Genetic engineering techniques can be used to improve plants for agriculture. Methods like Agrobacterium transfer and biolistic bombardment are used to insert genes. This allows traits like insect and disease resistance, herbicide tolerance, nutritional enhancement, and more. However, there are also risks to the environment and human health that must be considered through safety testing and regulation. While GM crops have potential benefits, caution is needed to avoid unintended harm.
Insect-resistant transgenic crops were first commercialized in the mid-1990s with the introduction of GM corn (maize), potato and cotton plants expressing genes encoding the entomocidal δ-endotoxin from Bacillus thuringiensis (Bt; also known as Cry proteins). In 2010, 148 million ha of biotech crops were grown in 29 countries, representing 10% of all 1.5 billion hectares of cropland in the world. The global value of this seed alone was valued at US $11.2 billion in 2010, with commercial biotech maize, soybean grain and cotton valued at approximately US $150 billion per year. In recent years, it has become evident that Bt-expressing crops have made a significant beneficial impact on global agriculture, not least in terms of pest reduction and improved quality. However, because of the potential for pest populations to evolve resistance, and owing to lack of effective control of homopteran pests, alternative strategies are being developed. Some of these are based on Bacillus spp., e.g. vegetative insecticidal proteins (VIPs) or other insect pathogens.
An overview of the Agrobacterium-mediated gene transfer process. Moreover, studied different kinds of Agrobacterium species are involved in this mechanism.
Agrobacterium is a rod-shaped, Gram-negative bacteria found mostly in the soil. It is a plant pathogen that is responsible for causing crown gall disease in them. This bacteria is also known as the natural genetic engineer because of it's the ability to integrate its plasmid Gene into the plant genome.
Agrobacterium tumefaciens transfer of their genetic material T-DNA of Ti-plasmid into the plant cell: A: Agrobacterium tumefaciens; B: Agrobacterium genome; C: Ti Plasmid : a: T-DNA , b: Vir genes , c: Replication origin , d: Opines catabolism genes; D: Plant cell
A Ti-Plasmid (tumor-inducing plasmid) is a ds, circular DNA that often, but not always. It's a piece of genetic equipment that transfers genetic material from bacterial cells means Agrobacterium tumefaciens into plant cells used to induce tumors in the plant. The Ti-plasmid is damage when Agrobacterium is grown above 28 °C. Such cured bacteria don't induce crown gall disease in the plant due to they are avirulent. The Ti-Plasmid are classified into two types on the basis of opine genes are present in T-DNA.
The Plasmid has 196 genes that code for 195 proteins. There is no one structural RNA. The plasmid is 206.479 nucleotides long. the GC content is 56% and 81% of the genetic material is coding genes.
The modification of this plasmid is a very important source in the production of transgenic plants.
The T-DNA must be cut out of the circular plasmid. A VirD1/D2 complex nicks the DNA at the left and right border sequences. The VirD2 protein is covalently attached to the 5' end. VirD2 contains a motif that leads to the nucleoprotein complex being targeted to the type IV secretion system (T4SS).
In the cytoplasm of the recipient cell, the T-DNA complex becomes coated with VirE2 proteins, which are exported through the T4SS independently from the T-DNA complex. Nuclear localization signals, or NLS, located on the VirE2 and VirD2 are recognized by the importin alpha protein, which then associates with importin beta and the nuclear pore complex to transfer the T-DNA into the nucleus. So that the T-DNA can integrate into the host genome.
We inoculate Agrobacterium containing our genes of interest, onto wounded plant tissue explants. The Agrobacterium then transfers the gene of interest into the DNA of the plant tissue.
