This document discusses stem cells, their types and sources. It describes that stem cells have the ability to self-renew and differentiate into other cell types. The main stem cell sources discussed are embryonic stem cells, adult stem cells, induced pluripotent stem cells and umbilical cord blood stem cells. Clinical applications of stem cells mentioned include hematopoietic stem cell transplantation to treat blood disorders and bone marrow failure, as well as use in bone grafting, corneal regeneration and tissue engineering.
iPSCs are pluripotent; unlike ESC, iPSCs are not derived from the embryo, but instead created from differentiated cells in the lab through a process – cellular reprogramming.
iPSCs are pluripotent; unlike ESC, iPSCs are not derived from the embryo, but instead created from differentiated cells in the lab through a process – cellular reprogramming.
Imagine that you have been told you have an illness that cannot be cured or what if your body has been irreversibly paralysed. There is no hope. But there is a science that could change that. It’s Called Stem Cell Research and it’s an important step in the medical revolution. But it comes with controversies as it uses Human Embryos’ as Raw Material.
But something astounding happened in the year 2006 that removed the usage of surplus embryos from the equation altogether. It’s about a brand new technology that can turn back the clock on your body cells. This is cutting edge of science where new developments are happing all the time. The iPSCs could be the potential medicine of 21st century. So what are stem cells? Why do they Matter? What are iPSCs and how it changed the biological rules?
A stem cell is a "blank" cell that can give rise to multiple tissue types such as a skin, muscle, or nerve cell.
Under certain physiologic or experimental conditions, they can be induced to become tissue- or organ-specific cells with special functions.
What are stem cells? This presentation provides an overview of multiple different stem cells including embryonic stem cells, mesenchymal stem cells, cancer stem cells, induced pluripotent stem cells, hematopoietic stem cells and neural stem cells.
Stem cells
Undifferentiated cells capable of self-renew and to differentiate into different cell types or tissues during embryonic development and throughout adulthood.
Have possibility to become a specialised cell.
Have the ability to divide continuously and develop into various other kinds of cells.
Have immune potential and can help to treat a wide range of medical problems.
Discovery of stem cells lead to a whole new branch of medicine known as Regenerative medicine.
history ,definition,type of stem cells , characters of stem cells , source, stem cell banking , indications of stem cell therapy ,applications in gynaecology
What is Stem Cell ?
History of Stem Cells ?
Stages of Embryogenesis
Blastocyst Diagram
Three types of stem cells
Differentiation of ESC
Adult Stem Cells
Bone Marrow
Umbilical cord stem cells
Factors known to affect stem cells
Niche cells activates Stem cells
Regenerative Medicine : Indian Scenario
This presentation describes in detail the various types and sources of stem cells. it also describes the stem cell therapies used to treat various diseases.
Imagine that you have been told you have an illness that cannot be cured or what if your body has been irreversibly paralysed. There is no hope. But there is a science that could change that. It’s Called Stem Cell Research and it’s an important step in the medical revolution. But it comes with controversies as it uses Human Embryos’ as Raw Material.
But something astounding happened in the year 2006 that removed the usage of surplus embryos from the equation altogether. It’s about a brand new technology that can turn back the clock on your body cells. This is cutting edge of science where new developments are happing all the time. The iPSCs could be the potential medicine of 21st century. So what are stem cells? Why do they Matter? What are iPSCs and how it changed the biological rules?
A stem cell is a "blank" cell that can give rise to multiple tissue types such as a skin, muscle, or nerve cell.
Under certain physiologic or experimental conditions, they can be induced to become tissue- or organ-specific cells with special functions.
What are stem cells? This presentation provides an overview of multiple different stem cells including embryonic stem cells, mesenchymal stem cells, cancer stem cells, induced pluripotent stem cells, hematopoietic stem cells and neural stem cells.
Stem cells
Undifferentiated cells capable of self-renew and to differentiate into different cell types or tissues during embryonic development and throughout adulthood.
Have possibility to become a specialised cell.
Have the ability to divide continuously and develop into various other kinds of cells.
Have immune potential and can help to treat a wide range of medical problems.
Discovery of stem cells lead to a whole new branch of medicine known as Regenerative medicine.
history ,definition,type of stem cells , characters of stem cells , source, stem cell banking , indications of stem cell therapy ,applications in gynaecology
What is Stem Cell ?
History of Stem Cells ?
Stages of Embryogenesis
Blastocyst Diagram
Three types of stem cells
Differentiation of ESC
Adult Stem Cells
Bone Marrow
Umbilical cord stem cells
Factors known to affect stem cells
Niche cells activates Stem cells
Regenerative Medicine : Indian Scenario
This presentation describes in detail the various types and sources of stem cells. it also describes the stem cell therapies used to treat various diseases.
This presentation deals with stem cell therapy & new avenues in stem cell therapy. It also discusses latest advances such as treatment against baldness, multiple sclerosis, type 1 diabetes, spinal cord injury, demyelinating diseases, deafness, eye, Parkinson's disease. Also discusses about umbilical cord stem cells and finally clinical trials without patients (organs on chips).
Stem cell technology | Presented by pranjali V. Bhadanepranjali bhadane
This presentation is related to biological topic stem cell technology. stem cell technology plays an important role in life science. stem cell technology is most important for future researchers on personal genomics, personal drug and Complex Disease Research...
