This document summarizes tissue engineering approaches for repairing spinal cord injuries. It discusses the use of stem cells (especially neural stem cells, Schwann cells, bone marrow stem cells, and adipose-derived stem cells) as seed cells. Scaffolds made from materials like silk fibroin, chitosan, collagen, and hydrogels are described. Growth factors that could be delivered include NGF, BDNF, and NT-3 to promote neuronal survival and axon regeneration. While tissue engineering is promising for spinal cord repair, more research is still needed to develop fully successful treatment methods.
iPSCs are pluripotent; unlike ESC, iPSCs are not derived from the embryo, but instead created from differentiated cells in the lab through a process – cellular reprogramming.
iPSCs are pluripotent; unlike ESC, iPSCs are not derived from the embryo, but instead created from differentiated cells in the lab through a process – cellular reprogramming.
If the cell is able to form all cell types of the embryo & adult (Fertilized egg cell) Totipotent stem cell
Stem cell able to differentiate into all 3 germ layers Pluripotent stem cell (Embryonic stem cell)
Multipotent stem cell Differentiate to form cells of some but not all 3 germ layers (Bone, cartilage, connective tissue)
Unipotent stem cell Able to form just one other cell type (Spermatogonia)
Embryos created in vitro fertilization
Aborted embryos
Limited tissues (bone marrow, muscle, brain)
Discrete populations of adult stem cells generate replacements for cells that are lost through normal wear and tear, injury or disease
Placental cord
Baby teeth
Diabetes patients lose the function of their insulin-producing beta cells of the pancreas
Human embryonic stem cells may be grown in cell cultures and stimulate to form insulin-producing cells , that can be transplanted into the patients
Pancreas is digested with collagenase that frees islets from surrounding cells
Centrifugation of isolates containing mainly alpha and beta cells, purified islets beta cells
Transplanted through a catheter into the liver where they become permanently established Caused when key brain cells that produce message carrying chemical/neurotransmitter (dopamine) die off.
Symptoms start with the patients trembling and can end up paralyzed
Harvesting of stem cells from patients bone marrow, foetus or any other source
Culturing of harvested stem cells in lab conditions - to get high concentrations of stem cells
Then purified and high concentration of stem cells are surgically injected in the brain of patient.
PRODUCTION AND MAINTENANCE OF EMBRYONIC STEM CELLSANKUR SHARMA
Embryonic stem cells are pluripotent stem cells and have capacity to differentiate into all type of cells arising from 3 different germ layers i.e., ecto-, meso- and endoderm. In this presentation brief information is given about different methods for production of embryonic stem cells and their maintenance
If the cell is able to form all cell types of the embryo & adult (Fertilized egg cell) Totipotent stem cell
Stem cell able to differentiate into all 3 germ layers Pluripotent stem cell (Embryonic stem cell)
Multipotent stem cell Differentiate to form cells of some but not all 3 germ layers (Bone, cartilage, connective tissue)
Unipotent stem cell Able to form just one other cell type (Spermatogonia)
Embryos created in vitro fertilization
Aborted embryos
Limited tissues (bone marrow, muscle, brain)
Discrete populations of adult stem cells generate replacements for cells that are lost through normal wear and tear, injury or disease
Placental cord
Baby teeth
Diabetes patients lose the function of their insulin-producing beta cells of the pancreas
Human embryonic stem cells may be grown in cell cultures and stimulate to form insulin-producing cells , that can be transplanted into the patients
Pancreas is digested with collagenase that frees islets from surrounding cells
Centrifugation of isolates containing mainly alpha and beta cells, purified islets beta cells
Transplanted through a catheter into the liver where they become permanently established Caused when key brain cells that produce message carrying chemical/neurotransmitter (dopamine) die off.
Symptoms start with the patients trembling and can end up paralyzed
Harvesting of stem cells from patients bone marrow, foetus or any other source
Culturing of harvested stem cells in lab conditions - to get high concentrations of stem cells
Then purified and high concentration of stem cells are surgically injected in the brain of patient.
PRODUCTION AND MAINTENANCE OF EMBRYONIC STEM CELLSANKUR SHARMA
Embryonic stem cells are pluripotent stem cells and have capacity to differentiate into all type of cells arising from 3 different germ layers i.e., ecto-, meso- and endoderm. In this presentation brief information is given about different methods for production of embryonic stem cells and their maintenance
How Stem Cell Therapy is Transforming Stroke Rehabilitation | Dr. David GreeneR3 Stem Cell
Stroke is a leading cause of long-term disability, affecting millions of people worldwide. Stem cell therapy offers a promising approach to enhance recovery and improve the quality of life for stroke survivors. In this presentation, Dr. David Greene R3 Stem Cell will explore the transformative impact of stem cell therapy in stroke rehabilitation. Visit our website for more information.
