Scale up means increasing the quantity or volume of cell culture. For animal cells, the scale up strategies are dependent upon cell types or i.e. whether the cells requires matrix for attachment and growth ( adherent cell culture) or grows freely in suspended form in aqueous media. The scaling up principle for adherent cells are just to increase surface area for attachment while for suspension culture is to increase culture volume. This presentation enlightens the reader about different methods of scaling up of cells culture. Readers are also provided with sample questions for better understanding
INTRODUCTION
HISTORY
NEED OF SYNCHRONIZATION
SYNCHRONOUS CULTURES CAN BE OBTAINED IN SEVERAL WAYS:
Physical fractionation .
Chemical appro ach
CENTRIFUGAL ELUTRIATION
Inhibition of DNA synthesis
Nutritional deprivation
SYNCHRONIZATION AT LOW TEMPERATURE
CELLULAR TOTIPOTENCY
SOME HIGHLIGHTS OF CELL SYNCHRONIZATION
REFERENCES
Cellular coning refers to generation of genetically identical cells from parent cells. This presentation teaches differences between cell coning and molecular cloning and various methods of cell cloning. Sample questions are also provided for your review of concept learned
Introduction
History
Cell culture techniques
Species cloned
Approaches of cell cloning
Monolayer culture- Dilution cloning
Microtitration plate
Suspension culture- Cloning in agar
Cloning in methocel
Isolation of clone
By clonal rings
By suspension clone
Application of cell cloning
Conclusion
Reference
Scale up means increasing the quantity or volume of cell culture. For animal cells, the scale up strategies are dependent upon cell types or i.e. whether the cells requires matrix for attachment and growth ( adherent cell culture) or grows freely in suspended form in aqueous media. The scaling up principle for adherent cells are just to increase surface area for attachment while for suspension culture is to increase culture volume. This presentation enlightens the reader about different methods of scaling up of cells culture. Readers are also provided with sample questions for better understanding
INTRODUCTION
HISTORY
NEED OF SYNCHRONIZATION
SYNCHRONOUS CULTURES CAN BE OBTAINED IN SEVERAL WAYS:
Physical fractionation .
Chemical appro ach
CENTRIFUGAL ELUTRIATION
Inhibition of DNA synthesis
Nutritional deprivation
SYNCHRONIZATION AT LOW TEMPERATURE
CELLULAR TOTIPOTENCY
SOME HIGHLIGHTS OF CELL SYNCHRONIZATION
REFERENCES
Cellular coning refers to generation of genetically identical cells from parent cells. This presentation teaches differences between cell coning and molecular cloning and various methods of cell cloning. Sample questions are also provided for your review of concept learned
Introduction
History
Cell culture techniques
Species cloned
Approaches of cell cloning
Monolayer culture- Dilution cloning
Microtitration plate
Suspension culture- Cloning in agar
Cloning in methocel
Isolation of clone
By clonal rings
By suspension clone
Application of cell cloning
Conclusion
Reference
INTRODUCTION
ROLE IN CELL LINE CHARACTERIZATION
CAUSES OF TRANSFORMATION
METHODS OF TRANSFECTION
CHARACTERISTICS OF TRAANSFORMED CELLS
GENETIC INSTABILITY
IMMORTALIZATION
ABRERANT GROWTH CONTROL
TUMORIGENECITY
CHROMOSOMAL ABERATION
APPLICATION
CONCLUSION
REFERENCE
A knockout mouse is a mouse in which a specific gene has been inactivated or“knocked out” by replacing it or disrupting it with an artificial piece of DNA.
The loss of gene activity often causes changes in a mouse's phenotype and thus provides valuable information on the function of the gene.
Introduction
Primary Culture
Steps In Primary Culture
Isolation Of Tissue
Dissection And/Or Disaggregation
Types Of Primary Culture
Primary Explant Culture
Enzymatic Disaggregation
Mechanical Disaggregation
Cell Line( Finite & Continuous)
Naming A Cell Line
Choosing A Cell Line
Maintenance Of Cell Line
Conclusion
reference
A stem cell is a "blank" cell that can give rise to multiple tissue types such as a skin, muscle, or nerve cell.
Under certain physiologic or experimental conditions, they can be induced to become tissue- or organ-specific cells with special functions.
Introduction.
Properties of Stem Cells.
Key Research events.
Embryonic Stem Cell.
Stem cell Cultivation.
Stem cells are central to three processes in an organism.
