Stem cell research shows promise for treating Parkinson's disease. Studies have found that transplanting embryonic stem cells or neural stem cells into animal models of Parkinson's can reduce symptoms by generating new dopamine-producing cells. However, many of these transplanted cells have failed to maintain their function over time or have caused tumor formation. A clinical trial in Peru found that transplanting bone marrow-derived stem cells into the brains of Parkinson's patients improved their symptoms, but long-term data is still needed. Future research aims to develop safer and more effective stem cell therapies by improving protocols and understanding how to support long-term cell survival and specialization without tumor risks.
Stem cells
Undifferentiated cells capable of self-renew and to differentiate into different cell types or tissues during embryonic development and throughout adulthood.
Have possibility to become a specialised cell.
Have the ability to divide continuously and develop into various other kinds of cells.
Have immune potential and can help to treat a wide range of medical problems.
Discovery of stem cells lead to a whole new branch of medicine known as Regenerative medicine.
Scale up means increasing the quantity or volume of cell culture. For animal cells, the scale up strategies are dependent upon cell types or i.e. whether the cells requires matrix for attachment and growth ( adherent cell culture) or grows freely in suspended form in aqueous media. The scaling up principle for adherent cells are just to increase surface area for attachment while for suspension culture is to increase culture volume. This presentation enlightens the reader about different methods of scaling up of cells culture. Readers are also provided with sample questions for better understanding
Imagine that you have been told you have an illness that cannot be cured or what if your body has been irreversibly paralysed. There is no hope. But there is a science that could change that. It’s Called Stem Cell Research and it’s an important step in the medical revolution. But it comes with controversies as it uses Human Embryos’ as Raw Material.
But something astounding happened in the year 2006 that removed the usage of surplus embryos from the equation altogether. It’s about a brand new technology that can turn back the clock on your body cells. This is cutting edge of science where new developments are happing all the time. The iPSCs could be the potential medicine of 21st century. So what are stem cells? Why do they Matter? What are iPSCs and how it changed the biological rules?
German Scientist “Carl Vogt” was first to describe the principle of apoptosis in 1842. In 1885, Anatomist “Walther Flemming” gave more precise description of Programmed Cell Death. Apoptosis is a form of Programmed Cell Death that occurs in multicellular organisms. It is a Greek word which means falling off. It leads to breakdown and disposal of cells. Macrophages and other Phagocytic Cells remove them by Phagocytosis, without developing any type of inflammation. It is a biochemical event that leads to morphological changes and death. The average adult human looses 50-70 billion cells each day due to apoptosis.
Stem cells
Undifferentiated cells capable of self-renew and to differentiate into different cell types or tissues during embryonic development and throughout adulthood.
Have possibility to become a specialised cell.
Have the ability to divide continuously and develop into various other kinds of cells.
Have immune potential and can help to treat a wide range of medical problems.
Discovery of stem cells lead to a whole new branch of medicine known as Regenerative medicine.
Scale up means increasing the quantity or volume of cell culture. For animal cells, the scale up strategies are dependent upon cell types or i.e. whether the cells requires matrix for attachment and growth ( adherent cell culture) or grows freely in suspended form in aqueous media. The scaling up principle for adherent cells are just to increase surface area for attachment while for suspension culture is to increase culture volume. This presentation enlightens the reader about different methods of scaling up of cells culture. Readers are also provided with sample questions for better understanding
Imagine that you have been told you have an illness that cannot be cured or what if your body has been irreversibly paralysed. There is no hope. But there is a science that could change that. It’s Called Stem Cell Research and it’s an important step in the medical revolution. But it comes with controversies as it uses Human Embryos’ as Raw Material.
But something astounding happened in the year 2006 that removed the usage of surplus embryos from the equation altogether. It’s about a brand new technology that can turn back the clock on your body cells. This is cutting edge of science where new developments are happing all the time. The iPSCs could be the potential medicine of 21st century. So what are stem cells? Why do they Matter? What are iPSCs and how it changed the biological rules?