In the following slides, I have discussed the need for developing insect-resistant transgenic plants, the sources of transgenes, and methods for development
Insect-resistant transgenic crops were first commercialized in the mid-1990s with the introduction of GM corn (maize), potato and cotton plants expressing genes encoding the entomocidal δ-endotoxin from Bacillus thuringiensis (Bt; also known as Cry proteins). In 2010, 148 million ha of biotech crops were grown in 29 countries, representing 10% of all 1.5 billion hectares of cropland in the world. The global value of this seed alone was valued at US $11.2 billion in 2010, with commercial biotech maize, soybean grain and cotton valued at approximately US $150 billion per year. In recent years, it has become evident that Bt-expressing crops have made a significant beneficial impact on global agriculture, not least in terms of pest reduction and improved quality. However, because of the potential for pest populations to evolve resistance, and owing to lack of effective control of homopteran pests, alternative strategies are being developed. Some of these are based on Bacillus spp., e.g. vegetative insecticidal proteins (VIPs) or other insect pathogens.
An overview of the Agrobacterium-mediated gene transfer process. Moreover, studied different kinds of Agrobacterium species are involved in this mechanism.
Agrobacterium is a rod-shaped, Gram-negative bacteria found mostly in the soil. It is a plant pathogen that is responsible for causing crown gall disease in them. This bacteria is also known as the natural genetic engineer because of it's the ability to integrate its plasmid Gene into the plant genome.
Agrobacterium tumefaciens transfer of their genetic material T-DNA of Ti-plasmid into the plant cell: A: Agrobacterium tumefaciens; B: Agrobacterium genome; C: Ti Plasmid : a: T-DNA , b: Vir genes , c: Replication origin , d: Opines catabolism genes; D: Plant cell
A Ti-Plasmid (tumor-inducing plasmid) is a ds, circular DNA that often, but not always. It's a piece of genetic equipment that transfers genetic material from bacterial cells means Agrobacterium tumefaciens into plant cells used to induce tumors in the plant. The Ti-plasmid is damage when Agrobacterium is grown above 28 °C. Such cured bacteria don't induce crown gall disease in the plant due to they are avirulent. The Ti-Plasmid are classified into two types on the basis of opine genes are present in T-DNA.
The Plasmid has 196 genes that code for 195 proteins. There is no one structural RNA. The plasmid is 206.479 nucleotides long. the GC content is 56% and 81% of the genetic material is coding genes.
The modification of this plasmid is a very important source in the production of transgenic plants.
The T-DNA must be cut out of the circular plasmid. A VirD1/D2 complex nicks the DNA at the left and right border sequences. The VirD2 protein is covalently attached to the 5' end. VirD2 contains a motif that leads to the nucleoprotein complex being targeted to the type IV secretion system (T4SS).
In the cytoplasm of the recipient cell, the T-DNA complex becomes coated with VirE2 proteins, which are exported through the T4SS independently from the T-DNA complex. Nuclear localization signals, or NLS, located on the VirE2 and VirD2 are recognized by the importin alpha protein, which then associates with importin beta and the nuclear pore complex to transfer the T-DNA into the nucleus. So that the T-DNA can integrate into the host genome.
We inoculate Agrobacterium containing our genes of interest, onto wounded plant tissue explants. The Agrobacterium then transfers the gene of interest into the DNA of the plant tissue.
In the following slides, I have discussed the need for developing insect-resistant transgenic plants, the sources of transgenes, and methods for development
introduction
What is virus
What is virus resistance plant
History
Gene use for develop virus resistance plant
Coat protein gene
cDNA of satellite RNA
Defective viral genome
Antisense RNA approach and
Ribozyme – mediated protection
conclusion
References
The different types of external stresses that influence the plant growth and development.
These stresses are grouped based on their characters
Biotic
Abiotic
Almost all the stresses, either directly or indirectly, lead to the production of reactive oxygen species (ROS) that create oxidative stress in plants.
This damages the cellular constituents of plants which are associated with a reduction in plant yield.
