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Enzyme
Classification
Classification of Enzymes
According to the International
Union of Biochemistry or
Biochemists Enzymes are
classified into SIX major
classes
Class I – Oxidoreductases,
catalyze oxidation reductions
Class II – Transferases
Catalyzing group transfer
Class III– Hydrolases
Catalyzing hydrolysis
Class IV– Lyases
Catalyzing addition of groups to
═ bonds
Class V -- Isomerases
Catalyzing isomerization
Class VI – Ligases
Catalyzing condensations
I Oxidoreductases
They catalyze oxidation
and reduction reaction.
These enzymes are
divided into three groups.
(a) Oxidases: Those which use
oxygen as hydrogen as acceptor
eg:- L amino acid oxidases.
L a.a + H2O + O2  2 oxoacids +
NH3+H2O2
Eg: D-amino acid oxidases,
Tyrosinase,Cyt-oxidases, Uricases.
(b) Anaerobic Dehydrogenases
Those which use some other
substances as Hydrogen acceptor ,
eg: NAD,NADP
Enz: 1) Malate Dehydrogenase
2) Succinate Dehydrogenase
3) Lactate Dehydrogenase.
These anaerobic dehydrogenases can
further be sub-classified as
(i) Those acting on CH-OH of donors
eg: Glycero-P-dehydrogenase
Gly-3-P + NAD ═ Di Hydroxy Acetone P
+ NADH
Other eg:-- lactate Dehydrogenase
Malate Dehydrogenase
Isocitrate Dehydrogenase
(ii)Those acting on CH-CH group of donors
eg: Dihydrouracil dehydrogenase
4,5 dihydrouracil + NAD+
═ Uracil + NADH
(iii) Those acting on CH-NH2 group of donor
with NAD or NADP as acceptor.
eg: Glutamate Dehydrogenase
Glutamate + H2O + NAD+
═ 2 oxoglutarate
NH3 + NADH
(C) Hydroperoxidases
Acting on H2O2 as acceptor, or those which
use hydrogen peroxide as substrate.
eg:Catalase , Peroxidase
H2O2 + H2O2 ══ O2 +2H2O
II Transferases
 They catalyze the transfer of some groups from
one molecule to the other.
(1) Methyl Transfer: Methyl transferases
a. S-adenosyl Methionine + Guanidino acetate
║Guanidino acetate Methyl transferase
S-adenosyl homocysteine + Creatine
b. Serine hydroxy methyl transferase
Serine  Glycine
(2)Carboxl transfer-- eg: Aspartate
Transcarbamylase
(3) Aldehyde, Keto Residue Transfer--eg:
Transaldolase, Transketolase
(4) Glucosyl Transfer--eg: Transglucosylase
(5)Amino Grp Transfer--eg: Transaminases
(6) Phosphorus Transfer eg:
Glucokinases,Hexokinases
(7) Transfering Sulphur-- eg: Trans-sulfurase
(8) Acyl Transferases—eg:Transacylases
III Hydrolases
They catalyze the
hydrolysis of substrate
by addition of water
molecule across the
bond which they split
(I) Acting on Ester bonds-Esterases
• (a) Acetylcholine-esterase
Acetylcholine +H2O  Choline + Acetic Acid
• (b) Alkaline Phosphatase
Orthophosphoric mono ester+H2O  an Alcohol
+H3PO4
Eg: G-6-P + H2O D glucose + H3PO4
(ii) Acting on Glycosyl compound
• Amylases β galactosidases
(iii) Acting on Peptide bonds
• Peptidases 
aminopeptidases,carboxypeptidases
(iv)Acting on C-N bonds
• Deamidases Urease (Urea Amido Hydrolase)
Eg: Urea + H2O = CO2 + 2 NH3
(v) Acting on Acid Anhydride bonds
(a)Pyrophosphate Phosphorylase (Synthetic
Name)
• This enzyme is also called Inorganic pyro
phosphatase (trivial name)
• Pyro PO4 + H2O == 2 ortho phosphate
(b)ATP Phospho Hydrolase (synthetic name)
• ATP ase (trivial name)
• ATP + H2O == ADP + orthophosphate
IV .Lyases
•They catalyze the
splitting of group from
substrate without
hydrolysis or
oxidation,reduction.
(i) C-C Lyases : Pyruvate Decarboxylase
2 oxo acids == an Acid + CO2
(ii) Keto Acid Lyase : Isocitrate Lyase
Citrate + CoA = Acetyl CoA + H2O +oxalo
acetate
(iii) C-oxylyase : Serine Hydrolyase or
Serine Hydratase
Serine + H2O == Pyruvic + NH3 + H2O
Acid
V Isomerases
• They catalyze isomeric
conversions or epimeric
conversions.
• Eg: Isomerases,Racemases,
Epimerases
VI- Ligases
(i) Tyrosine S RNA Ligase or Tyrosyl RNA
Synthetase.
ATP + Tyr. + RNA ═ AMP + Pyr PO4 +
Tyrosyl S RNA
(ii) CTP Synthetase
(iii) GMP Synthetase.
