Auxin is the first plant hormone discovered. It is produced throughout the plant and regulates many growth processes. There are two main pathways for auxin (IAA) biosynthesis - tryptophan-dependent and tryptophan-independent. Auxin's mechanism of action involves binding to receptor proteins and promoting proton pumping, which acidifies the cell wall and activates expansin proteins, leading to cell wall loosening and elongation. The physiological effects of auxin include stimulating cell elongation, controlling apical dominance, initiating root formation, preventing abscission, and promoting callus growth and vascular differentiation.

1. PLANT HORMONE :AUXIN

2. CONTENTS
• Introduction of plant hormones
• History of Auxin
• Structure of Auxin
• Biosynthesis of Auxin
• Mechanism of Auxin action
• Physiological effects of Auxin

3. Introduction
• Plant hormones :
Plant hormones are regulators
produced by plant which in low concentration
regulate a Physiological plant processes.
• Hormones usually move within plant form a site of
production to site of action.
• Thimann (1948) suggested using the term
“Phytohormone” for hormones of plant.

4. Classification
• It is accepted that there are two major classes of
plant hormones :
1.Growth Pramotors :Auxins
Cytokinins
Gibberellins
2.Growth Inhibitors : Ethylene
Abscisic Acid (ABA)
Jasmonic Acid

5. History
• Auxins were the first hormones discovered.
• Charles Darwin was among the first scientists to
dabble in plant hormone reaserch.
• In his book “The Power of Movement In Plants”
presented in 1980 .
• The term “Auxin” is derived from the greek word “to
increase or grow”.
• This was the first group of plant hormones
discovered.

6. Structure of Auxins

7. Biosynthesis of Auxin
• There is a two pathways in Biosynthesis of
Auxin :
1.Tryptophan dependent pathway
-IPA Pathway
-TAM Pathway
-IAN Pathway
-Bacterial pathway
2.Tryptophan Independent pathway

8. (A)TRYPTOPHAN DEPENDENT PATHWAYS
 In 1935,Thimann demostrated that a fungus
Rhizopus suinus could convert an amino acid
tryptophan (trp) into indole-3-acetic acid (IAA).
 Since then,it is generally held that tryptophan
is primary precursor of IAA in plants.
 The indole-3-acetic acid(IAA) can be formed
from tryptophan by different pathways.

10. a.IPA(Indole-3-pyruvic acid) pathway
Tryptophan is determinated to form indole-
3-pyruvic acid(IPA) followed by decarboxylate
of the latter resulting in the formation of
indole-3-acetaldehyde(Iald).
 The enzymes involved are tryptophan
transminase and indole pyruvate
decarboxylase.
 One of the above two methods (sometimes
both) is most common pathway of formation
of IAA in plants.

11. b.TAM(Tryptamine) pathway
Tryptophan is decarboxylated to form
tryptamine followed by deamination of the
latter resulting in the formation of indole-3-
acetaldehyde (Iald).
 The enzymes involved are tryptophan
decarboxylase and tryptamine oxidase
respectively.
 Iald is readily oxidised to indole-3-acetic acid
(IAA) by the enzyme Iald dehydrogenase.

12. c. IAN (Indole-3-acetonitrile) pathway
It occurs in some plants especially those
belonging to families Brassicaceae,Poaceae
and Musaceae.
 Tryptophan is converted into IAA in the
presence of the enzyme nitrilase.
 Indole-3-acetaldoxime and indole -3-
acetonitrile(IAN) are the intermidiates.

13. d. Bacterial pathway
In some pathogenic bacteria such as
Agrobacterium tumefaciens and Pseudomonas
savastanoi, tryptophan is first converted into
indole-3-acetamine (IAM) in the presence of
tryptophan monooxygenase.
 IAM is then hydrolysed to IAA in the presence of
the enzyme IAM hydrolyse.
 The auxin(IAA) produced in this way often
causes morphological changes in the host plant
cells.

14. (B) TRYPTOPHAN INDEPENDENT PATHWAYS
In recent years, experimental evidences for
the existance of tryptophan independent
pathways of IAA biosynthesis in higher plants
have been obtained from mutants of maize
and Arabidopsis (family Brassicaceae).
 The branch point for biosynthesis of IAA may
be either indole or indole-3-glycerol
phosphate with IAN and IPA as the possible
intermidiates.

