This document discusses stem cell therapy and its potential to treat various "no hope disorders". It provides an overview of stem cell sources and types, including adult stem cells from bone marrow, blood, dental pulp and other tissues. Mesenchymal stem cells are highlighted as having advantages for therapy due to their plasticity and low risk of rejection. A range of conditions are described as effectively treated with stem cell therapy, including diabetes, neurological disorders, bone/cartilage disorders and liver/kidney diseases. The document promotes an Indian stem cell company that offers various banking and treatment services.
What is Stem Cell ?
History of Stem Cells ?
Stages of Embryogenesis
Blastocyst Diagram
Three types of stem cells
Differentiation of ESC
Adult Stem Cells
Bone Marrow
Umbilical cord stem cells
Factors known to affect stem cells
Niche cells activates Stem cells
Regenerative Medicine : Indian Scenario
Stem Cell Technology and its Clinical ApplicationDr. Barkha Gupta
Dr. Barkha Gupta has been teaching Veterinary Biochemistry as well as clinical physiology at CVAS, Udaipur and PGIVER, Jaipur. She has earlier served in various capacities in the Department of Animal Husbandry, Govt. of Rajasthan. She has several publications and awards to her credit. She is the PI of M-RAJUVAS Android Educational Mobile Application for Veterinary and Animal Sciences and Kiosk Information System for Farmers/Livestock Owners. Dr. Gupta is also IFBA Certified Professional.
What is Stem Cell ?
History of Stem Cells ?
Stages of Embryogenesis
Blastocyst Diagram
Three types of stem cells
Differentiation of ESC
Adult Stem Cells
Bone Marrow
Umbilical cord stem cells
Factors known to affect stem cells
Niche cells activates Stem cells
Regenerative Medicine : Indian Scenario
Stem Cell Technology and its Clinical ApplicationDr. Barkha Gupta
Dr. Barkha Gupta has been teaching Veterinary Biochemistry as well as clinical physiology at CVAS, Udaipur and PGIVER, Jaipur. She has earlier served in various capacities in the Department of Animal Husbandry, Govt. of Rajasthan. She has several publications and awards to her credit. She is the PI of M-RAJUVAS Android Educational Mobile Application for Veterinary and Animal Sciences and Kiosk Information System for Farmers/Livestock Owners. Dr. Gupta is also IFBA Certified Professional.
iPSCs are pluripotent; unlike ESC, iPSCs are not derived from the embryo, but instead created from differentiated cells in the lab through a process – cellular reprogramming.
For better view, press F5.
As we go through our lives each of us will have very different needs for our own healthcare.
Scientist's are constantly researching to make medical care treatment more personalized.
One way they are doing this is by-
Stem Cells therapy
Stem-cell therapy is the use of stem cells to treat or prevent a disease or condition.
It is also known as regenerative medicine, promotes the reparative response of diseased, dysfunctional or injured tissue using stem cells or their derivatives.
It is the next chapter of organ transplantation and uses cells instead of donor organs, which are limited in supply.
What are Stem cells?
Stem cells are called “master cells”
Stem cells are cells that are undifferentiated.
What are Stem cells?
Steam cells have the potential to become all other kinds of cells in our body.
What are Stem cells?
Types of Stem cells
How stem cell therapy works?
Disease cured by stem cell therapy.
Spinal Cord Injuries
Stem cell treatment of Diabetes mellitus type 1 & 2
Stem cell treatment of Stroke
Cancer treatment
Heart damage
Baldness
Tooth implanting
Deafness and blindness
Have stem cells already been used to treat diseases?
Ethical Consideration of Stem Cell Therapy
As the research method mainly focused on Embryonic Stem Cells, which involves taking tissue from an aborted embryo to get proper material to study. This is typically done just days after conception or between the 5th and 9th week.
Since then, researchers have moved on to more ethical study methods, such as Induced Pluripotent Stem Cells (iPS). iPS is artificially derived from a non-pluripotent cell, such as adult somatic cells.
Nowadays stem cell treatment has been spreaded throughout the world. It has also been grown commercially in developed countries.
It is thought that one day it may be the major key to treat various diseases.
Using stem cells to conduct medical research and treat disease is acceptable?
Don’t know
No
Yes
Do you approve of the extraction of stem cells from human embryos for medical research?
Don’t know
No
Yes
Imagine that you have been told you have an illness that cannot be cured or what if your body has been irreversibly paralysed. There is no hope. But there is a science that could change that. It’s Called Stem Cell Research and it’s an important step in the medical revolution. But it comes with controversies as it uses Human Embryos’ as Raw Material.
