This document summarizes key information about the enzyme lysozyme. It begins by providing background on lysozyme, including that it is a 129 amino acid enzyme that catalyzes the breakdown of bacterial cell walls. It then discusses the historical discovery of lysozyme by Fleming in 1922 from nasal mucus and its natural occurrence in egg whites, tears, and saliva. The document concludes by summarizing lysozyme's role in disease defense and its importance as one of the first enzyme structures to be solved using X-ray crystallography.
Mechanism of action of Chymotrypsin & Lysozyme.pptxVanshikaVarshney5
Chymotrypsin and Lysozyme are the most important enzymes. Mechanism of action of these enzymes and introduction of these enzyme are given in this presentation in simple, easy and understanding language. Hope you will find it useful :)
Introduction-Cell wall and functions
Gram +ve and -ve cell wall
Bacterial cell wall - structure
Peptidoglycan-Composition and Structure
Types of polysaccharidesBacterial cell wall
Functions of polysaccharides in Bacterial cell wall
Mechanism of action of Chymotrypsin & Lysozyme.pptxVanshikaVarshney5
Chymotrypsin and Lysozyme are the most important enzymes. Mechanism of action of these enzymes and introduction of these enzyme are given in this presentation in simple, easy and understanding language. Hope you will find it useful :)
Introduction-Cell wall and functions
Gram +ve and -ve cell wall
Bacterial cell wall - structure
Peptidoglycan-Composition and Structure
Types of polysaccharidesBacterial cell wall
Functions of polysaccharides in Bacterial cell wall
HMP shunt pathway is a shunt pathway from glycolytic pathway. starting form glucose 6 pasphat by the action of an enzymes known as g6pd. by this pathway an important reducing substance named NADPH2 is produce which result in reducing other substances for its synthesis.
Multienzyme System: A complex of enzymes within a cell that form a reaction sequence of a biochemical pathway so that the product of the first enzyme reaction is transferred directly to the next enzyme and immediately undergoes a second reaction, and so on.
BIOSYNTHESIS OF PHOSPHOLIPIDS
Phospholipids:-
These are compounds containing, in addition to fatty acid and glycerol, phosphoric acid, nitrogenous bases, and another substituent. Polar compounds composed of alcohol attached by phosphodiester bridge to either diacylglycerol or sphingosine.
Amphipathic in nature has a hydrophilic head (phosphate +alcohol
eg., serine, ethanolamine, and choline) and a long, hydrophobic tail
(fatty acids or derivatives ).
- CLASSIFICATION OF PHOSPHOLIPIDS:-
- Glycerophospholipids
- Spingophospholipids or Sphingomyelin
- SYNTHESIS OF PHOSPHOLIPIDS
- FUNCTIONS OF PHOSPHOLIPIDS
- FUNCTIONS OF SPHINGOLIPIDS
HMP shunt pathway is a shunt pathway from glycolytic pathway. starting form glucose 6 pasphat by the action of an enzymes known as g6pd. by this pathway an important reducing substance named NADPH2 is produce which result in reducing other substances for its synthesis.
Multienzyme System: A complex of enzymes within a cell that form a reaction sequence of a biochemical pathway so that the product of the first enzyme reaction is transferred directly to the next enzyme and immediately undergoes a second reaction, and so on.
BIOSYNTHESIS OF PHOSPHOLIPIDS
Phospholipids:-
These are compounds containing, in addition to fatty acid and glycerol, phosphoric acid, nitrogenous bases, and another substituent. Polar compounds composed of alcohol attached by phosphodiester bridge to either diacylglycerol or sphingosine.
Amphipathic in nature has a hydrophilic head (phosphate +alcohol
eg., serine, ethanolamine, and choline) and a long, hydrophobic tail
(fatty acids or derivatives ).