Research & Clinical Application of stem cell.
Research patents.
Conclusion.
Reference.
INTRODUCTION
HISTORY
NEED OF SYNCHRONIZATION
TYPES OF SYNCHRONIZATION
(I)PHYSICAL CELL SEPARATION
(II)BLOCKADE
PHYSICAL Vs BLOCKADE SYNCHRONIZATION
CONCLUSION
REFFERENCE
Cell synchronization helps in obtaining distinct sub population of cells representing different stages of cell cycle.It helps in collecting population wide data of cells progressing through various stages of cell cycle. Immortalization, refers to cells having capability of undergoing cell division infinitely. Immortal cells are particularly preferred in cell culture to enable long time storage and use. This presentation teaches about cell synchronization, methods of cell synchronization, cellular transformation, immortalization and mechanism of immortalization.
Introduction
Terminologies
Types of tissue culture
Applications
Culturing
Sub-culturing
Cryopreservation
Detection of contaminants
In vitro transformation of cells
Cell viability
Rules for working in the Lab
Advantages
Limitations
PRODUCTION AND MAINTENANCE OF EMBRYONIC STEM CELLSANKUR SHARMA
Embryonic stem cells are pluripotent stem cells and have capacity to differentiate into all type of cells arising from 3 different germ layers i.e., ecto-, meso- and endoderm. In this presentation brief information is given about different methods for production of embryonic stem cells and their maintenance
Introduction
What is cloning?
Why we want to do cloning?
History
Technique of cell cloning
Dolly – the sheep
Species cloned
Why persue animal cloning research?
Conclusion
Introduction
What is cloning?
Why we want to do cloning?
History
Technique of cell cloning
Dolly – the sheep
Species cloned
Why persue animal cloning research?
Conclusion
INTRODUCTION
ROLE IN CELL LINE CHARACTERIZATION
CAUSES OF TRANSFORMATION
METHODS OF TRANSFECTION
CHARACTERISTICS OF TRAANSFORMED CELLS
GENETIC INSTABILITY
IMMORTALIZATION
ABRERANT GROWTH CONTROL
TUMORIGENECITY
CHROMOSOMAL ABERATION
APPLICATION
CONCLUSION
REFERENCE
A knockout mouse is a mouse in which a specific gene has been inactivated or“knocked out” by replacing it or disrupting it with an artificial piece of DNA.
The loss of gene activity often causes changes in a mouse's phenotype and thus provides valuable information on the function of the gene.
Introduction
Primary Culture
Steps In Primary Culture
Isolation Of Tissue
Dissection And/Or Disaggregation
Types Of Primary Culture
Primary Explant Culture
Enzymatic Disaggregation
Mechanical Disaggregation
Cell Line( Finite & Continuous)
Naming A Cell Line
Choosing A Cell Line
Maintenance Of Cell Line
Conclusion
reference
A stem cell is a "blank" cell that can give rise to multiple tissue types such as a skin, muscle, or nerve cell.
Under certain physiologic or experimental conditions, they can be induced to become tissue- or organ-specific cells with special functions.
Introduction.
Properties of Stem Cells.
Key Research events.
Embryonic Stem Cell.
Stem cell Cultivation.
Stem cells are central to three processes in an organism.
Research & Clinical Application of stem cell.
Research patents.
Conclusion.
Reference.
INTRODUCTION
HISTORY
NEED OF SYNCHRONIZATION
TYPES OF SYNCHRONIZATION
(I)PHYSICAL CELL SEPARATION
(II)BLOCKADE
PHYSICAL Vs BLOCKADE SYNCHRONIZATION
CONCLUSION
REFFERENCE
Cell synchronization helps in obtaining distinct sub population of cells representing different stages of cell cycle.It helps in collecting population wide data of cells progressing through various stages of cell cycle. Immortalization, refers to cells having capability of undergoing cell division infinitely. Immortal cells are particularly preferred in cell culture to enable long time storage and use. This presentation teaches about cell synchronization, methods of cell synchronization, cellular transformation, immortalization and mechanism of immortalization.
Introduction
Terminologies
Types of tissue culture
Applications
Culturing
Sub-culturing
Cryopreservation
Detection of contaminants
In vitro transformation of cells
Cell viability
Rules for working in the Lab
Advantages
Limitations
PRODUCTION AND MAINTENANCE OF EMBRYONIC STEM CELLSANKUR SHARMA
Embryonic stem cells are pluripotent stem cells and have capacity to differentiate into all type of cells arising from 3 different germ layers i.e., ecto-, meso- and endoderm. In this presentation brief information is given about different methods for production of embryonic stem cells and their maintenance
Introduction
What is cloning?