But something astounding happened in the year 2006 that removed the usage of surplus embryos from the equation altogether. It’s about a brand new technology that can turn back the clock on your body cells. This is cutting edge of science where new developments are happing all the time. The iPSCs could be the potential medicine of 21st century. So what are stem cells? Why do they Matter? What are iPSCs and how it changed the biological rules?
This slide is about the potential uses of stem cells. It describes how they are useful and also puts froward the extraction process and the ares in which stem cells prove to be extremely useful. This slide also lists the various from of cells and the difference between stem cells and the normal differentiated cells. It is also richly supplied with photos and content which would altogether increase the quality of the slide. Hope you enjoy and learn. Please do like and follow. Share with your friends who might benefit from this.
history ,definition,type of stem cells , characters of stem cells , source, stem cell banking , indications of stem cell therapy ,applications in gynaecology
A stem cell is a "blank" cell that can give rise to multiple tissue types such as a skin, muscle, or nerve cell.
Under certain physiologic or experimental conditions, they can be induced to become tissue- or organ-specific cells with special functions.
What are stem cells? This presentation provides an overview of multiple different stem cells including embryonic stem cells, mesenchymal stem cells, cancer stem cells, induced pluripotent stem cells, hematopoietic stem cells and neural stem cells.
If the cell is able to form all cell types of the embryo & adult (Fertilized egg cell) Totipotent stem cell
Stem cell able to differentiate into all 3 germ layers Pluripotent stem cell (Embryonic stem cell)
Multipotent stem cell Differentiate to form cells of some but not all 3 germ layers (Bone, cartilage, connective tissue)
Unipotent stem cell Able to form just one other cell type (Spermatogonia)
Embryos created in vitro fertilization
Aborted embryos
Limited tissues (bone marrow, muscle, brain)
Discrete populations of adult stem cells generate replacements for cells that are lost through normal wear and tear, injury or disease
Placental cord
Baby teeth
Diabetes patients lose the function of their insulin-producing beta cells of the pancreas
Human embryonic stem cells may be grown in cell cultures and stimulate to form insulin-producing cells , that can be transplanted into the patients
Pancreas is digested with collagenase that frees islets from surrounding cells
Centrifugation of isolates containing mainly alpha and beta cells, purified islets beta cells
Transplanted through a catheter into the liver where they become permanently established Caused when key brain cells that produce message carrying chemical/neurotransmitter (dopamine) die off.
Symptoms start with the patients trembling and can end up paralyzed
Harvesting of stem cells from patients bone marrow, foetus or any other source
Culturing of harvested stem cells in lab conditions - to get high concentrations of stem cells
Then purified and high concentration of stem cells are surgically injected in the brain of patient.
iPSCs are pluripotent; unlike ESC, iPSCs are not derived from the embryo, but instead created from differentiated cells in the lab through a process – cellular reprogramming.
For better view, press F5.
As we go through our lives each of us will have very different needs for our own healthcare.
Scientist's are constantly researching to make medical care treatment more personalized.
One way they are doing this is by-
Stem Cells therapy
Stem-cell therapy is the use of stem cells to treat or prevent a disease or condition.
It is also known as regenerative medicine, promotes the reparative response of diseased, dysfunctional or injured tissue using stem cells or their derivatives.
It is the next chapter of organ transplantation and uses cells instead of donor organs, which are limited in supply.
What are Stem cells?
Stem cells are called “master cells”
Stem cells are cells that are undifferentiated.
What are Stem cells?
Steam cells have the potential to become all other kinds of cells in our body.
What are Stem cells?
Types of Stem cells
How stem cell therapy works?
Disease cured by stem cell therapy.
Spinal Cord Injuries
Stem cell treatment of Diabetes mellitus type 1 & 2
Stem cell treatment of Stroke
Cancer treatment
Heart damage
Baldness
Tooth implanting
Deafness and blindness
Have stem cells already been used to treat diseases?
Ethical Consideration of Stem Cell Therapy
As the research method mainly focused on Embryonic Stem Cells, which involves taking tissue from an aborted embryo to get proper material to study. This is typically done just days after conception or between the 5th and 9th week.
Since then, researchers have moved on to more ethical study methods, such as Induced Pluripotent Stem Cells (iPS). iPS is artificially derived from a non-pluripotent cell, such as adult somatic cells.
Nowadays stem cell treatment has been spreaded throughout the world. It has also been grown commercially in developed countries.
It is thought that one day it may be the major key to treat various diseases.
Using stem cells to conduct medical research and treat disease is acceptable?
Don’t know
No
Yes
Do you approve of the extraction of stem cells from human embryos for medical research?
