The document discusses induced pluripotent stem cells (iPSCs), which are derived from adult somatic cells that are reprogrammed by introducing genes associated with pluripotency. iPSCs were first generated in 2006 and resemble embryonic stem cells. They can be produced from a person's own cells and have potential applications in disease modeling, drug development, and regenerative medicine without ethical issues associated with embryonic stem cells.
Imagine that you have been told you have an illness that cannot be cured or what if your body has been irreversibly paralysed. There is no hope. But there is a science that could change that. It’s Called Stem Cell Research and it’s an important step in the medical revolution. But it comes with controversies as it uses Human Embryos’ as Raw Material.
But something astounding happened in the year 2006 that removed the usage of surplus embryos from the equation altogether. It’s about a brand new technology that can turn back the clock on your body cells. This is cutting edge of science where new developments are happing all the time. The iPSCs could be the potential medicine of 21st century. So what are stem cells? Why do they Matter? What are iPSCs and how it changed the biological rules?
iPSCs are pluripotent; unlike ESC, iPSCs are not derived from the embryo, but instead created from differentiated cells in the lab through a process – cellular reprogramming.
Stem cells
Undifferentiated cells capable of self-renew and to differentiate into different cell types or tissues during embryonic development and throughout adulthood.
Have possibility to become a specialised cell.
Have the ability to divide continuously and develop into various other kinds of cells.
Have immune potential and can help to treat a wide range of medical problems.
Discovery of stem cells lead to a whole new branch of medicine known as Regenerative medicine.
Imagine that you have been told you have an illness that cannot be cured or what if your body has been irreversibly paralysed. There is no hope. But there is a science that could change that. It’s Called Stem Cell Research and it’s an important step in the medical revolution. But it comes with controversies as it uses Human Embryos’ as Raw Material.
But something astounding happened in the year 2006 that removed the usage of surplus embryos from the equation altogether. It’s about a brand new technology that can turn back the clock on your body cells. This is cutting edge of science where new developments are happing all the time. The iPSCs could be the potential medicine of 21st century. So what are stem cells? Why do they Matter? What are iPSCs and how it changed the biological rules?
iPSCs are pluripotent; unlike ESC, iPSCs are not derived from the embryo, but instead created from differentiated cells in the lab through a process – cellular reprogramming.
Stem cells
Undifferentiated cells capable of self-renew and to differentiate into different cell types or tissues during embryonic development and throughout adulthood.
Have possibility to become a specialised cell.
Have the ability to divide continuously and develop into various other kinds of cells.
Have immune potential and can help to treat a wide range of medical problems.
Discovery of stem cells lead to a whole new branch of medicine known as Regenerative medicine.
A stem cell is a "blank" cell that can give rise to multiple tissue types such as a skin, muscle, or nerve cell.
Under certain physiologic or experimental conditions, they can be induced to become tissue- or organ-specific cells with special functions.
PRODUCTION AND MAINTENANCE OF EMBRYONIC STEM CELLSANKUR SHARMA
Embryonic stem cells are pluripotent stem cells and have capacity to differentiate into all type of cells arising from 3 different germ layers i.e., ecto-, meso- and endoderm. In this presentation brief information is given about different methods for production of embryonic stem cells and their maintenance
INTRODUCTION
HISTORY
NEED OF SYNCHRONIZATION
SYNCHRONOUS CULTURES CAN BE OBTAINED IN SEVERAL WAYS:
Physical fractionation .
Chemical appro ach
CENTRIFUGAL ELUTRIATION
Inhibition of DNA synthesis
Nutritional deprivation
SYNCHRONIZATION AT LOW TEMPERATURE
CELLULAR TOTIPOTENCY
SOME HIGHLIGHTS OF CELL SYNCHRONIZATION
REFERENCES
Stem cells are the cells which have the capability to differentiate into any cells of the body when provided with right stimulus and environment. This presentation teaches about stem cells, characteristics, types and cultivation of stem cells in artificial environment. Sample practice questions are also provided in the end to review the concept learned from this presentation.
PowerPoint giving a summary on research in stem cells (brief historical overview), and the explanatory component of the papers which changed the game of stem cell research Yamanka's Nuclear Reprogramming.
it gives detail or you can say brief introduction of iPS cells , what are they , how can be obtained , what are the future possibilities of iPS cells what promise it made to upcoming future technology to medical health
A stem cell is a "blank" cell that can give rise to multiple tissue types such as a skin, muscle, or nerve cell.