Why we want to do cloning?
History
Technique of cell cloning
Dolly – the sheep
Species cloned
Why persue animal cloning research?
Conclusion
Introduction
What is cloning?
Why we want to do cloning?
History
Technique of cell cloning
Dolly – the sheep
Species cloned
Why persue animal cloning research?
Conclusion
Stem Cell Technology and its Clinical ApplicationDr. Barkha Gupta
Dr. Barkha Gupta has been teaching Veterinary Biochemistry as well as clinical physiology at CVAS, Udaipur and PGIVER, Jaipur. She has earlier served in various capacities in the Department of Animal Husbandry, Govt. of Rajasthan. She has several publications and awards to her credit. She is the PI of M-RAJUVAS Android Educational Mobile Application for Veterinary and Animal Sciences and Kiosk Information System for Farmers/Livestock Owners. Dr. Gupta is also IFBA Certified Professional.
A Stem Cell is a Cell from the embryo ,fetus or adult that has, under certain conditions ,the ability to reproduce itself for long periods.
In the case of adult stem cells , it has the potential to reproduce through the life of an organism.
It can also give rise to specialized cells that makeup the tissues and organs of the body.
THE IMPORTANCE OF MARTIAN ATMOSPHERE SAMPLE RETURN.Sérgio Sacani
The return of a sample of near-surface atmosphere from Mars would facilitate answers to several first-order science questions surrounding the formation and evolution of the planet. One of the important aspects of terrestrial planet formation in general is the role that primary atmospheres played in influencing the chemistry and structure of the planets and their antecedents. Studies of the martian atmosphere can be used to investigate the role of a primary atmosphere in its history. Atmosphere samples would also inform our understanding of the near-surface chemistry of the planet, and ultimately the prospects for life. High-precision isotopic analyses of constituent gases are needed to address these questions, requiring that the analyses are made on returned samples rather than in situ.
(May 29th, 2024) Advancements in Intravital Microscopy- Insights for Preclini...Scintica Instrumentation
Intravital microscopy (IVM) is a powerful tool utilized to study cellular behavior over time and space in vivo. Much of our understanding of cell biology has been accomplished using various in vitro and ex vivo methods; however, these studies do not necessarily reflect the natural dynamics of biological processes. Unlike traditional cell culture or fixed tissue imaging, IVM allows for the ultra-fast high-resolution imaging of cellular processes over time and space and were studied in its natural environment. Real-time visualization of biological processes in the context of an intact organism helps maintain physiological relevance and provide insights into the progression of disease, response to treatments or developmental processes.
In this webinar we give an overview of advanced applications of the IVM system in preclinical research. IVIM technology is a provider of all-in-one intravital microscopy systems and solutions optimized for in vivo imaging of live animal models at sub-micron resolution. The system’s unique features and user-friendly software enables researchers to probe fast dynamic biological processes such as immune cell tracking, cell-cell interaction as well as vascularization and tumor metastasis with exceptional detail. This webinar will also give an overview of IVM being utilized in drug development, offering a view into the intricate interaction between drugs/nanoparticles and tissues in vivo and allows for the evaluation of therapeutic intervention in a variety of tissues and organs. This interdisciplinary collaboration continues to drive the advancements of novel therapeutic strategies.
Nutraceutical market, scope and growth: Herbal drug technologyLokesh Patil
As consumer awareness of health and wellness rises, the nutraceutical market—which includes goods like functional meals, drinks, and dietary supplements that provide health advantages beyond basic nutrition—is growing significantly. As healthcare expenses rise, the population ages, and people want natural and preventative health solutions more and more, this industry is increasing quickly. Further driving market expansion are product formulation innovations and the use of cutting-edge technology for customized nutrition. With its worldwide reach, the nutraceutical industry is expected to keep growing and provide significant chances for research and investment in a number of categories, including vitamins, minerals, probiotics, and herbal supplements.
Professional air quality monitoring systems provide immediate, on-site data for analysis, compliance, and decision-making.
Monitor common gases, weather parameters, particulates.
This pdf is about the Schizophrenia.
For more details visit on YouTube; @SELF-EXPLANATORY;
https://www.youtube.com/channel/UCAiarMZDNhe1A3Rnpr_WkzA/videos
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A brief information about the SCOP protein database used in bioinformatics.
