The capability to anticipate ovulation well in advance, and to then detect ovulation independently of the predictive signals, is unique to the bioZhena technology. This unique capability results from the mode of action, further discussed in the Alphabet of bioZhena under Modus operandi (MO). http://biozhena.wordpress.com/2007/11/28/the-alphabet-of-biozhena
See also under Mysterious conceptions - or the non-existence thereof. From the MO also follows the broad applicability of the technology, which is another feature that distinguishes the Ovulona from any other product addressing fertility status and ovulation monitoring. For a potential impact of the technology on public health, see under Sexually transmitted diseases, and also under Cervical cancer and under Smoking.
It could be argued that the greatest aspect of the bioZhena project is the idea of introducing – via the affordable personal fertility monitoring method – a general, routinely usable, women’s health tracking and diagnostic tool, with the potential to impact on several important areas of public health.
IntOGen, Integrative Oncogenomics for Personal Cancer Genomeschristian.perez
IntOGen was presented September, 11th at the CSHL Meeting on Personal Genomes. The talk was given by Christian Perez-Llamas and he presented the main features of the current version and the advances of IntOGen 2.0 to store, analyze and visualize next generation sequencing data from cancer samples.
CSHL Meeting on Personal Cancer Genomes web: http://meetings.cshl.edu/meetings/person10.shtml
The document describes a Laboratory Assistant System (LAS) that was created through a collaboration between IRCC and Politecnico di Torino starting in May 2011. The LAS aims to provide structured management of samples, biological data, and molecular data from instruments and public databases. It allows for complex queries across multiple databases and integrative analysis with annotation tools. The LAS has three phases of development, with the initial phase focusing on biobanking and xenopatients management. It currently manages thousands of samples and mice. Future phases will integrate additional facilities and experimental data types.
The document discusses various applications and techniques of DNA microarrays, including summarizing key points about Affymetrix GeneChips, spotted microarrays, experimental design, data analysis, and several case studies on various topics like ovarian cancer, Sjogren's syndrome, wine yeast genomics, and norovirus genotyping. Microarrays allow analysis of gene expression patterns and copy number variations across genomes through comparative hybridization experiments. The document provides an overview of microarray technology and applications in genomic and biomedical research.
The document discusses the Myriad case regarding whether gene sequences and diagnostic methods can be patented. It summarizes the case history from the District Court of Southern District of New York to the US Supreme Court. It also analyzes the key issues around whether isolated DNA, diagnostic methods, and transformative steps in a process are eligible for patent protection under US law. The highest court reconsidered the Prometheus case and determined that well-understood routine steps or merely comparing information would not be patentable.
Fruit breedomics workshop wp6 a cost effective strategy for mas riccardo velascofruitbreedomics
This document summarizes the marker assisted selection (MAS) strategy used in the apple breeding program at Fondazione Edmund Mach. The program uses molecular markers to efficiently select for resistance traits and quality traits. Over 10,000 apple genotypes have been evaluated using MAS to pyramid resistance genes from different sources into new cultivars. The breeding program maintains a large germplasm collection and performs extensive crosses between sources of resistance. Offspring are rapidly screened using optimized DNA extraction and marker analysis pipelines, allowing over 10,000 plants to be analyzed in just 3 weeks. MAS is accelerating the development of new apple varieties with improved combinations of resistance to important diseases like apple scab and powdery mildew.
This document summarizes a presentation on using micro and nanotechnologies for cancer diagnostics and therapy. It discusses using various technologies like microarrays, comparative genomic hybridization, and integration of genome and transcriptome data to analyze cancer at multiple levels. In particular, it focuses on using these techniques to study neuroblastoma and identify genetic signatures that can predict patient outcomes and survival. Signatures identified include miRNA profiles and their interactions with mRNA that are associated with poor survival in neuroblastoma patients.
1) Noviogendix is a spin-off company from Radboud University Nijmegen Medical Center that focuses on molecular diagnostics, specifically in oncology and infectious diseases.
2) Its core business is operating a specialty oncology laboratory offering prostate cancer and other genitourinary disease tests using technologies like real-time PCR.
3) The company also conducts research to develop new biomarkers and molecular profiles of cancers using its biorepositories, with a focus on intellectual property licensing opportunities.
This document summarizes techniques for rapid diagnostic testing by copying nature. It discusses how current culture-based detection of bacteria from clinical samples can take 2-3 days. New molecular detection methods directly detect bacteria in 1-2 hours but sample preparation is the limiting step. The document presents new sample preparation techniques inspired by nature, such as magnetic particle-based extraction of DNA from bacteria and a pelleting method to separate bacteria from blood similar to how horseshoe crabs capture bacteria. Faster sample preparation methods could enable more rapid identification of bacteria using downstream techniques like PCR, sequencing, or mass spectrometry.
IntOGen, Integrative Oncogenomics for Personal Cancer Genomeschristian.perez
IntOGen was presented September, 11th at the CSHL Meeting on Personal Genomes. The talk was given by Christian Perez-Llamas and he presented the main features of the current version and the advances of IntOGen 2.0 to store, analyze and visualize next generation sequencing data from cancer samples.