Flu Vaccine Alert in Bangalore Karnatakaaddon Scans
As flu season approaches, health officials in Bangalore, Karnataka, are urging residents to get their flu vaccinations. The seasonal flu, while common, can lead to severe health complications, particularly for vulnerable populations such as young children, the elderly, and those with underlying health conditions.
Dr. Vidisha Kumari, a leading epidemiologist in Bangalore, emphasizes the importance of getting vaccinated. "The flu vaccine is our best defense against the influenza virus. It not only protects individuals but also helps prevent the spread of the virus in our communities," he says.
This year, the flu season is expected to coincide with a potential increase in other respiratory illnesses. The Karnataka Health Department has launched an awareness campaign highlighting the significance of flu vaccinations. They have set up multiple vaccination centers across Bangalore, making it convenient for residents to receive their shots.
To encourage widespread vaccination, the government is also collaborating with local schools, workplaces, and community centers to facilitate vaccination drives. Special attention is being given to ensuring that the vaccine is accessible to all, including marginalized communities who may have limited access to healthcare.
Residents are reminded that the flu vaccine is safe and effective. Common side effects are mild and may include soreness at the injection site, mild fever, or muscle aches. These side effects are generally short-lived and far less severe than the flu itself.
Healthcare providers are also stressing the importance of continuing COVID-19 precautions. Wearing masks, practicing good hand hygiene, and maintaining social distancing are still crucial, especially in crowded places.
Protect yourself and your loved ones by getting vaccinated. Together, we can help keep Bangalore healthy and safe this flu season. For more information on vaccination centers and schedules, residents can visit the Karnataka Health Department’s official website or follow their social media pages.
Stay informed, stay safe, and get your flu shot today!
The prostate is an exocrine gland of the male mammalian reproductive system
It is a walnut-sized gland that forms part of the male reproductive system and is located in front of the rectum and just below the urinary bladder
Function is to store and secrete a clear, slightly alkaline fluid that constitutes 10-30% of the volume of the seminal fluid that along with the spermatozoa, constitutes semen
A healthy human prostate measures (4cm-vertical, by 3cm-horizontal, 2cm ant-post ).
It surrounds the urethra just below the urinary bladder. It has anterior, median, posterior and two lateral lobes
It’s work is regulated by androgens which are responsible for male sex characteristics
Generalised disease of the prostate due to hormonal derangement which leads to non malignant enlargement of the gland (increase in the number of epithelial cells and stromal tissue)to cause compression of the urethra leading to symptoms (LUTS
Tom Selleck Health: A Comprehensive Look at the Iconic Actor’s Wellness Journeygreendigital
Tom Selleck, an enduring figure in Hollywood. has captivated audiences for decades with his rugged charm, iconic moustache. and memorable roles in television and film. From his breakout role as Thomas Magnum in Magnum P.I. to his current portrayal of Frank Reagan in Blue Bloods. Selleck's career has spanned over 50 years. But beyond his professional achievements. fans have often been curious about Tom Selleck Health. especially as he has aged in the public eye.
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Introduction
Many have been interested in Tom Selleck health. not only because of his enduring presence on screen but also because of the challenges. and lifestyle choices he has faced and made over the years. This article delves into the various aspects of Tom Selleck health. exploring his fitness regimen, diet, mental health. and the challenges he has encountered as he ages. We'll look at how he maintains his well-being. the health issues he has faced, and his approach to ageing .
Early Life and Career
Childhood and Athletic Beginnings
Tom Selleck was born on January 29, 1945, in Detroit, Michigan, and grew up in Sherman Oaks, California. From an early age, he was involved in sports, particularly basketball. which played a significant role in his physical development. His athletic pursuits continued into college. where he attended the University of Southern California (USC) on a basketball scholarship. This early involvement in sports laid a strong foundation for his physical health and disciplined lifestyle.
Transition to Acting
Selleck's transition from an athlete to an actor came with its physical demands. His first significant role in "Magnum P.I." required him to perform various stunts and maintain a fit appearance. This role, which he played from 1980 to 1988. necessitated a rigorous fitness routine to meet the show's demands. setting the stage for his long-term commitment to health and wellness.
Fitness Regimen
Workout Routine
Tom Selleck health and fitness regimen has evolved. adapting to his changing roles and age. During his "Magnum, P.I." days. Selleck's workouts were intense and focused on building and maintaining muscle mass. His routine included weightlifting, cardiovascular exercises. and specific training for the stunts he performed on the show.
Selleck adjusted his fitness routine as he aged to suit his body's needs. Today, his workouts focus on maintaining flexibility, strength, and cardiovascular health. He incorporates low-impact exercises such as swimming, walking, and light weightlifting. This balanced approach helps him stay fit without putting undue strain on his joints and muscles.
