SlideShare a Scribd company logo
CENTRIFUGATION
Submitted by
SHOBHANA SAHU
M.Sc. 4th semester
1
SYNOPSIS
• Introduction
• Definition
• Components of centrifuge
• Types of centrifugation
• Application
• Conclusion
• References
2
INTRODUCTION
• Centrifugation is a process which involves the
use of the centrifugal force for the
sedimentation of heterogeneous mixtures with
a centrifuge, used in industry and laboratory
settings.
• This process is used to separate two
immiscible liquids.
3
4
DEFINITION
• A centrifugation is a
technique for
separating particles
from a solution
according to their
size, shape, density,
viscosity of the
medium and rotor
speed.
5
CENTRIFUGE
• A centrifuge is a part
of equipment that puts
an object in rotation
around a fixed axis
(spin in a circle),
applying a potentially
strong force
perpendicular to the
axis of spin.
6
PRINCIPLE
• In a solution, particles whose density is higher than
that of the solvent sink (sediment), and particles that
are lighter than it float to the top.
• The greater the difference in density, the faster they
move. If there is no difference in density (isopycnic
conditions), the particles stay steady.
• To take advantage of even tiny differences in density
to separate various particles in a solution, gravity can
be replaced with the much more powerful
“centrifugal force” provided by a centrifuge.
7
COMPONENTS OF CENTRIFUGE
• Motor- which give rotator motion.
• Drive shaft- attached to motor.
• Rotor- placed on drive shaft having space to
place tubes.
• Centrifuge rotor: a rotor is the rotating unit of
the centrifuge, which has fixed holes drilled at
an angle. Test tubes are placed inside these
holes and the rotor spins to aid in the
separation of the materials.
8
9
10
11
TYPES OF CENTRIFUGATION
Preparative centrifugation:
• it is a preliminary step to separate component for
further separation and analysis. It can handle large
amount of samples. Its divide 2 types:
• Density gradient centrifugation
• Differential velocity centrifugation
Analytical centrifugation:
• we analyze the sample so small amount is used
few micro-gram/ L to 1 mL
• Mostly ultracentrifuge is used
12
DENSITY GRADIENT CENTRIFUGATION
• Under centrifugal force, the particles will
begin sedimenting through the density
gradient.
• Two type of this-
1. Rate zonal centrifugation
2. Isopycnic centrifugation
13
Rate zonal centrifugation
14
15
Differential velocity centrifugation
16
17
18
19
ULTRACENTRIFUGATION
• The ultracentrifuge is a centrifuge optimized
for spinning a rotor at very high speeds,
capable of generating acceleration as high as
19600 km/s² (around 50000 rpm).
• Example: Red cells may be separated from
plasma of blood, nuclei from mitochondria in
cell homogenates, and one protein from
another in complex mixtures.
20
ULTRACENTRIFUGATION
21
22
CALCULATION FOR CENTRIFUGATION
23
24
APPLICATION OF CENTRIFUGATION
• Isolating suspensions
• Isotope separation
• Aeronautics and astronautics
• Geotechnical centrifuge modeling:
• Commercial applications:
25
HUMAN CENTRIFUGE
26
27
28
CONCLUSION
• The centrifugation is a modern and easy
technique of separation.
• Due to centrifugation it is easy to separate
cellular and sub cellular components.
• It is used to study the effects of centrifugal
forces on cells.
• But it is costly and problem to maintenance.
29
REFERENCES
• https://www.slideshare.net/poojakamble1609/principles-and-applications-of-centrifugation
• https://www.slideshare.net/poojakamble1609/principles-and-applications-of-centrifugation
• https://www.britannica.com/technology/centrifuge
• https://www.sigmaaldrich.com/technical-documents/articles/biofiles/centrifugation-separations.html
• http://www.centrifuge.guide/significant-applications-of-centrifuges/
• http://centrifuges.hitachi-koki.com/application/pdf/red153e.pdf
• http://www.phys.sinica.edu.tw/TIGP-
NANO/Course/2007_Spring/Class%20Notes/AC_Chapter%203%20Centrifugation%200321.pdf
30
Thank
you
Any
quer
ies?
31