The Structural Classification of Proteins (SCOP) database is a comprehensive and authoritative resource for the structural and evolutionary relationships of proteins. It provides a detailed and curated classification of protein structures, grouping them into families, superfamilies, and folds based on their structural and sequence similarities.
Slide 1: Title Slide
Extrachromosomal Inheritance
Slide 2: Introduction to Extrachromosomal Inheritance
Definition: Extrachromosomal inheritance refers to the transmission of genetic material that is not found within the nucleus.
Key Components: Involves genes located in mitochondria, chloroplasts, and plasmids.
Slide 3: Mitochondrial Inheritance
Mitochondria: Organelles responsible for energy production.
Mitochondrial DNA (mtDNA): Circular DNA molecule found in mitochondria.
Inheritance Pattern: Maternally inherited, meaning it is passed from mothers to all their offspring.
Diseases: Examples include Leber’s hereditary optic neuropathy (LHON) and mitochondrial myopathy.
Slide 4: Chloroplast Inheritance
Chloroplasts: Organelles responsible for photosynthesis in plants.
Chloroplast DNA (cpDNA): Circular DNA molecule found in chloroplasts.
Inheritance Pattern: Often maternally inherited in most plants, but can vary in some species.
Examples: Variegation in plants, where leaf color patterns are determined by chloroplast DNA.
Slide 5: Plasmid Inheritance
Plasmids: Small, circular DNA molecules found in bacteria and some eukaryotes.
Features: Can carry antibiotic resistance genes and can be transferred between cells through processes like conjugation.
Significance: Important in biotechnology for gene cloning and genetic engineering.
Slide 6: Mechanisms of Extrachromosomal Inheritance
Non-Mendelian Patterns: Do not follow Mendel’s laws of inheritance.
Cytoplasmic Segregation: During cell division, organelles like mitochondria and chloroplasts are randomly distributed to daughter cells.
Heteroplasmy: Presence of more than one type of organellar genome within a cell, leading to variation in expression.
Slide 7: Examples of Extrachromosomal Inheritance
Four O’clock Plant (Mirabilis jalapa): Shows variegated leaves due to different cpDNA in leaf cells.
Petite Mutants in Yeast: Result from mutations in mitochondrial DNA affecting respiration.
Slide 8: Importance of Extrachromosomal Inheritance
Evolution: Provides insight into the evolution of eukaryotic cells.
Medicine: Understanding mitochondrial inheritance helps in diagnosing and treating mitochondrial diseases.
Agriculture: Chloroplast inheritance can be used in plant breeding and genetic modification.
Slide 9: Recent Research and Advances
Gene Editing: Techniques like CRISPR-Cas9 are being used to edit mitochondrial and chloroplast DNA.
Therapies: Development of mitochondrial replacement therapy (MRT) for preventing mitochondrial diseases.
Slide 10: Conclusion
Summary: Extrachromosomal inheritance involves the transmission of genetic material outside the nucleus and plays a crucial role in genetics, medicine, and biotechnology.
Future Directions: Continued research and technological advancements hold promise for new treatments and applications.
Slide 11: Questions and Discussion
Invite Audience: Open the floor for any questions or further discussion on the topic.
2. What are stem cells?
• Stem cells are undifferentiated or partially differentiated cells that can
differentiate into various types of cells and proliferate indefinitely. It can divide
and renew themselves over a long time.
• They have the potential to become specialized cells like muscle cells,blood
vessels and brain cells.
• There are mainly two types of stem cells
• Embryonic stem cells
• Adult stem cells
4. Types of stem cell markers( based on the source of origin)
1. HAEMATOPOIETIC STEM CELLS
Bone marrow
2. MESENCHYMAL / MARROW STROMAL CELLS
Bone marrow, Culture into osteoblasts
3. NEURAL STEM CELLS
Neurons, Astrocytes, Oligodendrocytes
5. WHAT ARE STEM CELL MARKERS?
• Stem cell markers are genes and their genes and their proteins
products used by scientists to isolate and identify stem cells.
• Stem cells can also be identified by functional assays
• They are considered the gold standard for the identification and
therapeutic purposes.