Importance of Flexibility and Mobility
In recent years, Selleck has emphasized the importance of flexibility and mobility in his fitness regimen. Understanding the natural decline in muscle mass and joint flexibility with age. he includes stretching and yoga in his routine. These practices help prevent injuries, improve posture, and maintain mobilit
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Lung Cancer: Artificial Intelligence, Synergetics, Complex System Analysis, S...Oleg Kshivets
RESULTS: Overall life span (LS) was 2252.1±1742.5 days and cumulative 5-year survival (5YS) reached 73.2%, 10 years – 64.8%, 20 years – 42.5%. 513 LCP lived more than 5 years (LS=3124.6±1525.6 days), 148 LCP – more than 10 years (LS=5054.4±1504.1 days).199 LCP died because of LC (LS=562.7±374.5 days). 5YS of LCP after bi/lobectomies was significantly superior in comparison with LCP after pneumonectomies (78.1% vs.63.7%, P=0.00001 by log-rank test). AT significantly improved 5YS (66.3% vs. 34.8%) (P=0.00000 by log-rank test) only for LCP with N1-2. Cox modeling displayed that 5YS of LCP significantly depended on: phase transition (PT) early-invasive LC in terms of synergetics, PT N0—N12, cell ratio factors (ratio between cancer cells- CC and blood cells subpopulations), G1-3, histology, glucose, AT, blood cell circuit, prothrombin index, heparin tolerance, recalcification time (P=0.000-0.038). Neural networks, genetic algorithm selection and bootstrap simulation revealed relationships between 5YS and PT early-invasive LC (rank=1), PT N0—N12 (rank=2), thrombocytes/CC (3), erythrocytes/CC (4), eosinophils/CC (5), healthy cells/CC (6), lymphocytes/CC (7), segmented neutrophils/CC (8), stick neutrophils/CC (9), monocytes/CC (10); leucocytes/CC (11). Correct prediction of 5YS was 100% by neural networks computing (area under ROC curve=1.0; error=0.0).
CONCLUSIONS: 5YS of LCP after radical procedures significantly depended on: 1) PT early-invasive cancer; 2) PT N0--N12; 3) cell ratio factors; 4) blood cell circuit; 5) biochemical factors; 6) hemostasis system; 7) AT; 8) LC characteristics; 9) LC cell dynamics; 10) surgery type: lobectomy/pneumonectomy; 11) anthropometric data. Optimal diagnosis and treatment strategies for LC are: 1) screening and early detection of LC; 2) availability of experienced thoracic surgeons because of complexity of radical procedures; 3) aggressive en block surgery and adequate lymph node dissection for completeness; 4) precise prediction; 5) adjuvant chemoimmunoradiotherapy for LCP with unfavorable prognosis.
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ARTIFICIAL INTELLIGENCE IN HEALTHCARE.pdfAnujkumaranit
Artificial intelligence (AI) refers to the simulation of human intelligence processes by machines, especially computer systems. It encompasses tasks such as learning, reasoning, problem-solving, perception, and language understanding. AI technologies are revolutionizing various fields, from healthcare to finance, by enabling machines to perform tasks that typically require human intelligence.
Couples presenting to the infertility clinic- Do they really have infertility...Sujoy Dasgupta
Dr Sujoy Dasgupta presented the study on "Couples presenting to the infertility clinic- Do they really have infertility? – The unexplored stories of non-consummation" in the 13th Congress of the Asia Pacific Initiative on Reproduction (ASPIRE 2024) at Manila on 24 May, 2024.
Anti ulcer drugs and their Advance pharmacology ||
Anti-ulcer drugs are medications used to prevent and treat ulcers in the stomach and upper part of the small intestine (duodenal ulcers). These ulcers are often caused by an imbalance between stomach acid and the mucosal lining, which protects the stomach lining.
||Scope: Overview of various classes of anti-ulcer drugs, their mechanisms of action, indications, side effects, and clinical considerations.
How to Give Better Lectures: Some Tips for Doctors
Stem cells biology and their application in clinical medicine
1. STEM CELLS BIOLOGY AND THEIR
APPLICATION IN CLINICAL MEDICINE
Presented by:
Dr. Rajesh Shukla
DNB Internal Medicine Resident
Chinmaya Mission Hospital
Bangalore.
2. STEM CELL
• Stem cells are those cells that have the
capability of self-renewal and differentiation.
• Self-renewal is the ability of cells to proliferate
without the loss of differentiation potential
and without undergoing senescence (biologic
aging).
3.
4. The evolving role of stem cells in clinical medicine is
developing along at least three lines:
●Stem cells as therapy (either to replace cell lines
that have been lost or destroyed, or to modify the
behavior of other cells)
●Stem cells as targets of drug therapy
●Stem cells to generate differentiated tissue for in
vitro study of disease models for drug
development.
5. • The potency of a stem cell is defined by the
types of more differentiated cells that the
stem cell can make. Stem cells can be either:
• totipotent,
• pluripotent,
• multipotent, or
• unipotent.
6. • Totipotent cells have the capability to produce all cell types
of the developing organism, including both embryonic and
extraembryonic (eg, placenta) tissues.
• Pluripotent cells can only make cells of the embryo proper,
but make all cells of the embryo including germ cells and
cells from any of the germ layers. Therefore, they can make
any cell of the body.
• Multipotent cells can only make cells within a given germ
layer. For example, multipotent stem cells from a
mesodermal tissue like the blood can make all the cells of
the blood, but cannot make cells of a different germ layer
such as neural cells (ectoderm) or liver cells (endoderm).