German Scientist “Carl Vogt” was first to describe the principle of apoptosis in 1842. In 1885, Anatomist “Walther Flemming” gave more precise description of Programmed Cell Death. Apoptosis is a form of Programmed Cell Death that occurs in multicellular organisms. It is a Greek word which means falling off. It leads to breakdown and disposal of cells. Macrophages and other Phagocytic Cells remove them by Phagocytosis, without developing any type of inflammation. It is a biochemical event that leads to morphological changes and death. The average adult human looses 50-70 billion cells each day due to apoptosis.
INTRODUCTION
ROLE IN CELL LINE CHARACTERIZATION
CAUSES OF TRANSFORMATION
METHODS OF TRANSFECTION
CHARACTERISTICS OF TRAANSFORMED CELLS
GENETIC INSTABILITY
IMMORTALIZATION
ABRERANT GROWTH CONTROL
TUMORIGENECITY
CHROMOSOMAL ABERATION
APPLICATION
CONCLUSION
REFERENCE
Role of serum and supplements in culture medium k.skailash saini
ROLE OF SERUM AND SUPPLEMENTS IN CULTURE MEDIA
Serum is a complex mix of albumins, growth factors and growth inhibitors.
Serum is one of the most important components of cell culture media and serves as a source for amino acids, proteins, vitamins (particularly fat-soluble vitamins such as A, D, E, and K), carbohydrates, lipids, hormones, growth factors, minerals, and trace elements.
Serum from fetal and calf bovine sources are commonly used to support the growth of cells in culture.
Fetal serum is a rich source of growth factors and is appropriate for cell cloning and for the growth of fastidious cells.
Calf serum is used in contact-inhibition studies because of its lower growth-promoting properties.
Normal growth media often contain 2-10% of serum.
Supplementation of media with serum serves the following functions :
Serum provides the basic nutrients (both in the solution as well as bound to the proteins) for cells.
Serum provides several growth factors and hormones involved in growth promotion and specialized cell function.
It provides several binding proteins like albumin, transferrin, which can carry other molecules into the cell. For example: albumin carries lipids, vitamins, hormones, etc. into cells.
It also supplies proteins, like fibronectin, which promote the attachment of cells to the substrate. It also provides spreading factors that help the cells to spread out before they begin to divide.
It provides protease inhibitors which protect cells from proteolysis.
It also provides minerals, like Na+, K+, Zn2+, Fe2+, etc.
It increases the viscosity of the medium and thus, protects cells from mechanical damages during agitation of suspension cultures.
It also acts a buffer.
Due to the presence of both growth factors and inhibitors, the role of serum in cell culture is very complex.
Unfortunately, in addition to serving various functions, the use of serum in tissue culture applications has several drawbacks .
Blood production agency. all types of blood cellls are produced in it. to understand it is the need of this era. it also will help in the physiology of blood making mechanism.
Introduction
History
Cell culture techniques
Species cloned
Approaches of cell cloning
Monolayer culture- Dilution cloning
Microtitration plate
Suspension culture- Cloning in agar
Cloning in methocel
Isolation of clone
By clonal rings
By suspension clone
Application of cell cloning
Conclusion
Reference
INTRODUCTION
ROLE IN CELL LINE CHARACTERIZATION
CAUSES OF TRANSFORMATION
METHODS OF TRANSFECTION
CHARACTERISTICS OF TRAANSFORMED CELLS
GENETIC INSTABILITY
IMMORTALIZATION
ABRERANT GROWTH CONTROL
TUMORIGENECITY
CHROMOSOMAL ABERATION
APPLICATION
CONCLUSION
REFERENCE
Role of serum and supplements in culture medium k.skailash saini
ROLE OF SERUM AND SUPPLEMENTS IN CULTURE MEDIA
Serum is a complex mix of albumins, growth factors and growth inhibitors.
Serum is one of the most important components of cell culture media and serves as a source for amino acids, proteins, vitamins (particularly fat-soluble vitamins such as A, D, E, and K), carbohydrates, lipids, hormones, growth factors, minerals, and trace elements.