Don’t know
No
Yes
Imagine that you have been told you have an illness that cannot be cured or what if your body has been irreversibly paralysed. There is no hope. But there is a science that could change that. It’s Called Stem Cell Research and it’s an important step in the medical revolution. But it comes with controversies as it uses Human Embryos’ as Raw Material.
But something astounding happened in the year 2006 that removed the usage of surplus embryos from the equation altogether. It’s about a brand new technology that can turn back the clock on your body cells. This is cutting edge of science where new developments are happing all the time. The iPSCs could be the potential medicine of 21st century. So what are stem cells? Why do they Matter? What are iPSCs and how it changed the biological rules?
This slide is about the potential uses of stem cells. It describes how they are useful and also puts froward the extraction process and the ares in which stem cells prove to be extremely useful. This slide also lists the various from of cells and the difference between stem cells and the normal differentiated cells. It is also richly supplied with photos and content which would altogether increase the quality of the slide. Hope you enjoy and learn. Please do like and follow. Share with your friends who might benefit from this.
history ,definition,type of stem cells , characters of stem cells , source, stem cell banking , indications of stem cell therapy ,applications in gynaecology
A stem cell is a "blank" cell that can give rise to multiple tissue types such as a skin, muscle, or nerve cell.
Under certain physiologic or experimental conditions, they can be induced to become tissue- or organ-specific cells with special functions.
What are stem cells? This presentation provides an overview of multiple different stem cells including embryonic stem cells, mesenchymal stem cells, cancer stem cells, induced pluripotent stem cells, hematopoietic stem cells and neural stem cells.
If the cell is able to form all cell types of the embryo & adult (Fertilized egg cell) Totipotent stem cell
Stem cell able to differentiate into all 3 germ layers Pluripotent stem cell (Embryonic stem cell)
Multipotent stem cell Differentiate to form cells of some but not all 3 germ layers (Bone, cartilage, connective tissue)
Unipotent stem cell Able to form just one other cell type (Spermatogonia)
Embryos created in vitro fertilization
Aborted embryos
Limited tissues (bone marrow, muscle, brain)
Discrete populations of adult stem cells generate replacements for cells that are lost through normal wear and tear, injury or disease
Placental cord
Baby teeth
Diabetes patients lose the function of their insulin-producing beta cells of the pancreas
Human embryonic stem cells may be grown in cell cultures and stimulate to form insulin-producing cells , that can be transplanted into the patients
Pancreas is digested with collagenase that frees islets from surrounding cells
Centrifugation of isolates containing mainly alpha and beta cells, purified islets beta cells
Transplanted through a catheter into the liver where they become permanently established Caused when key brain cells that produce message carrying chemical/neurotransmitter (dopamine) die off.
Symptoms start with the patients trembling and can end up paralyzed
Harvesting of stem cells from patients bone marrow, foetus or any other source
Culturing of harvested stem cells in lab conditions - to get high concentrations of stem cells
Then purified and high concentration of stem cells are surgically injected in the brain of patient.
Dr. Kenneth Dickie from Royal Centre of Plastic Surgery in Barrie, Ontario explained the use of stem cells technology in plastic surgery.
If you have any questions, please contact Dr. Kenneth Dickie at http://royalcentreofplasticsurgery.com/
This presentation deals with stem cell therapy & new avenues in stem cell therapy. It also discusses latest advances such as treatment against baldness, multiple sclerosis, type 1 diabetes, spinal cord injury, demyelinating diseases, deafness, eye, Parkinson's disease. Also discusses about umbilical cord stem cells and finally clinical trials without patients (organs on chips).
Blood production agency. all types of blood cellls are produced in it. to understand it is the need of this era. it also will help in the physiology of blood making mechanism.
Get information of what are stem cells, sources of stem cells, what is umbilical cord and the umbilical cord blood, what us HLS matching etc and many more.
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4. The fascinating new world of
STEM CELL THERAPY :
a medical revolution at the corner
Welcome
to
5. Future of Stem Cell Medicine
• The Indian market potential slated to be in the range of US$ 500 million.
• More than 45,000 people across the world receiving stem cell
transplant every year.
• Potential to reduce pharmaceutical R&D by 25% each year, with
savings of up to US $25 million in each drug market.
• Potential to be the next revolution in the field of medicine.
LifeCare – ReeCure Centre
of STEM CELL Therapy
6. “The Need”
• The physical misery
• Psychological trauma
Of “No Hope Disorder” is great!!
• No cure available for most of these diseases
7. Rationale of is stem cell
Therapy ??
Introduction of adult stem cells into
damaged tissue in order to treat
injuries / Disease in
“No Hope Disorders”
9. • Is it really working?