• Unipotent cells make cells of a single cell type. An example
is a germ cell stem cell that makes the cells that mature to
become egg or sperm, but not other cell types.
7. • Totipotent Cells-- first few cell divisions
following fertilization of the egg.
• Pluripotent Cells-- cells derived from the inner
cell mass of the blastocyst (a pre-implantation
stage of development occurring approximately
7 to 10 days after fertilization).
• Multipotent or Unipotent Cells-- primitive
streak forms in embryonic development (day
10 to 14 post fertilization)
8. Embryonic stem (ES) cells
• Embryonic stem cells are typically derived
from a preimplantation blastocyst (7 to 10
days post fertilization).
• The most frequent techniques used to derive
ES cells disrupt the blastocyst from which the
cells are derived.
9.
10. Adult stem cells
• Present in most, but not all, tissues and persist
throughout life.
• Basis for tissue maintenance and response to
injury.
• Tissues where there is high cell turnover, such
as the blood, skin, and intestine.
• Resident stem cell population for some tissues
where the rate of cell turnover is lower (such
as muscle, brain, lung, and kidney)
12. Induced pluripotent stem (iPS) cells
• The concept of the close relationship between stem
cell potential and stage of development dramatically
changed in 2006
14. • Shinya Yamanaka and his colleagues took genes
that were expressed in pluripotent ES cells, but not
generally in mature cells, and introduced them into
mature cells.
• They did so in a manner such that the genes would
now be “ectopically” expressed, ie, expressed in a
cell type where the gene is normally not expressed.
• A small number of the mature cells reverted back
to a highly immature cell state that resembled an
ES cell.
• This process, now called reprogramming, induced
a pluripotent state in a previously differentiated
cell type .These cells are therefore called induced
pluripotent stem (iPS) cells.
15. Ability to induce pluripotency has changed the
landscape of stem cell biology
• ●First, it indicates that the state of a given cell (ie,
its level of differentiation) can be manipulated,
resulting in drastic shifts in cell function. Cells
have a plasticity that is far greater than previously
recognized and can be programmed by specific
manipulations to achieve a different cell state. A
keratinocyte derived from the skin can be
induced to become a pluripotent stem cell,
essentially rewinding the cell’s history to revert to
an embryonic-like state.
16. • ●Second, a cell taken from an individual can be
induced to become a cell type capable of forming
any other cell type in that individual's body. This
means, for example, that a skin or blood sample
obtained from a patient with a degenerative
brain disorder can be converted into a
pluripotent cell. The iPS cell can then become a
source for generating the neural cells affected by
the disease. Thus, disease models can be
generated using human cells for disorders where
obtaining primary tissue, or developing reliable
animal models, has been difficult.
17. • ●Third, iPS from a given individual represents
a highly personalized source of cells. While
technologies to generate iPS currently involve
genetic manipulation, it is anticipated that
other methods will be developed to generate
iPS that are genetically identical to the
individual. Such cells could be used to assess
'personalized' drug therapies and may
represent a source of immunologically
identical cells for transplantation.
18. Umbilical Cord Stem Cells
• Umbilical cord blood stem/progenitor cells are widely
and easily available, and they appear to be associated
with less graft-versus-host disease than are some other
cell types, such as marrow stem cells.
• Less human leukocyte antigen (HLA) restriction than
adult marrow stem cells and are less likely to be
contaminated with herpesvirus.
• unclear how many different cell types can be generated
• methods for differentiating these cells into
nonhematopoietic phenotypes are largely lacking.
• quantity of obtainable cells may be limiting.
19. SOURCES OF STEM CELLS
• Stem cells can be derived from human
embryos or somatic tissues in the adult or
they can be created by inducing greater
potency in an already differentiated somatic
cell.
• Embryonic stem (ES) cells
• Adult stem cells
• Induced pluripotent stem (iPS) cells
• Umbilical Cord Stem Cells
32. General Strategies for Stem Cell Replacement
1. One therapeutic approach involves direct
administration of stem cells.
This may involve injection of the cells directly into
the damaged organ, where they can differentiate
into the desired cell type.
Alternatively, stem cells may be injected
systemically since they have the capacity to home in
on damaged tissues by following gradients of
cytokines and chemokines released by the diseased
organ.
33. 2. Second approach involves transplantation of
differentiated cells derived from stem cells. For
example, pancreatic islet cells would be
generated from stem cells before
transplantation into diabetic patients and
cardiomyocytes would be generated to treat
ischemic heart disease.
34. • A third approach involves stimulation of endogenous
stem cells to facilitate repair.
• This might be accomplished by administration of
appropriate growth factors and drugs that amplify the
number of endogenous stem/progenitor cells and/or
direct them to differentiate into the desired cell types.
• Therapeutic stimulation of precursor cells is already a
clinical reality in the hematopoietic system, where
factors such as erythropoietin, granulocyte colony-
stimulating factor (G-CSF), and granulocyte-
macrophage colony-stimulating factor (GM-CSF) are
used to increase production of specific blood elements.
35. • Ex vivo or In situ generation of tissues, a
process termed tissue engineering.
• As vehicles for cellular gene therapy.
• Transplanted stem cells may exert paracrine
effects on damaged tissues without
differentiating and replacing lost cells.