CSHL Meeting on Personal Cancer Genomes web: http://meetings.cshl.edu/meetings/person10.shtml
The document describes a Laboratory Assistant System (LAS) that was created through a collaboration between IRCC and Politecnico di Torino starting in May 2011. The LAS aims to provide structured management of samples, biological data, and molecular data from instruments and public databases. It allows for complex queries across multiple databases and integrative analysis with annotation tools. The LAS has three phases of development, with the initial phase focusing on biobanking and xenopatients management. It currently manages thousands of samples and mice. Future phases will integrate additional facilities and experimental data types.
The document discusses various applications and techniques of DNA microarrays, including summarizing key points about Affymetrix GeneChips, spotted microarrays, experimental design, data analysis, and several case studies on various topics like ovarian cancer, Sjogren's syndrome, wine yeast genomics, and norovirus genotyping. Microarrays allow analysis of gene expression patterns and copy number variations across genomes through comparative hybridization experiments. The document provides an overview of microarray technology and applications in genomic and biomedical research.
The document discusses the Myriad case regarding whether gene sequences and diagnostic methods can be patented. It summarizes the case history from the District Court of Southern District of New York to the US Supreme Court. It also analyzes the key issues around whether isolated DNA, diagnostic methods, and transformative steps in a process are eligible for patent protection under US law. The highest court reconsidered the Prometheus case and determined that well-understood routine steps or merely comparing information would not be patentable.
Fruit breedomics workshop wp6 a cost effective strategy for mas riccardo velascofruitbreedomics
This document summarizes the marker assisted selection (MAS) strategy used in the apple breeding program at Fondazione Edmund Mach. The program uses molecular markers to efficiently select for resistance traits and quality traits. Over 10,000 apple genotypes have been evaluated using MAS to pyramid resistance genes from different sources into new cultivars. The breeding program maintains a large germplasm collection and performs extensive crosses between sources of resistance. Offspring are rapidly screened using optimized DNA extraction and marker analysis pipelines, allowing over 10,000 plants to be analyzed in just 3 weeks. MAS is accelerating the development of new apple varieties with improved combinations of resistance to important diseases like apple scab and powdery mildew.
This document summarizes a presentation on using micro and nanotechnologies for cancer diagnostics and therapy. It discusses using various technologies like microarrays, comparative genomic hybridization, and integration of genome and transcriptome data to analyze cancer at multiple levels. In particular, it focuses on using these techniques to study neuroblastoma and identify genetic signatures that can predict patient outcomes and survival. Signatures identified include miRNA profiles and their interactions with mRNA that are associated with poor survival in neuroblastoma patients.
1) Noviogendix is a spin-off company from Radboud University Nijmegen Medical Center that focuses on molecular diagnostics, specifically in oncology and infectious diseases.
2) Its core business is operating a specialty oncology laboratory offering prostate cancer and other genitourinary disease tests using technologies like real-time PCR.
3) The company also conducts research to develop new biomarkers and molecular profiles of cancers using its biorepositories, with a focus on intellectual property licensing opportunities.
This document summarizes techniques for rapid diagnostic testing by copying nature. It discusses how current culture-based detection of bacteria from clinical samples can take 2-3 days. New molecular detection methods directly detect bacteria in 1-2 hours but sample preparation is the limiting step. The document presents new sample preparation techniques inspired by nature, such as magnetic particle-based extraction of DNA from bacteria and a pelleting method to separate bacteria from blood similar to how horseshoe crabs capture bacteria. Faster sample preparation methods could enable more rapid identification of bacteria using downstream techniques like PCR, sequencing, or mass spectrometry.
This document contains summaries of several laboratory experiences and seminars:
1. The first discusses reviewing pipetting techniques, including large and small volume exercises with a focus on micro-pipettes. Precise pipetting is important for accurate experiment results.
2. The second describes a laboratory reviewing microscopy and techniques, including using different microscope parts and learning micro-techniques like fluorescence microscopy.
3. The third summarizes a three-day workshop on techniques like DNA extraction, PCR, agarose gel electrophoresis, and SDS-PAGE to illustrate the central dogma and protein analysis. These are common tools in genetics and biology.
4. Further summaries discuss seminars on topics including nanoparticles, electron
24x7 Automated Behavior Tracking For Rodent Safety Pharmacology & PhenotypingInsideScientific
Actual Analytics Ltd and its development partners present an exclusive webinar describing the applications of a novel Home Cage Analysis system for tracking behavior in group housed rodents, with retained identity, in regular IVC racked home cages.
In this webinar, Dr. Will Redfern of AstraZeneca and Dr. Sara Wells of MRC Harwell discuss 24/7 monitoring of group-housed rodents in their true IVC racked home cage environment for safety pharmacology and phenotyping applications.
Discussions describe the types of new insights that can be obtained from 24/7 monitoring of research animals including activity differences in single and group animals and body temperature profiles in response to drug treatment. Presenters show how they are using this system in various applications from safety pharmacology in rats through to phenotyping studies in mice.
