This document provides an overview of protein synthesis and function. It discusses how proteins are produced through transcription and translation. Transcription involves copying DNA into mRNA, which is then translated into a polypeptide chain through the genetic code on ribosomes. The document also covers the structure and function of antibodies, which recognize antigens, and enzymes, which act as catalysts in cells. Key aspects like the production of monoclonal antibodies and how various factors influence enzyme activity are also summarized.
Glycosylation, the attachment of sugar moieties to proteins, is a post-translational modification (PTM) that provides greater proteomic diversity than other PTMs.
Describes the structural organisation of proteins with example and its determination, interrelationship b/w structure and function of proteins, also biologically important peptides is covered.
by Dr. N. Sivaranjani, MD
Glycosylation, the attachment of sugar moieties to proteins, is a post-translational modification (PTM) that provides greater proteomic diversity than other PTMs.
Describes the structural organisation of proteins with example and its determination, interrelationship b/w structure and function of proteins, also biologically important peptides is covered.
by Dr. N. Sivaranjani, MD
Amino acisd structure
Peptide bond formation
Analysis of protein Structure- X-ray Crystallography
Different structural levels of proteins with examples.
Importance of protein structure
Creutzfeldt-Jacob-Disease due to changes in normal protein conformation.
This is based on protein-ligand interaction physical method, which gives us knowledge about how our body protein interacts with other molecule and protein function.
Genomic sequencing a sub-disciplinary branch of genetics and difference between the two sequencers used to sequence the genome basically automated sequencer and fluorescence sequencers and its applications.
Introduction.
Types of Protein – Protein Interaction.
Methods to investigate Protein – Protein Interaction.
Protein – Protein Interaction modulated by Chemical energy.
Two Hybrid Screening.
Overview of Protein – Protein Interaction analysis.
Biological effect of Protein – Protein interaction.
Conclusion.
Reference.
SYNTHETIC PEPTIDE VACCINES AND RECOMBINANT ANTIGEN VACCINED.R. Chandravanshi
What is a Vaccine?
A vaccine is a substance that is introduced into the body to prevent infection or to control disease due to a certain pathogen (a disease-causing organism, such as a virus, bacteria or parasite). The vaccine “teaches” the body how to defend itself against the pathogen by creating an immune response.
1 Unlike traditional pharmaceuticals, vaccines are biologics since they are made from living organisms (biological sources).
2 Specifically, vaccines are preparations of components derived from (or related to) a pathogen; they can typically induce a protective effect through one to three very small doses, in the range of micrograms to milligrams.
3 Immunity lasts for an extended period, from one year up to lifetime protection, including prevention of disease and/or related sequelae.
Synthetic peptide vaccines represent fragments of protein antigen sequences, synthesizing specific B cell and T cell epitopes offer the potential to induce diseases neutralizing immuno response with completely synthetic structure. Now it is well established that short chain peptides can be used to mimic antigenic sites of viruses and thus can be used the basics for vaccines and development. therefore, attempts have been made to synthesize such peptides which act as the serrogate immuunogens, as an alternative to the existing conventional vaccines.
Amino acisd structure
Peptide bond formation
Analysis of protein Structure- X-ray Crystallography
Different structural levels of proteins with examples.
Importance of protein structure
Creutzfeldt-Jacob-Disease due to changes in normal protein conformation.
This is based on protein-ligand interaction physical method, which gives us knowledge about how our body protein interacts with other molecule and protein function.
Genomic sequencing a sub-disciplinary branch of genetics and difference between the two sequencers used to sequence the genome basically automated sequencer and fluorescence sequencers and its applications.
Introduction.
Types of Protein – Protein Interaction.
Methods to investigate Protein – Protein Interaction.
Protein – Protein Interaction modulated by Chemical energy.
Two Hybrid Screening.
Overview of Protein – Protein Interaction analysis.
Biological effect of Protein – Protein interaction.
Conclusion.
Reference.
SYNTHETIC PEPTIDE VACCINES AND RECOMBINANT ANTIGEN VACCINED.R. Chandravanshi
What is a Vaccine?
A vaccine is a substance that is introduced into the body to prevent infection or to control disease due to a certain pathogen (a disease-causing organism, such as a virus, bacteria or parasite). The vaccine “teaches” the body how to defend itself against the pathogen by creating an immune response.