Journal club: iPSC derived myelinoids to study myelin biology of humansLeena Shingavi
This is a journal club presentation on the manuscript by Owen et al, published in May 2021 in the journal Developmental cell. It describes how the myelinoids were derived from the iPSCs and can be considered as model to study myelin biology.
Pathophysiology of TBI is complex and consists of acute and delayed injury. In the acute phase, brain tissue destroyed upon impact includes neurons, glia, and endothelial cells, the latter of which makes up the blood-brain barrier. In the delayed phase, “toxins” released from damaged cells set off cascades in neighboring cells eventually leading to exacerbation of primary injury. As researches further explore pathophysiology and molecular mechanisms underlying this debilitating condition, numerous potential therapeutic strategies, especially those involving stem cells, are emerging to improve recovery and possibly reverse damage. In addition to elucidating the most recent advances in the understanding of TBI pathophysiology, this review explores two primary pathways currently under investigation and are thought to yield the most viable therapeutic approach for treatment of TBI: manipulation of endogenous neural cell response and administration of exogenous stem cell therapy.
The prostate is an exocrine gland of the male mammalian reproductive system
It is a walnut-sized gland that forms part of the male reproductive system and is located in front of the rectum and just below the urinary bladder
Function is to store and secrete a clear, slightly alkaline fluid that constitutes 10-30% of the volume of the seminal fluid that along with the spermatozoa, constitutes semen
A healthy human prostate measures (4cm-vertical, by 3cm-horizontal, 2cm ant-post ).
It surrounds the urethra just below the urinary bladder. It has anterior, median, posterior and two lateral lobes
It’s work is regulated by androgens which are responsible for male sex characteristics
Generalised disease of the prostate due to hormonal derangement which leads to non malignant enlargement of the gland (increase in the number of epithelial cells and stromal tissue)to cause compression of the urethra leading to symptoms (LUTS
MANAGEMENT OF ATRIOVENTRICULAR CONDUCTION BLOCK.pdfJim Jacob Roy
Cardiac conduction defects can occur due to various causes.
Atrioventricular conduction blocks ( AV blocks ) are classified into 3 types.
This document describes the acute management of AV block.
Anti ulcer drugs and their Advance pharmacology ||
Anti-ulcer drugs are medications used to prevent and treat ulcers in the stomach and upper part of the small intestine (duodenal ulcers). These ulcers are often caused by an imbalance between stomach acid and the mucosal lining, which protects the stomach lining.
||Scope: Overview of various classes of anti-ulcer drugs, their mechanisms of action, indications, side effects, and clinical considerations.
micro teaching on communication m.sc nursing.pdfAnurag Sharma
Microteaching is a unique model of practice teaching. It is a viable instrument for the. desired change in the teaching behavior or the behavior potential which, in specified types of real. classroom situations, tends to facilitate the achievement of specified types of objectives.
ARTIFICIAL INTELLIGENCE IN HEALTHCARE.pdfAnujkumaranit
Artificial intelligence (AI) refers to the simulation of human intelligence processes by machines, especially computer systems. It encompasses tasks such as learning, reasoning, problem-solving, perception, and language understanding. AI technologies are revolutionizing various fields, from healthcare to finance, by enabling machines to perform tasks that typically require human intelligence.
Lung Cancer: Artificial Intelligence, Synergetics, Complex System Analysis, S...Oleg Kshivets
RESULTS: Overall life span (LS) was 2252.1±1742.5 days and cumulative 5-year survival (5YS) reached 73.2%, 10 years – 64.8%, 20 years – 42.5%. 513 LCP lived more than 5 years (LS=3124.6±1525.6 days), 148 LCP – more than 10 years (LS=5054.4±1504.1 days).199 LCP died because of LC (LS=562.7±374.5 days). 5YS of LCP after bi/lobectomies was significantly superior in comparison with LCP after pneumonectomies (78.1% vs.63.7%, P=0.00001 by log-rank test). AT significantly improved 5YS (66.3% vs. 34.8%) (P=0.00000 by log-rank test) only for LCP with N1-2. Cox modeling displayed that 5YS of LCP significantly depended on: phase transition (PT) early-invasive LC in terms of synergetics, PT N0—N12, cell ratio factors (ratio between cancer cells- CC and blood cells subpopulations), G1-3, histology, glucose, AT, blood cell circuit, prothrombin index, heparin tolerance, recalcification time (P=0.000-0.038). Neural networks, genetic algorithm selection and bootstrap simulation revealed relationships between 5YS and PT early-invasive LC (rank=1), PT N0—N12 (rank=2), thrombocytes/CC (3), erythrocytes/CC (4), eosinophils/CC (5), healthy cells/CC (6), lymphocytes/CC (7), segmented neutrophils/CC (8), stick neutrophils/CC (9), monocytes/CC (10); leucocytes/CC (11). Correct prediction of 5YS was 100% by neural networks computing (area under ROC curve=1.0; error=0.0).