• Stem cell markers are given short hand names based on the
molecules that it bind to the stem cell surface receptors. A cell
that has the receptor stem cell antigen -1,on it’s surface, is
identified as Sca-1.CD34 has been considered the most critical
marker for haematopoietic stem cells (HSCs)
7. EMBRYONIC STEM CELL MARKERS
• Embryonic stem cell (ESC) markers are molecules specifically expressed in ES cells.
• These markers are critical for characterization and elucidation for the mechanism of ESC
pluripotent maintenance and self-renewal, therefore helping to accelerate the clinical
application of embryonic stem cells.
• POU5F1 gene. Oct-4 is a homeodomain transcription factor of the POU family. It is
expressed in totipotent embryonic stem and germ cells. A critical level of Oct-4 expression
is required to sustain stem cell self-renewal and pluripotency.
• It is involved in the self-renewal of undifferentiated embryonic stem cells. It is a frequently
used marker for undifferentiated cells. It is also the first and most recognized marker used
for the identification of totipotent ES cells.
8. CELL SURFACE PLURIPOTENCY MARKERS
TRA-1-60 and TRA-1-81 antigens on the human embryonal carcinoma (EC) cells and
human pluripotent stem cell surfaces are widely used as markers in identifying and
isolating ESCs Besides, they are also routinely used to assess the pluripotency status
of induced pluripotent stem (iPS) cells. They are also expressed in teratocarcinoma and
EG cells Both TRA-1-60 and SSEA4 are both expressed on human embryonal
carcinomas and on human embryonic stem cells Upon differentiation, TRA-1-60 and
SSEA4 expression levels decrease and SSEA1 expression increases on human
embryonic stem cells over time when treated with Retinoic acid Besides, they also
express CD349/frizzled-9, stage-specific embryonic antigen (SSEA)-4, Oct-4, Nanog,
and nestin . Oct-4 and Nanog, as well as several cell surface markers (SSEA-1, SSEA-
4, TRA-1-60, and TRA1-81) have been used to characterize mouse and human
embryonic stem cells
9. Pluripotent embryonic stem cells (ESCs) correspond to
cells within a developing embryo that have the capacity
to generate all the embryonic germ layers (i.e.
endoderm, mesoderm and ectoderm), and are able to
give rise to all cell types in the body. ESCs may be
derived from developing embryos at the pre-
implantation blastocyst stage, and specifically from cells
within the inner cell mass (ICM). In mice, the
pluripotent state of ICM cells (mESCs) is often referred
to as a “naïve” state. Following blastocyst implantation,
ICM derived cells or mouse epiblast stem cells retain
self-renewal capacity but are in a “primed” state of
pluripotency.
PLURIPOTENT EMBRYONIC STEM CELLS
10. CANCER STEM CELL MARKERS
• Cancer stem cells oncogenically transformed cancer stem cells (CSCs) are defined by their
ability for self- renewal are defined by their ability for self-renewal and multipotency.
• The CSC hypothesis states that although CSCs represent a rare population of cells within a
tumour, their high tumorigenic capacity drives tumorigenesis. Due to their intrinsic stem cell-
like properties,
CSC proliferation generates more CSCs and generates more CSCs and all the differentiated cell
types that compose the bulk of the tumour.
• CD44 and CD24 have been used extensively in combinations or with other putative markers to
isolate CSCs from solid tumours.
11. CANCER STEM CELL MARKER ORGAN
CD44 BREAST
CANCER
HEAD AND NECK
PROSTATE
BRAIN
COLON
CD133 BRAIN
PROSTATE
COLON
CD24 COLON,STOMACH, GALLBLADDER AND OVARY
PANCREAS
ALDH1 HEMATOPOIETIC
BREAST
12. OSTEOPROGENITOR STEM CELL MARKER
Bone homeostasis is a dynamic process relying on the balance of deposition by osteoblasts and
resorption by osteocytes. Osteogenesis is not only responsible for the continuous remodelling
Of bone tissue but also for the continuous remodelling of bone tissues but also crucial for the
maintenance of bone tissue but also crucial for the maintenance of bone, size , shape and
Integrity. Disruptions of bone homeostasis accompany disorders that include osteoporosis,
arthritis, and many inheritable skeletal diseases. Cell type specific markers were developed
to identify mesenchymal stem cells that have differentiated to osteoprogenitors, osteoblasts,
or osteocytes. Certain osteoprogenitors can be distinguished by the expression pattern of TGF- beta
bFGf, BMP- 2 and bFGF or gremlin 1. Certain markers like ALPP, MCAM , collagen I, collagen II,
RUNX2, decorin , and TPO are also used . A Marconi et al use Sox5/6/9 as the markers for the
perichondral progenitor cells.
