Stem cells for dental tissue engineering

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Stem cells for dental tissue engineering

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Stem cells for dental tissue engineering

  1. 1. STEM CELLS FOR DENTAL TISSUE ENGINEERING By Sherly George MSc Biomedical Science Manchester Metropolitan University
  2. 2. Stem CellsStem cells:  Undifferentiated cells  Develop into differentiated cells with distinctive features and functions Ameloblasts Odontoblasts Enamel dentin Christopherson and Nesti Stem Cell Research & Therapy 2011 Dental tissue formation
  3. 3. Tooth anatomy www.vivianbaedds.com
  4. 4. TYPES OF DENTAL STEM CELLS • Dental Pulp Stem Cells (DPSCs) • Stem cells from human exfoliated deciduous teeth (SHEDs) • Periodontal Ligament (PDLSCs) • Dental follicle stem cells (DFSCs) • Apical papilla (SCAPs) Adapted from http://ww.bioscience.org.
  5. 5. Dental pulp stem cells • Multipotent cells • High proliferation rates • Accessibility • Generate dentin complex www.newsbrown.edu
  6. 6. Loss of tooth Images from: www.google.com dental caries periodotitis traumatic injury Pathological syndrome
  7. 7. Key elements of dental tissue engineering scaffold Stem cells Growth factors
  8. 8. Scaffolds • Cell attachment & migration • Permit delivery of growth factors • Enable influx of oxygen • Porosity is critical • Degradation is fundamental • Should not be toxic • Ceramics, natural or synthetic polymers
  9. 9. SCAFFOLDS collagen Hydroxyapatite 3Dhydrogel Porous ceramics
  10. 10. Growth factors (GF) • Soluble proteins • Signaling molecules for cells • Cell division, tissue differentiation • Regulate odontogenesis Bone morphogenic GF Transforming GF Fibroblast GF
  11. 11. Application of dental stem cells  Dental Pulp SC -Generate bone, dentin complex -Repair damaged dental tissues -Induce bone regeneration  Periodontal ligament SC -Form cementum & alveolar bone -Cures periodontal lesions in pig  Dental follicle SC -Forms cementum in vivo  SC from apical papilla -Osteoblasts & odondoblasts -Regenerative endodontic therapy
  12. 12. Advantage of dental stem cells • New promising therapeutic approach • Great potential for discovering new treatments & cure • Can regenerate a damaged tooth • Differentiate -connective, neural, muscle, bone & dental tissue • Repair bone damaged by disease or trauma
  13. 13. Disadvantage  More research required  DFSCs and SCAPs isolation: needs 3rd molar  Use of embryonic stem cells  Not ethical, not easily applicable  Expensive
  14. 14. REFERENCES • CAVENDER, A. C., APOS, ASOUZA, R. N., GALLER, K. M., KOEKLUE, U., SCHMALZ, G. & SUGGS, L. J. 2011. Bioengineering of dental stem cells in a PEGylated fibrin gel. Regenerative Medicine. • LYMPERI, S., LIGOUDISTIANOU, C., TARASLIA, V., KONTAKIOTIS, E. & ANASTASIADOU, E. 2013. Dental Stem Cells and their Applications in Dental Tissue Engineering. The open dentistry journal. • ROSA, V., DELLA BONA, A., CAVALCANTI, B. N. & NÖR, J. E. 2012. Tissue engineering: from research to dental clinics. Dental Materials, 28, 341-348. • MORAD, G., KHEIRI, L. & KHOJASTEH, A. 2013. Dental pulp stem cells for in vivo bone regeneration: A systematic review of literature. Archives of Oral Biology, 58, 1818-1827 • Barbara, Zavan, et al. "Dental Pulp Stem Cells and Tissue Engineering Strategies for Clinical Application on Odontoiatric Field." (2011). • Malhotra, Neeraj, and Kundabala Mala. "Regenerative endodontics as a tissue engineering approach: Past, current and future." Australian Endodontic Journal38.3 (2012): 137-148. http://www.sciencedirect.com/ http://www.ncbi.nlm.nih.gov/pubmed https://www.google.com/
  15. 15. Thank you

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