Serum from fetal and calf bovine sources are commonly used to support the growth of cells in culture.
Fetal serum is a rich source of growth factors and is appropriate for cell cloning and for the growth of fastidious cells.
Calf serum is used in contact-inhibition studies because of its lower growth-promoting properties.
Normal growth media often contain 2-10% of serum.
Supplementation of media with serum serves the following functions :
Serum provides the basic nutrients (both in the solution as well as bound to the proteins) for cells.
Serum provides several growth factors and hormones involved in growth promotion and specialized cell function.
It provides several binding proteins like albumin, transferrin, which can carry other molecules into the cell. For example: albumin carries lipids, vitamins, hormones, etc. into cells.
It also supplies proteins, like fibronectin, which promote the attachment of cells to the substrate. It also provides spreading factors that help the cells to spread out before they begin to divide.
It provides protease inhibitors which protect cells from proteolysis.
It also provides minerals, like Na+, K+, Zn2+, Fe2+, etc.
It increases the viscosity of the medium and thus, protects cells from mechanical damages during agitation of suspension cultures.
It also acts a buffer.
Due to the presence of both growth factors and inhibitors, the role of serum in cell culture is very complex.
Unfortunately, in addition to serving various functions, the use of serum in tissue culture applications has several drawbacks .
Blood production agency. all types of blood cellls are produced in it. to understand it is the need of this era. it also will help in the physiology of blood making mechanism.
Introduction
History
Cell culture techniques
Species cloned
Approaches of cell cloning
Monolayer culture- Dilution cloning
Microtitration plate
Suspension culture- Cloning in agar
Cloning in methocel
Isolation of clone
By clonal rings
By suspension clone
Application of cell cloning
Conclusion
Reference
Dr. David Greene Arizona Stem Cell Therapy to Treat Parkinson's Disease.pdfDr. David Greene Arizona
A neurological condition called Parkinson's disease is characterized by increasingly severe pain. It affects about 1% of seniors 60 years or older in industrialized nations. Reliable Source Experts like Dr. David Greene Arizona believe that inherited and environmental factors may be involved, even if the exact cause of the condition remains unknown. Dopamine levels decrease due to Parkinson's disease-related neuronal death in particular brain areas. Dopamine is a neurotransmitter. Brain cells emit dopamine to communicate with other nearby cells.
Feature story from the Garvan Institute of Medical Research's August 2012 issue of Breakthrough newsletter. More at https://www.garvan.org.au/news-events/newsletters
IN-VIVO SCREENING METHODS FOR NEURODEGENERATIVE DISEASE.pptxGautamSosa
Neurodegenerative diseases are conditions where nerve cells in the brain and peripheral nervous system gradually degenerate and die, leading to cognitive decline, movement disorders, and sometimes death. Examples include Alzheimer's, Parkinson's, multiple sclerosis and Huntington's disease. Treatment focuses on symptom management, as there are currently no cures for these conditions. Efficacy evaluation of any drug for Alzheimer's, Parkinson's, and multiple sclerosis in in-vivo animal studies, first step is to learn the in-vivo screening model of particular disease.
Pain becomes increasingly severe in Parkinson's disease, a neurological condition. It affects about 1% of seniors 60 years or older in industrialized nations. Reliable Source Experts like Dr. David Greene Arizona believe that inherited and environmental factors may be involved, even if the exact cause of the condition remains unknown. Dopamine levels decrease due to Parkinson's disease-related neuronal death in particular brain areas. For more information, visit our website.
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This is my report on our cell biology. I hope it could help you.
Objectives: Identify infectious proteins (PrPsc), difference of PrPc and PrPsc, list of neurodegenerative diseases that caused by prions.
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Flu Vaccine Alert in Bangalore Karnatakaaddon Scans
As flu season approaches, health officials in Bangalore, Karnataka, are urging residents to get their flu vaccinations. The seasonal flu, while common, can lead to severe health complications, particularly for vulnerable populations such as young children, the elderly, and those with underlying health conditions.