- CLASSIFICATION OF PHOSPHOLIPIDS:-
- Glycerophospholipids
- Spingophospholipids or Sphingomyelin
- SYNTHESIS OF PHOSPHOLIPIDS
- FUNCTIONS OF PHOSPHOLIPIDS
- FUNCTIONS OF SPHINGOLIPIDS
Niosomes, Aquasomes, Phytosomes,Electrosomes Molecular pharmaceutics (MPH 201T) PRESENTATION BY- NARAYAN R KOTE M PHARM [PHARMACEUTICS] ROLL NO. 8 GUIDANCE BY :- Dr . TIWARI S. S
CONTENTS
NIOSOMES
AQUASOMES
PHYTOSOMES
ELECTROSOMES
NIOSOMES
Niosomes are a novel drug delivery system, in which the medication is encapsulated in a vesicle. The vesicle is composed of a bilayer of non-ionic surface active agents and hence the name niosomes.
The niosomes are very small, and microscopic in size.
Their size lies in the nanometric scale. Although structurally similar to liposomes, they offer several advantages over them.
Niosomes have recently been shown to greatly increase transdermal drug delivery and also can be used in targeted drug delivery, and thus increased study in these structures can provide new methods for drug delivery.
STRUCTURE OF NIOSOMES
Structurally, niosomes are similar to liposomes, in that they are also made up of a bilayer.
However, the bilayer in the case of niosomes is made up of non-ionic surface active agents rather than phospholipids as seen in the case of liposomes.
Most surface active agents when immersed in water yield micellar structures however some surfactants can yield bilayer vesicles which are niosomes.
STRUCTURE OF NIOSOMES
Structurally, niosomes are similar to liposomes, in that they are also made up of a bilayer.
However, the bilayer in the case of niosomes is made up of non-ionic surface active agents rather than phospholipids as seen in the case of liposomes.
Most surface active agents when immersed in water yield micellar structures however some surfactants can yield bilayer vesicles which are niosomes.
APPLICATION OF NIOSOMES
Drug Targetting
One of the most useful aspects of niosomes is their ability to target drugs.
Niosomes can be used to target drugs to the reticuloendothelial system.It can be achieved by coating with polymer e.g. PEG.
In Diagnosis
Niosomes have also been used as carriers for iobitridol, a diagnostic agent used for X-ray imaging.
Anti-neoplastic Treatment
Most antineoplastic drugs cause severe side effects.
Niosomes can alter the metabolism; prolong circulation and half life of the drug, thus decreasing the side effects of the drugs.
Niosomes, is decreased rate of proliferation of tumor and higher plasma levels accompanied by slower elimination.Leishmaniasis :-
Leishmaniasis is a disease in which a parasite of the genus Leishmania invades the cells of the liver and spleen.
Use of niosomes in tests conducted showed that it was possible to administer higher levels of the drug without the triggering of the side effects, and thus allowed greater efficacy in treatment.
Delivery of Peptide Drugs:-
Oral peptide drug delivery has long been faced with a challenge of bypassing the enzymes which would breakdown the peptide.
Use of niosomes to successfully protect the peptides from gastrointestinal peptide breakdown is being investigated.
In an in-vitro study conducted by ODDS.
Excretion in various invertebrates and vertebrates is dealt as per the PG syllabus prescribed by Vijayanagara Sri Krishnadevaraya University Ballari. It is useful for the PG students studying animal physiology as the core subject under zoology. It gives a overall picture of excretion to the teacher who is teaching animal physiology to the collegiate and university levels.
Osmosis role in Animal cell describe the nature of cells in different solutions such as Hypertonic, Hypotonic and Isotonic medium cells become become Plasmolysed, Turgid and No change respectively.
Executive Directors Chat Leveraging AI for Diversity, Equity, and InclusionTechSoup
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it describes the bony anatomy including the femoral head , acetabulum, labrum . also discusses the capsule , ligaments . muscle that act on the hip joint and the range of motion are outlined. factors affecting hip joint stability and weight transmission through the joint are summarized.
2024.06.01 Introducing a competency framework for languag learning materials ...Sandy Millin
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Published classroom materials form the basis of syllabuses, drive teacher professional development, and have a potentially huge influence on learners, teachers and education systems. All teachers also create their own materials, whether a few sentences on a blackboard, a highly-structured fully-realised online course, or anything in between. Despite this, the knowledge and skills needed to create effective language learning materials are rarely part of teacher training, and are mostly learnt by trial and error.