4 Factors That Affect Research ReproducibilityCellero
Learn how to improve reproducibility in your lab by focusing on these key sources of variability. Insights, data, and tips from an immunology and inflammation research laboratory. https://astartebio.com/research/
1. This laboratory focused on practicing proper micropipetting techniques. Students mixed solutions of water and dyes to practice using micropipettes accurately and safely. This helped prepare them for upcoming labs requiring precise measurements.
2. Students learned different microscopy techniques, including fluorescence and bright field microscopy. They took photomicrographs using a camera attached to the microscope.
3. Over three days, students extracted their own DNA from mouth cells, ran PCR tests to determine if a patient sample had diabetes, and used gel electrophoresis to analyze protein biomarkers and diagnose a lysosomal storage disorder.
Pipetting techniques and concepts were reviewed, including proper use of micro-pipettes, as precision is important to avoid experimental errors. Microscopy and techniques like fluorescence microscopy and phase-contrast microscopy were also covered. Students learned how to use different microscope parts and take photomicrographs. A three-day workshop taught DNA extraction, PCR, agarose gel electrophoresis, SDS-PAGE, and other genomics and proteomics techniques. These laboratory skills are important tools in biology and genetics to analyze genes and proteins.
A set of 15 bioZhena Corp. slides highlights what matters the most at this stage (not a formal presentation). Best to view it in the Slide Show mode. The Speaker Notes contain some additional information.
Methods For Improving The Cellular Uptake Of Dna Origami...Christina Santos
The presentation will focus on how the novel "Fifteen Dogs" has been taken up and promoted via Twitter. It will use Anne Freadman's concept of "uptake" to analyze how different Twitter users discuss and spread information about the novel on the platform, effectively promoting the work. The presentation will examine how Twitter serves as a means for novels to be "taken up" and transformed into promotional devices.
This document summarizes three laboratory experiences:
1. A microscopy lab taught techniques like bright field, dark field, and phase contrast microscopy as well as photomicrography.
2. A basic microbiology lab covered aseptic techniques, bacterial culture, and bacterial shape identification using micropipettes.
3. A three-day workshop on cancer biology covered DNA extraction, electrophoresis, oncogenes, protein synthesis and detection.
Residual Protein Detection System for Surgical Instruments Thomas Overbey
The document introduces the ProReveal fluorescence detection system, which can detect trace amounts of residual proteins on surgical instruments after cleaning. It describes the system's development in the UK and how it works with an easy 3-minute test. The system provides numerical and visual results, stores test data, and can be used to monitor cleaning processes and equipment over time through trend analysis. It is presented as a new tool that can help further the goal of ensuring surgical instruments are absolutely clean after reprocessing.
This document summarizes four case studies of issues encountered during bioanalytical method validation:
1) An analyst constructed standard curves inconsistently between assay runs, undermining the reliability of concentration values.
2) An assay was used for many years without re-validation despite changes in equipment affecting analyte retention times.
3) Some subjects showed non-physiological "U-shaped" concentration-time curves that were accepted without investigation.
4) Hundreds of samples were rendered unusable due to an improperly specified and sub-potent reagent, costing $1 million to resolve.
The document cautions against overreliance on automation and lack of primary data examination, and emphasizes
The document discusses various technologies used at the House Ear Institute including genomics, proteomics, and imaging. It describes how researchers are using these tools to study diseases like neurofibromatosis type 2 (NF2) at the molecular level in order to develop personalized treatments and therapies. Maintaining high quality biospecimens is important for enabling various types of research.
The laboratory plays several key roles in outbreak management:
1) It confirms outbreaks by identifying the causative agent through tests like gram staining, latex agglutination, culture and sensitivity, and PCR.
2) It investigates outbreaks by identifying transmission modes and high-risk groups to help design effective control interventions.
3) It monitors endemic disease trends, confirms diagnoses using laboratory criteria, and tracks resistance patterns and pathogen subtypes. The laboratory thus supports prevention, early detection, investigation, and post-intervention control of disease outbreaks.
The document discusses various advances in medical technology in the 21st century, including 4D ultrasound that provides more detailed moving images of fetuses; microfluidic chips that can quickly diagnose the flu; non-invasive methods for detecting diabetes; brain-computer interfaces; ingestible camera pills; smart capsules equipped with tools and sensors; engineered bacteria that prevent tooth decay; artificial lymph nodes; sensors for monitoring asthma and detecting oral cancer from saliva; biological pacemakers; prosthetics that provide feedback to the user; smart contact lenses that monitor eye pressure; speech restoration devices; and absorbable heart stents. The technologies presented help with diagnoses, treatment, and quality of life.
The document describes experiments conducted to identify an unknown microorganism. Samples from two patients, labeled Culture A and Culture B, were tested using a Gram stain. Culture A showed pink rod-shaped bacteria, while Culture B showed round purplish-pink bacteria. An antibiotic test found that chloramphenicol and tetracycline were most effective at inhibiting the growth of both bacterial cultures. Based on the morphological and antibiotic test results, the unknown microorganism was identified as Citrobacter Freundii.
