A mutation is a change in a DNA sequence that can be caused by errors during DNA replication, exposure to mutagens like radiation or chemicals, or infection by viruses. Mutations can be passed on to offspring or occur somatically in body cells. Some mutations can cause genetic disorders like sickle cell anemia, Down syndrome, or Edwards syndrome, while others may have no effect or even provide an advantage to survival. Mutants arise from mutations in existing genomes due to errors in DNA replication or repair.
General method of the preparation of Bacterial Infections, Toxoids, Viral vaccine, Antitoxins, Serum-immune blood derivatives and other products relative to immunity.
Biotechnology with reference to pharmaceutical scienceAdarsh Patil
Introduction: Hisory
Biotechnology Biology + Technology
Defn:-
Any Technological application that uses biological system, living organisms, cells, tissues, explants or derivatives thereof, to make or modify products or process for specific uses.
UNIT 4 Microbial genetics:Transformation,Transduction,Conjugation,Plasmids an...Shyam Bass
(6th Sem B.Pharma Pharmaceutical Biotechnology)
Microbial genetics:
• Transformation,
• Transduction,
• Conjugation,
• Plasmids and transposons,
• Study of the production of - Penicillins, Citric acid, Vitamin B12, Glutamic acid,
Griseofulvin,
• Blood Products: Collection, Processing, and Storage of whole human blood,Dried
human plasma, Plasma substitutes
BY- SHYAM BASS
General method of the preparation of Bacterial Infections, Toxoids, Viral vaccine, Antitoxins, Serum-immune blood derivatives and other products relative to immunity.
Biotechnology with reference to pharmaceutical scienceAdarsh Patil
Introduction: Hisory
Biotechnology Biology + Technology
Defn:-
Any Technological application that uses biological system, living organisms, cells, tissues, explants or derivatives thereof, to make or modify products or process for specific uses.
UNIT 4 Microbial genetics:Transformation,Transduction,Conjugation,Plasmids an...Shyam Bass
(6th Sem B.Pharma Pharmaceutical Biotechnology)
Microbial genetics:
• Transformation,
• Transduction,
• Conjugation,
• Plasmids and transposons,
• Study of the production of - Penicillins, Citric acid, Vitamin B12, Glutamic acid,
Griseofulvin,
• Blood Products: Collection, Processing, and Storage of whole human blood,Dried
human plasma, Plasma substitutes
BY- SHYAM BASS
UNIT 6 Fermentation technology, Fermenters, Study of Media, types of fermenta...Shyam Bass
UNIT-6 6th Sem B.Pharma Pharmaceutical Biotechnology-
Following slides include-
Fermentation technology and biotechnological products :
Fermentation methods and general requirements
Study of media
Equipment
Sterilization methods
Aeration process
Stirring
large scale production fermenter design and its various controls
BY- SHYAM BASS
PREPARATION OF BACTERIAL VACCINES:
Steps involved in killed bacterial vaccine preparation:
1. Selection of an antigen:
The exact strain or strains to be incorporated for preparation of bacterial vaccine.
Eg. Cholera vaccine: smooth strains of the two serological types Inaba and Ogawa
TABC vaccine: O and H antigens in S. typhi and S. paratyphi microorganisms and these organisms also contains Vi antigen.
Each strain is carefully checked for freedom from variation and absence of contaminating organisms.
UNIT-5 Protein Engineering: Brief introduction to protein engineering,Use of ...Shyam Bass
UNIT-5 6th Sem B.PHARMA PHARMACEUTICAL BIOTECHNOLOGY)
Protein Engineering: Brief introduction to protein engineering, Use of microbes in industry, Production of enzymes-general considerations, Amylase, Catalase, peroxidase, Lipase Basic principles of genetic engineering
BY- SHYAM BASS
genetic engineering, principles, b pharma 6th sem, biotechnology
What is a gene ?
Definition
History
Process
Molecular tools of genetic engineering
Restriction enzymes
History of restriction enzyme
Mechanism of action
Types of restriction enzymes
Application of restriction enzymes
Blunt ends
Sticky ends
transgenic
cisgenic.
knockout organism.
Host organism vector
TRANSGENIC PLANTS
DOLLY THE SHIP
TRANSGENIC ANIMALS
Regulations in India (ASU DTAB, ASU DCC), Regulation of
manufacture of ASU drugs - Schedule Z of Drugs & Cosmetics Act for ASU drugs.