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Basavarajeeyam is an important text for ayurvedic physician belonging to andhra pradehs. It is a popular compendium in various parts of our country as well as in andhra pradesh. The content of the text was presented in sanskrit and telugu language (Bilingual). One of the most famous book in ayurvedic pharmaceutics and therapeutics. This book contains 25 chapters called as prakaranas. Many rasaoushadis were explained, pioneer of dhatu druti, nadi pareeksha, mutra pareeksha etc. Belongs to the period of 15-16 century. New diseases like upadamsha, phiranga rogas are explained.
1. Possible Future Treatment for Parkinson’sPossible Future Treatment for Parkinson’s
disease?disease?
Ms Veena Shriram.
Lecturer,
Dept of Physiology,
B.J. Medical College, Pune -1
2. Parkinson’s Disease (PD)Parkinson’s Disease (PD)
First description 1817First description 1817
Parkinson, James An Essay on the Shaking Palsy.Parkinson, James An Essay on the Shaking Palsy.
Progressive neurodegenerative diseaseProgressive neurodegenerative disease
Affects ages 40 onwards, mean age atAffects ages 40 onwards, mean age at
diagnosis 70.5diagnosis 70.5
Complex disorder with motor, non-motor,Complex disorder with motor, non-motor,
neuropsychiatric featuresneuropsychiatric features
3. When most people reach for a pen, their body acts in one smooth andWhen most people reach for a pen, their body acts in one smooth and
controlled movement. The instant a person thinks of grabbing the pen,controlled movement. The instant a person thinks of grabbing the pen,
a series of nerve cells fire in an orchestrated symphony from the braina series of nerve cells fire in an orchestrated symphony from the brain
to the muscles responsible for that action.to the muscles responsible for that action.
For the movement to be precise and smooth, all the nerve cells in theFor the movement to be precise and smooth, all the nerve cells in the
“grabbing-the-pen network” must function properly, including cells“grabbing-the-pen network” must function properly, including cells
that tell unneeded muscles to stay still.that tell unneeded muscles to stay still.
In Parkinson’s disease (P. D.) the brain cells responsible for keepingIn Parkinson’s disease (P. D.) the brain cells responsible for keeping
unneeded muscles from movingunneeded muscles from moving degeneratedegenerate andand diedie..
That is in P. D. nerve cells that make the chemicalThat is in P. D. nerve cells that make the chemical dopaminedopamine die. Thisdie. This
results in progressively more dramatic and uncontrolled movements,results in progressively more dramatic and uncontrolled movements,
tremors, and spasms.tremors, and spasms.
To date, there is no cure for P. D. because no one has figured out a wayTo date, there is no cure for P. D. because no one has figured out a way
to bring back the specialized nerve cells that have died.to bring back the specialized nerve cells that have died.
4. Parkinson’s Disease (PD)Parkinson’s Disease (PD)
Parkinson's disease is a disorder of the brain
characterized by shaking (tremor) and difficulty
with walking, movement, and coordination. The
disease is associated with damage to a part of
the brain that is involved with movement.
5. Causes of PDCauses of PD
Parkinson‘s disease is caused by progressive deterioration of
the nerve cells of the part of the brain that controls muscle
movement. Dopamine, which is one of the substances used by
cells to transmit impulses, is normally produced in this area.
Deterioration of this area of the brain reduces the amount of
dopamine available to the body.
Insufficient dopamine disturbs the balance between dopamine
and other transmitters, such as acetylcholine. Without
dopamine, the nerve cells cannot properly transmit messages,
and this results in the loss of muscle function.
7. Healthy balance of dopamine
and acetylcholine
Imbalance of dopamine and
acetylcholine in Parkinson's
disease.