1 Unlike traditional pharmaceuticals, vaccines are biologics since they are made from living organisms (biological sources).
2 Specifically, vaccines are preparations of components derived from (or related to) a pathogen; they can typically induce a protective effect through one to three very small doses, in the range of micrograms to milligrams.
3 Immunity lasts for an extended period, from one year up to lifetime protection, including prevention of disease and/or related sequelae.
Synthetic peptide vaccines represent fragments of protein antigen sequences, synthesizing specific B cell and T cell epitopes offer the potential to induce diseases neutralizing immuno response with completely synthetic structure. Now it is well established that short chain peptides can be used to mimic antigenic sites of viruses and thus can be used the basics for vaccines and development. therefore, attempts have been made to synthesize such peptides which act as the serrogate immuunogens, as an alternative to the existing conventional vaccines.
What is Linux?
Command-line Interface, Shell & BASH
Popular commands
File Permissions and Owners
Installing programs
Piping and Scripting
Variables
Common applications in bioinformatics
Conclusion
The Superuser: Root
Disks and Partitions
Making New Partitions
Mounting Filesystems
Mounting a Filesystem: mount
Mounting Other Filesystems
Unmounting a Filesystem: umount
Sample /etc/fstab
Filesystem Types
Describe the application of DNA profiling in paternity tests and forensic investigations
Analyze DNA profiles to draw conclusions about paternity tests and forensic investigations.
M Pharm Pharmacognosy Semester 2, MEDICINAL PLANT BIOTECHNOLOGY UNIT 1, Introduction to Plant biotechnology: Historical perspectives, prospects for development of plant biotechnology as a source of
medicinal agents. Applications in pharmacy and allied fields. Genetic and molecular biology as applied to pharmacognosy, study of DNA, RNA and protein replication, genetic code, regulation of gene expression, structure and complicity of
genome, cell signaling, DNA recombinant technology.
This presentation explains the fundamentals of Genetic Code, Protein synthesis mechanism and Antibiotics that inhibits at various stages of Translation.
Instructions for Submissions thorugh G- Classroom.pptxJheel Barad
This presentation provides a briefing on how to upload submissions and documents in Google Classroom. It was prepared as part of an orientation for new Sainik School in-service teacher trainees. As a training officer, my goal is to ensure that you are comfortable and proficient with this essential tool for managing assignments and fostering student engagement.
The French Revolution, which began in 1789, was a period of radical social and political upheaval in France. It marked the decline of absolute monarchies, the rise of secular and democratic republics, and the eventual rise of Napoleon Bonaparte. This revolutionary period is crucial in understanding the transition from feudalism to modernity in Europe.
For more information, visit-www.vavaclasses.com
Acetabularia Information For Class 9 .docxvaibhavrinwa19
Acetabularia acetabulum is a single-celled green alga that in its vegetative state is morphologically differentiated into a basal rhizoid and an axially elongated stalk, which bears whorls of branching hairs. The single diploid nucleus resides in the rhizoid.
Honest Reviews of Tim Han LMA Course Program.pptxtimhan337
Personal development courses are widely available today, with each one promising life-changing outcomes. Tim Han’s Life Mastery Achievers (LMA) Course has drawn a lot of interest. In addition to offering my frank assessment of Success Insider’s LMA Course, this piece examines the course’s effects via a variety of Tim Han LMA course reviews and Success Insider comments.
Operation “Blue Star” is the only event in the history of Independent India where the state went into war with its own people. Even after about 40 years it is not clear if it was culmination of states anger over people of the region, a political game of power or start of dictatorial chapter in the democratic setup.
The people of Punjab felt alienated from main stream due to denial of their just demands during a long democratic struggle since independence. As it happen all over the word, it led to militant struggle with great loss of lives of military, police and civilian personnel. Killing of Indira Gandhi and massacre of innocent Sikhs in Delhi and other India cities was also associated with this movement.
June 3, 2024 Anti-Semitism Letter Sent to MIT President Kornbluth and MIT Cor...Levi Shapiro
Letter from the Congress of the United States regarding Anti-Semitism sent June 3rd to MIT President Sally Kornbluth, MIT Corp Chair, Mark Gorenberg
Dear Dr. Kornbluth and Mr. Gorenberg,
The US House of Representatives is deeply concerned by ongoing and pervasive acts of antisemitic
harassment and intimidation at the Massachusetts Institute of Technology (MIT). Failing to act decisively to ensure a safe learning environment for all students would be a grave dereliction of your responsibilities as President of MIT and Chair of the MIT Corporation.