• Why not studies are coming from premier institutes of
country ?
• Is it really possible to segregate Mesenchymal cells?
• Differentiation of cells out side body possible in India???
• If it is too good then, why not NOBEL PRIZE given ?
• ?
• ??
• ???
• Is this therapy available in India?
Questions ????
11. We are sure there are a lot of doubts plaguing
your mind about STEM CELL BANKING and
STEM CELL THERAPY.
Let us help you to ERASE these doubts.
Doubts… on SCT
12. 1913 the great Spanish neuroscientist Santiago Ramón y Cajal
pronounced “that in adult Neurone & the nerve paths –
Everything may die, nothing may be regenerated”.
Nerve Cell
Cannot
Regenerate ?
15. Major Milestones
Which Triggered Adult Stem Cell
Research ?
• Since1970’s,
BONE MARROW TRANSPLANTS
have been used for treatment of
Immunodeficient &
leukemia (with Good results).
16. 2nd Breakthrough…
History of Human Embryonic
Stem Cell Research
In 1998, James Thomson (University of
Wisconsin-Madison) isolated cells from the inner
cell mass of the blastocyst, and developed the
first human embryonic stem cell line in culture.
17. Prediction was made of Making cells
and replacing all the diseased cells?
& even gametes
19. Stem Cell History
• 1970 – BM transplant for leukaemia
• 1998 - Researchers first extract stem cells from human
embryos
• 1999 - First Successful human transplant of insulin-making cells
from cadavers
• 2001 - President Bush restricts federal funding for embryonic
stem-cell research
• 2002 - Juvenile Diabetes Research Foundation International
creates $20 million fund-raising effort to support stem-
cell research
• 2003?? - California ok stem cell research
• 2004 - Harvard researchers grow stem cells from embryos
using private funding
• 2004 - Ballot measure for $3 Billion bond for stem cells
• 2006-7 – Private Banking for cord & cord blood in india.
• 2014 – Public cord banking Kolkatta – Inst. Tropical med.
20.
21. What are Stem Cells
• Ability to differentiate
• Ability to regenerate
22. Stem Cell is a
•young,
•primitive and unspecialized cell with a
remarkable potential to renew,
•Differentiate and
Develop into any desired tissue or organ of
the body.
What is stem cells ?
23. Self renewal: Unlimited proliferative potential.
Differentiation: Differentiate into various cell types
Pluripotency,
Multipotency,
Unipotency)
Plasticity : Trans-differentiation
Regeneration potential: a means of Repair
Characteristic Features
25. Cells having infinite self renewing capacity and
potential for differentiation.
What are Stem Cells?
26. 1000 IEC ICAs in
biocompatible capsule
IP Tx
STZ Diabetic mice
Blood glucose analysis of STZ induced
diabetic mice transplanted with hUCMSCs and
Islet like clusters (ILC)
0 10 20 30
0
100
200
300
400
500
hUCMSCs
ILCs
Normal
Diabetic control
Days after transplantation
Bloodglucoseconcentration
mg/dl
0 25 50 75 100 125 150
0
100
200
300
400
ILC
diabetic control
non diabetic control
time (mins)
Bloodglucoseconcentration
mg/dl
Glucose
Tolerance
Test
Fasting blood glucose concentration of diabetic mice after transplantation of ILCs showed
that the hyperglycemic mice reverted back to normoglycemia. Intraperitoneal glucose
tolerance test was performed on 45th day, post-transplantation. Mice transplanted with ILCs
showed similar blood glucose profile as compared to the normal non diabetic controls.
27. How MSCs work In vivo
Under normal circumstances MSC contribute to tissue maintenance, contributing to
immune homeostasis by avoiding unnecessary immune cell response.
With damaged tissue,
harmonious interaction between
MSC and endothelial cells are
disrupted, allowing blood
extravasations , blood clot form,
platelet release multitude of
bioactive molecule, immune
cells are attracted where they
start immune surveillance and
immune response.
MSC secrete bioactive molecules that
exert anti-apoptotic, immunomodulatory ,
angiogenic , anti-scaring , anti-fibrotic
and chemoattractant effects
28. Source of Adult Stem cells
• Autologus,
• Allogeneic or
• Xenogenic
Fetal tissue is the best current tissue source for
human stem cells,
However ethical issues are a major concern.
(ICMR – Autologous)
29. The embryos of
aborted fetuses
Umbilical cord
and cord blood of a
new born baby
Bone Marrow
Peripheral
Bloodstream
Adult Tissues Amniotic Fluid
MENSTRUAL BLOOD Dental Pulp
Sources of Stem Cells
Hair
31. Cord Blood Stem Cells
• Can be used only
to cure chronic
blood-related
disorders such as
sickle cell
disease,
Thalasemia, and
leukaemia.