8. Signs and symptoms of Parkinson's diseaseSigns and symptoms of Parkinson's disease
Characterized by: (Characterized by: (SSlow,low, SStiff,tiff, SShaky)haky)
Rest tremor--3-6Hz pill-rolling.Rest tremor--3-6Hz pill-rolling.
Slowed motion (bradykinesia).
Rigidity of muscles
Loss of automatic movements
Impaired speech
Difficulty swallowing
Dementia
Postural instabilityPostural instability
9. 2002 Researchers injected Parkinson’s rats with mouse2002 Researchers injected Parkinson’s rats with mouse
embryonic stem cells. The rats showed a modest benefit for justembryonic stem cells. The rats showed a modest benefit for just
over 50% of the rats, but one-fifth (20%) of the rats died of brainover 50% of the rats, but one-fifth (20%) of the rats died of brain
tumors caused by the embryonic stem cells.tumors caused by the embryonic stem cells.
Bjorklund LM et al.,Bjorklund LM et al., Embryonic stem cells develop into functionalEmbryonic stem cells develop into functional
dopaminergic neurons after transplantation in a Parkinson rat model.dopaminergic neurons after transplantation in a Parkinson rat model.
Proc. Natl. Acad. Sci. 99, 2344-2349, February 19, 2002.Proc. Natl. Acad. Sci. 99, 2344-2349, February 19, 2002.
20032003 DopaminergicDopaminergic neurons made from mouse embryonic stemneurons made from mouse embryonic stem
cells were transplanted into Parkinson's mice and provided somecells were transplanted into Parkinson's mice and provided some
decrease in symptoms, but 20% of mice receiving the embryonicdecrease in symptoms, but 20% of mice receiving the embryonic
stem cells died due to teratoma formation.stem cells died due to teratoma formation.
F Nishimura et al.,F Nishimura et al., Potential use of embryonic stem cells for thePotential use of embryonic stem cells for the
treatment of mouse Parkinsonian models: improved behavior bytreatment of mouse Parkinsonian models: improved behavior by
transplantation of in vitro differentiated dopaminergic neurons fromtransplantation of in vitro differentiated dopaminergic neurons from
embryonic stem cells.embryonic stem cells. Stem Cells 21, 171-180; March 2003.Stem Cells 21, 171-180; March 2003.
10. 2004 An Israeli team turned human embryonic stem cells (hES)2004 An Israeli team turned human embryonic stem cells (hES)
into neural progenitors and transplanted these into rats. Someinto neural progenitors and transplanted these into rats. Some
cells made dopamine, but the cells stopped growing at 12cells made dopamine, but the cells stopped growing at 12
weeks. The rats exhibited a partial improvement in behavioralweeks. The rats exhibited a partial improvement in behavioral
tests, but it was too early to see if tumors formed.tests, but it was too early to see if tumors formed.
Ben-Hur T, et al.,Ben-Hur T, et al., Transplantation of human embryonic stem cell-Transplantation of human embryonic stem cell-
derived neural progenitors improves behavioral deficit in Parkinsonsderived neural progenitors improves behavioral deficit in Parkinsons
rats.rats. Stem Cells 22 (7): 1246-55, 2004.Stem Cells 22 (7): 1246-55, 2004.
2005 A Japanese team turned monkey embryonic stem cells2005 A Japanese team turned monkey embryonic stem cells
into neural stem cells. They transplanted these into monkeysinto neural stem cells. They transplanted these into monkeys
with artificially induced Parkinsons’s, and some cells turnedwith artificially induced Parkinsons’s, and some cells turned
into dopamine producing cells. There was mild alleviation ofinto dopamine producing cells. There was mild alleviation of
symptoms.symptoms. Yasushi Takagi et al.,Yasushi Takagi et al., Dopaminergic neurons generatedDopaminergic neurons generated
from monkey embryonic stem cells function in a Parkinson primatefrom monkey embryonic stem cells function in a Parkinson primate
model.model.
11. • 2006 Scientists in Sweden and Japan found no improvement of
Parkinson’s rats treated with embryonic stem cells, and many
animals developed severe tumors.