15. However, neither the immediate precursor of
IAA in this pathway has yet been
identified,not relative importance of
tryptophan dependent and independent
pathways is clearly understood.

16. Mechanism action of auxin
• Auxin(IAA) causes wide range of physiological
effects in plants.
• Some of these effects such as cell elongation
in shoot occur in minutes in response to auxin
while others such as abscission occur in days
in response to auxin treatment.
• Most studies of auxin induced growth in
plants have been carried out on excised
sections of dicot stems(such as soyabean
hypocotyls and pea epicotyls).

17. • And coleoptile such as oat(Avena) and maize
coleoptile.
• But , in recent years auxin- induced growth has
also been demonstrated in intact auxin-deficient
mutants of pea.
• The target sites of auxin action in dicot stems are
the outer tissue including epidermis and outer
cortex.
• In coleoptile, all non vascular tissues
I.e.,epidermis and mesophyll are responsive to
auxin treatment.

18. 1.Presence of auxin-binding receptor :
A possible auxin-binding receptor
protein has been identified in plants which is called
as auxin-binding protein 1 (ABP 1).
• This protein appers to be a dimer made of two
polypeptides of about 22 kD each.
• ABP 1 is located in lumen of endoplasmic
reticulum(ER) , but it is belived to be active on
surface cell.

19. 2.Minimum lag time for auxin induced growth
is 10 minutes :
When coleoptile or stem sections
are excised and placed in sensitive growth
measuring device, the growth response to
auxin can be measured with high degree of
precision.
• If auxin is absent in the medium, the growth
rate declines rapidly.

20. • Addition of auxin on the other hand, markedly
stimulates growth rate (measured in terms of
elongation or % increase in length) after a lag
period of 10-12 minutes only.
• In both types of tissues the maximum growth
is attained after half an hour to one hour of
auxin treatment.

21. 3.Auxin causes Rapid increase in cell wall
Extensibility :
Cell wall enlargement in plants
involves two steps ,(1)Osmatic uptake of water
across the plasma membrane resulting in
increased turgor pressure of the cell and
(2)Extension of cell wall in response to increased
turgor pressure.
• It is generally believed that auxin causes an
increase in plastic(i.e.,irreversible) extensibility of
the cell wall by wall loosening events that require
continuous input of metabolic energy.

22. 4.Auxin –Induced oroton (H+) extrusion
acidifies the cell wall , resulting in wall
loosening-the acid growth hypothesis :
Rayle and Cleland (1970) in USA and
Hager,Manzel and Cross (1971) in Germany
first proposed independently that protons(H+)
may be involved in Auxin-induced cell wall
loosening.
• Auxin causes responsive cells to extrude
protons(H+ ions) actively from cytoplasm to
cell wall resulting in decrease of cell wall pH.

23. • The law apoplastic pH activates cell wall
loosening enzymes which break the load-bearing
bounds, thus increasing extensibility of the cell
wall and the extrusion of protons is faciliated by
H+- ATPases located in plasma-membrane.
• 5.Acid induced wall loosening is mediated by
specific proteins called expansins :
Previously,hydrolytic enzymes such as
celluloses,hemi-celluloses and pectinases were
considered as well loosening enzymes which
were activated by low pH during auxin-induced
growth.

24. • But, while these enzymes loosen cell walls
irreversibly , the auxin-induced growth is
reversible with metabolic inhibitors.
• Therefore, these hydrolytic enzymes are not
involved in wall-loosening during auxin-induced
growth.
• There are now compelling evidences to suggest
that a group of cell wall proteins called
expansions causes cell wall loosening in
response to acidic pH.

25. 6.Auxin maintains the capacity for acid
induced wall loosening by synthesis of new
wall polysaccharides :
Auxin-induced Acidification and loosening
of cell walls is accompanied by biosynthesis of
new wall polysacchrides so that growth may
continue for longer period especially in
coleoptile.
Auxin is known to increase activites of certain
enzymes which are involved in biosynthesis of
cell wall polysacchrides.