This document provides guidelines for the derivation and characterization of cell substrates used in the production of biotechnological and biological products. It recommends documenting the source, history and generation of the cell substrate, including any genetic manipulation. Cell banking procedures should prevent contamination and ensure each container can be traced. Characterization of cell banks includes testing for identity, purity, stability and screening for adventitious agents. The goal is to help ensure the quality, safety and consistency of products derived from cell substrates.
This document discusses innovations and breakthroughs in in vitro fertilization (IVF). It covers the following topics in 3 sentences or less:
Genetic screening techniques like preimplantation genetic diagnosis (PGD) and preimplantation genetic screening (PGS) are discussed to select embryos without genetic disorders or the highest chance of implantation. Time-lapse monitoring is presented as a way to continuously monitor embryo development in real-time without disruptions. Stem cell therapy and its potential role in inducing ovarian regeneration and sustained ovarian function is briefly covered.
The W3C PROV standard:data model for the provenance of information, and enab...Paolo Missier
The document summarizes the W3C PROV standard for representing provenance data. It describes provenance as recording the origins and processes involved in producing data. This allows researchers to verify results and understand how data was transformed. The PROV standard defines a model for provenance using entities, activities, and agents. It represents their relationships through properties like wasGeneratedBy and used. This enables tracking data derivations and transformations to ensure trust in published results.
The document provides an overview of a company called Miroculus that has developed an accurate, easy to use, and affordable microRNA detection platform. Some key points:
1. Miroculus has 4 full-time employees and has labs in Heidelberg and an office in Mexico City. They are developing a platform to detect circulating microRNAs which can be diagnostic biomarkers for diseases like cancer.
2. Their platform includes an accurate bioassay that can detect microRNAs from plasma samples, a low-cost device to run the bioassay, and data analytics algorithms. This allows quantitative and qualitative molecular monitoring for disease in a simple and affordable way.
3. They have prototypes of the bioassay,
This document discusses how new sensor and intelligent device technologies have the potential to transform healthcare delivery by bringing care closer to patients' homes. It describes how these non-intrusive technologies could enable patients to recover more quickly after surgery and benefit from virtual reality applications. The document also examines how wearable devices and sensors that can monitor vital signs could support more integrated, patient-centered care models by reducing dependence on providers and facilitating remote care, monitoring and early detection of health issues. Machine learning and big data analytics are discussed as ways to generate insights from the large and growing volumes of patient data that these new digital devices and sensors are capturing.
Does Over-Masturbation Contribute to Chronic Prostatitis.pptxwalterHu5
In some case, your chronic prostatitis may be related to over-masturbation. Generally, natural medicine Diuretic and Anti-inflammatory Pill can help mee get a cure.
This document contains summaries of several laboratory experiences and seminars:
1. The first discusses reviewing pipetting techniques, including large and small volume exercises with a focus on micro-pipettes. Precise pipetting is important for accurate experiment results.
2. The second describes a laboratory reviewing microscopy and techniques, including using different microscope parts and learning micro-techniques like fluorescence microscopy.
3. The third summarizes a three-day workshop on techniques like DNA extraction, PCR, agarose gel electrophoresis, and SDS-PAGE to illustrate the central dogma and protein analysis. These are common tools in genetics and biology.
4. Further summaries discuss seminars on topics including nanoparticles, electron
24x7 Automated Behavior Tracking For Rodent Safety Pharmacology & PhenotypingInsideScientific
Actual Analytics Ltd and its development partners present an exclusive webinar describing the applications of a novel Home Cage Analysis system for tracking behavior in group housed rodents, with retained identity, in regular IVC racked home cages.
In this webinar, Dr. Will Redfern of AstraZeneca and Dr. Sara Wells of MRC Harwell discuss 24/7 monitoring of group-housed rodents in their true IVC racked home cage environment for safety pharmacology and phenotyping applications.
Discussions describe the types of new insights that can be obtained from 24/7 monitoring of research animals including activity differences in single and group animals and body temperature profiles in response to drug treatment. Presenters show how they are using this system in various applications from safety pharmacology in rats through to phenotyping studies in mice.
4 Factors That Affect Research ReproducibilityCellero
Learn how to improve reproducibility in your lab by focusing on these key sources of variability. Insights, data, and tips from an immunology and inflammation research laboratory. https://astartebio.com/research/
1. This laboratory focused on practicing proper micropipetting techniques. Students mixed solutions of water and dyes to practice using micropipettes accurately and safely. This helped prepare them for upcoming labs requiring precise measurements.
2. Students learned different microscopy techniques, including fluorescence and bright field microscopy. They took photomicrographs using a camera attached to the microscope.
3. Over three days, students extracted their own DNA from mouth cells, ran PCR tests to determine if a patient sample had diabetes, and used gel electrophoresis to analyze protein biomarkers and diagnose a lysosomal storage disorder.