Introduction
Regulatory Requirements
Key function of regulatory agencies
Regulation in India
DRUG TECHNICAL ADVISORY BOARD
Drugs Consultative committee-DCC
Schedule Z of Drugs & Cosmetics Act for ASU drugs.
UNIT-1 Introduction to biotechnology and enzyme immobilisation Brief introduc...Shyam Bass
(6th Sem B.Pharma Pharmaceutical Biotechnology)
UNIT-1 Introduction to biotechnology and enzyme immobilization Brief introduction to biotechnology, Enzyme biotechnology- methods of enzyme immobilization and applications, biosensors- working and applications of biosensors in pharmaceutical industries
UNIT 6 Fermentation technology, Fermenters, Study of Media, types of fermenta...Shyam Bass
UNIT-6 6th Sem B.Pharma Pharmaceutical Biotechnology-
Following slides include-
Fermentation technology and biotechnological products :
Fermentation methods and general requirements
Study of media
Equipment
Sterilization methods
Aeration process
Stirring
large scale production fermenter design and its various controls
BY- SHYAM BASS
PREPARATION OF BACTERIAL VACCINES:
Steps involved in killed bacterial vaccine preparation:
1. Selection of an antigen:
The exact strain or strains to be incorporated for preparation of bacterial vaccine.
Eg. Cholera vaccine: smooth strains of the two serological types Inaba and Ogawa
TABC vaccine: O and H antigens in S. typhi and S. paratyphi microorganisms and these organisms also contains Vi antigen.
Each strain is carefully checked for freedom from variation and absence of contaminating organisms.
UNIT-5 Protein Engineering: Brief introduction to protein engineering,Use of ...Shyam Bass
UNIT-5 6th Sem B.PHARMA PHARMACEUTICAL BIOTECHNOLOGY)
Protein Engineering: Brief introduction to protein engineering, Use of microbes in industry, Production of enzymes-general considerations, Amylase, Catalase, peroxidase, Lipase Basic principles of genetic engineering
BY- SHYAM BASS
genetic engineering, principles, b pharma 6th sem, biotechnology
What is a gene ?
Definition
History
Process
Molecular tools of genetic engineering
Restriction enzymes
History of restriction enzyme
Mechanism of action
Types of restriction enzymes
Application of restriction enzymes
Blunt ends
Sticky ends
transgenic
cisgenic.
knockout organism.
Host organism vector
TRANSGENIC PLANTS
DOLLY THE SHIP
TRANSGENIC ANIMALS
Regulations in India (ASU DTAB, ASU DCC), Regulation of
manufacture of ASU drugs - Schedule Z of Drugs & Cosmetics Act for ASU drugs.
Introduction
Regulatory Requirements
Key function of regulatory agencies
Regulation in India
DRUG TECHNICAL ADVISORY BOARD
Drugs Consultative committee-DCC
Schedule Z of Drugs & Cosmetics Act for ASU drugs.
UNIT-1 Introduction to biotechnology and enzyme immobilisation Brief introduc...Shyam Bass
(6th Sem B.Pharma Pharmaceutical Biotechnology)
UNIT-1 Introduction to biotechnology and enzyme immobilization Brief introduction to biotechnology, Enzyme biotechnology- methods of enzyme immobilization and applications, biosensors- working and applications of biosensors in pharmaceutical industries
Deals with various methods adapted for the Improvement in Microbial Cell Culture alongwith the procedure and instruments used to carry out the operation through illustrative diagrams
Global Medical Cures™ | Handbook- Genetic Mutations & How it Impacts HealthGlobal Medical Cures™
Global Medical Cures™ | Handbook- Genetic Mutations & How it Impacts Health
DISCLAIMER-
Global Medical Cures™ does not offer any medical advice, diagnosis, treatment or recommendations. Only your healthcare provider/physician can offer you information and recommendations for you to decide about your healthcare choices.
An overview on mutation, the general mechanisms, classification based on various characteristics, analogy sentence and genetic disorder of various types based on its classification, a brief description of mutagens agents and consequences of mutation in our body and on other living creatures
genetic variations and its role in health/ pharmacologysrivani mandaloju
Here is the reference for the above topic. I have collected the maximum information that i got from the internet. If any one need the complete information comment here.
Institution's Innovation Council (IIC) of Noida Institute of Engineering and Technology (Pharmacy Institute) organized a webinar on "Career Talk: How to ace summer internship?" keynote lecture was delivered by Abhijit Debnath, Asst. Professor NIET (Pharmacy Institute).
