Apricot) and automation solutions to integrate data for business workflows.
We believe integration and automation are essential to user experience and the promise of efficient work through technology. Automation is the critical ingredient to realizing that full vision. We develop integration products and services for Bonterra Case Management software to support the deployment of automations for a variety of use cases.
This video focuses on integration of Salesforce with Bonterra Impact Management.
Interested in deploying an integration with Salesforce for Bonterra Impact Management? Contact us at sales@sidekicksolutionsllc.com to discuss next steps.
How to Interpret Trends in the Kalyan Rajdhani Mix Chart.pdf
A Mix Chart displays historical data of numbers in a graphical or tabular form. The Kalyan Rajdhani Mix Chart specifically shows the results of a sequence of numbers over different periods.
Skybuffer SAM4U tool for SAP license adoption
Manage and optimize your license adoption and consumption with SAM4U, an SAP free customer software asset management tool.
SAM4U, an SAP complimentary software asset management tool for customers, delivers a detailed and well-structured overview of license inventory and usage with a user-friendly interface. We offer a hosted, cost-effective, and performance-optimized SAM4U setup in the Skybuffer Cloud environment. You retain ownership of the system and data, while we manage the ABAP 7.58 infrastructure, ensuring fixed Total Cost of Ownership (TCO) and exceptional services through the SAP Fiori interface.
みなさんこんにちはこれ何文字まで入るの？40文字以下不可とか本当に意味わからないけどこれ限界文字数書いてないからマジでやばい文字数いけるんじゃないの？えこ...
ここ3000字までしか入らないけどタイトルの方がたくさん文字入ると思います。
Unlock the Future of Search with MongoDB Atlas_ Vector Search Unleashed.pdf
Discover how MongoDB Atlas and vector search technology can revolutionize your application's search capabilities. This comprehensive presentation covers:
* What is Vector Search?
* Importance and benefits of vector search
* Practical use cases across various industries
* Step-by-step implementation guide
* Live demos with code snippets
* Enhancing LLM capabilities with vector search
* Best practices and optimization strategies
Perfect for developers, AI enthusiasts, and tech leaders. Learn how to leverage MongoDB Atlas to deliver highly relevant, context-aware search results, transforming your data retrieval process. Stay ahead in tech innovation and maximize the potential of your applications.
#MongoDB #VectorSearch #AI #SemanticSearch #TechInnovation #DataScience #LLM #MachineLearning #SearchTechnology
GraphRAG for Life Science to increase LLM accuracy
GraphRAG for life science domain, where you retriever information from biomedical knowledge graphs using LLMs to increase the accuracy and performance of generated answers
Driving Business Innovation: Latest Generative AI Advancements & Success Story
Are you ready to revolutionize how you handle data? Join us for a webinar where we’ll bring you up to speed with the latest advancements in Generative AI technology and discover how leveraging FME with tools from giants like Google Gemini, Amazon, and Microsoft OpenAI can supercharge your workflow efficiency.
During the hour, we’ll take you through:
Guest Speaker Segment with Hannah Barrington: Dive into the world of dynamic real estate marketing with Hannah, the Marketing Manager at Workspace Group. Hear firsthand how their team generates engaging descriptions for thousands of office units by integrating diverse data sources—from PDF floorplans to web pages—using FME transformers, like OpenAIVisionConnector and AnthropicVisionConnector. This use case will show you how GenAI can streamline content creation for marketing across the board.
Ollama Use Case: Learn how Scenario Specialist Dmitri Bagh has utilized Ollama within FME to input data, create custom models, and enhance security protocols. This segment will include demos to illustrate the full capabilities of FME in AI-driven processes.
Custom AI Models: Discover how to leverage FME to build personalized AI models using your data. Whether it’s populating a model with local data for added security or integrating public AI tools, find out how FME facilitates a versatile and secure approach to AI.
We’ll wrap up with a live Q&A session where you can engage with our experts on your specific use cases, and learn more about optimizing your data workflows with AI.