The advances of modern plant technologies, especially genetically modified crops, are considered to be a substantial benefit to agriculture and society. However, so-called transgene escape remains and is of environmental and regulatory concern. Genetic use restriction technologies (GURTs), developed to secure return on investments through protection of plant varieties, are among the most controversial and opposed genetic engineering biotechnologies as they are perceived as a tool to force farmers to depend on multinational corporations’ seed monopolies. In this work, the currently proposed strategies are described and compared with some of the principal techniques implemented for preventing transgene flow and/or seed saving, with a simultaneous analysis of the future perspectives of GURTs taking into account potential benefits, possible impacts on farmers and local plant genetic resources (PGR), hypothetical negative environmental issues and ethical concerns related to intellectual property that have led to the ban of this technology
Presented by- MD JAKIR HOSSAIN
Doctoral Research Scholar
Department of Agricultural Genetic Engineering ,
Faculty of Agricultural Sciences and Technologies,
Nigde Omer Halisdemir University, Turkey
E. Mail- mjakirbotru@gmail.com
Plant Genetic engineering ,Basic steps ,Advantages and disadvantagesTessaRaju
plant genetic engineering,first genetically engineered crop plant,first genetically engineered foods,genome editing,uses of GE,transgenic plants,basic process of plant genetic enginering,advantages and disadvantages of genetic engineering.
This presentation entitled "Golden rice" explains the needs for golden rice development, Biotechnological manipulations in metabolic pathways for GR-1 and GR-2 development and finally it also detailed with the associated ethical issues.
introduction
What is virus
What is virus resistance plant
History
Gene use for develop virus resistance plant
Coat protein gene
cDNA of satellite RNA
Defective viral genome
Antisense RNA approach and
Ribozyme – mediated protection
conclusion
References
The different types of external stresses that influence the plant growth and development.
These stresses are grouped based on their characters
Biotic
Abiotic
Almost all the stresses, either directly or indirectly, lead to the production of reactive oxygen species (ROS) that create oxidative stress in plants.
This damages the cellular constituents of plants which are associated with a reduction in plant yield.
The advances of modern plant technologies, especially genetically modified crops, are considered to be a substantial benefit to agriculture and society. However, so-called transgene escape remains and is of environmental and regulatory concern. Genetic use restriction technologies (GURTs), developed to secure return on investments through protection of plant varieties, are among the most controversial and opposed genetic engineering biotechnologies as they are perceived as a tool to force farmers to depend on multinational corporations’ seed monopolies. In this work, the currently proposed strategies are described and compared with some of the principal techniques implemented for preventing transgene flow and/or seed saving, with a simultaneous analysis of the future perspectives of GURTs taking into account potential benefits, possible impacts on farmers and local plant genetic resources (PGR), hypothetical negative environmental issues and ethical concerns related to intellectual property that have led to the ban of this technology
Presented by- MD JAKIR HOSSAIN
Doctoral Research Scholar
Department of Agricultural Genetic Engineering ,
Faculty of Agricultural Sciences and Technologies,
Nigde Omer Halisdemir University, Turkey
E. Mail- mjakirbotru@gmail.com
Plant Genetic engineering ,Basic steps ,Advantages and disadvantagesTessaRaju
plant genetic engineering,first genetically engineered crop plant,first genetically engineered foods,genome editing,uses of GE,transgenic plants,basic process of plant genetic enginering,advantages and disadvantages of genetic engineering.
This presentation entitled "Golden rice" explains the needs for golden rice development, Biotechnological manipulations in metabolic pathways for GR-1 and GR-2 development and finally it also detailed with the associated ethical issues.
Introduction: Biotechnology is an emerging field of research as it has the potential to solve many biological problems which could not be solved till now with conventional techniques.
The use of biology to develop technologies and products for the welfare of human beings is known as Biotechnology. It has various applications in different fields such as Therapeutics, Diagnostics, Processed Food, Waste Management, Energy Production, Genetically Modified Crops etc.