36. CLINICAL APPLICATIONS
• In 1963, researchers in Toronto demonstrated
that a bone marrow-derived cell could replace all
the blood elements and rescue an otherwise
lethally-irradiated animal by simple infusion of
donor cells into the blood.
• Over the ensuing twenty years, hematopoietic
stem cell transplantation has become a standard
means of treating individuals with hematologic
malignancies as well as clinical and acquired bone
marrow failure states, including radiation injury.
37. • In 1975, it was reported that cultured cells
from the skin could result in the generation of
large numbers of cells sufficient to provide an
autologous cutaneous barrier in patients with
severe burns
38. • Stem cells are now used for bone grafting in
orthopedics
• Corneal generation from limbal stem cells in
ophthalmology.
39. Hematopoetic Stem cell
Transplant(HSCT)
• Autologous HSCT is used to treat the following
conditions:
Multiple myeloma
Non-Hodgkin lymphoma
Hodgkin disease
Acute myeloid leukemia
Neuroblastoma
Germ cell tumors
Autoimmune disorders (systemic lupus erythematosus
[SLE], systemic sclerosis)
Amyloidosis
40. • Allogenic HSCT is used to treat the following disorders:
• Acute myeloid leukemia
• Acute lymphoblastic leukemia
• Chronic myeloid leukemia
• Chronic lymphocytic leukemia
• Myeloproliferative disorders
• Myelodysplastic syndromes
• Multiple myeloma
• Non-Hodgkin lymphoma
• Hodgkin disease
• Aplastic anemia
• Pure red-cell aplasia
• Paroxysmal nocturnal hemoglobinuria
• Fanconi anemia
• Thalassemia major
• Sickle cell anemia
• Severe combined immunodeficiency (SCID)
• Wiskott-Aldrich syndrome
• Hemophagocytic lymphohistiocytosis
• Inborn errors of metabolism
• Epidermolysis bullosa
• Severe congenital neutropenia
• Shwachman-Diamond syndrome
• Diamond-Blackfan anemia
• Leukocyte adhesion deficiency
41. • Donor types include the following:
Identical twin donors
Matched related donors
Matched unrelated donors
Mismatched related donors
Haploidentical donors
Umbilical cord blood donors
42. • Sources of stem cells are as follows:
• Bone marrow (traditional source)
• Peripheral blood (now preferred for many transplantations)
• Umbilical cord blood
• Procedural considerations
• Preparative or conditioning regimens involve delivery of maximally
tolerated doses of multiple chemotherapeutic agents with
nonoverlapping toxicities and may be classified as follows:
• Myeloablative regimens – These are designed to kill all residual
cancer cells in autologous or allogenic transplantation and to cause
immunosuppression for engraftment in allogenic transplantation;
they may be further subclassified as radiation-containing or non–
radiation-containing
• Nonmyeloablative regimens – These are immunosuppressive but
not myeloablative and rely on the graft-versus-tumor effect to kill
tumor cells with donor T cells
43.
44.
45. Preclinical examples using human ES cells
• Human embryonic stem (ES) cells have been
successfully differentiated in vitro into
multiple cell types for therapeutic uses,
• oligodendrocytes,
• pancreatic cells,
• cardiomyocytes,
• motor and dopaminergic neurons, and
• hematopoietic precursor cells
46. • The therapeutic potential for ES cell-derived
somatic cells has been demonstrated in
animal models of :
Retinal blindness,
Parkinson's disease,
Huntington disease,
Spinal cord injury,
Myocardial infarction, and
Type I diabetes mellitus.
47. Retinal Diseases
• Human ES cell-derived retinal photoreceptors have
been used to improve visual function in blind mice.
• Following intraocular injection, retinal cells derived
from human ES cells migrated into the appropriate
retinal layers and expressed markers of differentiated
rod and cone photoreceptor cells.
• Subretinal transplantation of the cells into the
subretinal space of mice modeling Leber's congenital
amaurosis restored light responses to the animals.
• In a subsequent study, ES cell-derived photoreceptors
transplanted into the eyes of adult mice were able to
integrate and mature into outer segment-bearing
photoreceptors
48. Parkinson disease
• A highly enriched population of midbrain neural
stem cells was derived from mouse ES cells.
• The dopamine neurons generated by these stem
cells showed electrophysiologic and behavioral
properties expected of neurons from the
midbrain.
• These ES-derived neurons survived after being
transplanted into rats with symptoms of
Parkinson disease, showed appropriate
electrophysiological properties, and ameliorated
pathologic movements in the animals.
49. • Subsequently, functional improvement was
seen with use of human ES cell-derived
dopaminergic neurons after transplantation in
a rat model of Parkinsonism.
• Similarly, transplantation of differentiation
stage defined ESC-derived neuronal
progenitors resulted in dopamine neuron
engraftment, with robustly induced recovery
of motor deficits in hemiparkinsonian mice.
50. Huntington Disease
• Degeneration of gamma-aminobutyric acid (GABA)
neurons in the basal ganglia underlies motor
dysfunction in Huntington disease (HD).
• Human ES cells that have been differentiated into
forebrain GABA neurons have been transplanted
into the brains of mice in an HD model.
• These ES-cell-derived neurons were able to
repopulate the substantia nigra, receive
glutamatergic and dopaminergic inputs, and
restore motor neuron function.