Under certain physiologic or experimental conditions, they can be induced to become tissue- or organ-specific cells with special functions.
PRODUCTION AND MAINTENANCE OF EMBRYONIC STEM CELLSANKUR SHARMA
Embryonic stem cells are pluripotent stem cells and have capacity to differentiate into all type of cells arising from 3 different germ layers i.e., ecto-, meso- and endoderm. In this presentation brief information is given about different methods for production of embryonic stem cells and their maintenance
INTRODUCTION
HISTORY
NEED OF SYNCHRONIZATION
SYNCHRONOUS CULTURES CAN BE OBTAINED IN SEVERAL WAYS:
Physical fractionation .
Chemical appro ach
CENTRIFUGAL ELUTRIATION
Inhibition of DNA synthesis
Nutritional deprivation
SYNCHRONIZATION AT LOW TEMPERATURE
CELLULAR TOTIPOTENCY
SOME HIGHLIGHTS OF CELL SYNCHRONIZATION
REFERENCES
Stem cells are the cells which have the capability to differentiate into any cells of the body when provided with right stimulus and environment. This presentation teaches about stem cells, characteristics, types and cultivation of stem cells in artificial environment. Sample practice questions are also provided in the end to review the concept learned from this presentation.
PowerPoint giving a summary on research in stem cells (brief historical overview), and the explanatory component of the papers which changed the game of stem cell research Yamanka's Nuclear Reprogramming.
it gives detail or you can say brief introduction of iPS cells , what are they , how can be obtained , what are the future possibilities of iPS cells what promise it made to upcoming future technology to medical health
Reprogramming to pluripotency is possible from adult cells of different tissues and species through the ectopic expression of defined factors. The generated induced Pluripotent Stem Cells (iPSCs) are relevant for various purposes, including disease modeling, drug or toxicity screening and autologous cell therapy. Over the last few years, increased efforts are being made to improve the reprogramming techniques, the efficiency and quality of the generated iPSCs, as well as to identify the best cell source to be reprogrammed. Cells derived from fetal tissues, such as amniotic fluid, placenta and umbilical cord, offer distinct advantages in terms of reprogramming compared to adult somatic cells. Importantly, fetal cells are more primitive, easily achievable in sufficient numbers and are devoid of any ethical concern. They show great plasticity, high proliferation rate, low immunogenity and absence of teratoma formation. Therefore, they can be reprogrammed much faster and more efficiently than adult cells. Here, we provide a comprehensive overview of the advantages of reprogramming fetal sources in comparison to other commonly used cell types.
Stem Cell Technology and its Clinical ApplicationDr. Barkha Gupta
Dr. Barkha Gupta has been teaching Veterinary Biochemistry as well as clinical physiology at CVAS, Udaipur and PGIVER, Jaipur. She has earlier served in various capacities in the Department of Animal Husbandry, Govt. of Rajasthan. She has several publications and awards to her credit. She is the PI of M-RAJUVAS Android Educational Mobile Application for Veterinary and Animal Sciences and Kiosk Information System for Farmers/Livestock Owners. Dr. Gupta is also IFBA Certified Professional.
Induced Pluripotent Stem-Like Cells Derived from Ban, a Vietnamese Native Pig...AI Publications
Induced pluripotent stem cells (iPSc) is a promising technology for applying in bio-medicine and biodiversity conservation. In the present study, we isolate and culture fibroblasts from Ban – a Vietnamese native pig breed and transfer episomal plasmid containing genes Oct3/4, Sox2, Klf4, l-Myc, LIN28 and EBNA1 in order to reprogram cells. We isolated, cultured and cryopreserved successfully 9 primary fibroblast lines from Ban (culture percentage is 90.0%). Plasmids was successfully transferred into Ban fibroblasts with high efficiency. Changes in morphology of fibroblasts into pluripotent stem-like cells showed that they had been reprogrammed under the effect of transferred genes. The pluripotency signal was further proved by in vitro differentiation by formation of embryoid body in all 3 transfected cell lines. The results showed that pluripotent stem-like cells has successfully derived in Ban pigs.