CONCLUSIONS: 5YS of LCP after radical procedures significantly depended on: 1) PT early-invasive cancer; 2) PT N0--N12; 3) cell ratio factors; 4) blood cell circuit; 5) biochemical factors; 6) hemostasis system; 7) AT; 8) LC characteristics; 9) LC cell dynamics; 10) surgery type: lobectomy/pneumonectomy; 11) anthropometric data. Optimal diagnosis and treatment strategies for LC are: 1) screening and early detection of LC; 2) availability of experienced thoracic surgeons because of complexity of radical procedures; 3) aggressive en block surgery and adequate lymph node dissection for completeness; 4) precise prediction; 5) adjuvant chemoimmunoradiotherapy for LCP with unfavorable prognosis.
Explore natural remedies for syphilis treatment in Singapore. Discover alternative therapies, herbal remedies, and lifestyle changes that may complement conventional treatments. Learn about holistic approaches to managing syphilis symptoms and supporting overall health.
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Couples presenting to the infertility clinic- Do they really have infertility...Sujoy Dasgupta
Dr Sujoy Dasgupta presented the study on "Couples presenting to the infertility clinic- Do they really have infertility? – The unexplored stories of non-consummation" in the 13th Congress of the Asia Pacific Initiative on Reproduction (ASPIRE 2024) at Manila on 24 May, 2024.
4. INTRODUCTION
• Spinal cord injury (SCI) may lead to a devastating and
permanent loss of neurological function that significally
affects the mobility and quality of life in adults (
Average age of patient is 38 years old ).
• SCI is a complex set of damaging events that occur at
the the cellular level and it can be divided into four
main stages, they are
• The immediate
• Acute Stage
• Intermediate
• Chronic Phase
5. • Clinically, the treatment of SCI mainly focuses on
reducing secondary damage and prevention of
further complications.
• To successfully repair the SCI and promote functional
recovery, the following must be achieved.
1. Reduction of the death of neuronal cells
2. Inhibition of glial scar formation, since glial scaring
decreases axon growth
3. Provision of matrix at the injury site to supply the nutrients
required to support axonal growth
4. Elimination of immune reactions
5. Facilitation of the build-up of functional synapses and the
transmission of neurotransmitters by regenerating axons.
6. TISSUE ENGINEERING IN THE
REPAIR OF SCI
• Tissue engineering is a promising method
that may be used for the treatment of SCI.
• It involves three factors:
1)The Seed Cell,
2)Scaffold,
3)Growth factors
• For repair strategy to be successful, the
selection of an appropriate seed cell,
scaffold and growth factor is required.
8. SEED CELL
In Tissue Engineering Seed Cells must meet
the following criteria:
• Successful ability to proliferation in vitro
• Good cell viability and function
• High level of purity
• No rejection by the immune system and
• High safety
9. • Embryonic Stem Cell and Neural Stem Cells are
the most important types of stems cells that
were used in the early stages of seed cell
research.
• Transplanting ES Cells into the brains was
shown to significantly improve neurological
function in an animal model of Parkinsons’
disease.
• The main reason for the success is that cells
survived and were differentiated into different
cells, such as Oligodendrocytes, Astrocytes
and Neurons
11. NEURAL DIFFERENTIATION OF
EMBRYONIC STEM CELL
• This method has certain ethical issues
and problems with regard to rejection
reactions.
• In addition the availability of neural stem
cells is limited and therefore their
widespread clinical use is not viable.
12. SCHWANN CELLS:
• Schwann Cells originating from dorsal
and central roots are one of the cellular
components that migrate to the site of
issue damage after spinal cord injury.
NEURAL STEM PROGENITOR CELLS:
• A scaffold seeded with NSCs for repairing
CNS lesions can provide a platform for
the cells enabling repair of large neural
defects.
14. BONE MARROW STEM CELLS:
• With advances in stem cell research Mesenchymal Stem
Cells (MSCs) extracted from Bone Marrow (BMSCs)
have been shown to contain pluripotent precursor cells,
which have the ability to differentiate into various types
of brain cell.
• In vivo transplantation of BMSCs into the brain has
established that they migrate throughout various brain
regions where they undergo differentiation into cells
with astrocytic and neuronal phenotypes.
• If BMSCs are to be used clinically, the extraction of
bone marrow from patients is necessary which result in
Pateint Trauma. Therefore, on increasing number of
studies have suggested the use of adult stem cells.