51. Spinal Cord Injury
• Transplantation of human ES-derived
oligodendrocyte progenitor cells into adult rats,
seven days after the induction of a spinal cord
injury, was shown to enhance remyelination and
promote improved motor function.
• Transplanted cells survived, redistributed over
short distances, and differentiated into
oligodendrocytes, demonstrating their
therapeutic potential after recent spinal cord
injury.
52. Myocardial Infarction and Heart Failure
• Several studies have demonstrated that transplantation of human
ES-derived cardiomyocytes improves contractile function of the
infarcted mouse heart.
• Using a combination of pro-survival factors that limited
cardiomyocyte death after transplantation, one group
demonstrated decreased rates of heart failure and partial
remuscularization (as evidenced by systolic thickening of the
infarction wall) in animals treated with human ES-derived
cardiomyocytes when compared to control animals treated with
non-cardiac human ES derivatives.
• Similar results in another study highlight the potential of human ES
for myocardial cell therapy strategies.
• Preliminary proof-of-concept studies have tested the safety and
efficacy of various delivery systems for hematopoietic stem cell-
based therapy in patients with ischemic cardiomyopathy.
53. Diabetes Mellitus Type I
• There are currently no reproducible methods for the
efficient direct differentiation of human ES cells to
insulin-producing beta cells, although the generation of
beta cells in vitro by selecting for the expression of
beta cell genes or varying growth conditions has been
reported.
• However, the physiologic ability of human ES-derived
pancreatic endoderm to differentiate in vivo into
glucose-responsive insulin-secreting cells in
immunodeficient mice with streptozotocin-induced
diabetes, ameliorating hyperglycemia in these mice,
has been demonstrated
54. • Clinical use of human ES cells — In 2012, the first report of
the safety and tolerability of human ES cell use for the
treatment of two patients with retinal disease appeared
(one patient with dry age-related macular degeneration,
one patient with Stargardt’s macular dystrophy) .
• Human ES cell differentiation resulted in greater than 99
percent pure retinal pigment epithelium (RPE), of which
50,000 RPE cells were injected into the subretinal space.
• The human ES cell-derived RPE proliferation was confined
to the subretinal space.
• Both patients were treated with low-dose tacrolimus and
mycophenolate mofetil for immunosuppression beginning
one week before the procedure and continuing for six
weeks, followed by six additional weeks of mycophenolate
mofetil only, with no signs of rejection noted during the
follow-up.
• Improvement in visual acuity was noted in both patients
55. Preclinical studies using iPS cells
• iPS cell technology could overcome two important
obstacles associated with human ES cells:
1. immune rejection after transplantation and
2. ethical concerns regarding the use of human
embryos.
The approach would be particularly powerful in
monogenic diseases, where ‘patient-specific’ iPS can be
generated, the diseased gene in those cells potentially
corrected, and the gene-corrected cells transplanted to
restore organ function.
This field is rapidly evolving, although there remains a
great deal of research needed to make such a schema
viable in the clinical setting.
56. • These studies permit testing of basic
principles in a physiologic setting and are
therefore useful, particularly where murine
models of human diseases have been
generated. For two such models, Parkinson
disease and hemophilia A, iPS cells have been
generated and used to differentiate cells that
could then be transplanted for therapy.
57. • Other technologies developed for gene therapy have also been
used to test whether iPS cells can be used to create gene corrected
cells for transplantation.
• One proof-of-principle study used a mouse model of sickle cell
disease in which the mouse hemoglobin genes contained the
mutations known to cause sickling in human sickle cell anemia.
• Investigators generated iPS cells from skin cells of the mouse and
introduced a normal hemoglobin gene allele to replace the sickle
hemoglobin gene (using the gene targeting technology used to
engineer mouse strains for research) .
• They then generated blood stem cells from the gene corrected iPS
cells. Transplantation of these cells into sickle cell anemic mice
improved the disease phenotype. This represents a model for the
use of iPS cells as cell therapy in the setting of genetic disorders.
58. • The approach of using iPS cells to correct a genetic
defect has been tested using human cells.
• Fanconi anemia — A normal version of the gene
mutated in Fanconi anemia was introduced into
fibroblasts from patients with Fanconi anemia, and the
gene-corrected cells were reprogrammed to generate
patient-specific iPS cells [31]. The iPS cells carrying the
normal gene were able to generate blood cell types in
a manner similar to cells from normal volunteers. This
study indicated that skin cells from patients with an
abnormal gene can be used to generate gene-corrected
iPS cells that might serve as a source of cells for
transplantation.
59. • Huntington disease (HD) — The abnormally expanded
cytosine-adenine-guanine (CAG) trinucleotide repeat
region of the huntingtin gene was corrected to normal
length in fibroblasts derived from a patient with HD.
• These fibroblasts were reprogrammed to generate
patient-specific iPS cells, which were then
differentiated into gamma-aminobutyric acid (GABA)-
producing neurons.
• When transplanted into mice, the neurons were able to
populate the basal ganglia and express GABA.
• In cell culture, the neurons showed normalization of
signaling pathways, reduced susceptibility to cell death,
and improved mitochondrial function, all of which are
thought to be involved in the pathogenesis of HD.
