UV-visible spectroscopy involves using electromagnetic radiation in the ultraviolet-visible spectral region to analyze molecules. It works by exciting electrons within molecules from their ground state to excited states. The position and intensity of absorption peaks in the UV spectrum depend on factors like the type of chromophore, conjugation, solvent, and pH. UV spectroscopy is used to determine molecular weights, identify functional groups and conjugation, quantify concentrations, and characterize aromatic compounds.