This document provides an overview of glycolysis and gluconeogenesis. It discusses the key reactions and enzymes involved in glycolysis, which converts glucose to pyruvate, producing a small amount of ATP. Three reactions of glycolysis are irreversible. Under anaerobic conditions, pyruvate can be reduced to lactate. Glycolysis occurs in the cytosol of almost every living cell and was the first metabolic pathway to be studied in detail. Phosphorylation of intermediates traps molecules in the cell and provides energy for chemical reactions. The document also compares the enzymes hexokinase and glucokinase, and examines regulatory points in glycolysis.
Carbohydrate metabolism involves the different biochemical processes responsible for the formation, breakdown, and interconversion of carbohydrates in living organisms.
Glycogen is the storage form of Glucose which maintain the blood glucose level under various condition. Glycogen Metabolism is the important pathway of carbohydrate metabolism which gives the information about the glycogen synthesis (Glycogenesis), Glycogen breakdown (Glucogenolysis). Glycogen metabolism also gives the information how this pathway is regulated. Their are various diseases which are associated with this metabolism, commonly known as Glycogen storage diseases.
It is an metabolic pathway of synthesis of glucose from non carbohydrate precursors like pyruvate, lactate, amino acid, glycerol etc. Main sites are liver and kidney. It uses enzymes from both cytosol and mitochondria.
Complete Set of Metabolism of Carbohydrate in that second chapter, glycolysis.
This presentation covers complete glycolysis pathway with step wise animated reactions and it includes clinical aspects also. This presentation is good for MBBS students.
A detailed account of process of gluconeogenesis with mechanisms of important enzymes.We shall also talk extensively about why the process is not the reversible o glycolysis as is commonly perceived. Also focused on its regulatory aspect in conjunction with glycolysis.
Gluconeogenesis- Steps, Regulation and clinical significanceNamrata Chhabra
Gluconeogenesis- Thermodynamic barriers, substrates of gluconeogenesis, reciprocal regulation of glycolysis and gluconeogenesis, biological and clinical significance
The glucuronic acid pathway is a quantitatively minor route of glucose metabolism. Like the pentose phosphate pathway, it provides biosynthetic precursors and inter-converts some less common sugars to ones that can be metabolized.
Carbohydrate metabolism involves the different biochemical processes responsible for the formation, breakdown, and interconversion of carbohydrates in living organisms.
Glycogen is the storage form of Glucose which maintain the blood glucose level under various condition. Glycogen Metabolism is the important pathway of carbohydrate metabolism which gives the information about the glycogen synthesis (Glycogenesis), Glycogen breakdown (Glucogenolysis). Glycogen metabolism also gives the information how this pathway is regulated. Their are various diseases which are associated with this metabolism, commonly known as Glycogen storage diseases.
It is an metabolic pathway of synthesis of glucose from non carbohydrate precursors like pyruvate, lactate, amino acid, glycerol etc. Main sites are liver and kidney. It uses enzymes from both cytosol and mitochondria.
Complete Set of Metabolism of Carbohydrate in that second chapter, glycolysis.
This presentation covers complete glycolysis pathway with step wise animated reactions and it includes clinical aspects also. This presentation is good for MBBS students.
A detailed account of process of gluconeogenesis with mechanisms of important enzymes.We shall also talk extensively about why the process is not the reversible o glycolysis as is commonly perceived. Also focused on its regulatory aspect in conjunction with glycolysis.
Gluconeogenesis- Steps, Regulation and clinical significanceNamrata Chhabra
Gluconeogenesis- Thermodynamic barriers, substrates of gluconeogenesis, reciprocal regulation of glycolysis and gluconeogenesis, biological and clinical significance
The glucuronic acid pathway is a quantitatively minor route of glucose metabolism. Like the pentose phosphate pathway, it provides biosynthetic precursors and inter-converts some less common sugars to ones that can be metabolized.