Pipetting techniques and concepts were reviewed, including proper use of micro-pipettes, as precision is important to avoid experimental errors. Microscopy and techniques like fluorescence microscopy and phase-contrast microscopy were also covered. Students learned how to use different microscope parts and take photomicrographs. A three-day workshop taught DNA extraction, PCR, agarose gel electrophoresis, SDS-PAGE, and other genomics and proteomics techniques. These laboratory skills are important tools in biology and genetics to analyze genes and proteins.
A set of 15 bioZhena Corp. slides highlights what matters the most at this stage (not a formal presentation). Best to view it in the Slide Show mode. The Speaker Notes contain some additional information.
Methods For Improving The Cellular Uptake Of Dna Origami...Christina Santos
The presentation will focus on how the novel "Fifteen Dogs" has been taken up and promoted via Twitter. It will use Anne Freadman's concept of "uptake" to analyze how different Twitter users discuss and spread information about the novel on the platform, effectively promoting the work. The presentation will examine how Twitter serves as a means for novels to be "taken up" and transformed into promotional devices.
This document summarizes three laboratory experiences:
1. A microscopy lab taught techniques like bright field, dark field, and phase contrast microscopy as well as photomicrography.
2. A basic microbiology lab covered aseptic techniques, bacterial culture, and bacterial shape identification using micropipettes.
3. A three-day workshop on cancer biology covered DNA extraction, electrophoresis, oncogenes, protein synthesis and detection.
Residual Protein Detection System for Surgical Instruments Thomas Overbey
The document introduces the ProReveal fluorescence detection system, which can detect trace amounts of residual proteins on surgical instruments after cleaning. It describes the system's development in the UK and how it works with an easy 3-minute test. The system provides numerical and visual results, stores test data, and can be used to monitor cleaning processes and equipment over time through trend analysis. It is presented as a new tool that can help further the goal of ensuring surgical instruments are absolutely clean after reprocessing.
This document summarizes four case studies of issues encountered during bioanalytical method validation:
1) An analyst constructed standard curves inconsistently between assay runs, undermining the reliability of concentration values.
2) An assay was used for many years without re-validation despite changes in equipment affecting analyte retention times.
3) Some subjects showed non-physiological "U-shaped" concentration-time curves that were accepted without investigation.
4) Hundreds of samples were rendered unusable due to an improperly specified and sub-potent reagent, costing $1 million to resolve.
The document cautions against overreliance on automation and lack of primary data examination, and emphasizes
The document discusses various technologies used at the House Ear Institute including genomics, proteomics, and imaging. It describes how researchers are using these tools to study diseases like neurofibromatosis type 2 (NF2) at the molecular level in order to develop personalized treatments and therapies. Maintaining high quality biospecimens is important for enabling various types of research.
The laboratory plays several key roles in outbreak management:
1) It confirms outbreaks by identifying the causative agent through tests like gram staining, latex agglutination, culture and sensitivity, and PCR.
2) It investigates outbreaks by identifying transmission modes and high-risk groups to help design effective control interventions.
3) It monitors endemic disease trends, confirms diagnoses using laboratory criteria, and tracks resistance patterns and pathogen subtypes. The laboratory thus supports prevention, early detection, investigation, and post-intervention control of disease outbreaks.
The document discusses various advances in medical technology in the 21st century, including 4D ultrasound that provides more detailed moving images of fetuses; microfluidic chips that can quickly diagnose the flu; non-invasive methods for detecting diabetes; brain-computer interfaces; ingestible camera pills; smart capsules equipped with tools and sensors; engineered bacteria that prevent tooth decay; artificial lymph nodes; sensors for monitoring asthma and detecting oral cancer from saliva; biological pacemakers; prosthetics that provide feedback to the user; smart contact lenses that monitor eye pressure; speech restoration devices; and absorbable heart stents. The technologies presented help with diagnoses, treatment, and quality of life.
The document describes experiments conducted to identify an unknown microorganism. Samples from two patients, labeled Culture A and Culture B, were tested using a Gram stain. Culture A showed pink rod-shaped bacteria, while Culture B showed round purplish-pink bacteria. An antibiotic test found that chloramphenicol and tetracycline were most effective at inhibiting the growth of both bacterial cultures. Based on the morphological and antibiotic test results, the unknown microorganism was identified as Citrobacter Freundii.
This document provides guidelines for the derivation and characterization of cell substrates used in the production of biotechnological and biological products. It recommends documenting the source, history and generation of the cell substrate, including any genetic manipulation. Cell banking procedures should prevent contamination and ensure each container can be traced. Characterization of cell banks includes testing for identity, purity, stability and screening for adventitious agents. The goal is to help ensure the quality, safety and consistency of products derived from cell substrates.
This document discusses innovations and breakthroughs in in vitro fertilization (IVF). It covers the following topics in 3 sentences or less:
Genetic screening techniques like preimplantation genetic diagnosis (PGD) and preimplantation genetic screening (PGS) are discussed to select embryos without genetic disorders or the highest chance of implantation. Time-lapse monitoring is presented as a way to continuously monitor embryo development in real-time without disruptions. Stem cell therapy and its potential role in inducing ovarian regeneration and sustained ovarian function is briefly covered.