More Related Content

What's hot

Centrifugation principle and types by Dr. Anurag Yadav
Centrifugation principle and types by Dr. Anurag YadavCentrifugation principle and types by Dr. Anurag Yadav
Centrifugation principle and types by Dr. Anurag Yadav
Dr Anurag Yadav
 
Centrifugation
CentrifugationCentrifugation
Centrifugation
Oyshe Ahmed
 
Centrifugation
CentrifugationCentrifugation
Ultracentrifugation
UltracentrifugationUltracentrifugation
Ultracentrifugation
Sabahat Ali
 
Principles and applications of centrifugation ppt
Principles and applications of centrifugation pptPrinciples and applications of centrifugation ppt
Principles and applications of centrifugation ppt
poojakamble1609
 
B.Sc Biotech II BAT Unit 2 Centrifugation
B.Sc Biotech II BAT Unit 2 CentrifugationB.Sc Biotech II BAT Unit 2 Centrifugation
B.Sc Biotech II BAT Unit 2 Centrifugation
Rai University
 
Centrifugation
Centrifugation Centrifugation
Centrifugation
Rahul Lodha
 
Analytical centrifugation
Analytical centrifugationAnalytical centrifugation
Analytical centrifugation
Varshini3
 
Centrifugation techniques
Centrifugation techniquesCentrifugation techniques
Centrifugation techniques
Amany Elsayed
 
Centrifugation
CentrifugationCentrifugation
Centrifugation
sri lakshmi sai
 
Centrifugation
CentrifugationCentrifugation
Centrifugation
farzeen javaid
 
Differential centrifugation
Differential centrifugationDifferential centrifugation
Differential centrifugation
SuganyaPaulraj
 
Centrifugation and types of rotors
Centrifugation and types of rotorsCentrifugation and types of rotors
Centrifugation and types of rotors
Mahek Sharan
 
Density gradient centrifugation,types, advantages and disadvantages
Density gradient centrifugation,types, advantages and disadvantages Density gradient centrifugation,types, advantages and disadvantages
Density gradient centrifugation,types, advantages and disadvantages
MUSKANKr
 
Preparative and analytical centrifugation
Preparative and analytical centrifugationPreparative and analytical centrifugation
Preparative and analytical centrifugation
Pratheeba Subramani
 
Cell fractionation
Cell fractionationCell fractionation
Cell fractionation
Prof Viyatprajna Acharya
 
Cell Organelle Fractionation
Cell Organelle Fractionation Cell Organelle Fractionation
Cell Organelle Fractionation
JayashreeShanmugam14
 
differential centrifugation
differential centrifugationdifferential centrifugation
differential centrifugation
Rakshmitha Marni
 
Centrifugation
CentrifugationCentrifugation
Centrifugation
Nishant kumar
 

What's hot (20)

Centrifugation principle and types by Dr. Anurag Yadav
Centrifugation principle and types by Dr. Anurag YadavCentrifugation principle and types by Dr. Anurag Yadav
Centrifugation principle and types by Dr. Anurag Yadav
 
Centrifugation
CentrifugationCentrifugation
Centrifugation
 
Centrifugation
CentrifugationCentrifugation
Centrifugation
 
Ultracentrifugation
UltracentrifugationUltracentrifugation
Ultracentrifugation
 
Principles and applications of centrifugation ppt
Principles and applications of centrifugation pptPrinciples and applications of centrifugation ppt
Principles and applications of centrifugation ppt
 
B.Sc Biotech II BAT Unit 2 Centrifugation
B.Sc Biotech II BAT Unit 2 CentrifugationB.Sc Biotech II BAT Unit 2 Centrifugation
B.Sc Biotech II BAT Unit 2 Centrifugation
 
Centrifugation
Centrifugation Centrifugation
Centrifugation
 
centrifuge
centrifugecentrifuge
centrifuge
 
Analytical centrifugation
Analytical centrifugationAnalytical centrifugation
Analytical centrifugation
 
Centrifugation techniques
Centrifugation techniquesCentrifugation techniques
Centrifugation techniques
 
Centrifugation
CentrifugationCentrifugation
Centrifugation
 
Centrifugation
CentrifugationCentrifugation
Centrifugation
 
Differential centrifugation
Differential centrifugationDifferential centrifugation
Differential centrifugation
 