Knowledge and skills frameworks, generally called competency frameworks, for ELT teachers, trainers and managers have existed for a few years now. However, until I created one for my MA dissertation, there wasn’t one drawing together what we need to know and do to be able to effectively produce language learning materials.
This webinar will introduce you to my framework, highlighting the key competencies I identified from my research. It will also show how anybody involved in language teaching (any language, not just English!), teacher training, managing schools or developing language learning materials can benefit from using the framework.
Read| The latest issue of The Challenger is here! We are thrilled to announce that our school paper has qualified for the NATIONAL SCHOOLS PRESS CONFERENCE (NSPC) 2024. Thank you for your unwavering support and trust. Dive into the stories that made us stand out!
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Acetabularia acetabulum is a single-celled green alga that in its vegetative state is morphologically differentiated into a basal rhizoid and an axially elongated stalk, which bears whorls of branching hairs. The single diploid nucleus resides in the rhizoid.
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This slides describes the basic concepts of ICT, basics of Email, Emerging Technology and Digital Initiatives in Education. This presentations aligns with the UGC Paper I syllabus.
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Lysozyme by devadatha
1. Submitted by;
B.Devadatha
M.Sc2nd sem
Dept of Biochemistry
and Molecular Biology.
Submitted to;
Dr. R. Rukkumani,
Assistant Professor,
Dept of Biochemistry
and Molecular Biology.
LYSOZYME
2. Lysozyme: is 129 aminoacid residues enzyme
(EC 3.2.1.17), hydrolase which catalyzes hydrolysis of 1,4-
beta-linkages between N-acetylmuramic acid and N-acetyl-D-
glucosamine residues in peptidoglycan and between N-acetyl-
D-glucosamine residues in chitodextrins.
Molecular weight of Lysozyme is an approximately 14.7 kDa.
LYSOZYME
3. An enzyme found naturally in
• egg white
• human tears
• saliva (as well as other body fluids)
Capable of destroying the cell walls of certain
bacteria.
Acts as a mild antiseptic
OCCURENCE
4. Historical background
Lysozyme was the first enzyme structure solved with X-ray
crystallography (Structure 2LYZ in the PDB, deposited 1975 by
Diamond and Phillips). It is now the most common enzyme in the
PDB (966 structures), most of which are engineered mutants from
the work of Matthews .
Lashtchenko (1909) observed the antibacterial
properties of hen egg white. Hen egg white lysozyme
(HEWL) has become the “classic” lysozyme.
Fleming (1922) noted the antibacterial property of
nasal mucus.
5. FLEMINGS DISCOVERY OF LYSOZYME
The lysozyme was first noticed during some investigations made on
a patient suffering from acute coryza
The nasal secretion of this patient was cultivated daily on blood
agar plates and for the first three days of the infection there was no
growth, with the exception of an occasional staphylococcus colony
The culture made from the nasal mucus on the fourth day showed in
24 hours a large number of small colonies which, on examination
proved to be large gram-positive cocci .
The microbe has not been exactly identified alluded as
Micrococcus lysodeikticus.
6. PRELIMINARY EXPERIMENTS SHOWING THE ACTION
OF THE LYSOZYME
Nasal mucus from the patient, with coryza, was
shaken up with five times its volume of normal salt solution, and the
mixture was centrifuged.
A drop of the clear supernatant fluid was placed
on an agar plate, which had previously been thickly planted with M.
lysodeikticus, and the plate was incubated at 37° C. for 24 hours.
It showed a copious growth of the coccus, except in the region where
the nasal mucus has been placed.
There was complete inhibition of growth, and this inhibition extended
for a distance of about 1 cm. Beyond the limits of the mucus.
