The document discusses ultracentrifugation, which uses high centrifugal forces to separate particles in solutions based on size, shape, and density. It describes: 1) How particles experience centrifugal, buoyant, and frictional forces when spun in an ultracentrifuge. 2) Key terms like sedimentation rate, sedimentation coefficient, and angular velocity. 3) Types of ultracentrifugation experiments like sedimentation velocity and equilibrium experiments. 4) Types of preparative ultracentrifugation like differential, density gradient, zonal, and isopycnic centrifugation used to separate cell components. 5) Components of an ultracentrifuge like rotors, buckets,