13. OSTEOBLAST MARKERS
1. Osteoblast are the skeletal cells which constitute the extracellular matrix
of bone , typically arising in the embryo.
2. The expression of osteoblastic markers ( CBFA-1/Runx2, ALP, SP7/
Osteria, M-CSF, AND RANK-L) supports the immunohistochemical
findings.
1. Immunofluorescence microscopy also revealed the existence of TRAP
and MHC class II positive cells, suggesting the presence of osteoclasts
and dendritic cells, respectively.
14. OSTEOCYTE MARKERS
1. Osteocytes are the most abundant cells in bone, and their death by microdamage
has been suggested to be the major event leading in the initiation of osteoclastic
bone resorption .
2. As osteocytes secrete several factors: TGF beta, RANKL, and MCSF which may
affect the recruitment and function of osteoclasts after being released in the
marrow compartment osteocytes produces clerostin which negatively interacts
Wnt signalling and therefore inhibits bone formation. Osteocytes also produce
DKK which targets the Wnt signaling pathway to control bone formation
negatively.
15. MYOGENIC PRECURSOR MARKERS
1. Myogenesis is the generation of muscle tissue when committed
muscle stem cells or myoblasts proliferate into multinucleate into
myotubes in the embryo. In Adult tissue , muscle- derived stem cells
are distinct population of cells from muscle satellite cells which
promote muscle stem cell regeneration to injury and disease. A
cocktail of the myogenic marker CD56, the endothelial cell marker
UAE-1 receptor and the perivascular cell marker CD146can be used to
mark the myogenic precursor markers.
2. ABCG2 and M- Cadherin-15/ M- Cadherin, Caveolin -1, CD34 are some
myogenic precursor markers
16. MESENCHYMAL STEM CELL MARKERS
Mesenchymal stem cell markers are multipotent mesoderm-derived progenitor
cells with the capacity to differentiate into adipose, bone, cartilage and muscle
tissues providing wide-ranging therapeutic avenues. Adult mesenchymal stem
cells can be isolated from a stroma of the bone marrow. MSCs express a panel
of key markers including markers CD10, CD13, CD73,CD105 and CD271. MSC
derived from bone marrow ( BMMSCs) express a postnatal stem cells capable
of self-renewing and differentiating into osteoblasts, chondrocytes, adipocytes
and neural cells.
17. NEURAL STEM CELL MARKERS
1. Neural stem cells are unique cells endowed with self-renewing, multipotent, responsible
for the generation the main phenotypes of the nervous system.
2. NSCs have the unique potential to produce clonally related progeny that upon
differentiation ,constitute the central nervous system developed by neurons progeny that
upon differentiation developed by neurons, astrocytes, oligodendrocytes , and the
ependymal cells. Many neural cell types were identified and isolated by using cell surface
marker expression.
3. CD133 is expressed on the surface of Neural stem cell and has ben widely used to isolate
Neural stem cell from human brain.
4. CD15, a stage -specific embryonic antigenic-1 is now identified as a marker of Neural stem
cell and radial glia from the subventricular zone(SVZ) in mice.
18. SKIN STEM CELL MARKERS
1. Multipotent skin stem cells or epidermal stem cells are responsible for tissue homeostasis
during normal cell turnover and wound healing. Such skin stem cells were found to reside
within a niche within the hair follicle. Although , predominantly under quiescence or a slow
cell cycle, epidermal stem cells can be stimulated to proliferate and differentiate into the
specialized cells that composed a hair follicle during wound healing. K15 protein expression
not only marks on epidermal stem or progenitor cell subpopulation and also represents
basal-like cells during the epidermal differentiation program.
2. Some integrins have been suggested as markers for epidermal stem cells. The ᾳ₆ᵦ₄ integrin
associated with and ᾳ₃ᵦ₁ integrins associated with the lateral ( mainly) and basal (minor)
surfaces of epidermal basal cells.
19. INTESTINAL STEM CELL MARKERS
It is now widely recognized that multipotent intestinal stem cells that reside
between villi within the crypts drive continuous replenishment of the epithelial
cells. Intestinal stem cells or crypt cells have the ability to self-renew and
regenerate the intestinal tissue by differentiating into various intestinal cells
including endocrine cells, enterocytes, goblet cells, and Paneth cells. Wang et al
demonstrate that Lrig1 is implicated in maintaining the intestinal epithelial
homeostasis and marks stem cells in the intestine . Lgr5 is used as a stem cell
marker of the intestinal epithelium and the hair follicle however, intestinal
organoids can also develop from Lgr5- cells.