60. Challenges in the clinical use of stem cells as
replacement
• Tissue integration
• Oncogenesis
• Directed differentiation
• Uniformity and consistency
61. Tissue integration
• The first issue is how transplanted cells will
integrate into surrounding tissue to achieve a
physiologically beneficial effect. This is of
particular relevance where coordination of
complex networks of cells is essential, such as
in the heart and brain where aberrant circuits
can result in serious adverse events.
62. Oncogenesis
• A second concern is the potential for transplanted cells to form
tumors. This is of particular importance when using pluripotent
cells, since these are characterized by the ability to form teratomas
(neoplastic tumors containing cells corresponding to all three
embryonic layers) in animal models.
• Thus, the differentiation state of transplanted cells will need to be
defined with high precision to avoid delivery of residual pluripotent
cells that may differentiate aberrantly in vivo.
• Evidence also suggests that oncogenesis is not restricted to
pluripotent cells.
• The culture process may enable the outgrowth of genetically
abnormal cell types that could be of potential danger. The specifics
of what types of testing will be required for pluripotent or other
cells to assure genetic integrity of transplanted cells is an active
area of consideration.
63. • The issue of malignant potential of cells is of greatest
concern in induced pluripotent stem cells (iPS) cells.
• The methods of greatest efficiency for reprogramming cells
are currently retrovirus or lentivirus-based, and therefore
run the risk of mutagenesis by virtue of viral integration
into the host genome.
• In addition, some of the genes used to induce
reprogramming have known oncogenic potential (eg, c-
Myc).
• Progress in reducing the number of gene products needed
for reprogramming, and in the use of either non-integrating
viruses or small molecules to supplant retrovirus-based
reprogramming, is ongoing.
• These developments may mitigate the concerns about
insertional mutagenesis, but will not entirely assuage
concern for altered growth control of modified cells,
particularly those with pluripotency.
64. Directed differentiation
• A third concern is the ability to direct the differentiation state of the cells
to be used.
• Generating the proper cell type from pluripotent cells remains a significant
challenge for some cell types.
• Protocols have now been devised to create some neural cell types of clear
clinical importance.
• However, for other tissues, such as the blood, the cell types created most
closely resemble embryonic blood cells and are not capable of engrafting
the bone marrow without further and undesirable genetic manipulation.
• Achieving the right stage of differentiation is another consideration in
development of the stem cell derived cell therapies. It may be most
desirable to generate progenitors, rather than fully mature terminally
differentiated cells in some tissues so that the replaced cells do not
quickly senesce and die.
65. Uniformity and consistency
• Finally, the uniformity and consistency of the
cell product may be difficult to achieve across
different donor sources of cells.
67. Disease modifiers
• Beyond use of stem cells for cell replacement, the ability of certain
stem cells to alter disease without engrafting has been explored as
a means of modifying cellular response to injury or aberrant
immune activity.
• Such non-engrafting stem cells are hypothesized to provide
complex signals, affecting disease outcome without directly
replacing injured cells.
• The identification of a population of cells in the bone marrow that
could form a number of mesenchymal cell populations ex vivo led
to the concept of a mesenchymal stem cell [44,45]. This population
of cells is defined primarily by its ability to form colonies in tissue
culture, and by the ability of single cells derived from the colonies
to differentiate into osteoblasts, adipocytes or chondrocytes in
vitro. Identification of these cells in the body, and understanding of
how they function in vivo, remains controversial.
68. • The potential for these mesenchymal stem cells to form
muscle led to exploration of their use in the setting of
ischemic injury of the heart. While initial indications
suggested that the cells may directly contribute to
generating cells in the area of injury, it has subsequently
been shown that the cells do not engraft.
• Rather, it is proposed that they provide complex signals, in
a paracrine manner, that may alter the ability of the heart
(and other tissues) to respond to ischemic injury .This
concept remains highly contested, in part because the
mechanisms by which stem cells may function in this
capacity are not clear.
• The mesenchymal stem cell populations studied are highly
variable and have been variably reported to secrete a
number of factors including indoleamine 2, 3-dioxygenase,
prostaglandins, interleukin-6, hepatocyte growth factor,
inducible nitric oxide synthase, and tumor growth factor
(TGF)-ß1.
69. • It has been proposed that the mesenchymal stem cell population is
capable of altering immune function and may therefore modulate
injury responses as in ischemia, or mitigate the effects of immune
mediated diseases.
• Mesenchymal stem cells have been tested in clinical trials in
humans in settings of graft versus host disease following allogeneic
hematopoietic stem cell transplantation, and in inflammatory bowel
disease. For each, there has been controversy regarding the impact
of the cells and the mechanism by which the cells may be acting.
• While this use of stem cell populations as disease modifying cell
platforms is an area of active investigation, the concept should not
be considered proven at this time.
• The prospect that mesenchymal cell populations may have the
ability to arrive at sites of injury and provide paracrine signals is an
exciting and potentially important application of stem cell biology,
but it is not yet a defined therapy.
70. Drugs targeting endogenous stem cells
• There is emerging recognition that stem cells resident
in adult tissues may be targets for pharmacologic
interventions with the goal of altering the function of
endogenous stem cells. This approach may avoid
concerns related to tissue integration of exogenous
cells.
