This document provides an overview of UV-Visible spectroscopy. Some key points:
- UV-Vis spectroscopy involves promoting electrons from the ground state to excited states using electromagnetic radiation in the ultraviolet and visible regions.
- Different types of electronic transitions are possible including π-π*, n-π*, and σ-σ* transitions. The π-π* and n-π* transitions fall within the UV-Vis range.
- The wavelength of maximum absorbance (λmax) provides information about the energy gap between orbitals. Conjugated systems have longer λmax values and smaller energy gaps.
- Instruments use light sources, monochromators, sample and reference cells, and
Introduction,Instrumentation, Classification of electronic transitions, Substituent and solvent effects, Classification of electronic transitions
Substituent and solvent effects
Applications of UV Spectroscopy
UV spectral study of alkenes
UV spectral study of poylenes
UV spectral study of α, β-unsaturated carbonyl
UV spectral study of Aromatic compounds
Empirical rules for calculating λmax.
Applications of UV Spectroscopy, Empirical rules for calculating λmax.
Introduction,Instrumentation, Classification of electronic transitions, Substituent and solvent effects, Classification of electronic transitions
Substituent and solvent effects
Applications of UV Spectroscopy
UV spectral study of alkenes
UV spectral study of poylenes
UV spectral study of α, β-unsaturated carbonyl
UV spectral study of Aromatic compounds
Empirical rules for calculating λmax.
Applications of UV Spectroscopy, Empirical rules for calculating λmax.
Spectroscopy is the branch of science dealing the study of interaction of electromagnetic radiation with matter. OR
It is the measurement of electromagnetic radiation (EMR) absorbed or emitted when molecule or ions or atoms of a sample move from one energy state to another energy state.
Spectroscopy is the most powerful tool available for the study of atomic & molecular structure and is used in the analysis of a wide range of samples .
IR SPECTROSCOPY, INTRODUCTION, PRINCIPLE, THEORY, FATE OF ABSORBED RADIATION, FERMI RESONANCE, FINGERPRINT REGION, VIBRATIONS, FACTORS AFFECTING ABSORPTION OF IR RADIATION, SAMPLING TECHNIQUES, APPLICATIONS OF IR SPECTROSCOPY.
This presentation gives you thorough knowledge about the IR Spectroscopy. This include basic principle, type of vibrations, factors influencing vibrational frequency, instrumentation and applications of IR Spectroscopy. This is the most widely used technique for identifying unknown functional group depending on the vibrational frequency.
It would be use full to All Needy People. It involve information about NMR Spectroscopy ( a spectroscopic techniques), factors influencing , proton NMR and their applications of NMR as well as Nuclear magnetic imaging.
Spectroscopy is the branch of science dealing the study of interaction of electromagnetic radiation with matter. OR
It is the measurement of electromagnetic radiation (EMR) absorbed or emitted when molecule or ions or atoms of a sample move from one energy state to another energy state.
Spectroscopy is the most powerful tool available for the study of atomic & molecular structure and is used in the analysis of a wide range of samples .
IR SPECTROSCOPY, INTRODUCTION, PRINCIPLE, THEORY, FATE OF ABSORBED RADIATION, FERMI RESONANCE, FINGERPRINT REGION, VIBRATIONS, FACTORS AFFECTING ABSORPTION OF IR RADIATION, SAMPLING TECHNIQUES, APPLICATIONS OF IR SPECTROSCOPY.
This presentation gives you thorough knowledge about the IR Spectroscopy. This include basic principle, type of vibrations, factors influencing vibrational frequency, instrumentation and applications of IR Spectroscopy. This is the most widely used technique for identifying unknown functional group depending on the vibrational frequency.
It would be use full to All Needy People. It involve information about NMR Spectroscopy ( a spectroscopic techniques), factors influencing , proton NMR and their applications of NMR as well as Nuclear magnetic imaging.
spectrophotometry, ultra violet absorption, infra red atomic absorption.priya tamang
A spectrophotometer is a photometer that can measure the intensity of light as a function of its wavelength. Single beam and double beam are the two major classes of spectrophotometers. Linear range of absorption and spectral bandwidth measurement are the important features of spectrophotometers.