The W3C PROV standard:data model for the provenance of information, and enab...Paolo Missier
The document summarizes the W3C PROV standard for representing provenance data. It describes provenance as recording the origins and processes involved in producing data. This allows researchers to verify results and understand how data was transformed. The PROV standard defines a model for provenance using entities, activities, and agents. It represents their relationships through properties like wasGeneratedBy and used. This enables tracking data derivations and transformations to ensure trust in published results.
The document provides an overview of a company called Miroculus that has developed an accurate, easy to use, and affordable microRNA detection platform. Some key points:
1. Miroculus has 4 full-time employees and has labs in Heidelberg and an office in Mexico City. They are developing a platform to detect circulating microRNAs which can be diagnostic biomarkers for diseases like cancer.
2. Their platform includes an accurate bioassay that can detect microRNAs from plasma samples, a low-cost device to run the bioassay, and data analytics algorithms. This allows quantitative and qualitative molecular monitoring for disease in a simple and affordable way.
3. They have prototypes of the bioassay,
This document discusses how new sensor and intelligent device technologies have the potential to transform healthcare delivery by bringing care closer to patients' homes. It describes how these non-intrusive technologies could enable patients to recover more quickly after surgery and benefit from virtual reality applications. The document also examines how wearable devices and sensors that can monitor vital signs could support more integrated, patient-centered care models by reducing dependence on providers and facilitating remote care, monitoring and early detection of health issues. Machine learning and big data analytics are discussed as ways to generate insights from the large and growing volumes of patient data that these new digital devices and sensors are capturing.
Does Over-Masturbation Contribute to Chronic Prostatitis.pptxwalterHu5
In some case, your chronic prostatitis may be related to over-masturbation. Generally, natural medicine Diuretic and Anti-inflammatory Pill can help mee get a cure.
Local Advanced Lung Cancer: Artificial Intelligence, Synergetics, Complex Sys...Oleg Kshivets
Overall life span (LS) was 1671.7±1721.6 days and cumulative 5YS reached 62.4%, 10 years – 50.4%, 20 years – 44.6%. 94 LCP lived more than 5 years without cancer (LS=2958.6±1723.6 days), 22 – more than 10 years (LS=5571±1841.8 days). 67 LCP died because of LC (LS=471.9±344 days). AT significantly improved 5YS (68% vs. 53.7%) (P=0.028 by log-rank test). Cox modeling displayed that 5YS of LCP significantly depended on: N0-N12, T3-4, blood cell circuit, cell ratio factors (ratio between cancer cells-CC and blood cells subpopulations), LC cell dynamics, recalcification time, heparin tolerance, prothrombin index, protein, AT, procedure type (P=0.000-0.031). Neural networks, genetic algorithm selection and bootstrap simulation revealed relationships between 5YS and N0-12 (rank=1), thrombocytes/CC (rank=2), segmented neutrophils/CC (3), eosinophils/CC (4), erythrocytes/CC (5), healthy cells/CC (6), lymphocytes/CC (7), stick neutrophils/CC (8), leucocytes/CC (9), monocytes/CC (10). Correct prediction of 5YS was 100% by neural networks computing (error=0.000; area under ROC curve=1.0).
These lecture slides, by Dr Sidra Arshad, offer a quick overview of the physiological basis of a normal electrocardiogram.
Learning objectives:
1. Define an electrocardiogram (ECG) and electrocardiography
2. Describe how dipoles generated by the heart produce the waveforms of the ECG
3. Describe the components of a normal electrocardiogram of a typical bipolar lead (limb II)
4. Differentiate between intervals and segments
5. Enlist some common indications for obtaining an ECG
6. Describe the flow of current around the heart during the cardiac cycle
7. Discuss the placement and polarity of the leads of electrocardiograph
8. Describe the normal electrocardiograms recorded from the limb leads and explain the physiological basis of the different records that are obtained
9. Define mean electrical vector (axis) of the heart and give the normal range
10. Define the mean QRS vector
11. Describe the axes of leads (hexagonal reference system)
12. Comprehend the vectorial analysis of the normal ECG
13. Determine the mean electrical axis of the ventricular QRS and appreciate the mean axis deviation
14. Explain the concepts of current of injury, J point, and their significance
Study Resources:
1. Chapter 11, Guyton and Hall Textbook of Medical Physiology, 14th edition
2. Chapter 9, Human Physiology - From Cells to Systems, Lauralee Sherwood, 9th edition
3. Chapter 29, Ganong’s Review of Medical Physiology, 26th edition
4. Electrocardiogram, StatPearls - https://www.ncbi.nlm.nih.gov/books/NBK549803/
5. ECG in Medical Practice by ABM Abdullah, 4th edition
6. Chapter 3, Cardiology Explained, https://www.ncbi.nlm.nih.gov/books/NBK2214/
7. ECG Basics, http://www.nataliescasebook.com/tag/e-c-g-basics
Muktapishti is a traditional Ayurvedic preparation made from Shoditha Mukta (Purified Pearl), is believed to help regulate thyroid function and reduce symptoms of hyperthyroidism due to its cooling and balancing properties. Clinical evidence on its efficacy remains limited, necessitating further research to validate its therapeutic benefits.
