DNA replication in prokaryotes occurs through a semi-discontinuous process at the replication fork. It begins with initiation proteins binding to the origin of replication and unwinding the DNA into single strands. DNA helicase then loads and unwinds the DNA further as single-strand binding proteins coat the exposed DNA. DNA primase adds RNA primers at the replication fork for DNA polymerase to begin DNA synthesis in the 5' to 3' direction. This results in continuous synthesis of the leading strand but discontinuous synthesis of the lagging strand as primers are repeatedly added. DNA ligase then seals the resulting Okazaki fragments on the lagging strand, completing replication of the bacterial chromosome.