For More Medicine Free PPT - http://playnever.blogspot.com/
For Health benefits and medicine videos Subscribe youtube channel - https://www.youtube.com/playlist?list=PLKg-H-sMh9G01zEg4YpndngXODW2bq92w
Carbohydrates are the sugars, starches and fibers found in fruits, grains, vegetables and milk products. Though often maligned in trendy diets, carbohydrates — one of the basic food groups — are important to a healthy diet.
Gluconeogenesis: Defined as biosynthesis of glucose from non-carbohydrate precursors
-Gluconeogenesis: an intro
-Thermodynamic Barriers (Each barrier detail explanation)
- Energetics of gluconeogenesis
-Substrates of gluconeogenesis (each substrate and pathway explained)
-Regulation of Gluconeogenesis, hormonal and transcriptional regulation
To understand how the glycolytic pathway is converts glucose to pyruvate.
To understand conservation of chemical potential energy in the form of ATP and NADH.
To learn the intermediates, enzyme, and cofactors of the glycolytic pathway.
Operation “Blue Star” is the only event in the history of Independent India where the state went into war with its own people. Even after about 40 years it is not clear if it was culmination of states anger over people of the region, a political game of power or start of dictatorial chapter in the democratic setup.
The people of Punjab felt alienated from main stream due to denial of their just demands during a long democratic struggle since independence. As it happen all over the word, it led to militant struggle with great loss of lives of military, police and civilian personnel. Killing of Indira Gandhi and massacre of innocent Sikhs in Delhi and other India cities was also associated with this movement.
Model Attribute Check Company Auto PropertyCeline George
In Odoo, the multi-company feature allows you to manage multiple companies within a single Odoo database instance. Each company can have its own configurations while still sharing common resources such as products, customers, and suppliers.
Unit 8 - Information and Communication Technology (Paper I).pdfThiyagu K
This slides describes the basic concepts of ICT, basics of Email, Emerging Technology and Digital Initiatives in Education. This presentations aligns with the UGC Paper I syllabus.
June 3, 2024 Anti-Semitism Letter Sent to MIT President Kornbluth and MIT Cor...Levi Shapiro
Letter from the Congress of the United States regarding Anti-Semitism sent June 3rd to MIT President Sally Kornbluth, MIT Corp Chair, Mark Gorenberg
Dear Dr. Kornbluth and Mr. Gorenberg,
The US House of Representatives is deeply concerned by ongoing and pervasive acts of antisemitic
harassment and intimidation at the Massachusetts Institute of Technology (MIT). Failing to act decisively to ensure a safe learning environment for all students would be a grave dereliction of your responsibilities as President of MIT and Chair of the MIT Corporation.
This Congress will not stand idly by and allow an environment hostile to Jewish students to persist. The House believes that your institution is in violation of Title VI of the Civil Rights Act, and the inability or
unwillingness to rectify this violation through action requires accountability.
Postsecondary education is a unique opportunity for students to learn and have their ideas and beliefs challenged. However, universities receiving hundreds of millions of federal funds annually have denied
students that opportunity and have been hijacked to become venues for the promotion of terrorism, antisemitic harassment and intimidation, unlawful encampments, and in some cases, assaults and riots.
The House of Representatives will not countenance the use of federal funds to indoctrinate students into hateful, antisemitic, anti-American supporters of terrorism. Investigations into campus antisemitism by the Committee on Education and the Workforce and the Committee on Ways and Means have been expanded into a Congress-wide probe across all relevant jurisdictions to address this national crisis. The undersigned Committees will conduct oversight into the use of federal funds at MIT and its learning environment under authorities granted to each Committee.
• The Committee on Education and the Workforce has been investigating your institution since December 7, 2023. The Committee has broad jurisdiction over postsecondary education, including its compliance with Title VI of the Civil Rights Act, campus safety concerns over disruptions to the learning environment, and the awarding of federal student aid under the Higher Education Act.
• The Committee on Oversight and Accountability is investigating the sources of funding and other support flowing to groups espousing pro-Hamas propaganda and engaged in antisemitic harassment and intimidation of students. The Committee on Oversight and Accountability is the principal oversight committee of the US House of Representatives and has broad authority to investigate “any matter” at “any time” under House Rule X.