In Single Beam Spectrophotometers, all the light passes through the sample. To measure the intensity of the incident light the sample must be removed so that all the light can pass through. This type of spectrometer is usually less expensive and less complicated. The single beam instruments are optically simpler and more compact, znc can also have a larger dynamic range.
In a Double Beam Spectrophotometer, before it reaches the sample, the light source is split into two separate beams. One beam passes through the sample and the second one is used for reference. This gives an advantage because the reference reading and sample reading can take place at the same time.
In transmission measurements, the spectrophotometer quantitatively compares the amount of light passing through the reference and test sample. For reflectance, it compares the amount of light reflecting from the test and reference sample solutions.
Many spectrophotometers must be calibrated before they start to analyse the sample and the procedure for calibrating spectrophotometer is known as "zeroing." Calibration is done by using the reference substance, and the absorbencies of all other substances are measured relative to the reference substance. % transmissivity (the amount of light transmitted through the substance relative to the initial substance) is displayed on the spectrophotometer.
uv-visible spectroscopy also available video lecture on youtube channel name ...Pharma Rising, Bhopal
This slide contain introduction, electromagnetic radiation, lamberts beers law, principal, instrumentation, application of uv visible spectroscopy
also contain data interpretation and difference and factor which affect absorption
absorption shift and effects
bhutnashak is india's number one constipation churna .its action starts within 2 to 3 hours after oral use.it provides relief from all types of constipation.it is non habit forming and recommended for all family members.
Title: Sense of Smell
Presenter: Dr. Faiza, Assistant Professor of Physiology
Qualifications:
MBBS (Best Graduate, AIMC Lahore)
FCPS Physiology
ICMT, CHPE, DHPE (STMU)
MPH (GC University, Faisalabad)
MBA (Virtual University of Pakistan)
Learning Objectives:
Describe the primary categories of smells and the concept of odor blindness.
Explain the structure and location of the olfactory membrane and mucosa, including the types and roles of cells involved in olfaction.
Describe the pathway and mechanisms of olfactory signal transmission from the olfactory receptors to the brain.
Illustrate the biochemical cascade triggered by odorant binding to olfactory receptors, including the role of G-proteins and second messengers in generating an action potential.
Identify different types of olfactory disorders such as anosmia, hyposmia, hyperosmia, and dysosmia, including their potential causes.
Key Topics:
Olfactory Genes:
3% of the human genome accounts for olfactory genes.
400 genes for odorant receptors.
Olfactory Membrane:
Located in the superior part of the nasal cavity.
Medially: Folds downward along the superior septum.
Laterally: Folds over the superior turbinate and upper surface of the middle turbinate.
Total surface area: 5-10 square centimeters.
Olfactory Mucosa:
Olfactory Cells: Bipolar nerve cells derived from the CNS (100 million), with 4-25 olfactory cilia per cell.
Sustentacular Cells: Produce mucus and maintain ionic and molecular environment.
Basal Cells: Replace worn-out olfactory cells with an average lifespan of 1-2 months.
Bowman’s Gland: Secretes mucus.
Stimulation of Olfactory Cells:
Odorant dissolves in mucus and attaches to receptors on olfactory cilia.
Involves a cascade effect through G-proteins and second messengers, leading to depolarization and action potential generation in the olfactory nerve.
Quality of a Good Odorant:
Small (3-20 Carbon atoms), volatile, water-soluble, and lipid-soluble.
Facilitated by odorant-binding proteins in mucus.
Membrane Potential and Action Potential:
Resting membrane potential: -55mV.
Action potential frequency in the olfactory nerve increases with odorant strength.
Adaptation Towards the Sense of Smell:
Rapid adaptation within the first second, with further slow adaptation.
Psychological adaptation greater than receptor adaptation, involving feedback inhibition from the central nervous system.