26. • A constant supply of new cell materials
maintains the capacity for acid induced wall
loosening (CAWL) in response to auxin.
• Cell walls of sections treated with auxin have
grater capacity for acid stimulated growth
than these of control sections or sections
treated with fungal phytotoxin fusicoccin.
• Polysacchrides synthesis and CAWL do not
correlate well with rapid kinetics of growth
and are therefore, parts of long term growth
responses to auxin.

27. Physiological Effects Of Auxin
• The following points highlight the physiological
effects of auxin in plants.
1.Cell Elongation
2.Apical Dominance
3.Root Initiation
4.Prevention of Abscission
5.Parthenocarpy
6.Respiration
7.Callus Formation
8.Vascular Differentiation.

28. 1.Cell Elongation
• The primary physiological effect of auxin in plants is
to stimulate the elongation of cells in shoot.
• A very common example of this can be observed in
phototropic curvatures where the unilateral light
unequally distributes the auxin in the stem tip
(i.e.,More auxin on shaded side that on illuminated
side).
• Many theories have been proposed to explain the
mechanism of cell elongation probably :

29. By reducing the wall pressure,
By increasing the permeability of cells to water,
By an increase in the wall synthesis and,
By inducing the synthesis of RNA and Protein which
turn lead to an increase in cell wall plasticity and
extension.

30. 2.Apical Dominance
• Apical or terminal buds of many vascular plants are
very active while the lateral buds remain inactive.
• Removal of apical buds promotes lateral buds to
grow.
• Apical dominance is due to much higher auxin
content in the apical buds than lateral buds.
• Skoog and Thimann(1934) first pointed out that the
apical dominance might be under the control of
auxin produced at the terminal bud and which is
transported downward through the stem to the

31. Lateral buds and hinders their growth.
• They removed the apical bud of broad bean plant
and replaced it with agar block.
• This resulted in rapid growth of lateral buds.
• But ,when they replaced the apical bud with agar
block containing auxin lateral buds remained
suppressed and did not grow.
• It is now generally held that inhibitory effect of
auxin from shoot apex on lateral buds is not direct
but in indirect possibly through the involvement of
other growth hormones such as cytokinins & ABA.

32. 3.Root Initiation
• In contrast to the stem,the higher concentration of
inhibits the elongation of root but the number of
lateral branch roots is considerably increased i.e.
the higher conc. of auxin initiates more lateral
branch roots.
• Application of IAA in lanolin paste to the cut end
of a young stem results is an early and extensive
rooting.
• This fact is of great practical importance and has
been widely utilised to promote root formation in
economically useful plants which are propagated
by cuttings.

33. 4.Prevention of Abscission
• Natural auxins have controlling influence on the
abscission of leaves , fruits etc..

34. 5.Parthenocarpy
• Auxin can induce the formation of parthenocarpic
fruits.
• In nature also,this phenomenon is not uncommon
and in such cases the concentration of auxins in
the ovaries has been found to be higher than in
the ovaries of plants which produce fruits only
after fertilization.
• In the latter cases, the concentration of the auxin
in ovaries increases after pollination and
fertilization.

35. 6.Respiration
• It has been established that the auxin stimulates
respiration and there is a correlation between
auxin induced growth and an increased
respiration rate.
• According to French and Beevers (1953),the
auxin may increase the rate of respiration
indirectly though increased supply of
ADP(Adenosine diphosphate) by rapidly utilizing
the ATP in the expanding cells.

36. 7.Callus Formation
• Besides cell elongation the auxin may also be
active in cell division.
• In fact,in many tissue cultures where the callus
growth is quite normal, the continued growth of
such callus takes place only after the addition of
auxin.

37. 8.Vascular Difference
• Auxin induces vascular differentiation in plant.
• This has also been confirmed in tissue culture
experiments and form studies with transgenic
plants.
• Cytokinins are also known to participate in
differentiation of vascular tissues and it is belived
that vascular differentiation in plants is probably
under the control of both auxin and cytokinins.

38. Reference
• Fundamentals of Plant Physiology – V.K.JAIN.
• Figure Reference – TAIZ & ZEIGER

39. Thank you