This document describes Fast Protein Liquid Chromatography (FPLC), which is a chromatography system used to separate proteins and other biomolecules. FPLC uses stationary phases like resins and mobile phases like buffers. It works on the principles of size exclusion, ion exchange, and affinity chromatography. The key components of an FPLC system include pumps, mixers, injection valves, columns, fraction collectors, and detectors. Compared to HPLC, FPLC uses lower pressures, inert materials, and larger columns. Its advantages include reproducibility, simple operation, and suitability for analytical and preparative purposes. Common applications of FPLC include protein analysis, purification of venoms and plasma proteins.