15. ADULT STEM CELLS – ADIPOSE
DERIVED STEM CELLS
• Adipose derived stem cells (ADSCs) is the most
suitable type of adult stem cells for use as seed
cells of SCI treatment.
• An advantage of using ADSCs is that obtaining
these cells is minimally invasive to the patient.
• If different types of induction medium are used,
the cells may differentiate in to adipocytes,
oesteoblasts, chondrocytes and neurons, a
promising tool for SCI treatment.
16. • Recent studies have shown that due to the
secretion of various growth factors such as
hepatocyte growth factor (HGF), Tumor
necrosis factor – Alpha (TNF-Alpha),
Vascular endothelial growth factors (VEGF),
Brain-derived neurotropic factor (BDNF)
and Nerve Growth Factor (NGF).
• ADSCs may be used in the acute stages of SCI
and have the potential to improve functional
recovery, tissue preservation and neurenal
regeneration.
17. SCAFFOLD
• In addition to seed cells, tissue engineering
scaffolds are also important and their potential
use in the spinal repair.
• The requirements of a scaffold for spinal cord
regeneration are as follows:
• Good bio-compatibility, in order to avoid
reactions with the immune systems
• An ideal degradation rate and the formation of
non-toxic degradation products.
• Mechanical properties that are suitable for cell
adhesion and axonal re-growth.
18. • In order to successfully use tissue
engineering to repair SCI the selection of
suitable scaffold is particularly important.
• Compared with a single component scaffold,
a mixed scaffold may be more successful as
it may minimize the disadvantages of the
single component scaffold and provide a
scaffold with increased functionality.
19. • Silk fibroin (SF) has excellent mechanical properties and
has been used as a scaffold for the treatment of SCI but the
disadvantage is that when it is dry it is brittle and difficult
to handle
• To overcome the shortcomings of SF, a blend of both
chitosan and Silk Fibroin to make silk fibroin – chitosan
(SFCS) has good mechanical properties and may be used as
a scaffold material for the repair of SCIs.
• A study by Liu et al using nanofibrous collagen nerve
conduits demonstrated that this type of scaffold is capable
of promoting neural fiber growth following SCI, and is also
capable of inhibiting glial scar hyperplasia.
• A study by Du et al demonstrated that a gelatin sponge is
more suitable that an PLGA scaffold for transplantation
into the spinal cord to promote the recovery of SCI.
20. • Comolle et al used a poly (N-isopropylacrylamide) –co-
poly (ethylene glycol) (PNIPAAM-PEG) injection
scaffold, which provided the sustained release of BDNF
and neurotrophin-3 (NT-3) for up to four weeks, the
constant secretion of these GFs was identified to be a
positive factor in functional recovery.
• Scaffolds prepared from natural components may be
more advantageous as the reaction of the immune system
and the inflammatory reaction is reduced following
implantation into the body.
• The blending of artificial and synthetic materials may
reduce the disadvantage of using synthetic material while
also avoiding the disadvantages of using solely natural
components
21. GROWTH FACTOR
• Neurotrophic factors play an important role
in the functional recovery following SCI, as they
protect neuronal cells from apoptosis and
promote axonal regeneration.
• Neurotrophic factors may be divided into:
– Neurotrophins
– Ciliary neurotrophic factor
– Glial cell line – derived neurotrophic factors
– Other growth factors or cytokines
• The most frequently used neurotrophic factors
are NGF, NT-B, and BDNF.
22. • NGF is a core factor in the regulation of peripheral
innervoctions, was found to have an effect on the
CNS
• Brain-derived neurotrophic factor
(BDNF)expresses its biological effects through the
activation and binding of TrkB.
• Stokols et al discovered that a BDNF-
incorporated agarose scaffold implanted into
the spinal cord of a rat resulted in the linear –
fashioned growth of regenerating axons through
the scaffold
23. • NT-3 which may be generated by the cloning of
a multifunctional NGF gene not only
maintains motor neurons, sympathetic neurons
and dopaminergic neuron differentiation, but
also maintains the survival of sympathetic and
sensory neurons and promotes growth of nerve
in vitro.
• NT-3 is considered to be the only gene to
promote the growth of the Corticospinal
tract (CST) following SCI.
24. CONCLUSION
• Tissue engineering is a promising method that
may be used for the treatment of SCI.
• It involves three factors: the seed cell, the
scaffold and a growth factor.
• For the repair strategy to be successful, the
selection of an appropriate seed cell, scaffold
and growth factor is required.
• In conclusion, although tissue engineering has
a promising future for the treatment of SCI,
extensive further studies are necessary for the
successful treatment of SCI to be achieved.