Centrifugation and types of rotors
Centrifugation and types of rotorsCentrifugation and types of rotors
Centrifugation and types of rotors
 
Density gradient centrifugation,types, advantages and disadvantages
Density gradient centrifugation,types, advantages and disadvantages Density gradient centrifugation,types, advantages and disadvantages
Density gradient centrifugation,types, advantages and disadvantages
 
Preparative and analytical centrifugation
Preparative and analytical centrifugationPreparative and analytical centrifugation
Preparative and analytical centrifugation
 
Cell fractionation
Cell fractionationCell fractionation
Cell fractionation
 
Cell Organelle Fractionation
Cell Organelle Fractionation Cell Organelle Fractionation
Cell Organelle Fractionation
 
differential centrifugation
differential centrifugationdifferential centrifugation
differential centrifugation
 
Centrifugation
CentrifugationCentrifugation
Centrifugation
 

Similar to Ppt of centrifugation

Centrifugation techniques by kk sahu
Centrifugation techniques by kk sahuCentrifugation techniques by kk sahu
Centrifugation techniques by kk sahu
KAUSHAL SAHU
 
Centrifugation
CentrifugationCentrifugation
Centrifugation
Bibatsu
 
Centrifugation
CentrifugationCentrifugation
Centrifugation
Arushi Yashi
 
Centrifugation Presentation.pptx
Centrifugation Presentation.pptxCentrifugation Presentation.pptx
Centrifugation Presentation.pptx
AnishaKazi
 
Centrifuge.pptx
Centrifuge.pptxCentrifuge.pptx
Centrifuge.pptx
saraso888
 
Centrifugation
CentrifugationCentrifugation
Centrifugation
Pharmacy Universe
 
Centrifugation-Basic study
Centrifugation-Basic studyCentrifugation-Basic study
Centrifugation-Basic study
Dr.Prameswari Kasa
 
Extraction techniques
Extraction techniquesExtraction techniques
Extraction techniques
Hafiz M Waseem
 
Extraction techniques(cetrifugation)
Extraction techniques(cetrifugation)Extraction techniques(cetrifugation)
Extraction techniques(cetrifugation)
Hafiz M Waseem
 
Centrifugation notes slideshare
Centrifugation notes slideshare Centrifugation notes slideshare
Centrifugation notes slideshare
strawverrymilktae
 
Centrifugation.
Centrifugation.Centrifugation.
Centrifugation.
BhavikaKatariya
 
Centrifugation
CentrifugationCentrifugation
Centrifugation
Umangi Thakkar
 
Centrifugation
CentrifugationCentrifugation
Centrifugation
ShahedShadin
 
Centrifugation basic principle & theory
Centrifugation basic principle & theoryCentrifugation basic principle & theory
Centrifugation basic principle & theory
Mayank Sagar
 
BioMedical Instrumentation-II What is centrifugation? Basic principle of ce...
BioMedical Instrumentation-II What is  centrifugation? Basic principle of  ce...BioMedical Instrumentation-II What is  centrifugation? Basic principle of  ce...
BioMedical Instrumentation-II What is centrifugation? Basic principle of ce...
The University of Lahore
 
Centrifugation amirtham
Centrifugation   amirthamCentrifugation   amirtham
Centrifugation amirtham
christanantony
 
Prvn ppt
Prvn pptPrvn ppt
Prvn ppt
praveenpkd22
 
prvnppt-141230053156-conversion-gate02.pdf
prvnppt-141230053156-conversion-gate02.pdfprvnppt-141230053156-conversion-gate02.pdf
prvnppt-141230053156-conversion-gate02.pdf
DevPanda5
 
62 bioanalatyical.pptx
62 bioanalatyical.pptx62 bioanalatyical.pptx
62 bioanalatyical.pptx
DevaprasadPanda
 
Pipettes and centrifuge with centrifugation
Pipettes and centrifuge with centrifugation Pipettes and centrifuge with centrifugation
Pipettes and centrifuge with centrifugation
Hari Sharan Makaju
 

Similar to Ppt of centrifugation (20)

Centrifugation techniques by kk sahu
Centrifugation techniques by kk sahuCentrifugation techniques by kk sahu
Centrifugation techniques by kk sahu
 