• The Committee on Ways and Means has been investigating several universities since November 15, 2023, when the Committee held a hearing entitled From Ivory Towers to Dark Corners: Investigating the Nexus Between Antisemitism, Tax-Exempt Universities, and Terror Financing. The Committee followed the hearing with letters to those institutions on January 10, 202
A Strategic Approach: GenAI in EducationPeter Windle
Artificial Intelligence (AI) technologies such as Generative AI, Image Generators and Large Language Models have had a dramatic impact on teaching, learning and assessment over the past 18 months. The most immediate threat AI posed was to Academic Integrity with Higher Education Institutes (HEIs) focusing their efforts on combating the use of GenAI in assessment. Guidelines were developed for staff and students, policies put in place too. Innovative educators have forged paths in the use of Generative AI for teaching, learning and assessments leading to pockets of transformation springing up across HEIs, often with little or no top-down guidance, support or direction.
This Gasta posits a strategic approach to integrating AI into HEIs to prepare staff, students and the curriculum for an evolving world and workplace. We will highlight the advantages of working with these technologies beyond the realm of teaching, learning and assessment by considering prompt engineering skills, industry impact, curriculum changes, and the need for staff upskilling. In contrast, not engaging strategically with Generative AI poses risks, including falling behind peers, missed opportunities and failing to ensure our graduates remain employable. The rapid evolution of AI technologies necessitates a proactive and strategic approach if we are to remain relevant.
The French Revolution, which began in 1789, was a period of radical social and political upheaval in France. It marked the decline of absolute monarchies, the rise of secular and democratic republics, and the eventual rise of Napoleon Bonaparte. This revolutionary period is crucial in understanding the transition from feudalism to modernity in Europe.
For more information, visit-www.vavaclasses.com
2024.06.01 Introducing a competency framework for languag learning materials ...Sandy Millin
http://sandymillin.wordpress.com/iateflwebinar2024
Published classroom materials form the basis of syllabuses, drive teacher professional development, and have a potentially huge influence on learners, teachers and education systems. All teachers also create their own materials, whether a few sentences on a blackboard, a highly-structured fully-realised online course, or anything in between. Despite this, the knowledge and skills needed to create effective language learning materials are rarely part of teacher training, and are mostly learnt by trial and error.
Knowledge and skills frameworks, generally called competency frameworks, for ELT teachers, trainers and managers have existed for a few years now. However, until I created one for my MA dissertation, there wasn’t one drawing together what we need to know and do to be able to effectively produce language learning materials.
This webinar will introduce you to my framework, highlighting the key competencies I identified from my research. It will also show how anybody involved in language teaching (any language, not just English!), teacher training, managing schools or developing language learning materials can benefit from using the framework.
5. GLYCOLYSIS
Glycolysis occurs in almost every living cell.
It occurs in cytosol.
It was the first metabolic sequence to be studied.
Most of the work done in 1930s by the German biochemist G.
Embden Meyerhof Warburg.
That is why it is also called Embden-Meyerhof pathway.
It is a Greek word.
Glykos sweet
Lysis loosing
Glycolysis loosing or splitting of glucose
6.
7. Glycolysis
Glc is an important fuel for most organisms.
Why is Glc chosen?
Glc is one of the monosaccarides formed
formaldehyde under prebiotic conditions.
Glc has a low tendency to glycosylate proteins
Fermentations provide usable energy in the absence of
oxygen
Why is a relatively inefficient metabolic pathway so
extensively used?
Answer: It does not require oxygen
Obligate anaerobes
Facultative anaerobes
8.
9.
10. Glycolysis
• 3 of the reactions of glycolysis are irreversible.
• Pyruvate is the end product of glycolysis in tissues with
mitochondria.
• This series of 10 reactions, called aerobic glycolysis, sets the
stage for the oxidative decarboxylation of pyruvate to Acetyl
CoA, major fuel of the citric acid cycle.
• Under anaerobic conditions, pyruvate can be reduced by
NADH to form lactate.