Transcript: Selling digital books in 2024: Insights from industry leaders - T...BookNet Canada
The publishing industry has been selling digital audiobooks and ebooks for over a decade and has found its groove. What’s changed? What has stayed the same? Where do we go from here? Join a group of leading sales peers from across the industry for a conversation about the lessons learned since the popularization of digital books, best practices, digital book supply chain management, and more.
Link to video recording: https://bnctechforum.ca/sessions/selling-digital-books-in-2024-insights-from-industry-leaders/
Presented by BookNet Canada on May 28, 2024, with support from the Department of Canadian Heritage.
Accelerate your Kubernetes clusters with Varnish CachingThijs Feryn
A presentation about the usage and availability of Varnish on Kubernetes. This talk explores the capabilities of Varnish caching and shows how to use the Varnish Helm chart to deploy it to Kubernetes.
This presentation was delivered at K8SUG Singapore. See https://feryn.eu/presentations/accelerate-your-kubernetes-clusters-with-varnish-caching-k8sug-singapore-28-2024 for more details.
Let's dive deeper into the world of ODC! Ricardo Alves (OutSystems) will join us to tell all about the new Data Fabric. After that, Sezen de Bruijn (OutSystems) will get into the details on how to best design a sturdy architecture within ODC.
Search and Society: Reimagining Information Access for Radical FuturesBhaskar Mitra
The field of Information retrieval (IR) is currently undergoing a transformative shift, at least partly due to the emerging applications of generative AI to information access. In this talk, we will deliberate on the sociotechnical implications of generative AI for information access. We will argue that there is both a critical necessity and an exciting opportunity for the IR community to re-center our research agendas on societal needs while dismantling the artificial separation between the work on fairness, accountability, transparency, and ethics in IR and the rest of IR research. Instead of adopting a reactionary strategy of trying to mitigate potential social harms from emerging technologies, the community should aim to proactively set the research agenda for the kinds of systems we should build inspired by diverse explicitly stated sociotechnical imaginaries. The sociotechnical imaginaries that underpin the design and development of information access technologies needs to be explicitly articulated, and we need to develop theories of change in context of these diverse perspectives. Our guiding future imaginaries must be informed by other academic fields, such as democratic theory and critical theory, and should be co-developed with social science scholars, legal scholars, civil rights and social justice activists, and artists, among others.
PHP Frameworks: I want to break free (IPC Berlin 2024)Ralf Eggert
In this presentation, we examine the challenges and limitations of relying too heavily on PHP frameworks in web development. We discuss the history of PHP and its frameworks to understand how this dependence has evolved. The focus will be on providing concrete tips and strategies to reduce reliance on these frameworks, based on real-world examples and practical considerations. The goal is to equip developers with the skills and knowledge to create more flexible and future-proof web applications. We'll explore the importance of maintaining autonomy in a rapidly changing tech landscape and how to make informed decisions in PHP development.
This talk is aimed at encouraging a more independent approach to using PHP frameworks, moving towards a more flexible and future-proof approach to PHP development.
Kubernetes & AI - Beauty and the Beast !?! @KCD Istanbul 2024Tobias Schneck
As AI technology is pushing into IT I was wondering myself, as an “infrastructure container kubernetes guy”, how get this fancy AI technology get managed from an infrastructure operational view? Is it possible to apply our lovely cloud native principals as well? What benefit’s both technologies could bring to each other?
Let me take this questions and provide you a short journey through existing deployment models and use cases for AI software. On practical examples, we discuss what cloud/on-premise strategy we may need for applying it to our own infrastructure to get it to work from an enterprise perspective. I want to give an overview about infrastructure requirements and technologies, what could be beneficial or limiting your AI use cases in an enterprise environment. An interactive Demo will give you some insides, what approaches I got already working for real.
Connector Corner: Automate dynamic content and events by pushing a buttonDianaGray10
Here is something new! In our next Connector Corner webinar, we will demonstrate how you can use a single workflow to:
Create a campaign using Mailchimp with merge tags/fields
Send an interactive Slack channel message (using buttons)
Have the message received by managers and peers along with a test email for review
But there’s more:
In a second workflow supporting the same use case, you’ll see:
Your campaign sent to target colleagues for approval
If the “Approve” button is clicked, a Jira/Zendesk ticket is created for the marketing design team
But—if the “Reject” button is pushed, colleagues will be alerted via Slack message
Join us to learn more about this new, human-in-the-loop capability, brought to you by Integration Service connectors.
And...