Hands-On Training on “Screening of Natural Compounds by Molecular Docking, A...Theabhi.in
Invited Lecture on “Screening of Natural Compounds by Molecular Docking, ADME, Toxicity Studies: Hands-On Training,” Organized by BACS, University of Delhi
Drosophila Model for toxicity Assessment of Nanoparticle based Drug Developme...Theabhi.in
Short Term Training Course on DROSOPHILA BIOLOGY AND NANOBIOTECHNOLOGY (STTC Programme for Mid Career Scientist/UG & PG Teaching Faculty) | 6-20 April 2021
Introduction to various Instructional tools for HEIs by Abhijit DebnathTheabhi.in
Invited talk on One Week Online Faculty Development Programme on "Enhancing Pedagogy through Innovative Instructional Tools for HEIs" 18-24th December 2023
Organized by Faculty of Humanities & Social Sciences, Assam down town University
Video Lecture is available at https://www.youtube.com/watch?v=DXu_CLgB4q0
Introduction, terminology/definitions and rationale, advantages, disadvantages, selection of drug candidates. Approaches to design-controlled release formulations based on diffusion, dissolution and ion exchange principles. Physicochemical and
biological properties of drugs relevant to controlled release formulations.
Introduction to Novel Drug Delivery SystemsTheabhi.in
Full lecture is available in youtube
Part1: https://youtu.be/-8v5VmM-kVg
Part2: https://youtu.be/TdrEUEDAcbU
The Lecture includes:
Introduction to Novel Drug Delivery Systems
What is Drug?
Drug Discovery and Drug Development
Challenges in Drug Discovery & Drug Development
Novel Drug Delivery Systems
Conventional vs Novel Drug Delivery Systems
Various Novel Drug Delivery Systems
NDDS Course contents are available at https://theabhi.in/pharma/ndds
Unit 4 - Informatics & Methods in drug design: Introduction to Bioinformatics, chemoinformatics. ADME databases, chemical, biochemical and pharmaceutical databases.
CADD UNIT V - Molecular Modeling: Introduction to molecular mechanics and quantum mechanics.Energy Minimization methods and Conformational Analysis, global conformational minima determination.
We all have good and bad thoughts from time to time and situation to situation. We are bombarded daily with spiraling thoughts(both negative and positive) creating all-consuming feel , making us difficult to manage with associated suffering. Good thoughts are like our Mob Signal (Positive thought) amidst noise(negative thought) in the atmosphere. Negative thoughts like noise outweigh positive thoughts. These thoughts often create unwanted confusion, trouble, stress and frustration in our mind as well as chaos in our physical world. Negative thoughts are also known as “distorted thinking”.
Instructions for Submissions thorugh G- Classroom.pptxJheel Barad
This presentation provides a briefing on how to upload submissions and documents in Google Classroom. It was prepared as part of an orientation for new Sainik School in-service teacher trainees. As a training officer, my goal is to ensure that you are comfortable and proficient with this essential tool for managing assignments and fostering student engagement.
Read| The latest issue of The Challenger is here! We are thrilled to announce that our school paper has qualified for the NATIONAL SCHOOLS PRESS CONFERENCE (NSPC) 2024. Thank you for your unwavering support and trust. Dive into the stories that made us stand out!
How to Split Bills in the Odoo 17 POS ModuleCeline George
Bills have a main role in point of sale procedure. It will help to track sales, handling payments and giving receipts to customers. Bill splitting also has an important role in POS. For example, If some friends come together for dinner and if they want to divide the bill then it is possible by POS bill splitting. This slide will show how to split bills in odoo 17 POS.
Palestine last event orientationfvgnh .pptxRaedMohamed3
An EFL lesson about the current events in Palestine. It is intended to be for intermediate students who wish to increase their listening skills through a short lesson in power point.
Welcome to TechSoup New Member Orientation and Q&A (May 2024).pdfTechSoup
In this webinar you will learn how your organization can access TechSoup's wide variety of product discount and donation programs. From hardware to software, we'll give you a tour of the tools available to help your nonprofit with productivity, collaboration, financial management, donor tracking, security, and more.
Unit 8 - Information and Communication Technology (Paper I).pdfThiyagu K
This slides describes the basic concepts of ICT, basics of Email, Emerging Technology and Digital Initiatives in Education. This presentations aligns with the UGC Paper I syllabus.
Ethnobotany and Ethnopharmacology:
Ethnobotany in herbal drug evaluation,
Impact of Ethnobotany in traditional medicine,
New development in herbals,
Bio-prospecting tools for drug discovery,
Role of Ethnopharmacology in drug evaluation,
Reverse Pharmacology.