Biotechnology means 'applications of scientific and engineering principles to biological processes to provide goods and services'. Full understanding of biological processes is possible with detailed analysis of gene structure and function i.e. the Genetic Engineering means the introduction of manipulated genetic material (DNA) into a cell in such a way as to replicate and be passed on to progeny cells'. The outcome is attractive and promising.
Genetic Engineering in Insect Pest management Mohd Irshad
gene incorporation is gaining attention across the globe with the aim of improving plant health, crop protection, and sustainable crop production. This versatile method of Scientific cultivation should be adopted by the growers as it has been investigated and assessed by experts and environmentalists. There is not any kind of toxic effect on mammalian.
Genetically modified organisms (GMOs) are organisms in which the
genetic material has been altered using recombinant DNA technology.
Genetic manipulation involves a wide variety of modifications to produce
nutritionally valued GM crops. In some cases, genetic modifications
represent more faster and efficient mechanisms for achieving desired
resulting traits. This review indicate the mechanism of group of actions
with various biotechnological tool utilize to carry out genetic
modification, their benefits, etc. Production of GM food crops provides
new ways to fulfill future food requirments but risk associated factors
cannot be neglected. To overcome these problems and to cope with the
continuous increase in the number and variety of GMOs, new approaches
are needed. India has approved cultivation of some GM crops but due to
lack of proper knowledge and religious factors lead to stunted outcomes
ignoring environment cleanliness and hunger of malnourished segments.
So more attention still needed for its adoption globally by ensure its
safety for human utilization.
The presentation is about the introduction, usage, benefits and disadvantages of biological techniques through we are producing genetically modified foods
Transcript: Selling digital books in 2024: Insights from industry leaders - T...BookNet Canada
The publishing industry has been selling digital audiobooks and ebooks for over a decade and has found its groove. What’s changed? What has stayed the same? Where do we go from here? Join a group of leading sales peers from across the industry for a conversation about the lessons learned since the popularization of digital books, best practices, digital book supply chain management, and more.
Link to video recording: https://bnctechforum.ca/sessions/selling-digital-books-in-2024-insights-from-industry-leaders/
Presented by BookNet Canada on May 28, 2024, with support from the Department of Canadian Heritage.
Smart TV Buyer Insights Survey 2024 by 91mobiles.pdf91mobiles
91mobiles recently conducted a Smart TV Buyer Insights Survey in which we asked over 3,000 respondents about the TV they own, aspects they look at on a new TV, and their TV buying preferences.
GDG Cloud Southlake #33: Boule & Rebala: Effective AppSec in SDLC using Deplo...James Anderson
Effective Application Security in Software Delivery lifecycle using Deployment Firewall and DBOM
The modern software delivery process (or the CI/CD process) includes many tools, distributed teams, open-source code, and cloud platforms. Constant focus on speed to release software to market, along with the traditional slow and manual security checks has caused gaps in continuous security as an important piece in the software supply chain. Today organizations feel more susceptible to external and internal cyber threats due to the vast attack surface in their applications supply chain and the lack of end-to-end governance and risk management.
The software team must secure its software delivery process to avoid vulnerability and security breaches. This needs to be achieved with existing tool chains and without extensive rework of the delivery processes. This talk will present strategies and techniques for providing visibility into the true risk of the existing vulnerabilities, preventing the introduction of security issues in the software, resolving vulnerabilities in production environments quickly, and capturing the deployment bill of materials (DBOM).
Speakers:
Bob Boule
Robert Boule is a technology enthusiast with PASSION for technology and making things work along with a knack for helping others understand how things work. He comes with around 20 years of solution engineering experience in application security, software continuous delivery, and SaaS platforms. He is known for his dynamic presentations in CI/CD and application security integrated in software delivery lifecycle.
Gopinath Rebala
Gopinath Rebala is the CTO of OpsMx, where he has overall responsibility for the machine learning and data processing architectures for Secure Software Delivery. Gopi also has a strong connection with our customers, leading design and architecture for strategic implementations. Gopi is a frequent speaker and well-known leader in continuous delivery and integrating security into software delivery.