• Growing understanding of the signaling processes that
induce activation of stem cells, or modify their
differentiation, makes this approach one of growing
interest. Analogous to the use of erythropoietin to
modify the activity of red cell progenitors, agents with
similar effects on stem cells might generate
reconstitution of other types of blood cells in the case
of hematopoietic stem cells, or other mature cell types
for other tissue stem cells.
71. Disease models
• Stem cells offer the possibility of creating in vitro disease models
that may improve molecular understanding of disease and
accelerate the development of new therapies. Human cells from
affected individuals with diseases affecting the nervous system, for
example, have been extremely difficult to obtain for in vitro
analysis.
• With stem cell approaches, it is possible to generate sufficiently
large numbers of cells to study the molecular basis of functional
deficits associated with the disease or to be used in the
development and testing of new drugs.
• The development of induced pluripotent stem cells (iPS) cells has
led to a number of efforts to create in vitro disease models
amenable to genetic and small molecule study.
• A major goal of such efforts is to identify compounds capable of
altering the progression of cell events corresponding to disease
progression. These efforts depend upon robust cell culture systems
that resemble the in vivo context closely enough to be useful for
drug evaluation. Fulfillment of this requirement still needs
validation.
72. • Two early examples depict use of iPS cells to create a disease model
for further study of human disease pathogenesis and treatment.
1. iPS cell lines were created from patients with familial
dysautonomia (FD), a rare fatal sensory autonomic neuropathy
caused by a point mutation in the IKBKAP gene involved in
transcriptional elongation
2. In another study, iPS cells were generated from skin fibroblasts
from a child with spinal muscular atrophy. These cells expanded
robustly in culture, maintained the disease genotype, and
generated motor neurons that showed selective deficits
compared to those derived from the child's unaffected mother.
• iPS cells have also been used to generate disease models for a wide
variety of (genetic) diseases, including neurological, hematological,
metabolic, and cardiovascular disorders. These studies have
generated novel insights into the molecular mechanisms driving
these disorders and platforms for drug screening.
73. • An additional application of stem cell biology to
disease modeling is in the setting of cancer. The model
hypothesizes that there is a definable population of
malignant cells that can be demonstrated to have the
two cardinal features of stem cells (self-renewal and
differentiation capacity). These cells, like stem cells in
normal tissues, would replace more mature cells
whose lifespan is limited. They are therefore
hypothesized to be the cells that enable persistence
and perhaps metastasis of tumors.
• Studies are now investigating whether drugs affect the
stem-like cells and not just tumor bulk, and whether
agents can target distinctive features of the stem-like
cells of cancer rather than the mixture of cells
comprising a malignancy.
84. ETHICAL CONSIDERATIONS
• The initial, and still most frequent, techniques used to derive ES
cells disrupt the blastocyst from which the cells are derived, raising
concern that an early human life form was being destroyed.
• The source of the blastocyst was generally discarded material from
in vitro fertilization clinics. Nonetheless, the issue of using the
material for research, even if that research was intended to assuage
human suffering, was morally unacceptable for many. These
controversies led to policy decisions among some nations that
precluded ES cell research or, as in the case of the United States,
federal funding to support such research.
• The advent of reprogramming to generate induced pluripotent stem
cells (iPS) has provided an alternative source of pluripotent cells. It
should be recognized that iPS have not yet been shown to be fully
equivalent to ES cells, and ES cells still represent the best defined
pluripotent cell population.
85. • An issue that is relevant for both pluripotent and other stem cells is how
to define safety when proceeding to clinical trial. The area of cell therapies
has been well explored in hematology, transfusion medicine, and
hematopoietic stem cell transplant. Infusion of other cultured cell
products has a well-defined safety profile for keratinocyte skin grafts and
mesenchymal stem cell transplants. Several features are unique to the use
of pluripotent cells, however:
• ●Concern regarding the placement of cells in tissues highly intolerant of
aberrant growth control such as the central nervous system and heart.
• ●Defining the genetic integrity of manipulated cells can be extremely
difficult, though may be resolvable through emerging genomic
technologies.
• ●Whether the cells have tumorigenic potential is hard to define since even
established cancers can be difficult to grow with functional in vitro or in
vivo assays.
• ●Defining the potential to integrate as functional tissue requires in vivo
settings, and animal models have only limited ability to predict events in
humans.
• Therefore, clinical experience will be necessary to define some of the risks
in stem cell-based therapeutics, and careful design of clinical trials with a
range of parameters specific to the field are in process.
86. • Stem cells have enormous potential that is well
recognized by the public, and are a source of
hope for those dealing with otherwise
untreatable medical problems.
• However, the field is in its infancy and progress is
painstaking; for those desperate to get help,
unproven therapies even outside of reputable
centers or clinical trial are a highly tempting
option.
• The potential for patient exploitation raises
another extremely challenging area.
87. • There has been a profusion of centers in many parts of
the world, often offering treatment without clear
definition of what cells would be used, what the source
of cells is, and what the full experience has been.
• Services are often offered for cash payment and with
unclear provisions to monitor safety or respond to
adverse events.
• Patients and providers should ask a range of specific
questions about such therapies. The International
Society for Stem Cell Research (ISSCR) has provided
suggested questions and other information that may
be of use (www.isscr.org).