Primary Sensations of Smell:
Camphoraceous, Musky, Floral, Pepperminty, Ethereal, Pungent, Putrid.
Odor Detection Threshold:
Examples: Hydrogen sulfide (0.0005 ppm), Methyl-mercaptan (0.002 ppm).
Some toxic substances are odorless at lethal concentrations.
Characteristics of Smell:
Odor blindness for single substances due to lack of appropriate receptor protein.
Behavioral and emotional influences of smell.
Transmission of Olfactory Signals:
From olfactory cells to glomeruli in the olfactory bulb, involving lateral inhibition.
Primitive, less old, and new olfactory systems with different path
TEST BANK for Operations Management, 14th Edition by William J. Stevenson, Ve...kevinkariuki227
TEST BANK for Operations Management, 14th Edition by William J. Stevenson, Verified Chapters 1 - 19, Complete Newest Version.pdf
TEST BANK for Operations Management, 14th Edition by William J. Stevenson, Verified Chapters 1 - 19, Complete Newest Version.pdf
Prix Galien International 2024 Forum ProgramLevi Shapiro
June 20, 2024, Prix Galien International and Jerusalem Ethics Forum in ROME. Detailed agenda including panels:
- ADVANCES IN CARDIOLOGY: A NEW PARADIGM IS COMING
- WOMEN’S HEALTH: FERTILITY PRESERVATION
- WHAT’S NEW IN THE TREATMENT OF INFECTIOUS,
ONCOLOGICAL AND INFLAMMATORY SKIN DISEASES?
- ARTIFICIAL INTELLIGENCE AND ETHICS
- GENE THERAPY
- BEYOND BORDERS: GLOBAL INITIATIVES FOR DEMOCRATIZING LIFE SCIENCE TECHNOLOGIES AND PROMOTING ACCESS TO HEALTHCARE
- ETHICAL CHALLENGES IN LIFE SCIENCES
- Prix Galien International Awards Ceremony
Pulmonary Thromboembolism - etilogy, types, medical- Surgical and nursing man...VarunMahajani
Disruption of blood supply to lung alveoli due to blockage of one or more pulmonary blood vessels is called as Pulmonary thromboembolism. In this presentation we will discuss its causes, types and its management in depth.
micro teaching on communication m.sc nursing.pdfAnurag Sharma
Microteaching is a unique model of practice teaching. It is a viable instrument for the. desired change in the teaching behavior or the behavior potential which, in specified types of real. classroom situations, tends to facilitate the achievement of specified types of objectives.
Anti ulcer drugs and their Advance pharmacology ||
Anti-ulcer drugs are medications used to prevent and treat ulcers in the stomach and upper part of the small intestine (duodenal ulcers). These ulcers are often caused by an imbalance between stomach acid and the mucosal lining, which protects the stomach lining.
||Scope: Overview of various classes of anti-ulcer drugs, their mechanisms of action, indications, side effects, and clinical considerations.
Lung Cancer: Artificial Intelligence, Synergetics, Complex System Analysis, S...Oleg Kshivets
RESULTS: Overall life span (LS) was 2252.1±1742.5 days and cumulative 5-year survival (5YS) reached 73.2%, 10 years – 64.8%, 20 years – 42.5%. 513 LCP lived more than 5 years (LS=3124.6±1525.6 days), 148 LCP – more than 10 years (LS=5054.4±1504.1 days).199 LCP died because of LC (LS=562.7±374.5 days). 5YS of LCP after bi/lobectomies was significantly superior in comparison with LCP after pneumonectomies (78.1% vs.63.7%, P=0.00001 by log-rank test). AT significantly improved 5YS (66.3% vs. 34.8%) (P=0.00000 by log-rank test) only for LCP with N1-2. Cox modeling displayed that 5YS of LCP significantly depended on: phase transition (PT) early-invasive LC in terms of synergetics, PT N0—N12, cell ratio factors (ratio between cancer cells- CC and blood cells subpopulations), G1-3, histology, glucose, AT, blood cell circuit, prothrombin index, heparin tolerance, recalcification time (P=0.000-0.038). Neural networks, genetic algorithm selection and bootstrap simulation revealed relationships between 5YS and PT early-invasive LC (rank=1), PT N0—N12 (rank=2), thrombocytes/CC (3), erythrocytes/CC (4), eosinophils/CC (5), healthy cells/CC (6), lymphocytes/CC (7), segmented neutrophils/CC (8), stick neutrophils/CC (9), monocytes/CC (10); leucocytes/CC (11). Correct prediction of 5YS was 100% by neural networks computing (area under ROC curve=1.0; error=0.0).