Centrifugation
CentrifugationCentrifugation
Centrifugation
 
Centrifugation
CentrifugationCentrifugation
Centrifugation
 
Centrifugation Presentation.pptx
Centrifugation Presentation.pptxCentrifugation Presentation.pptx
Centrifugation Presentation.pptx
 
Centrifuge.pptx
Centrifuge.pptxCentrifuge.pptx
Centrifuge.pptx
 
Centrifugation
CentrifugationCentrifugation
Centrifugation
 
Centrifugation-Basic study
Centrifugation-Basic studyCentrifugation-Basic study
Centrifugation-Basic study
 
Extraction techniques
Extraction techniquesExtraction techniques
Extraction techniques
 
Extraction techniques(cetrifugation)
Extraction techniques(cetrifugation)Extraction techniques(cetrifugation)
Extraction techniques(cetrifugation)
 
Centrifugation notes slideshare
Centrifugation notes slideshare Centrifugation notes slideshare
Centrifugation notes slideshare
 
Centrifugation.
Centrifugation.Centrifugation.
Centrifugation.
 
Centrifugation
CentrifugationCentrifugation
Centrifugation
 
Centrifugation
CentrifugationCentrifugation
Centrifugation
 
Centrifugation basic principle & theory
Centrifugation basic principle & theoryCentrifugation basic principle & theory
Centrifugation basic principle & theory
 
BioMedical Instrumentation-II What is centrifugation? Basic principle of ce...
BioMedical Instrumentation-II What is  centrifugation? Basic principle of  ce...BioMedical Instrumentation-II What is  centrifugation? Basic principle of  ce...
BioMedical Instrumentation-II What is centrifugation? Basic principle of ce...
 
Centrifugation amirtham
Centrifugation   amirthamCentrifugation   amirtham
Centrifugation amirtham
 
Prvn ppt
Prvn pptPrvn ppt
Prvn ppt
 
prvnppt-141230053156-conversion-gate02.pdf
prvnppt-141230053156-conversion-gate02.pdfprvnppt-141230053156-conversion-gate02.pdf
prvnppt-141230053156-conversion-gate02.pdf
 
62 bioanalatyical.pptx
62 bioanalatyical.pptx62 bioanalatyical.pptx
62 bioanalatyical.pptx
 
Pipettes and centrifuge with centrifugation
Pipettes and centrifuge with centrifugation Pipettes and centrifuge with centrifugation
Pipettes and centrifuge with centrifugation
 

Recently uploaded

June 3, 2024 Anti-Semitism Letter Sent to MIT President Kornbluth and MIT Cor...
June 3, 2024 Anti-Semitism Letter Sent to MIT President Kornbluth and MIT Cor...June 3, 2024 Anti-Semitism Letter Sent to MIT President Kornbluth and MIT Cor...
June 3, 2024 Anti-Semitism Letter Sent to MIT President Kornbluth and MIT Cor...
Levi Shapiro
 
Embracing GenAI - A Strategic Imperative
Embracing GenAI - A Strategic ImperativeEmbracing GenAI - A Strategic Imperative
Embracing GenAI - A Strategic Imperative
Peter Windle
 
Synthetic Fiber Construction in lab .pptx
Synthetic Fiber Construction in lab .pptxSynthetic Fiber Construction in lab .pptx
Synthetic Fiber Construction in lab .pptx
Pavel ( NSTU)
 
Acetabularia Information For Class 9 .docx
Acetabularia Information For Class 9  .docxAcetabularia Information For Class 9  .docx
Acetabularia Information For Class 9 .docx
vaibhavrinwa19
 
Supporting (UKRI) OA monographs at Salford.pptx
Supporting (UKRI) OA monographs at Salford.pptxSupporting (UKRI) OA monographs at Salford.pptx
Supporting (UKRI) OA monographs at Salford.pptx
Jisc
 
The geography of Taylor Swift - some ideas
The geography of Taylor Swift - some ideasThe geography of Taylor Swift - some ideas
The geography of Taylor Swift - some ideas
GeoBlogs
 
678020731-Sumas-y-Restas-Para-Colorear.pdf
678020731-Sumas-y-Restas-Para-Colorear.pdf678020731-Sumas-y-Restas-Para-Colorear.pdf
678020731-Sumas-y-Restas-Para-Colorear.pdf
CarlosHernanMontoyab2
 
