Radioimmunoassay is a binding assay that uses antibodies and radioactivity to measure the amount of bound and free antigen. Radioactively labeled antigen, called a tracer, competes with unlabeled antigen for binding sites on antibodies. The ratio of bound to free tracer is measured and used to determine the amount of unlabeled antigen present. Radioimmunoassay is versatile, fast, and sensitive but requires the use of radioactivity which is hazardous. It works on the principle that the distribution of antigen between bound and free fractions is determined by the association constant of the antibody-antigen interaction.