This webinar is ideal for professionals seeking to harness the power of AI within their data management systems while ensuring high levels of customization and security. Whether you're a novice or an expert, gain actionable insights and strategies to elevate your data processes. Join us to see how FME and AI can revolutionize how you work with data!
Your One-Stop Shop for Python Success: Top 10 US Python Development Providers
Simplify your search for a reliable Python development partner! This list presents the top 10 trusted US providers offering comprehensive Python development services, ensuring your project's success from conception to completion.
Digital Marketing Trends in 2024 | Guide for Staying Ahead
https://www.wask.co/ebooks/digital-marketing-trends-in-2024
Feeling lost in the digital marketing whirlwind of 2024? Technology is changing, consumer habits are evolving, and staying ahead of the curve feels like a never-ending pursuit. This e-book is your compass. Dive into actionable insights to handle the complexities of modern marketing. From hyper-personalization to the power of user-generated content, learn how to build long-term relationships with your audience and unlock the secrets to success in the ever-shifting digital landscape.
TrustArc Webinar - 2024 Global Privacy Survey
How does your privacy program stack up against your peers? What challenges are privacy teams tackling and prioritizing in 2024?
In the fifth annual Global Privacy Benchmarks Survey, we asked over 1,800 global privacy professionals and business executives to share their perspectives on the current state of privacy inside and outside of their organizations. This year’s report focused on emerging areas of importance for privacy and compliance professionals, including considerations and implications of Artificial Intelligence (AI) technologies, building brand trust, and different approaches for achieving higher privacy competence scores.
See how organizational priorities and strategic approaches to data security and privacy are evolving around the globe.
This webinar will review:
- The top 10 privacy insights from the fifth annual Global Privacy Benchmarks Survey
- The top challenges for privacy leaders, practitioners, and organizations in 2024
- Key themes to consider in developing and maintaining your privacy program
June Patch Tuesday
Ivanti’s Patch Tuesday breakdown goes beyond patching your applications and brings you the intelligence and guidance needed to prioritize where to focus your attention first. Catch early analysis on our Ivanti blog, then join industry expert Chris Goettl for the Patch Tuesday Webinar Event. There we’ll do a deep dive into each of the bulletins and give guidance on the risks associated with the newly-identified vulnerabilities.
Energy Efficient Video Encoding for Cloud and Edge Computing Instances
Energy Efficient Video Encoding for Cloud and Edge Computing Instances
Ocean lotus Threat actors project by John Sitima 2024 (1).pptx
Ocean Lotus cyber threat actors represent a sophisticated, persistent, and politically motivated group that poses a significant risk to organizations and individuals in the Southeast Asian region. Their continuous evolution and adaptability underscore the need for robust cybersecurity measures and international cooperation to identify and mitigate the threats posed by such advanced persistent threat groups.
National Security Agency - NSA mobile device best practices
Threats to mobile devices are more prevalent and increasing in scope and complexity. Users of mobile devices desire to take full advantage of the features
available on those devices, but many of the features provide convenience and capability but sacrifice security. This best practices guide outlines steps the users can take to better protect personal devices and information.
Project Management Semester Long Project - Acuity
Acuity is an innovative learning app designed to transform the way you engage with knowledge. Powered by AI technology, Acuity takes complex topics and distills them into concise, interactive summaries that are easy to read & understand. Whether you're exploring the depths of quantum mechanics or seeking insight into historical events, Acuity provides the key information you need without the burden of lengthy texts.
Recently uploaded (20)
Generating privacy-protected synthetic data using Secludy and Milvus
Generating privacy-protected synthetic data using Secludy and Milvus
Building Production Ready Search Pipelines with Spark and Milvus
Building Production Ready Search Pipelines with Spark and Milvus
Salesforce Integration for Bonterra Impact Management (fka Social Solutions A...
Salesforce Integration for Bonterra Impact Management (fka Social Solutions A...