Let's dive deeper into the world of ODC! Ricardo Alves (OutSystems) will join us to tell all about the new Data Fabric. After that, Sezen de Bruijn (OutSystems) will get into the details on how to best design a sturdy architecture within ODC.
Search and Society: Reimagining Information Access for Radical FuturesBhaskar Mitra
The field of Information retrieval (IR) is currently undergoing a transformative shift, at least partly due to the emerging applications of generative AI to information access. In this talk, we will deliberate on the sociotechnical implications of generative AI for information access. We will argue that there is both a critical necessity and an exciting opportunity for the IR community to re-center our research agendas on societal needs while dismantling the artificial separation between the work on fairness, accountability, transparency, and ethics in IR and the rest of IR research. Instead of adopting a reactionary strategy of trying to mitigate potential social harms from emerging technologies, the community should aim to proactively set the research agenda for the kinds of systems we should build inspired by diverse explicitly stated sociotechnical imaginaries. The sociotechnical imaginaries that underpin the design and development of information access technologies needs to be explicitly articulated, and we need to develop theories of change in context of these diverse perspectives. Our guiding future imaginaries must be informed by other academic fields, such as democratic theory and critical theory, and should be co-developed with social science scholars, legal scholars, civil rights and social justice activists, and artists, among others.
Software Delivery At the Speed of AI: Inflectra Invests In AI-Powered QualityInflectra
In this insightful webinar, Inflectra explores how artificial intelligence (AI) is transforming software development and testing. Discover how AI-powered tools are revolutionizing every stage of the software development lifecycle (SDLC), from design and prototyping to testing, deployment, and monitoring.
Learn about:
• The Future of Testing: How AI is shifting testing towards verification, analysis, and higher-level skills, while reducing repetitive tasks.
• Test Automation: How AI-powered test case generation, optimization, and self-healing tests are making testing more efficient and effective.
• Visual Testing: Explore the emerging capabilities of AI in visual testing and how it's set to revolutionize UI verification.
• Inflectra's AI Solutions: See demonstrations of Inflectra's cutting-edge AI tools like the ChatGPT plugin and Azure Open AI platform, designed to streamline your testing process.
Whether you're a developer, tester, or QA professional, this webinar will give you valuable insights into how AI is shaping the future of software delivery.
GraphRAG is All You need? LLM & Knowledge GraphGuy Korland
Guy Korland, CEO and Co-founder of FalkorDB, will review two articles on the integration of language models with knowledge graphs.
1. Unifying Large Language Models and Knowledge Graphs: A Roadmap.
https://arxiv.org/abs/2306.08302
2. Microsoft Research's GraphRAG paper and a review paper on various uses of knowledge graphs:
https://www.microsoft.com/en-us/research/blog/graphrag-unlocking-llm-discovery-on-narrative-private-data/
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Paper presented at SYNERGY workshop at AVI 2024, Genoa, Italy. 3rd June 2024
https://alandix.com/academic/papers/synergy2024-epistemic/
As machine learning integrates deeper into human-computer interactions, the concept of epistemic interaction emerges, aiming to refine these interactions to enhance system adaptability. This approach encourages minor, intentional adjustments in user behaviour to enrich the data available for system learning. This paper introduces epistemic interaction within the context of human-system communication, illustrating how deliberate interaction design can improve system understanding and adaptation. Through concrete examples, we demonstrate the potential of epistemic interaction to significantly advance human-computer interaction by leveraging intuitive human communication strategies to inform system design and functionality, offering a novel pathway for enriching user-system engagements.
State of ICS and IoT Cyber Threat Landscape Report 2024 previewPrayukth K V
The IoT and OT threat landscape report has been prepared by the Threat Research Team at Sectrio using data from Sectrio, cyber threat intelligence farming facilities spread across over 85 cities around the world. In addition, Sectrio also runs AI-based advanced threat and payload engagement facilities that serve as sinks to attract and engage sophisticated threat actors, and newer malware including new variants and latent threats that are at an earlier stage of development.