CONCLUSIONS: 5YS of LCP after radical procedures significantly depended on: 1) PT early-invasive cancer; 2) PT N0--N12; 3) cell ratio factors; 4) blood cell circuit; 5) biochemical factors; 6) hemostasis system; 7) AT; 8) LC characteristics; 9) LC cell dynamics; 10) surgery type: lobectomy/pneumonectomy; 11) anthropometric data. Optimal diagnosis and treatment strategies for LC are: 1) screening and early detection of LC; 2) availability of experienced thoracic surgeons because of complexity of radical procedures; 3) aggressive en block surgery and adequate lymph node dissection for completeness; 4) precise prediction; 5) adjuvant chemoimmunoradiotherapy for LCP with unfavorable prognosis.
The prostate is an exocrine gland of the male mammalian reproductive system
It is a walnut-sized gland that forms part of the male reproductive system and is located in front of the rectum and just below the urinary bladder
Function is to store and secrete a clear, slightly alkaline fluid that constitutes 10-30% of the volume of the seminal fluid that along with the spermatozoa, constitutes semen
A healthy human prostate measures (4cm-vertical, by 3cm-horizontal, 2cm ant-post ).
It surrounds the urethra just below the urinary bladder. It has anterior, median, posterior and two lateral lobes
It’s work is regulated by androgens which are responsible for male sex characteristics
Generalised disease of the prostate due to hormonal derangement which leads to non malignant enlargement of the gland (increase in the number of epithelial cells and stromal tissue)to cause compression of the urethra leading to symptoms (LUTS
These lecture slides, by Dr Sidra Arshad, offer a quick overview of physiological basis of a normal electrocardiogram.
Learning objectives:
1. Define an electrocardiogram (ECG) and electrocardiography
2. Describe how dipoles generated by the heart produce the waveforms of the ECG
3. Describe the components of a normal electrocardiogram of a typical bipolar leads (limb II)
4. Differentiate between intervals and segments
5. Enlist some common indications for obtaining an ECG
Study Resources:
1. Chapter 11, Guyton and Hall Textbook of Medical Physiology, 14th edition
2. Chapter 9, Human Physiology - From Cells to Systems, Lauralee Sherwood, 9th edition
3. Chapter 29, Ganong’s Review of Medical Physiology, 26th edition
4. Electrocardiogram, StatPearls - https://www.ncbi.nlm.nih.gov/books/NBK549803/
5. ECG in Medical Practice by ABM Abdullah, 4th edition
6. ECG Basics, http://www.nataliescasebook.com/tag/e-c-g-basics
- Video recording of this lecture in English language: https://youtu.be/lK81BzxMqdo
- Video recording of this lecture in Arabic language: https://youtu.be/Ve4P0COk9OI
- Link to download the book free: https://nephrotube.blogspot.com/p/nephrotube-nephrology-books.html
- Link to NephroTube website: www.NephroTube.com
- Link to NephroTube social media accounts: https://nephrotube.blogspot.com/p/join-nephrotube-on-social-media.html
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These simplified slides by Dr. Sidra Arshad present an overview of the non-respiratory functions of the respiratory tract.