How to Interpret Trends in the Kalyan Rajdhani Mix Chart.pdf
How to Interpret Trends in the Kalyan Rajdhani Mix Chart.pdf
みなさんこんにちはこれ何文字まで入るの？40文字以下不可とか本当に意味わからないけどこれ限界文字数書いてないからマジでやばい文字数いけるんじゃないの？えこ...
みなさんこんにちはこれ何文字まで入るの？40文字以下不可とか本当に意味わからないけどこれ限界文字数書いてないからマジでやばい文字数いけるんじゃないの？えこ...
Unlock the Future of Search with MongoDB Atlas_ Vector Search Unleashed.pdf
Unlock the Future of Search with MongoDB Atlas_ Vector Search Unleashed.pdf
GraphRAG for Life Science to increase LLM accuracy
GraphRAG for Life Science to increase LLM accuracy
Driving Business Innovation: Latest Generative AI Advancements & Success Story
Driving Business Innovation: Latest Generative AI Advancements & Success Story
Your One-Stop Shop for Python Success: Top 10 US Python Development Providers
Your One-Stop Shop for Python Success: Top 10 US Python Development Providers
Digital Marketing Trends in 2024 | Guide for Staying Ahead
Digital Marketing Trends in 2024 | Guide for Staying Ahead
Energy Efficient Video Encoding for Cloud and Edge Computing Instances
Energy Efficient Video Encoding for Cloud and Edge Computing Instances
Ocean lotus Threat actors project by John Sitima 2024 (1).pptx
Ocean lotus Threat actors project by John Sitima 2024 (1).pptx
National Security Agency - NSA mobile device best practices
National Security Agency - NSA mobile device best practices
Prokaryotic Gene Expression Asignment
- 1. P3/M3/D3 Prokaryotic Gene Expression Unit 18: Genetics and Genetic Engineering Assessed by Teresa Greer Start date: February 23rd 2009 Hand in date: March 16th 2009 Purpose Learners will be examining the issues surrounding genetics and genetic engineering with specific focus on the use of prokaryotes for this purpose. Issues the learners will be exam- ining will include the types of cells used for genetic modification, the benefits of genetic modification and the uses. Scenario You are working for a school education program and the brief you have been given is to provide local schools with relevant information about the benefits of genetic engineering, the methods used to genetically modify organisms and what organisms are used. Task A This provides evidence for P3 Part one: You need to identify a current use for genetic engineering in industry and prepare a five minute presentation discussing the techniques and applications of this area of sci- ence. Part two: You will be investigating the control of prokaryotic gene expression looking spe- cifically at Beta Galactosidase. On completion you will write a full practical report with discussion. Hand in date March 5th Task B This provides evidence for M3 For this task you will be examining how gene expression is controlled in the Lac Operon. For this you need to produce an A3 poster that shows the lac operon and discusses control mechanisms. Hand in date March 9th Task C This provides evidence for D3 This provides evidence for key skills In genetic engineering prokaryotic cells are preferred over eukaryotic cells? Why do you think this is? Write a 1,000 word essay with the title Compare and Contrast the use of Prokaryotic and Eukaryotic Cells in Genetic Engineering You must use at least five references and at least one of these must be non-internet based hand in date March 16th BTEC National Diploma Applied Science Prokaryotic Gene Expression
- 2. Prokaryotic Gene Expression To achieve a pass grade To achieve a merit To achieve a distinction the evidence must grade the evidence grade the evidence show that the learner is must show that the must show that the able to: learner is able to: learner is able to: Conduct and Report on Explain how expression Explain the use of pro- a practical investigation of a prokaryotic gene is karyotes in preference into the control of ex- controlled to eukaryotes in gene pression of a prokary- technology otic gene Assessed by: Teresa Greer Internally verified by: Hand in date: March 16th 2009 BTEC National Diploma Applied Science Prokaryotic Gene Expression