This Congress will not stand idly by and allow an environment hostile to Jewish students to persist. The House believes that your institution is in violation of Title VI of the Civil Rights Act, and the inability or
unwillingness to rectify this violation through action requires accountability.
Postsecondary education is a unique opportunity for students to learn and have their ideas and beliefs challenged. However, universities receiving hundreds of millions of federal funds annually have denied
students that opportunity and have been hijacked to become venues for the promotion of terrorism, antisemitic harassment and intimidation, unlawful encampments, and in some cases, assaults and riots.
The House of Representatives will not countenance the use of federal funds to indoctrinate students into hateful, antisemitic, anti-American supporters of terrorism. Investigations into campus antisemitism by the Committee on Education and the Workforce and the Committee on Ways and Means have been expanded into a Congress-wide probe across all relevant jurisdictions to address this national crisis. The undersigned Committees will conduct oversight into the use of federal funds at MIT and its learning environment under authorities granted to each Committee.
• The Committee on Education and the Workforce has been investigating your institution since December 7, 2023. The Committee has broad jurisdiction over postsecondary education, including its compliance with Title VI of the Civil Rights Act, campus safety concerns over disruptions to the learning environment, and the awarding of federal student aid under the Higher Education Act.
• The Committee on Oversight and Accountability is investigating the sources of funding and other support flowing to groups espousing pro-Hamas propaganda and engaged in antisemitic harassment and intimidation of students. The Committee on Oversight and Accountability is the principal oversight committee of the US House of Representatives and has broad authority to investigate “any matter” at “any time” under House Rule X.
• The Committee on Ways and Means has been investigating several universities since November 15, 2023, when the Committee held a hearing entitled From Ivory Towers to Dark Corners: Investigating the Nexus Between Antisemitism, Tax-Exempt Universities, and Terror Financing. The Committee followed the hearing with letters to those institutions on January 10, 202
Read| The latest issue of The Challenger is here! We are thrilled to announce that our school paper has qualified for the NATIONAL SCHOOLS PRESS CONFERENCE (NSPC) 2024. Thank you for your unwavering support and trust. Dive into the stories that made us stand out!
Macroeconomics- Movie Location
This will be used as part of your Personal Professional Portfolio once graded.
Objective:
Prepare a presentation or a paper using research, basic comparative analysis, data organization and application of economic information. You will make an informed assessment of an economic climate outside of the United States to accomplish an entertainment industry objective.
2. 5.2 The Production of Protein
Until recently, proteins could only be made in cells.
3. 5.2 The Production of Protein
Until recently, proteins could only be made in cells.
Now small
polypeptide
chains can be
synthesized in
the laboratory.
Argonaut Technology
Quest 210 Protein
Synthesizer
4. 5.2 The Production of Protein
Overview of Protein Synthesis
Protein synthesis occurs continuously
throughout a cell’s life.
Protein synthesis is similar for eukaryotes
and prokaryotes.
Protein synthesis occurs on
ribosomes through a process
called translation.
5. 5.2 The Production of Protein
Overview of Protein Synthesis
Protein synthesis occurs continuously
throughout a cell’s life.
Protein synthesis is similar for eukaryotes
and prokaryotes.
Protein synthesis occurs on
ribosomes through a process
called translation.
6. 5.2 The Production of Protein
Overview of Protein Synthesis
Protein synthesis occurs continuously
throughout a cell’s life.
Protein synthesis is similar for eukaryotes
and prokaryotes.
Protein synthesis occurs on
ribosomes through a process
called translation.
7. Protein Synthesis in a Eukaryotic Cell.
In a eukaryotic cell, DNA is located within
chromosomes in the nucleus.
Transcription- RNA is transcribed from the
DNA template.
8. Protein Synthesis in a Eukaryotic Cell.
Introns are removed from the RNA and exons
are spliced together.
The exons comprise the mRNA (m for
messenger).
9. Protein Synthesis in a Eukaryotic Cell.
The mRNA transcripts carry the DNA code out
of the nucleus to the ribosomes, which translate
the code into a strand of amino acids.