ABDOMINAL TRAUMA in pediatrics part one.drhasanrajab
Abdominal trauma in pediatrics refers to injuries or damage to the abdominal organs in children. It can occur due to various causes such as falls, motor vehicle accidents, sports-related injuries, and physical abuse. Children are more vulnerable to abdominal trauma due to their unique anatomical and physiological characteristics. Signs and symptoms include abdominal pain, tenderness, distension, vomiting, and signs of shock. Diagnosis involves physical examination, imaging studies, and laboratory tests. Management depends on the severity and may involve conservative treatment or surgical intervention. Prevention is crucial in reducing the incidence of abdominal trauma in children.
Rasamanikya is a excellent preparation in the field of Rasashastra, it is used in various Kushtha Roga, Shwasa, Vicharchika, Bhagandara, Vatarakta, and Phiranga Roga. In this article Preparation& Comparative analytical profile for both Formulationon i.e Rasamanikya prepared by Kushmanda swarasa & Churnodhaka Shodita Haratala. The study aims to provide insights into the comparative efficacy and analytical aspects of these formulations for enhanced therapeutic outcomes.
1. Prototypes of Ovulona™
Table of Contents
Overview......................................................................................................... 2
Old Mark 1 prototype ...................................................................................... 3
Old Mark 3 prototype ...................................................................................... 4
Designer’s rendition and a photo of prototype of marketable product.............. 5
Examples of results obtained with both Mark 1 and Mark 3 prototypes ........... 6
Contact: Vaclav Kirsner, Ph.D.
CEO, bioZhena Corporation
83 Davis Ranch Road
P.O. Box 122
Bellvue, Colorado 80512
U.S.A.
E-mail: vaclavkirsner@yahoo.com
Phone: 970-484-1272
1
2. Overview
• Old Mark 1 prototype
Analog version, tested at the First Gynecology Clinic of University of Turin by Dr.
Chiara Benedetto, MD, PhD, after extensive in-house studies.
• Old Mark 3 prototype
Digital version, tested at the Natural Family Planning Clinic of Marquette University
School of Nursing, results published: R.J. Fehring and W.D. Schlaff, J. Nurse-
Midwifery 43(2), 117, 1998. The picture is from the publication.
• Designer’s rendition and a photo of marketable product prototype
Digital microcomputerized version as planned for product launch.
• Examples of results obtained with both Mark 1 and Mark 3 prototypes
a) Example 1 of a baseline cycle from the Turin study using Mark 1 prototype.
b) Three non-baseline cycles from in-house studies using Mark 1 prototype.
c) One of the three cycles shown alone, illustrating features.
d) A non-baseline cycle from the Marquette study using Mark 3 prototype.
e) Example 2 of the baseline cycles from the Turin study using Mark 1 prototype.
f) Example 3 of the LPD aberration in one of the cycles of the Turin baseline subjects.
2
3. Old Mark 1 prototype
Old Mark 1 prototype
Analog version, tested at University of Turin
Old Mark 1 prototype
bioZhena
The first hand-held prototypes were made once we determined which of the numerous
parameters, systematically investigated with our bench-top equipment, were optimal for the
performance of the monitor.
The engineering was performed by a contractor ignorant of the purpose of the device, using
off-the-shelf parts, and having scratched the labeling on the electronic components,
protecting the confidentiality of the technology. That is why the decidedly non-medical
appearance of the Mark 1 prototype, which I carried around inside a custom-made case
similar to a clarinet case.
3
4. Old Mark 3 prototype
Old Mark 3 prototype
Digital version, FDA 510(k), tested at Marquette U.
bioZhena
Photo copied from the publication by Drs. Fehring and Schlaff: Richard J. Fehring and
William D. Schlaff, quot;Accuracy of the Ovulon fertility monitor to predict and detect ovulationquot;,
Journal of Nurse-Midwifery 43 (No. 2), 117 - 120, 1998.The name used then for the clinical-
test prototype was “Ovulon”, but later on we decided the feminine form “Ovulona” is more
fitting for the decidedly feminine (small and beautiful) user-friendly product shown in the next
illustration.
Note: No recharging transformer and no boxy handheld for the marketable product, which
must be elegantly feminine and elegantly user-friendly, if I put it in so many words!
4
5. Designer’s rendition and a photograph
of marketable product prototype
bioZhena
The industrial designer’s rendition of our miniaturized device is on the left, including the
storage case. In one of my patents, I have protected the concept of a “smart storage case”, in
which the outside of the case includes a keypad, reminiscent of a TV remote control, or of a
cell phone. Using this device, a woman user of the NFP (Natural Family Planning)
procedures will have the option of displaying the data, accumulated in her Ovulona device,
on the screen of her TV set, and also of inputting symptometric data correlated with
folliculogenesis.