Learning objectives:
1. Enlist the non-respiratory functions of the respiratory tract
2. Briefly explain how these functions are carried out
3. Discuss the significance of dead space
4. Differentiate between minute ventilation and alveolar ventilation
5. Describe the cough and sneeze reflexes
Study Resources:
1. Chapter 39, Guyton and Hall Textbook of Medical Physiology, 14th edition
2. Chapter 34, Ganong’s Review of Medical Physiology, 26th edition
3. Chapter 17, Human Physiology by Lauralee Sherwood, 9th edition
4. Non-respiratory functions of the lungs https://academic.oup.com/bjaed/article/13/3/98/278874
MANAGEMENT OF ATRIOVENTRICULAR CONDUCTION BLOCK.pdfJim Jacob Roy
Cardiac conduction defects can occur due to various causes.
Atrioventricular conduction blocks ( AV blocks ) are classified into 3 types.
This document describes the acute management of AV block.
2. Spectroscopy is the study of the interaction
between matter and electromagnetic
radiation.
spectroscopy originated through the study
of visible light dispersed according to
its wavelength, by a prism.
Later the concept was expanded greatly to
include any interaction with irradiative
energy as a function of its wavelength or
frequency.
3. The word spectroscopy implies that we will use the
electromagnetic spectrum to gain information about
organic molecules.
The other name of UV (Ultra-Violet) spectroscopy is
Electronic spectroscopy as it involves the promotion of the
electrons from the ground state to the higher energy or
excited state.
ultraviolet means that the information will come from a
specific region of the electromagnetic spectrum called the
ultraviolet region. The electromagnetic spectrum includes
all radiation that travels at the speed of light c (3 x 1010
cm/sec). The electromagnetic spectrum includes radio
waves, which have long wavelengths, x-rays, which have
short wavelengths, and visible light, which has
wavelengths between those of radio waves and x-rays.
4. x-rays are most energetic, visible light next,
and radio waves least energetic.
Thus, the shorter the wavelength, the
greater the energy of an electromagnetic
wave.
The heat excites some ground-state
electrons to higher energy levels, then when
the electrons “fall” back to the ground state,
they “emit” energy that corresponds to the
energy difference between the energy states
(orbitals) where the electrons are found
5.
6.
7.
8. The UV-Vis Spectrometer
The basic idea behind UV-Vis Spectroscopy is to shine light
of varying wavelengths through a sample and to measure
the absorbance at each wavelength. Only the wavelengths
corresponding to the ΔE for an electronic transition will be
strongly absorbed.
A UV-Vis spectrum plots absorbance (or its inverse,
transmittance) of the sample versus wavelength
9. Here’s the spectrum for ethene. [In this case the wavelength is plotted
versus transmittance, the inverse of absorbance (high absorbance = low transmittance,
and vice versa). ]
Note that the wavelength of maximum transmittance is at 174 nm. We call
this λmax , pronounced “lambda max”. Very little light passes through the sample at this
wavelength, because the wavelength corresponds very closely to ΔE for the π to π*
transition.
10. For example, knowing that the λmax for ethene is at
174 nm allows us to calculate the energy gap ΔE ,
which turns out to be about 164 kcal/mol.
11. As the number of conjugated pi bonds increases, the λmax increases as well!
Because longer frequency = smaller energy, this means that the energy
gap ΔE between the highest-occupied molecular orbital (HOMO) and lowest
unoccupied molecular orbital (LUMO)decreases as the number of conjugated pi
bonds increases
12. The ultraviolet region falls in the range
between 190-380 nm, the visible region fall
between 380-750 nm.
The following electronic transitions are
possible:
π- π* (pi to pi star transition)
n - π* (n to pi star transition)
σ - σ * (sigma to sigma star transition)
n - σ * (n to sigma star transition)
and are shown in the below hypothetical
energy diagram
13. The σ to σ* transition requires an absorption of a photon with a wavelength which
does not fall in the UV-vis range (see table 2 below). Thus, only π to π* and n to π*
transitions occur in the UV-vis region are observed.
14. σ to σ* > n to σ* > π to π* > n to π*
highest energy lowest energy
(lowest wavelength) (lowest wavelength)
σ to σ*= c-c(alkanes)
π to π*=c=c ,or triple bond(alkenes, alkynes)
n to π*=c=o(carbonyl compounds)
15. How Does λmax Relate To The Color We
Perceive?