The Accursed House by Émile Gaboriau.pptx
The Accursed House by Émile Gaboriau.pptxThe Accursed House by Émile Gaboriau.pptx
The Accursed House by Émile Gaboriau.pptx
DhatriParmar
 
Palestine last event orientationfvgnh .pptx
Palestine last event orientationfvgnh .pptxPalestine last event orientationfvgnh .pptx
Palestine last event orientationfvgnh .pptx
RaedMohamed3
 
A Strategic Approach: GenAI in Education
A Strategic Approach: GenAI in EducationA Strategic Approach: GenAI in Education
A Strategic Approach: GenAI in Education
Peter Windle
 
Francesca Gottschalk - How can education support child empowerment.pptx
Francesca Gottschalk - How can education support child empowerment.pptxFrancesca Gottschalk - How can education support child empowerment.pptx
Francesca Gottschalk - How can education support child empowerment.pptx
EduSkills OECD
 
1.4 modern child centered education - mahatma gandhi-2.pptx
1.4 modern child centered education - mahatma gandhi-2.pptx1.4 modern child centered education - mahatma gandhi-2.pptx
1.4 modern child centered education - mahatma gandhi-2.pptx
JosvitaDsouza2
 
Unit 2- Research Aptitude (UGC NET Paper I).pdf
Unit 2- Research Aptitude (UGC NET Paper I).pdfUnit 2- Research Aptitude (UGC NET Paper I).pdf
Unit 2- Research Aptitude (UGC NET Paper I).pdf
Thiyagu K
 
Adversarial Attention Modeling for Multi-dimensional Emotion Regression.pdf
Adversarial Attention Modeling for Multi-dimensional Emotion Regression.pdfAdversarial Attention Modeling for Multi-dimensional Emotion Regression.pdf
Adversarial Attention Modeling for Multi-dimensional Emotion Regression.pdf
Po-Chuan Chen
 
Operation Blue Star - Saka Neela Tara
Operation Blue Star   -  Saka Neela TaraOperation Blue Star   -  Saka Neela Tara
Operation Blue Star - Saka Neela Tara
Balvir Singh
 
Home assignment II on Spectroscopy 2024 Answers.pdf
Home assignment II on Spectroscopy 2024 Answers.pdfHome assignment II on Spectroscopy 2024 Answers.pdf
Home assignment II on Spectroscopy 2024 Answers.pdf
Tamralipta Mahavidyalaya
 
Introduction to AI for Nonprofits with Tapp Network
Introduction to AI for Nonprofits with Tapp NetworkIntroduction to AI for Nonprofits with Tapp Network
Introduction to AI for Nonprofits with Tapp Network
TechSoup
 
Model Attribute Check Company Auto Property
Model Attribute  Check Company Auto PropertyModel Attribute  Check Company Auto Property
Model Attribute Check Company Auto Property
Celine George
 
Thesis Statement for students diagnonsed withADHD.ppt
Thesis Statement for students diagnonsed withADHD.pptThesis Statement for students diagnonsed withADHD.ppt
Thesis Statement for students diagnonsed withADHD.ppt
EverAndrsGuerraGuerr
 
Guidance_and_Counselling.pdf B.Ed. 4th Semester
Guidance_and_Counselling.pdf B.Ed. 4th SemesterGuidance_and_Counselling.pdf B.Ed. 4th Semester
Guidance_and_Counselling.pdf B.Ed. 4th Semester
Atul Kumar Singh
 

Recently uploaded (20)

June 3, 2024 Anti-Semitism Letter Sent to MIT President Kornbluth and MIT Cor...
June 3, 2024 Anti-Semitism Letter Sent to MIT President Kornbluth and MIT Cor...June 3, 2024 Anti-Semitism Letter Sent to MIT President Kornbluth and MIT Cor...
June 3, 2024 Anti-Semitism Letter Sent to MIT President Kornbluth and MIT Cor...
 