This way, even if not a computer user, the woman will have access to her stored data, in a
paperless manner. I refer to this as the Ovulovision(TM), to differentiate from the more
sophisticated Ovulograph(TM) for medical professionals (not shown - very different).
Offering to the market of subfertility/infertility the combo of quot;non-smartquot; Ovulona plus quot;smartquot;
storage case is envisaged - before we launch the quot;smartquot; Ovulona technology.
Off-label use by NFP afficionados can be expected, before we address them with the smart
Ovulona... Because bootstrapping can only go so far, we have not yet produced the quot;smartquot;
technology.
5
6. From a trial performed by Dr. Chiara Benedetto, MD, PhD
at University of Turin, Italy using Mark 1 prototype
([DPSOH
Trial performed by
Dr. Chiara Benedetto, MD
at 1st Gynecology Clinic,
U. of Turin, Italy
using Mark 1 prototype
bioZhena
6
7. Above are the morning and evening cyclic profiles from one of several baseline subjects
studied by Dr. Benedetto, who reported the results in the form of raw data. The morning and
evening curves were later superimposed, as shown, on the day of the ovulation marker, and
this superimposition was done by someone other than Dr. Benedetto.
Another gynecologist interpreted the early follicular-phase (pre-ovulatory) data as tracking
the maturation of the dominant follicle (the long-term predictive peak).
The data show a remarkable similarity between the morning and evening cyclic profiles.
The data also suggest that we see evidence of subtle quantitative differences: higher
dominant-follicle-driven data in the evening, and also a properly developed first follicular
wave (in the post-ovulatory part of the profile), with all three waves higher in the PM data
than in the AM data.
While these outcomes are examples of results that “make sense”, we emphasize again that
the independent investigator reported the raw data, and the comparison of the morning and
evening results, as well as of the data from different subjects, was examined later by
someone else. This circumstance (no possible bias or fudging) was one of the factors that
helped me to appreciate what a remarkable technology we had discovered, and to make the
change from an employee of a big pharma firm to an entrepreneur dedicated to the
commercialization of the technology.
Note that we did not have access to baseline subjects prior to the pilot study in Turin. Below
is an example of the in-house data with which the project started, and in which we first
observed the repetitive features of the cyclic pattern. The pattern recognition exercise was
not made particularly easy by the non-baseline character of the first subject(s) of study, but it
is important to note that the differences between baseline and non-baseline are quantitative,
not qualitative, and that the deviations from baseline are explicable.
Below is an example of three non-baseline cycles superimposed in the usual manner.
7
8. One of the three cycles is shown below. The follicle-maturation peak is discernible despite
the missing data points. And we see an example of delayed ovulation (3 days instead of 1).
8
9. Similar non-baseline data were later generated in another pilot study at the Natural Family
Planning Clinic of Marquette University, and an example is shown below.
9
10. Marquette University (Milwaukee, WI) confirmed Turin
results with Mark 3 prototype (digital)
R.J. Fehring and W.D. Schlaff, J. Nurse-Midwifery 43 (2), 117, 1998
bioZhena
10
11. Below is another example of the beauty discovered in the results of the experiments of Dr.
Benedetto. On advice of the late Professor Trevor Slater (doyen of free radical medicine), we
sent several prototypes to the gynecologist Dr. Chiara Benedetto in Turin, for her to test the
technology on baseline subjects. Slater and Benedetto were looking for a better cervical
cancer screen, and this pilot study was designed to produce a baseline for a prospective
investigation of early cancer detection.
Note that we did not have access to baseline subjects prior to this. I also stress again that the
independent obgyn investigator reported raw data. Only later did we perform the comparison
of individual cycles, superimposing the curves on the ovulation marker day.
At the time, there was no interpretation available for the follicular waves in the postovulatory
part (luteal phase) of the profiles. Follicular waves were discovered much later, and for some
time were believed to occur only in animals (separately, we had the waves data from cows
and pigs, initially unaware of the follicular wave interpretation [which was put forward later by
a Kansas veterinary school professor]).
Another gynecologist interpreted for me the dominant follicle maturation meaning of the long-
term predictive peak. The interpretation was assisted by the aberrant results generated by
one of Chiara Benedetto’s baseline subjects, and interpreted as a case of LPD (luteal phase
defect). LPD is known to be a randomly occurring cause of the frequent failure to conceive by
healthy young women. In LPD, the dominant follicle fails to mature, and we detect the failure
early in our measurement results (anticipating the defect that occurs in the second half of the
cycle); see Example 3.
11
13. Example 3
The abnormal cycle record in this example illustrates Ovulona’s capability to recognize
irregularities (here: Ovulona anticipates LPD by detecting the absence of the long-term
predictive peak, which represents the failure of dominant follicle maturation. In the
corresponding BBT curve, the collapse of the rising temperature profile is symptomatic of
LPD.)
13