How does the wavelength of maximum absorbance (λmax)
relate to the actual color?
First, a refresher from the last post. We see the
complementary colour of the major color that is
absorbed. A molecule that absorbs in the blue will
appear orange, because we perceive the colors that
are reflected, and orange is the complementary color of
blue.
For example, this molecule, Rhodamine B [note 2] absorbs
at about 560 nm (green) and appears red , the
complimentary color of green.
19. Introduction to UV spectroscopy-
UV spectroscopy is type of absorption
spectroscopy in which light of ultra-violet region
(200-400 nm.) is absorbed by the molecule.
Absorption of the ultra-violet radiations results in
the excitation of the electrons from the ground
state to higher energy state.
The energy of the ultra-violet radiation that are
absorbed is equal to the energy difference between
the ground state and higher energy states (deltaE =
hf).
20. Generally, the most favored transition is from the
highest occupied molecular orbital (HOMO) to lowest
unoccupied molecular orbital (LUMO).
For most of the molecules, the lowest energy occupied
molecular orbitals are s orbital, which correspond to
sigma bonds.
The p orbitals are at somewhat higher energy levels,
the orbitals (nonbonding orbitals) with unshared paired
of electrons lie at higher energy levels.
The unoccupied or antibonding orbitals (pie*and
sigma*) are the highest energy occupied orbitals.
Some of the important transitions with increasing
energies are: nonbonding to pie*, nonbonding to sigma*,
pie to pie*, sigma to pie* and sigma to sigma*.
21. Principle of UV spectroscopy
UV spectroscopy obeys the Beer-Lambert law, which states that: when a beam of
monochromatic light is passed through a solution of an absorbing substance, the
rate of decrease of intensity of radiation with thickness of the absorbing solution
is proportional to the incident radiation as well as the concentration of the
solution.
The expression of Beer-Lambert law is-
A = log (I0/I) = Ecl
Where, A = absorbance
I0 = intensity of light incident upon sample cell
I = intensity of light leaving sample cell
C = molar concentration of solute
L = length of sample cell (cm.)
E = molar absorptivity
From the Beer-Lambert law it is clear that greater the number of molecules
capable of absorbing light of a given wavelength, the greater the extent of light
absorption. This is the basic principle of UV spectroscopy.
22. Instrumentation and working of UV
Light Source- Tungsten filament lamps and Hydrogen-Deuterium
lamps are most widely used and suitable light source as they cover the
whole UV region. Tungsten filament lamps are rich in red radiations;
more specifically they emit the radiations of 375 nm, while the
intensity of Hydrogen-Deuterium lamps falls below 375 nm.
Monochromator- Monochromators generally composed of prisms
and slits. The most of the spectrophotometers are double beam
spectrophotometers. The radiation emitted from the primary source
is dispersed with the help of rotating prisms. The various wavelengths
of the light source which are separated by the prism are then selected
by the slits such the rotation of the prism results in a series of
continuously increasing wavelength to pass through the slits for
recording purpose. The beam selected by the slit is monochromatic
and further divided into two beams with the help of another prism.
23. Sample and reference cells- One of the two divided
beams is passed through the sample solution and second
beam is passé through the reference solution. Both
sample and reference solution are contained in the cells.
These cells are made of either silica or quartz. Glass can't
be used for the cells as it also absorbs light in the UV
region.
Detector- Generally two photocells serve the purpose of
detector in UV spectroscopy. One of the photocell
receives the beam from sample cell and second detector
receives the beam from the reference. The intensity of the
radiation from the reference cell is stronger than the
beam of sample cell. This results in the generation of
pulsating or alternating currents in the photocells.
24. Amplifier- The alternating current generated in the
photocells is transferred to the amplifier. The
amplifier is coupled to a small servometer. Generally
current generated in the photocells is of very low
intensity, the main purpose of amplifier is to amplify
the signals many times so we can get clear and
recordable signals.