• This happens when the rate of NADH formation exceeds the
oxidative capacity of the cell.
Glucose Lactate (anaerobic glycolysis)
• If there is no mitochondria (RBCs) or cells that have
insufficient oxygen.
11. Stages of glycolysis
Stage I
– Glc is converted to fructose 1,6 bisphosphate by
• Phosphorylation
• Isomerization
• Second phosphorylation
Goal: To trap the Glc in the cell
12.
13. IMPORTANCE OF PHOSPHARYLATED INTERMEDIATES
Each of the 9 glycolytic intermediates between glucose
and pyruvate is phosphorylated. The phosphate group has
3 functions.
1. Because the P group is ionized at pH 7 and has charge and
because plasma membranes are not permeable to the charged
mols, the phosphorylated molecules can not pass the membranes.
They are trapped in the cell.
2. P groups are necessary for chemical energy. Energy is released
after the hydrolysis of ATP.
3. Binding of P groups to the active sites of enzymes provides binding
energy which decreases the activation energy and increase the
specificity of enzyme-catalyzed reactions.
14. PHOSPHORYLATION OF GLUCOSE
Glucose obtained from the diet through intestinal hydrolysis of
lactose, sucrose, glycogen, or starch is brought into the hexose
phosphate pool through the action of hexokinase.
– Free glucose phosphorylated by hexokinase (irreversible reaction 1)
Hexokinase actually traps glucose in a form that does not diffuse
out of the cell. Because phosphorylated sugar molecules do not
readily penetrate cell membranes without specific carriers, this
commits glucose to further metabolism in the cell. In all tissues, the
phosphorylation of glucose is catalyzed by hexokinase, one of the
three regulatory enzymes of glycolysis.
20. Difference between hexokinase and glucokinase
Hexokinase:
– Its function is to make sure there is enough glc for the
tissues, even in the presence of low blood glc
concentrations, by phosphorylating all the glc concentration
gradient between the blood and the intracellular
environment.
Glucokinase:
– Its function is to remove glc from the blood following a meal.
Hexokinase, phosphofructokinase and pyruvate
kinase are 3 regulatory enzymes of glycolysis.
21. Hexokinase vs glucokinase
Liver has an additional enzyme, glucokinase, that phosphorylate
only glc
1. Glucokinase has a high Km, because it phosphorylates glc only
when its concentration is high. This occurs during the brief
period after consumption of a carbohydrate rich meal, when
high glc are delivered by portal vein.
2. Glucokinase has a high Vmax, allowing the liver to remove
effectively this flood of glc from the portal blood. So this
prevents extreme hyperglycemia after meals.
22. SUMMARY
Hexokinase Glucokinase
Tissue dist all liver
Km low high
Vmax low high
Substrate D-glc and other D-glc only
Inhibition by G-6-P Yes no
23. More about HK
Hexokinase, like adenylate kinase and all other
kinases, requires Mg (or Mn) for activity.
Hexokinase is also one of the induced-fit model
enzymes.
– It has two lobes that move towards each other when Glc is
bound!
Substrate-induced cleft closing is a general feature of
kinases.
Other kinases (Pyruvate kinase, phosphoglycerate
kinase and PFK) also contain clefts between lobes that
close when substrate is bound.
24. 2. ISOMERIZATION OF G-6-P
The isomerization of G-6-P (an aldose sugar) to
F-6-P (a ketose sugar) is catalyzed by
pgosphoglucose isomerase. The reaction is
readily reversible, is NOT a rate limiting or
regulated step.
25.
26. 3. PHOSPHORYLATION OF F-6-P
Irreversible phosphorylation reaction catalyzed by
PFK (phosphofructokinase) is the most important
control point of glycolysis.
Within the cell, the PFK reaction is the rate-limiting
step in the glycolytic breakdown of glc. It is
controlled by the concentrations of the substrates
ATP and F-6-P
29. 4. CLEAVAGE OF F-1,6BIP
Aldolase cleaves F-1,6-biP to
– dihydroxyacetonephosphate
– glyceraldehyde 3-P.