Brederlau A, et al., Transplantation of human embryonic stem cell-derived
cells to a rat model of parkinson’s disease: effect of in vitro differentiation
on graft survival and teratoma formation. Stem Cells express online
publication doi:10.1634/stemcells.2005-0393, March 23, 2006.
• 2006 Researchers turned embryonic stem cells into dopamine
producing cells, and when injected into rats with a Parkinson’s-like
condition, the rats showed improvement. However, in 100% of rats
the cells began to lose their specialization and grow uncontrollably.
All the animals showed indications of early tumor formation.
Roy N et al., Functional engraftment of human ES cell–derived
dopaminergic neurons enriched by coculture with telomerase-
immortalized midbrain astrocytes. Nature Medicine 12, 1259-68;
November 2006.
12. A study by Instituto Brazzini Radiologos Asociados in Lima,A study by Instituto Brazzini Radiologos Asociados in Lima,
Peru, showed considerable improvement in Parkinson'sPeru, showed considerable improvement in Parkinson's
symptoms after stem cell implants. Doctors registered varioussymptoms after stem cell implants. Doctors registered various
degrees of beneficial changes in brains of all 47 patients withindegrees of beneficial changes in brains of all 47 patients within
one week of the treatment. A team of Dr. Augusto Brazzinione week of the treatment. A team of Dr. Augusto Brazzini
Armestar, the director of the institute, infusedArmestar, the director of the institute, infused autologous stemautologous stem
cells derived from bone marrowcells derived from bone marrow into the arteries that supplyinto the arteries that supply
blood to parts of brain that are typically damaged byblood to parts of brain that are typically damaged by
Parkinson's disease. The findings show a clinical recovery ofParkinson's disease. The findings show a clinical recovery of
extrapyramidal symptoms, which are maintained over time, asextrapyramidal symptoms, which are maintained over time, as
well as function recovery, representing a better metabolism ofwell as function recovery, representing a better metabolism of
neurons and better performance in the brain.neurons and better performance in the brain.
Susman, Ed, "Stem Cell Implant to the Brain Helps ImproveSusman, Ed, "Stem Cell Implant to the Brain Helps Improve
Parkinson's Symptoms:Parkinson's Symptoms: Accessed at: ( 17 April 2008).Accessed at: ( 17 April 2008).
http://www.docguide.com/news/content.nsf/news/852571020057CCF6852574http://www.docguide.com/news/content.nsf/news/852571020057CCF6852574
1600511A241600511A24 <http://www.stemcellresearchfacts.org/parkinsons-disease/><http://www.stemcellresearchfacts.org/parkinsons-disease/>
13. Stem cells were taken from patients' bones and were sent to theStem cells were taken from patients' bones and were sent to the
laboratory to be separated and purified. A team oflaboratory to be separated and purified. A team of
interventionists advanced a catheter from an incision into theinterventionists advanced a catheter from an incision into the
groin that gained access to the arterial system. From there, undergroin that gained access to the arterial system. From there, under
imaging guidance, the catheter was advanced through the carotidimaging guidance, the catheter was advanced through the carotid
artery, the posterior cerebral arteries, and the posteriorartery, the posterior cerebral arteries, and the posterior
communicating arteries. At that time, the stem cells were slowlycommunicating arteries. At that time, the stem cells were slowly
infused through the catheter into the arteries that irrigate theinfused through the catheter into the arteries that irrigate the
basal nucleus and the substantia nigra -- an area where neuronsbasal nucleus and the substantia nigra -- an area where neurons
are depleted in Parkinson's patients.are depleted in Parkinson's patients.