Ligases can join or form
• C-O bonds 
Tyrosyl S. RNA Synthetase
Leucyl S.RNA Synthetase
Alanyl S.RNA Synthetase
• C-N bonds 
GMP Synthetase
CTP Synthetase
• C-C bonds 
(i) Pyruvate CO2 Ligase or Pyruvate
Carboxylase
ATP +Pyruvate +CO2 + H2O = ADP +Oxaloacetate +
Ortho P
(ii) Acetyl CoA CO2 Ligase or Acetyl CoA
Carboxylase
• ATP + Acetyl CoA + CO2 + H2O
║
ADP + Ortho P + Malonyl CoA

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Class of enz lec 4

  • 2. Classification of Enzymes According to the International Union of Biochemistry or Biochemists Enzymes are classified into SIX major classes
  • 3. Class I – Oxidoreductases, catalyze oxidation reductions Class II – Transferases Catalyzing group transfer Class III– Hydrolases Catalyzing hydrolysis Class IV– Lyases Catalyzing addition of groups to ═ bonds Class V -- Isomerases Catalyzing isomerization Class VI – Ligases Catalyzing condensations
  • 4. I Oxidoreductases They catalyze oxidation and reduction reaction. These enzymes are divided into three groups.
  • 5. (a) Oxidases: Those which use oxygen as hydrogen as acceptor eg:- L amino acid oxidases. L a.a + H2O + O2  2 oxoacids + NH3+H2O2 Eg: D-amino acid oxidases, Tyrosinase,Cyt-oxidases, Uricases.
  • 6. (b) Anaerobic Dehydrogenases Those which use some other substances as Hydrogen acceptor , eg: NAD,NADP Enz: 1) Malate Dehydrogenase 2) Succinate Dehydrogenase 3) Lactate Dehydrogenase.
  • 7. These anaerobic dehydrogenases can further be sub-classified as (i) Those acting on CH-OH of donors eg: Glycero-P-dehydrogenase Gly-3-P + NAD ═ Di Hydroxy Acetone P + NADH Other eg:-- lactate Dehydrogenase Malate Dehydrogenase Isocitrate Dehydrogenase
  • 8. (ii)Those acting on CH-CH group of donors eg: Dihydrouracil dehydrogenase 4,5 dihydrouracil + NAD+ ═ Uracil + NADH (iii) Those acting on CH-NH2 group of donor with NAD or NADP as acceptor. eg: Glutamate Dehydrogenase Glutamate + H2O + NAD+ ═ 2 oxoglutarate NH3 + NADH
  • 9. (C) Hydroperoxidases Acting on H2O2 as acceptor, or those which use hydrogen peroxide as substrate. eg:Catalase , Peroxidase H2O2 + H2O2 ══ O2 +2H2O
  • 10. II Transferases  They catalyze the transfer of some groups from one molecule to the other. (1) Methyl Transfer: Methyl transferases a. S-adenosyl Methionine + Guanidino acetate ║Guanidino acetate Methyl transferase S-adenosyl homocysteine + Creatine b. Serine hydroxy methyl transferase Serine  Glycine
  • 11. (2)Carboxl transfer-- eg: Aspartate Transcarbamylase (3) Aldehyde, Keto Residue Transfer--eg: Transaldolase, Transketolase (4) Glucosyl Transfer--eg: Transglucosylase (5)Amino Grp Transfer--eg: Transaminases (6) Phosphorus Transfer eg: Glucokinases,Hexokinases (7) Transfering Sulphur-- eg: Trans-sulfurase (8) Acyl Transferases—eg:Transacylases
  • 12. III Hydrolases They catalyze the hydrolysis of substrate by addition of water molecule across the bond which they split
  • 13. (I) Acting on Ester bonds-Esterases • (a) Acetylcholine-esterase Acetylcholine +H2O  Choline + Acetic Acid • (b) Alkaline Phosphatase Orthophosphoric mono ester+H2O  an Alcohol +H3PO4 Eg: G-6-P + H2O D glucose + H3PO4
  • 14. (ii) Acting on Glycosyl compound • Amylases β galactosidases (iii) Acting on Peptide bonds • Peptidases  aminopeptidases,carboxypeptidases (iv)Acting on C-N bonds • Deamidases Urease (Urea Amido Hydrolase) Eg: Urea + H2O = CO2 + 2 NH3
  • 15. (v) Acting on Acid Anhydride bonds (a)Pyrophosphate Phosphorylase (Synthetic Name) • This enzyme is also called Inorganic pyro phosphatase (trivial name) • Pyro PO4 + H2O == 2 ortho phosphate (b)ATP Phospho Hydrolase (synthetic name) • ATP ase (trivial name) • ATP + H2O == ADP + orthophosphate
  • 16. IV .Lyases •They catalyze the splitting of group from substrate without hydrolysis or oxidation,reduction.
  • 17. (i) C-C Lyases : Pyruvate Decarboxylase 2 oxo acids == an Acid + CO2 (ii) Keto Acid Lyase : Isocitrate Lyase Citrate + CoA = Acetyl CoA + H2O +oxalo acetate (iii) C-oxylyase : Serine Hydrolyase or Serine Hydratase Serine + H2O == Pyruvic + NH3 + H2O Acid
  • 18. V Isomerases • They catalyze isomeric conversions or epimeric conversions. • Eg: Isomerases,Racemases, Epimerases
  • 19. VI- Ligases (i) Tyrosine S RNA Ligase or Tyrosyl RNA Synthetase. ATP + Tyr. + RNA ═ AMP + Pyr PO4 + Tyrosyl S RNA (ii) CTP Synthetase (iii) GMP Synthetase.
  • 20. Ligases can join or form • C-O bonds  Tyrosyl S. RNA Synthetase Leucyl S.RNA Synthetase Alanyl S.RNA Synthetase • C-N bonds  GMP Synthetase CTP Synthetase
  • 21. • C-C bonds  (i) Pyruvate CO2 Ligase or Pyruvate Carboxylase ATP +Pyruvate +CO2 + H2O = ADP +Oxaloacetate + Ortho P (ii) Acetyl CoA CO2 Ligase or Acetyl CoA Carboxylase • ATP + Acetyl CoA + CO2 + H2O ║ ADP + Ortho P + Malonyl CoA