7. The effect of the lysozyme on bacteria
Inhibitory Action:
A small portion of the agar is removed from
an ordinary agar plate making a cup into
which some material rich in lysozyme
A drop of liquid agar, at a temperature
of about 50° C., is placed on the material in
the cup and is allowed to solidify after
which the cup is filled with the liquid agar
Liquid agar is then poured all over the plate
to make a thin layer over the original
surface
8. • The whole surface of the medium is now
thickly planted with the M. lysodeikticus and
the plate is incubated for 24 hours.
• when it will be seen that there is copious
growth of the coccus except in the region of
the implanted material.
• It is evident that the lytic substance has
dissolved the cocci for a distance of 3 or 4
mm.
9. Properties of lysozyme
Lysozyme has the characters of a ferment. The rapidity of its
action increases up to 60° C, but at temperatures over 65° C. it
is destroyed more or less rapidly.
It acts best in a neutral medium.
Peptic or tryptic digestion does not destroy lysozyme.
Stability—When kept dry, lysozyme can be preserved for a
long time. It was noted that commercial dried egg albumen
was very rich in lysozyme .
10. Distribution of lysozyme
In the human body:- Many tissues and secretions have been
examined for lysozyme .
Of the secretions and body-fluids, all contained lysozyme except
normal urine, sweat, and cerebrospinal fluid.
In the tissues of animals :- Florey, who titrated the lysozyme
content of extracts of many tissues of several animals and
compared them with human tissues.
Cat’s tissues, with the striking exception of the salivary gland,
are relatively deficient in lysozyme.
11. CONCLUSION
lysozyme is a widely distributed antibacterial ferment which is
probably inherent in all animal cells and constitutes a primary
method of destroying bacteria
while acting most strikingly on non-pathogenic bacteria yet can,
when allowed to act in the full strength in which it occurs in
some parts of the body, attack pathogenic organisms.
12. That it is very easy to make bacteria relatively resistant to
lysozyme, so that any pathogenic microbe isolated from the
body where it has been growing in the presence of a non-lethal
concentration of lysozyme must have acquired increased
resistance to the ferment.
There are some differences in the lysozyme of different tissues
and in different animals whereby bacteria are susceptible to
different lysozymes in varying degrees.
CONCLUSION
13. The Hen Egg-white Lysozyme Molecule
The three-dimensional structure of the hen egg-white lysozyme molecule
has been determined by X-ray analysis of the tetragonal crystals grown at
pH 4.7 from 1 M sodium chloride solution.
The phases of the X-ray reflections were determined by the method of
multiple isomorphous replacement developed to take systematic account
of anomalous scattering effects .
It has been interpreted with reference to the known amino acid sequence
of lysozyme as determined independently by Jolls and Canfield and their
colleagues.
crystals of Lysozyme diffract X-Ray beam to a very high resolution,
currently the highest resolution structure, presented in Protein Data
Bank, was solved at resolution 0.94 Angstrom
14.
15. Interpretation of the Electron Density
Map
• Hen egg-white lysozyme has a molecular weight of
about 14,600 and each molecule comprises 129
amino acid residues.
• The region of highest electron density, at lower right
of center, was found to correspond to the half-
cysteine residue 30.
• since it clearly forms part of a helical conformation
of the polypeptide chain, the axis of which is marked
HH‘
• Four residues removed from an identifiable
phenylalanine residue (34) nearer the carboxyl end
of the chain
16.
17. Role in disease
• Lysozyme is part of the innate immune system.
• Reduced lysozyme levels have been associated
with broncho pulmonary dysplasia in newborns.
• Since lysozyme is a natural form of protection from
gram-positive pathogens
like Bacillus and Streptococcus
• In certain cancers (especially myelomonocytic
leukemia) excessive production of lysozyme by
cancer cells can lead to toxic levels of lysozyme in
the blood.
• High lysozyme blood levels can lead to kidney
failure and low blood potassium.
18. Reference
• On a Remarkable Bacteriolytic Element
found in Tissues and Secretions.
• By ALEXANDER FLEMING, M.B.,F.R.C.S.
• THE HEN EGG-WHITE LYSOZYME MOLECULE
• BY DAVID C. PHILLIPS