To date, the team has implanted stem cells in 15 women and 30To date, the team has implanted stem cells in 15 women and 30
men. The average age of the patients was about 50 years;men. The average age of the patients was about 50 years;
Parkinson's disease had been diagnosed from 1 year to 18 yearsParkinson's disease had been diagnosed from 1 year to 18 years
before implantation. At the 1-week follow-up, 39 patients hadbefore implantation. At the 1-week follow-up, 39 patients had
achieved a 35% improvement as assessed by a battery ofachieved a 35% improvement as assessed by a battery of
Parkinson's disease validated tests. At the 1-month follow-up, 34Parkinson's disease validated tests. At the 1-month follow-up, 34
patients showed a 52% improvement. At 3 months, 23 patientspatients showed a 52% improvement. At 3 months, 23 patients
had improved an average of 59%; at 6 months, 6 patients hadhad improved an average of 59%; at 6 months, 6 patients had
improved 76%; after 12 months the 1 person to reach that levelimproved 76%; after 12 months the 1 person to reach that level
had achieved an 80% improvement (had achieved an 80% improvement (P P < .001).< .001).
14. • Pharmacological therapies are valuable but suffer from two
main drawbacks: side effects and loss of efficacy with disease
progression.
• Surgical treatment is no better than drugs.
• Transplantation of embryonic mesencephalic tissue has
emerged as a therapeutic alternative, but the unstable efficiency
and the shortage of embryonic donors limit its clinical
application.
• Recent advances in stem cell research inspire our hope that
stem cell transplantation to replace degenerated neurons may
be a promising therapy for Parkinson’s disease.
• There are three sources of stem cells currently in testing:
embryonic stem cells, neural stem cells, and mesenchymal stem
cells. The stem cell transplantation in the animal model of
Parkinson’s disease proves that it is capable of relieving
symptoms and restoring damaged brain function.
15. Stem cell research seems to be promising in regeneratingStem cell research seems to be promising in regenerating
hope to cure Parkinson’s disease. This will motivatehope to cure Parkinson’s disease. This will motivate
innumerable patients across the world to explore this newinnumerable patients across the world to explore this new
modality. However we need to observe the long termmodality. However we need to observe the long term
clinical effects in large number of patients to decide its roleclinical effects in large number of patients to decide its role
in the treatment of the degenerative diseases.in the treatment of the degenerative diseases.
Future stem cell research should focus not only onFuture stem cell research should focus not only on
improving the symptoms of P.D. but also onimproving the symptoms of P.D. but also on
neuroprotection that can favourably modify natural courseneuroprotection that can favourably modify natural course
and slow progression of the disease.and slow progression of the disease.
16. To make these therapies more accessible and effective itTo make these therapies more accessible and effective it
will be important to improve clinical protocols and gene-will be important to improve clinical protocols and gene-
delivery vectors, and to gain a deeper understanding ofdelivery vectors, and to gain a deeper understanding of
stem cells.stem cells.
The present challenge is to reduce the risk of suchThe present challenge is to reduce the risk of such
transplants and increase the number of patients who cantransplants and increase the number of patients who can
safely access this treatment. In developing countries,safely access this treatment. In developing countries,
such ‘one-shot’ treatments are highly desirable becausesuch ‘one-shot’ treatments are highly desirable because
chronic treatments are difficult to sustain.chronic treatments are difficult to sustain.
17. What happens next?
What everyone is anxious to know is, "What will happen
next in Parkinson's disease research?" Two critical goals are
to develop diagnostic approaches that allow early recognition
of PD and to find a way to slow the disease's progression.
The development of effective and safe cell therapies is of
critical importance, and scientists have multiple avenues to
explore that require intensive research.
A major challenge researchers face is getting the
transplanted DA embryonic stem cells to maintain their
new form in large numbers and over an extended period
of time without forming tumours.
19. AcknowledgementAcknowledgement
Dr. (Mrs.) S. M. Vaidya,Dr. (Mrs.) S. M. Vaidya,
Prof. & Head*Prof. & Head*
Dr.(Mrs.) N.V. Aundhkar,Dr.(Mrs.) N.V. Aundhkar,
Professor *Professor *
* Dept of Physiology,* Dept of Physiology,
B. J. Medical College, PuneB. J. Medical College, Pune
20.
21. • Current state of stem cell research for the treatment of Parkinson’s disease.
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