Embracing GenAI - A Strategic Imperative
Embracing GenAI - A Strategic ImperativeEmbracing GenAI - A Strategic Imperative
Embracing GenAI - A Strategic Imperative
 
Synthetic Fiber Construction in lab .pptx
Synthetic Fiber Construction in lab .pptxSynthetic Fiber Construction in lab .pptx
Synthetic Fiber Construction in lab .pptx
 
Acetabularia Information For Class 9 .docx
Acetabularia Information For Class 9  .docxAcetabularia Information For Class 9  .docx
Acetabularia Information For Class 9 .docx
 
Supporting (UKRI) OA monographs at Salford.pptx
Supporting (UKRI) OA monographs at Salford.pptxSupporting (UKRI) OA monographs at Salford.pptx
Supporting (UKRI) OA monographs at Salford.pptx
 
The geography of Taylor Swift - some ideas
The geography of Taylor Swift - some ideasThe geography of Taylor Swift - some ideas
The geography of Taylor Swift - some ideas
 
678020731-Sumas-y-Restas-Para-Colorear.pdf
678020731-Sumas-y-Restas-Para-Colorear.pdf678020731-Sumas-y-Restas-Para-Colorear.pdf
678020731-Sumas-y-Restas-Para-Colorear.pdf
 
The Accursed House by Émile Gaboriau.pptx
The Accursed House by Émile Gaboriau.pptxThe Accursed House by Émile Gaboriau.pptx
The Accursed House by Émile Gaboriau.pptx
 
Palestine last event orientationfvgnh .pptx
Palestine last event orientationfvgnh .pptxPalestine last event orientationfvgnh .pptx
Palestine last event orientationfvgnh .pptx
 
A Strategic Approach: GenAI in Education
A Strategic Approach: GenAI in EducationA Strategic Approach: GenAI in Education
A Strategic Approach: GenAI in Education
 
Francesca Gottschalk - How can education support child empowerment.pptx
Francesca Gottschalk - How can education support child empowerment.pptxFrancesca Gottschalk - How can education support child empowerment.pptx
Francesca Gottschalk - How can education support child empowerment.pptx
 
1.4 modern child centered education - mahatma gandhi-2.pptx
1.4 modern child centered education - mahatma gandhi-2.pptx1.4 modern child centered education - mahatma gandhi-2.pptx
1.4 modern child centered education - mahatma gandhi-2.pptx
 
Unit 2- Research Aptitude (UGC NET Paper I).pdf
Unit 2- Research Aptitude (UGC NET Paper I).pdfUnit 2- Research Aptitude (UGC NET Paper I).pdf
Unit 2- Research Aptitude (UGC NET Paper I).pdf
 
Adversarial Attention Modeling for Multi-dimensional Emotion Regression.pdf
Adversarial Attention Modeling for Multi-dimensional Emotion Regression.pdfAdversarial Attention Modeling for Multi-dimensional Emotion Regression.pdf
Adversarial Attention Modeling for Multi-dimensional Emotion Regression.pdf
 
Operation Blue Star - Saka Neela Tara
Operation Blue Star   -  Saka Neela TaraOperation Blue Star   -  Saka Neela Tara
Operation Blue Star - Saka Neela Tara
 
Home assignment II on Spectroscopy 2024 Answers.pdf
Home assignment II on Spectroscopy 2024 Answers.pdfHome assignment II on Spectroscopy 2024 Answers.pdf
Home assignment II on Spectroscopy 2024 Answers.pdf
 
Introduction to AI for Nonprofits with Tapp Network
Introduction to AI for Nonprofits with Tapp NetworkIntroduction to AI for Nonprofits with Tapp Network
Introduction to AI for Nonprofits with Tapp Network
 
Model Attribute Check Company Auto Property
Model Attribute  Check Company Auto PropertyModel Attribute  Check Company Auto Property
Model Attribute Check Company Auto Property
 
Thesis Statement for students diagnonsed withADHD.ppt
Thesis Statement for students diagnonsed withADHD.pptThesis Statement for students diagnonsed withADHD.ppt
Thesis Statement for students diagnonsed withADHD.ppt
 
Guidance_and_Counselling.pdf B.Ed. 4th Semester
Guidance_and_Counselling.pdf B.Ed. 4th SemesterGuidance_and_Counselling.pdf B.Ed. 4th Semester
Guidance_and_Counselling.pdf B.Ed. 4th Semester
 

Ppt of centrifugation