10. Protein Synthesis in a Prokaryotic Cell.
There is no nucleus. There are no introns and
exons and the entire gene codes for protein.
Translation can begin before the mRNA is even
done being transcribed.
11. Transcription and Translation
Protein synthesis is a two-step process:
First Step:
Transcription
Genetic code must
be rewritten onto a
messenger
molecule.
RNA polymerase
attaches to the
promoter region of
a gene.
21. The GENETIC CODE
More than
one codon
for each a.a
Often the
third
nucleotide
can vary.
22. DNA MUTATIONS- Effect on proteins
Redundancy in the genetic code can
often make DNA point mutations (a
single nucleotide is changed) irrelevant.
Frame shift mutations- inserting or
deleting nucleotides that are not
multiples of threecause the greatest changes because
every a.a. after the mutation will be
wrong.
23. DNA MUTATIONS- Effect on proteins
Frame shift
mutations- inserting
or deleting
nucleotides that are
not multiples of
threecause the greatest
changes because
every a.a. after the
mutation will be
wrong.
24. Post-translational Modifications
Polypeptide chains fold into their 3D
conformations.
The protien may then be modified via
Glycosylation- addition of sugar groups
Phosphorylation- addition of
phosphate groups.
Cleavage- cut
25. Vocabulary
• Protein synthesis – the generation of new
proteins from amino acid subunits; in the cell, it
includes transcription and translation
• Transcription – the process of deciphering a DNA
nucleotide code and converting it into RNA
nucleotide code; the RNA carries the genetic message
to a ribosome for translation into a protein code
• Codon – a set of three nucleotides on a strand of
mRNA that codes for a particular amino acid
• Translation – the process of reading a mRNA
nucleotide code and converting it into a sequence of
amino acids
26. Vocabulary
• tRNA – a type of ribonucleic acid (RNA) that
shuttles amino acids into the ribosome for
protein synthesis
• Peptidyl transferase – an enzyme found in
the ribosome that builds polypeptide chains by
connecting amino acids into long chains through
peptide bonds
• Phosphorylation – adding phosphate groups
• Cleavage – process of splitting the polypeptide
into two or more strands
27. 5.2 Review Questions
1.
2.
3.
Distinguish between transcription and
translation.
If a structural gene’s code is “TAC GGC ATG
CCC TTA CGC ATC,” what will the mRNA
transcript be?
If the mRNA transcript from question No. 2
were translated into a peptide, what would
the amino-acid sequence of the peptide be?
28. Function of Antibody Proteins
Antibodies are proteins that recognize and bind
foreign molecules (antigens) for removal from
the body
29. Function of Antibody Proteins
Invasion by something foreign to the body
(an antigen) stimulates antibody production
by B lymphocytes (B cell).
30. Function of Antibody Proteins
Antibody proteins recognize a single shape on
an antigen called an epitope and bind there,
helping immune cells to recognize and attack
the antigen.
31. Function of Antibody Proteins
Antigens can be
•microorganisms (viruses, bacteria)
•microbial products (toxins)
•foreign proteins
•DNA and RNA molecules
•drugs
•other chemicals
32. Function of Antibody Proteins
Antibodies are also called immunoglobulins(Ig)
Most is IgG
33. Function of Antibody Proteins
Epitopes are the specific parts of antigens that
are recognized by antibodies.
•Each antibody recognizes a single epitope.
Multiple antibodies may
recognize and bind to
different epitopes on a
single antigen.
34. Function of Antibody Proteins
Epitopes are the specific parts of antigens that
are recognized by antibodies.
•Each antibody recognizes a single epitope.
•Multiple antibodies may
recognize and bind to
different epitopes on a
single antigen.
35. Function of Antibody Proteins
An HIV virus
particle (virion)
has many
potential epitopes
on its surface that
may be
recognized by
many different
antibodies.
36. Function of Antibody Proteins
Structure of IgG bound to the HIV capsid protein p24
as determined by X-ray crystallography.
(Harris et al.1998, Momany et al. 1996)
37. Antibodies are mass produced via many
methods.
polyclonal antibodiesa mixture of antibodies
for a single antigen
monoclonal antibodiesclones of a single
antibody
40. ELISA
(Enzyme-Linked Immunosorbent Assay)
-a useful form of analysis that exploits the
amazing specificity of antibodies to their
antigens.