32. Umbilical Cord Blood Stem Cell
Transplant
• Umbilical cord blood
stem cell transplants are
less prone to
rejection than either
bone marrow or
peripheral blood stem
cells. This is probably
because the cells have
not yet developed the
features that can be
recognized and attacked
by the recipient's
immune system
33. Placental Blood as a Source of Hematopoietic
Stem Cells for Transplantation into
UNRELATED RECIPIENTS
Joanne Kurtzberg, M.D.et al
34. PLASTICITY: The ability of one tissue to produce cells of other developmentally
unrelated tissues
Traditionally, stem cells were believed to be Lineage Restricted and
Organ Specific.
Bone marrow stem cells:
The gold standard
35. AUTOLOGUS – STEM CELLS
• While most blood stem
cells reside in the bone
marrow, a small number
are present in the
bloodstream. These
multipotent peripheral
blood stem cells, or
PBSCs, can be used
just like bone marrow
stem cells to treat
leukaemia, other
cancers and various
blood disorders
38. Allogeneic – Stem Cells
• Sources of stem cells
from another donor
(allogeneic) are
primarily relatives
(familial- allogeneic) or
completely unrelated
donors (unrelated-
allogeneic). The stem
cells in this situation
are extracted from
either the donor's body
or cord blood
39. Xenogeny - Stem Cells
• In this stem cells from
different species are
transplanted, e.g. porcine
fetal ventral mesencephalic
(FVM) xenotransplants for
Parkinson's disease.
This has no major ethical
concerns and a large amount
of tissue is available,
however life long
immunosupression and risk
of rejection are the major
limitations
41. HOMING
• The cells reach
the target cell or
tissue
PLASTICITY
• Cells transform
into target cell or
tissue
ENGRAFTMENT
• Symbiotically
integrates with
cell or tissue for
the persons
lifetime
THE SUCCESS OF STEM CELL
THERAPY DEPENDS ON LAB.
42. These are
• Mono Nuclear cells ( MNC )
• Hematopoietic Stem Cells [HSC] family:
CD34+; CD45; CD133
• Mesenchymel count in M.N.C is just 0.001%
• Mesenchymel stem cell [MSC]: CD73; CD90;
CD106 Etc ..........
Classification Of Stem Cells
43. Differentiation with different cluster
coding/desire cells type ( as per disorder)
through culture.. (Mesenchymal types are -
CD 73, CD 90, CD 105 etc . .. . .
• Multiplication up label of required cells
quantity.( near to 1.5 Cr.).
• Composition of all cells like Mnc, Hsc & Msc.
Technology Behind Success
45. Homogeneous
population and
high rate of cell
division
No risk of
rejection – USED
across HLA
barrier
No ethical
issues
Immune
privileged
Greater
potency of
cultured
expanded
product
Precise
identification
Ease of
isolation and
scale up
Genetic
stability
Advantages of
Mesenchymal
Stem Cells
No risk of
teratoma
formation
Pluripotent
Efficient large
scale expansion
Lower cost of
cell culture
process
Why MSCs ?
56. Not Effective/ Need More Trails
Acute Leukaemia • Inherited Platelet Abnormalities
• Chronic Leukaemia • Other Inherited Disorder
• Myelodysplastic Syndromes • Inherited Metabolic Disorders
• Marrow Failure • Histiocytic Disorders
• Thalassemia
• Inherited Erythrocyte
Abnormalities
• Myeloproliferative Disorders
• Inherited Immune System
Disorders
• Lymphoproliferative
Disorders
• Plasma Cell Disorders
• Phagocyte Disorders • Other Malignancies
57. Drug Testing - Quicker
• Stem cells could allow scientists to test
new drugs using human cell line which
could speed up new drug development.
• Only drugs that were safe and had
beneficial effects in cell line testing would
graduate to whole animal or human
testing.
• It would allow quicker and safer
development of new drugs.
58. Let’s
Create a better tomorrow
for
Spread the message
Please create Public Awareness
Spinal injury
My cord blood treated
Bhai’s thalassemia major Cured of insulin dep. DM
Super man
Ajit Jogi
59. Only company in India with 9 type of stem cell
banking and treatment through Reecure
50 centres spread across India
Mr. Ajay Saxena
Ph. 8467888005
Website : www.reelabs.com
Email : ajay.saxena@reelabs.com
Q.& A.session Dr. Sharda Jain
Mr. Ajay Saxena