The reaction is reversible is not subject
to regulation.
30.
31. 5. ISOMERIZATION OF DIHYDROXYACETONE-P
Triose phosphate isomerase (TIM) catalyzes the
reversible interconversion of dihydroxyacetone
phosphate and glyceraldehyde 3-phosphate.
X-ray crystallographic and other studies showed
that Glu 165 plays the role of a general acid-base
catalyst.
TIM has 8 parallel beta and 8 alpha helices (
barrel). This structure is also found in
• Aldolase
• Enolase
• Pyruvate kinase
32.
33.
34. more
Di-OH-acetone-P can not proceed further on the
direct pathway of glycolysis and must be
isomerized to glyceraldehyde-3-P for further
metabolism in the glycolytic pathway.
So, this isomerization results 2 mols of
glyceraldehyde -3-P from fructose 1,6 biP.
35.
36. 6. OXIDATION OF GLYCERALDEHYDE 3-P.
Because there is only a limited amount of NAD+ in
the cell, the NADH formed in this reaction must be
reoxidized back to NAD+ for glycolysis to continue.
Two major mechanisms for oxidizing NADH are
– The NADH-linked conversion of pyruvate to lactate
– Oxidation by the respiratory chain.
The high energy P group at carbon of 1,3-bisPG
conserves much of the free energy produced by the
oxidation of Glycerate-3-P
46. Free energy profiles for glyceraldehyde oxidation followed by
Acyl-phosphate formation: A) Hypothetical B)Actual
47.
48.
49. 7: FORMATION OF ATP FROM 1,3-BisPGLYCERATE AND ADP
This step is a substrate-level phosphorylation in which the
production of a high-energy P is coupled to the conversion of
substrate to product, instead of resulting from oxidative
phosphorylation. The energy trapped in this new high-energy P
will be used to make ATP in the next reaction of glycolysis. The
formation of ATP by P group transfer from a substrate such as
1,3-bisphosphoglycerate is referred to as a substrate-level
phosphorylation. Unlike most other kinases, this reaction is
reversible.
2 mols 1,3biPGlycerate 2ATP replaces the 2ATP consumed
earlier with the formation of G-6-P and fructose 1,6bisP.
50.
51. More reactions
8. SHIFT OF THE P FROM CARBON 3 TO CARBON 2
9. DEHYDRATION OF 2-PHOSPHOGLYCERATE
– Pyruvate first appears in its enol form.
10. FORMATION OF PYRUVATE
– Another substrate-level phosphorylation.
56. Question: Why does PEP have such a
high P-ryl potential?
Answer: The P-ryl group traps the molecule in its
unstable enol form. When P-ryl is given to ADP,
the enol undergoes a conversion into the more
stable ketone, namely pyruvate.
Thus, the high P-ryl transfer potential of PEP
comes from the large driving force of the
subsequent enol ketone conversion.
57. Pyruvate kinase deficiency:
It leads to hemolytic anemia (RBC destruction).
The normal mature RBC has no mitochondria, thus it completely
depends on glycolysis to produce ATP.
Patients RBCs typically have 5% to 25% of normal pyruvate kinase
activity, and therefore the rate of glycolysis in these cells is decreased.
The anemia observed in PK-deficiency may be a consequence of the
reduced rate of glycolysis.
The rate of ATP synthesis is thought to be inadequate to meet the
energy needs of the cell and to maintain the structural integrity of the
RBC.
The inability of RBC to maintain its membrane changes in the shape,
phagocytosis by the cell takes place.
The premature death and lysis of RBC causes hemolytic anemia.
58. Maintaining redox balances-1
The conversion of Glc 2 mols of Pyruvate and net
synthesis of ATP
An energy-converting pathway that stopped at pyruvate
would not proceed for long, because redox balance has
not been maintained!
GAP dehydrogenase uses NAD+ and there are limited
amounts of NAD+ in the cell.
Therefore, NAD+ must be regenerated for glycolysis to
proceed!
Thus, the final pathway in glycolysis is to generate
NAD+ through pyruvate metabolism.