The latest edition of the OT/ICS and IoT security Threat Landscape Report 2024 also covers:
State of global ICS asset and network exposure
Sectoral targets and attacks as well as the cost of ransom
Global APT activity, AI usage, actor and tactic profiles, and implications
Rise in volumes of AI-powered cyberattacks
Major cyber events in 2024
Malware and malicious payload trends
Cyberattack types and targets
Vulnerability exploit attempts on CVEs
Attacks on counties – USA
Expansion of bot farms – how, where, and why
In-depth analysis of the cyber threat landscape across North America, South America, Europe, APAC, and the Middle East
Why are attacks on smart factories rising?
Cyber risk predictions
Axis of attacks – Europe
Systemic attacks in the Middle East
Download the full report from here:
https://sectrio.com/resources/ot-threat-landscape-reports/sectrio-releases-ot-ics-and-iot-security-threat-landscape-report-2024/
State of ICS and IoT Cyber Threat Landscape Report 2024 preview
Applications of genetic engineering techniques in agriculture byB. DEVADATHA
1. Applications Of Genetic Engineering
Techniques In Agriculture
B.DEVADATHA
123680029
m.Sc biochemistry and
2. Why genetically engineer plants?
• To improve the agricultural, horticultural or
ornamental value of a crop plant
• To serve as a bioreactor for the production of
economically important proteins or metabolites
• To provide a powerful means for studying the
action of genes (and gene products) during
development and other biological processes
3. Genetic engineering techniques applied to plants
METHOD SALIENT FEATURES
1.VECTOR MEDIATED GENE
TRANSFER
a. Agrobacterium mediated gene
transfer
b. Plant viral vectors
Very efficient but limited to a selected group of plants
Ineffective, hence not widely used
2.DIRECT OR VECTORLESS
DNA TRANSFER
a. Electroporation
b. Microprojectile
c. Liposome fusion
d. Silicon carbide fibres
Mostly confined to protoplasts that can be regenerated to viable
plants
Limited use only one cell can be microinjected at a time
Confined to protoplasts that can be regenerated into viable whole
plants
Requires regenerable cell suspensions
3 CHEMICAL METHODS
a. Polyethylene glycol mediated
b.Diethylaminoethyl(DEAE)dextra
n- mediated
Confined to protoplasts. Regeneration of fertile plants is frequently
problematical
Does not result in stable transformants
4. AGROBACTERIUM MEDIATED GENE TRANSFER
• Agrobacterium tumefaciens is a naturally occurring
Gram negative bacteria
• Causes Crown gall disease by transferring T-DNA from
its Ti plasmid in variety of dicotyledonous plants
• Plants infected with this bacterium develop tumour like
swellings galls that typically occur at crown of plant just
above soil level
• Following infection ,the bacterium transfers part of its
DNA into plant & this DNA integrates into plant genome
causing production of tumors and associated changes
in plant metabolism
5. Process of Infection
• A.tumefaciens infects plants through wounds
• The motile bacterial cells are attracted to
wound site by phenolic compounds such as
acetosyringone secreted at wound site
through specific chemotactic receptors
present in bacterial membrane
• Acetosyringone at higher concentrations
activates Vir genes leading to production of
proteins that are inserted into bacterial cell
membrane for upake of opines produced by
tumors
• Acetosyringone also causes production of an
Endonuclease that encodes a part of Ti
plasmid termed T-DNA
6. Gene gun/Biolistic bombardment system
Desired DNA is coated on microscopic gold or tungsten beads
The particles are fired by a gun into plant tissue and penetrate the cell wall
DNA unwinds from gold carrier
particle and enters the nucleus
7. Applications of Plant Genetic Engineering
• Improved Nutritional Quality
• Insect resistance
• Disease resistance
• Herbicide resistance
• Salt tolerance