Recording devices- Most of the time amplifier is
coupled to a pen recorder which is connected to the
computer. Computer stores all the data generated
and produces the spectrum of the desired
compound
25.
26.
27. Concept of Chromophore and Auxochrome in the UV
Chromophore- Chromophore is defined as any isolated covalently bonded group that
shows a characteristic absorption in the ultraviolet or visible region (200-800 nm).
Chromophores can be divided into two groups-
a) Chromophores which contain p electrons and which undergo pie to
pie* transitions. Ethylenes and acetylenes are the example of such chromophores.
b) Chromophores which contain both p and nonbonding electrons. They undergo two
types of transitions; pie to pie* and nonbonding to pie*. Carbonyl, nitriles, azo
compounds, nitro compounds etc. are the example of such chromophores.
Auxochromes- An auxochrome can be defined as any group which does not itself act
as a chromophore but whose presence brings about a shift of the absorption band
towards the longer wavelength of the spectrum. –OH,-OR,-NH2,-NHR, -SH etc. are the
examples of auxochromic groups.
28. Absorption and intensity shifts in the UV spectroscopy
a) Bathochromic effect- This type of shift is also known as red shift. Bathochromic shift is an
effect by virtue of which the absorption maximum is shifted towards the longer wavelength
due to the presence of an auxochrome or change in solvents.
The nonbonding to pie* transition of carbonyl compounds observes bathochromic or red
shift.
b) Hypsochromic shift- This effect is also known as blue shift. Hypsochromic shift is an effect
by virtue of which absorption maximum is shifted towards the shorter wavelength. Generally
it is caused due to the removal of conjugation or by changing the polarity of the solvents.
c) Hyperchromic effect- Hyperchromic shift is an effect by virtue of which absorption
maximum increases. The introduction of an auxochrome in the compound generally results
in the hyperchromic effect.
d) Hypochromic effect- Hyperchromic effect is defined as the effect by virtue of intensity of
absorption maximum decreases. Hyperchromic effect occurs due to the distortion of the
geometry of the molecule with an introduction of new group.
31. Solvent Effect
Solvents play an important role in UV
spectra. Compound peak could be obscured
by the solvent peak. So a most suitable
solvent is one that does not itself get
absorbed in the region under investigation.
A solvent should be transparent in a
particular region. A dilute solution of sample
is always prepared for analysis. Most
commonly used solvents are as follows.
33. Applications of UV spectroscopy
1. Detection of functional groups- UV spectroscopy is used to detect the presence
or absence of chromophore in the compound. This is technique is not useful for the
detection of chromophore in complex compounds. The absence of a band at a
particular band can be seen as an evidence for the absence of a particular group. If
the spectrum of a compound comes out to be transparent above 200 nm than it
confirms the absence of –
a) Conjugation b) A carbonyl group c) Benzene or aromatic compound d) Bromo or
iodo atoms.
2. Detection of extent of conjugation- The extent of conjugation in the polyenes
can be detected with the help of UV spectroscopy. With the increase in double
bonds the absorption shifts towards the longer wavelength. If the double bond is
increased by 8 in the polyenes then that polyene appears visible to the human eye
as the absorption comes in the visible region.
34. 3. Identification of an unknown compound- An unknown compound
can be identified with the help of UV spectroscopy. The spectrum of
unknown compound is compared with the spectrum of a reference
compound and if both the spectrums coincide then it confirms the
identification of the unknown substance.
4. Determination of configurations of geometrical isomers- It is
observed that cis-alkenes absorb at different wavelength than the
trans-alkenes. The two isomers can be distinguished with each other
when one of the isomers has non-coplanar structure due to steric
hindrances. The cis-isomer suffers distortion and absorbs at lower
wavelength as compared to trans-isomer.
5. Determination of the purity of a substance- Purity of a substance
can also be determined with the help of UV spectroscopy. The
absorption of the sample solution is compared with the absorption of
the reference solution. The intensity of the absorption can be used for
the relative calculation of the purity of the sample substance.