Speakers:
Akshay Agnihotri, Product Manager
Charlie Greenberg, Host
GDG Cloud Southlake #33: Boule & Rebala: Effective AppSec in SDLC using Deplo...James Anderson
Effective Application Security in Software Delivery lifecycle using Deployment Firewall and DBOM
The modern software delivery process (or the CI/CD process) includes many tools, distributed teams, open-source code, and cloud platforms. Constant focus on speed to release software to market, along with the traditional slow and manual security checks has caused gaps in continuous security as an important piece in the software supply chain. Today organizations feel more susceptible to external and internal cyber threats due to the vast attack surface in their applications supply chain and the lack of end-to-end governance and risk management.
The software team must secure its software delivery process to avoid vulnerability and security breaches. This needs to be achieved with existing tool chains and without extensive rework of the delivery processes. This talk will present strategies and techniques for providing visibility into the true risk of the existing vulnerabilities, preventing the introduction of security issues in the software, resolving vulnerabilities in production environments quickly, and capturing the deployment bill of materials (DBOM).
Speakers:
Bob Boule
Robert Boule is a technology enthusiast with PASSION for technology and making things work along with a knack for helping others understand how things work. He comes with around 20 years of solution engineering experience in application security, software continuous delivery, and SaaS platforms. He is known for his dynamic presentations in CI/CD and application security integrated in software delivery lifecycle.
Gopinath Rebala
Gopinath Rebala is the CTO of OpsMx, where he has overall responsibility for the machine learning and data processing architectures for Secure Software Delivery. Gopi also has a strong connection with our customers, leading design and architecture for strategic implementations. Gopi is a frequent speaker and well-known leader in continuous delivery and integrating security into software delivery.
GraphRAG is All You need? LLM & Knowledge GraphGuy Korland
Guy Korland, CEO and Co-founder of FalkorDB, will review two articles on the integration of language models with knowledge graphs.
1. Unifying Large Language Models and Knowledge Graphs: A Roadmap.
https://arxiv.org/abs/2306.08302
2. Microsoft Research's GraphRAG paper and a review paper on various uses of knowledge graphs:
https://www.microsoft.com/en-us/research/blog/graphrag-unlocking-llm-discovery-on-narrative-private-data/
Epistemic Interaction - tuning interfaces to provide information for AI supportAlan Dix
Paper presented at SYNERGY workshop at AVI 2024, Genoa, Italy. 3rd June 2024
https://alandix.com/academic/papers/synergy2024-epistemic/
As machine learning integrates deeper into human-computer interactions, the concept of epistemic interaction emerges, aiming to refine these interactions to enhance system adaptability. This approach encourages minor, intentional adjustments in user behaviour to enrich the data available for system learning. This paper introduces epistemic interaction within the context of human-system communication, illustrating how deliberate interaction design can improve system understanding and adaptation. Through concrete examples, we demonstrate the potential of epistemic interaction to significantly advance human-computer interaction by leveraging intuitive human communication strategies to inform system design and functionality, offering a novel pathway for enriching user-system engagements.
7. Reprogramming of somatic cells to ES cells Somatic cell nuclear transfer Cell fusion Treatment with the extract of the pluripotent stem cells Stable expression of defined factors ( Cowan et al.,2005) ( Wilmut et al.,1997) (Takahashi and Yamanaka, 2006.)
11. Stable expression of defined factors Twenty four candidate genes play pivotal roles in the maintenance of ES cell identity base on their hypothesis. (Takahashi and Yamanaka,2006.) Myb, Kit, Gdf3, Esrrb 21 22 23 24 Ecat1 Dppa5(Esg1) Fbox15 Nanog Eras Dnmt31 Ecat8 Gdf3 Sox15 Dppa4 Dppa2 Fthl17 Sall4 Oct3/4 Sox2 Rex1 Utf1 Tcl1 Dppa3 Klf4 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 Symbol No.
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19. (1)Isolate and culture donor cells. (2)Transfect stem cell-associated genes into the cells by viral vectors. (3)Harvest and culture the cells according to ES cell culture, (4)A small subset of the transfected cells become iPS cells and generate ES-like colonies.
33. Mouse kidneys created using iPS cells A team of scientists has successfully used induced pluripotent stem (iPS) cells to create kidneys inside a mouse whose parents were genetically engineered so their offspring would not be born with the organ. (Hiromitsu Nakuchi; Mar. 10, 2009))
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