• Delayed Fruit Ripening
• Biopharmaceuticals and Vaccines
8. Improved Nutritional Quality
• 124 million children worldwide are deficient in vitamin A, which leads to death
and blindness
• b-carotene is precursor to vitamin A & consuming milled rice leads to vitamin A
deficiency
• Mammals make vitamin A from b-carotene,
a common carotenoid pigment normally found
in plant photosynthetic membranes
Daffodil phytoene synthase gene
carotene desaturase gene
Daffodil lycopene b-cyclase gene
Endogenous human gene
9. RAINBOW CAULIFLOWER
• Produced by traditional breeding –Non
Transgenic
• The Orange cauliflower has higher
than normal levels of b-carotene
that encourages healthy skin
• Purple colour comes from
Anthocyanin which may prevent
Heart disease by slowing blood clotting
• Tests of the orange cauliflowers in
America found that they contained 25
times the concentrations of beta
carotene in normal cauliflowers
10. Insect resistance
• It is estimated about 15% of world’s crop yield is lost
through insects
• Bacillus thuringiensis is a Gram negative ,soil bacterium
produces parasporal crystalline proteinous toxin with
insecticidal activity
• When the Bt toxin gene was introduced into economically
imp crop plants they develop resistanace for major insects
obviating the need for use of insecticides
• Bt toxin opens cation selective pores in membranes
,leadng to inflow of the cations into cells that causes
osmotic lysis & destruction of epithelial cells
• Bt genes could be expressed
• in all parts of plants
11. Disease resistance plants
• Genes that provide resistance against plant
viruses have been successfully introduced
into crop plants such as tobacco, tomato,
rice, potato ,etc
• Transgenic tobacco plant
• Expressing tobacco mosaic virus coat protein
gene were first developed
• Viral capsids inhibit viral replication of TMV when infected
• Virus coat protein mediated protection is
successful for viruses with ss RNA
12. Herbicide resistance
• Genes for resistance against certain Herbicides have
been introduced into crop plants so they can thrive even
when exposed to Herbicides
• Glyphosate is a non selective Herbicide that inhibits 5-
enolpyruvylshikimate-3-phosphate synthase (EPSP) a
chloroplast enzyme in the shikimate pathway and plays a
key role in the synthesis of aromatic amino acids such as
tyrosine and phenylalanine
• Herbicides Sulfonylureas & Imidazolines inhibits
Acetolacetate (ALS),key enzyme in synthesis of Isoleucine
,Leucine & Valine
• Some other Herbicide resistant plants also developed
eg:bromoxynil,atrazine ,phenocarboxylic acids
13. Salt tolerance
• A large fraction of world’s
irrigated land cannot be
used to grow most
important crops due to
increased salinity in soil
• Resarcher’s have created
transgenic tomatoes that
grew well in saline soils
• The transgene introduced
was sodium/proton
antiport pump that
sequestered excess
sodium in vacuole of leaf
cells
14. Flavr savr tomato
• Most tomatoes that have to be shipped to
market are harvested before they are ripe
otherwise, Ethylene synthesized by tomato causes
them to ripen & spoil before they reach customer
• Flavr savr tomatoes have been constructed that
Express an antisense mRNA complementary to
mRNA for an enzyme involved in ethylene
Production
These tomatoes make only 10% of normal amount
of enzyme thus delaying ethylene production
15. Biopharmaceuticals and Vaccines
• Expression of Hepatitis B Surface
Antigen in Transgenic plants
• Tobacco plants were genetically
transformed with gene encoding
Hepatitis B surface antigen
(HBsAg) linked to a nominally
constitutive promoter were
generated
• Recombinant HBsAg purified
from transgenic plant had
properties similar to Human
serum derived HBsAg
16. Edible vaccines
• Vaccines consisting of transgenic plant-derived antigens offer
a new strategy for development of safe, inexpensive vaccines.