Antibodies are
designed to bind
specific molecules
and produce a
visible color.
41. ELISA
(Enzyme-Linked Immunosorbent Assay)
-a useful form of analysis that exploits the
amazing specificity of antibodies to their
antigens.
Antibodies are
designed to bind
specific molecules
and produce a
visible color.
42. ELISA
Used for detecting all sorts of molecules and organisms
(Enzyme-Linked Immunosorbent Assay)
-HIV testing or any virus
-a useful form of -drug testing
analysis that exploits the
amazing specificity of antibodies to their
-pregnancy testing
-detection of allergens (gluten, soy, peanuts)
antigens.
-identify bacteria
-detect parasites
Antibodies are
- water contaminants
designed toGMO
bind
-detect
specific molecules
ELISA can also quantify - Tells HOW MUCH
and produce a
visible color.
43. Vocabulary
• Antigens – the foreign proteins or
molecules that are the target of binding by
antibodies
• Epitope – the specific region on a
molecule that an antibody binds to
44. Vocabulary
• ELISA – short for enzyme-linked
immunosorbent assay, a technique that
measures the amount of protein or antibody in
a solution
• Monoclonal antibody – a type of antibody
that is directed against a single epitope
• Hybridoma – a hybrid cell used to generate
monoclonal antibodies that results from the
fusion of immortal tumor cells with specific
antibody-producing white blood cells (B-cells)
45. 5.1 Review Questions
1.
2.
How many polypeptide chains are
found in an antibody, and how are they
held together in the protein?
What is the value of monoclonal
antibody technology?
46. The Importance of Proteins in
Biotech R&D
The ability to synthesize and modify peptides
or proteins is crucial to the production of
virtually every biotechnology product.
47. 5.3 Enzymes: Protein Catalysts
Enzymes and Their Substrates
Enzymes are proteins that act as catalysts.
Enzymes are involved in virtually every reaction in
a cell.
Many companies have focused on producing
enzymes for sale.
48. 5.3 Enzymes: Protein Catalysts
Enzymes and Their Substrates
The molecules upon which enzymes act are
called substrates.
49. 5.3 Enzymes: Protein Catalysts
Enzymes and Their Substrates
Enzyme active site and substrate match
exactly (the Lock and Key Model)
51. 5.3 Enzymes: Protein Catalysts
Factors That Affect Enzyme Activity
Amount of substrate in the solution
Temperature
Acidity or alkalinity
Enzymes have an optimum
temperature and pH.
52. 5.3 Enzymes: Protein Catalysts
Factors That Affect Enzyme Activity
Amount of substrate in the solution
Temperature
Acidity or alkalinity
Enzymes have an optimum
temperature and pH.
53. 5.3 Enzymes: Protein Catalysts
Factors That Affect Enzyme Activity
All proteins denature in extreme temps
and outside of their optimum pH.
54.
55. Vocabulary
• Substrate – the molecule that an enzyme acts on
• Lock and key model – a model used to
describe how enzymes function, in which the
enzyme and substrate make an exact molecular fit
at the active site, triggering catalysis
• Induced fit model – a model used to describe
how enzymes function, in which a substrate
squeezes into an active site and induces the
enzyme’s activity
56. Vocabulary
• Optimum temperature – the temperature
at which an enzyme achieves maximum activity
• Denaturation – the process in which
proteins lose their conformation or threedimensional shape
• Optimum pH – the pH at which an enzyme
achieves maximum activity
57. 5.4 Studying Proteins
A technician loads protein samples on a vertical gel.
Vertical gel boxes operate in a fashion similar to
horizontal gel boxes.
58. Vertical Gel Electrophoresis. Gel cassettes are snapped or
screwed in place (right). Running buffer is added behind the gel,
covering the wells. Buffer is poured in the front of the gel cassette
to cover the front opening. When the top is placed on the box
(left) and the power is turned on, electricity flows from the top
(negative charge) to bottom (positive charge). Negatively charged
samples move down the gel toward the positive electrode.
59. Silver stain is much more sensitive than Coomassie® Blue. When
samples have low concentrations of protein or DNA, silver-staining
is the method of choice.
60. 5.5 Applications of Protein Analysis
Protein profile of cells and tissues
A protein’s structure can help explain its
function
Study chemical processes in cells
Evolutionary and taxonomic relationships