20. ADIPOSE- DERIVED STEM CELL MARKERS
1. Adipose tissue is composed primarily of adipocytes, cells that function to store energy in the
form of lipids droplets. Additionally, pluripotent progenitor cells called adipose tissue by the
expression of markers such as Integrin family members, CD44 and ICAM-1/CD54. Like
mesenchymal stem cells, Multipotent adipose derived stem cells have been shown to
differentiate into cells of the mesodermal lineage including adipocytes, chondrocytes,
osteoblasts, osteoclasts and myoblasts in vitro.
2. Adipose- derived stem cell markers express characteristics adhesion and receptor molecules
,surface enzymes, extracellular matrix and cytoskeleton proteins associated with the
stromal cell phenotype of ASCs resembles that of bone marrow derived mesenchymal stem
cells or stomal cells (MSCs) and skeletal muscle- derived cells. Some of the adipose derived
stem cell markers are:-
CD13, CD29 , CD 44, CD71 , CD71 , CD90 , CD105/SH2 and SH3 surface markers
21. OVARY AND TUBAL STEM CELL MARKERS
• Lgr5 is a stem cell marker for multiple epithelia , and has recently been shown to the marker of stem/
progenitor cells in the ovary and tubal epithelia.
• Various Ovarian cancer stem cell markers such as ALDH1,CD44, AND CD117, ALDH1 is an enzyme involved in
the metabolism of retinoic acids and probably plays a central role in differentiation ALDH1 is also involved in
cell surface glycoprotein involved in invasion and metastasis via the activation of thePI3K/AKT pathway.
• CD117 is a protooncogene (c-kit) that encodes for a tyrosine kinase receptor and plays an important role in
oncogenic process such as cell proliferation and tumor development.
• An ovarian serous carcinogenic sequence was recently described and it has been suggested that most high-
grade serous ovarian cancers( HGSC) would have a tubal origin and a tubal precursor lesion called “serous
tubal intraepithelial carcinomas (STICs)
22. ECTODERM AND ENDODERM MARKERS
Ectoderm is one of the three primary germ cell layers in the very early embryo. The other two
layers are the mesoderm (middle layer) and endoderm (most proximal layer), with the
ectoderm as the most exterior (or distal) layer. Certain factors mark the ectoderm including
Otx2, Chordin, p63/TP73L, FGF-8, Pax2. FoxJ3, Pax6, GBX2, SOX1, Nestin, beta- Tubulin, and
Noggin. Endoderm formation depends on two sequential positive feedback loops mediated
by Cripto and Bmp4/Wnt3 that are activated by mature or uncleaved Nodal, respectively, to
sustain Nodal signaling from implantation throughout gastrulation ENDM1 and Flk1 have
been used as a definitive mouse endodermal cell marker and a mesoderm cell marker,
respectively
23. CYTOKERATIN MARKERS
1. Cytokeratin markers are the intermediate filaments can serve as a marker system epithelial
cells at different stages of cellular differentiation. The expression of these cytokeratins relies
on the type of epithelium and their expression also changes during the course of normal
epithelial cell differentiation. Cytokeratin markers can provide valuable supporting evidence
in combination with a standard marker to subfractionate populations of stem, progenitor
and differentiated cells.
2. One of the most well-characterized cytokeratin expression patterns is available in skin, where
CK15 expression is restricted to the bulge region multipotent stem cells, but replaced by CK5
As differentiation proceeds, CK5 is replaced by CK10 and eventually the terminal
differentiation marker involucrin . Now it is understood that CK14 and CK5 are expressed in
urothelial basal cells, CK8 and CK18 mark intermediate cells and CK20 are expressed only in
terminally differentiated umbrella cells
24. REFERENCES
1. Pazhanisamy, Senthil. “Stem Cell Markers.” Materials and Methods, vol.
MATER METHODS 2013;3:200, 7 Mar. 2020,
www.labome.com/method/Stem-Cell-Markers.html.
2. Zhao, Wenxiu, et al. “Embryonic Stem Cell Markers.” Molecules, vol. 17, no.
6, 25 May 2012, pp. 6196–6236, 10.3390/molecules17066196. Accessed 1
Mar. 2020.