• The vaccine antigens can be eaten with the edible part of the
plant or purified from plant material
Rabies- Tomato plants expressing rabies antigens could induce
antibodies in mice
Cholera-Transgenic potato with CT-B gene of Vibrio
cholerae was shown to be efficacious in mice .
Norwalk virus- transgenic potato expressing norwalk virus
antigen showed seroconversion
Hepatitis B- First human trials of a potato-based vaccine
against hepatitis B have reported encouraging results
If vaccines are intimately presented together with food, the
guts immune system faces a conundrum
17. What are some of the advantages of GM
foods?
GM crops are more productive and have a larger yield.
Offer more nutritional value and better flavor.
A possibility that they could eliminate allergy-causing properties in some
foods.
Inbuilt resistance to pests, weeds and disease.
More capable of thriving in regions with poor soil or adverse climates.
More environment friendly as they require less herbicides and
pesticides.
Foods are more resistant and stay ripe for longer so they can be shipped
long distances or kept on shop shelves for longer periods.
As more GM crops can be grown on relatively small parcels of land, GM
crops are an answer to feeding growing world populations.
18. What are some of the advantages of GM foods?
• Disease resistance There are many viruses, fungi
and bacteria that cause plant diseases.
• Plant biologists are working to create plants
with genetically-engineered resistance to these
diseases.
• Cold tolerance Unexpected frost can destroy
sensitive seedlings. An antifreeze gene from cold
water fish has been introduced into plants such
as tobacco and potato.
• With this antifreeze gene, these plants are able
to tolerate cold temperatures that normally grow
19. Environmental & health impacts of GM
crops
• Toxic to harmless non-target species. Long-term
exposure to pollen from GM insect resistant maize
causes adverse effects on the behaviorand survival
of the monarch butterfly
• Toxic to beneficial insects. GM Bt crops adversely
affectbeneficial insects important to controlling
maize pests, such as green lacewings
• A threat to soil ecosystems. Many Bt crops
secrete their toxin from their roots into the soil.
Residues left in the field contain the active Bt toxin
• Risk for aquatic life. Leaves or grain from Bt maize
can enter water courses where the toxin can
accumulate in organismsand possibly exert a toxic
effect
20. Environmental & health impacts of GM
crops
• Herbicide-tolerant crops may cross-pollinate
weeds, resulting in "superweeds"
• Certain gene products may be allergens, thus
causing harm to human health
• recent study found the Cry1Ab Bt toxin in the
blood of pregnant women and their fetuses
showing that it can cross the placental
boundary
• Studies indicate Roundup may be toxic to
mammals and could interfere with hormones
• There may be unintended harm to wildlife and
beneficial insects
21. conclusion
• Genetically-modified foods have the potential to solve many of the
world's hunger and malnutrition problems, and to help protect and preserve the
environment by increasing yield and reducing reliance upon chemical pesticides
and herbicides.
• Yet there are many challenges ahead for governments, especially in the areas of
safety testing, regulation, international policy and food labeling.
• Many people feel that genetic engineering is the inevitable wave of the future
and that we cannot afford to ignore a technology that has such enormous
potential benefits.
• However, we must proceed with caution to avoid causing unintended harm to
human health and the environment as a result of our enthusiasm for this
powerful technology.
• At the present time, genetically modified foods are dangerous for the world
that we live in, not just for its people, but the delicate balance between
organisms that inhabit it.
22. Sources
• GM Science Review First Report (http:/ / www. bis. gov. uk/ files/
file15655. pdf), Prepared by the UK GM Science Review panel
(July2003).
• Maxmen, Amy (2 May 2012) First plant-made drug on the market (http:/
/ blogs. nature. com/ news/ 2012/ 05/first-plant-made-drug-on-the-
market. html) Nature, Biology & Biotechnology, Industry. Retrieved 26
June 2012
• Conner AJ, Glare TR, Nap JP. The release of genetically modified crops
into the environment. Part II. Overview of ecological risk assessment
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