The Indian economy is classified into different sectors to simplify the analysis and understanding of economic activities. For Class 10, it's essential to grasp the sectors of the Indian economy, understand their characteristics, and recognize their importance. This guide will provide detailed notes on the Sectors of the Indian Economy Class 10, using specific long-tail keywords to enhance comprehension.
For more information, visit-www.vavaclasses.com
Students, digital devices and success - Andreas Schleicher - 27 May 2024..pptxEduSkills OECD
Andreas Schleicher presents at the OECD webinar ‘Digital devices in schools: detrimental distraction or secret to success?’ on 27 May 2024. The presentation was based on findings from PISA 2022 results and the webinar helped launch the PISA in Focus ‘Managing screen time: How to protect and equip students against distraction’ https://www.oecd-ilibrary.org/education/managing-screen-time_7c225af4-en and the OECD Education Policy Perspective ‘Students, digital devices and success’ can be found here - https://oe.cd/il/5yV
Operation “Blue Star” is the only event in the history of Independent India where the state went into war with its own people. Even after about 40 years it is not clear if it was culmination of states anger over people of the region, a political game of power or start of dictatorial chapter in the democratic setup.
The people of Punjab felt alienated from main stream due to denial of their just demands during a long democratic struggle since independence. As it happen all over the word, it led to militant struggle with great loss of lives of military, police and civilian personnel. Killing of Indira Gandhi and massacre of innocent Sikhs in Delhi and other India cities was also associated with this movement.
1. Mutation
16 July 2021 Abhijit Debnath BP605T and Biotech Unit-1 1
CO1.1
Noida Institute of Engineering and Technology
(Pharmacy Institute) Greater Noida
Abhijit Debnath
Asst. Professor
NIET, Pharmacy
Institute
Unit: 4
Subject Name: Biotechnology
(BP605T)
Course Details
(B. Pharm 6th Sem)
2. Mutation
16 July 2021 Abhijit Debnath BP605T and Biotech Unit-1 2
Mutation
Types of Mutation
Diseases Caused By Mutation
The Causes of Mutations
Mutants Arise by Mutation
CO1.1
Noida Institute of Engineering and Technology
(Pharmacy Institute) Greater Noida
3. A mutation is a change in a DNA sequence. Mutations can result from DNA copying mistakes made during cell division,
exposure to ionizing radiation, exposure to chemicals called mutagens, or infection by viruses. Germ line mutations occur
in the eggs and sperm and can be passed on to offspring, while somatic mutations occur in body cells and are not passed
on.
MUTATION CO4.2
16 July 2021 Abhijit Debnath BP605T and Biotech Unit-4 3
4. Mutations can also occur as the result of exposure to environmental factors such as smoking, sunlight and radiation.
Often cells can recognise any potentially mutation-causing damage and repair it before it becomes a fixed mutation.
Mutations can also be inherited, particularly if they have a positive effect.
For example, the disorder sickle cell anaemia is caused by a mutation in the gene that instructs the building of a
protein called haemoglobin. This causes the red blood cells to become an abnormal, rigid, sickle shape. However, in
African populations, having this mutation also protects against malaria.
However, mutation can also disrupt normal gene activity and cause diseases, like cancer
Cancer is the most common human genetic disease; it is caused by mutations occurring in a number of growth-
controlling genes. Sometimes faulty, cancer-causing genes can exist from birth, increasing a person’s chance of getting
cancer.
MUTATION CO4.2
16 July 2021 Abhijit Debnath BP605T and Biotech Unit-4 4
6. TYPES OF MUTATION CO4.2
P – Point mutation, or any insertion/deletion entirely inside one gene
D – Deletion of a gene or genes
C – Whole chromosome extra, missing, or both
T – Trinucleotide repeat disorders: gene is extended in length
9. • Sickle cell disease is a group of disorders that
affects hemoglobin, the molecule in red blood
cells that delivers oxygen to cells throughout the
body.
• People with this disease have atypical
hemoglobin molecules called hemoglobin S,
which can distort red blood cells into a sickle, or
crescent, shape.
SICKLE CELL ANEMIA CO4.2
16 July 2021 Abhijit Debnath BP605T and Biotech Unit-4 9
10. SICKLE CELL ANEMIA CO4.2
16 July 2021 Abhijit Debnath BP605T and Biotech Unit-4 10
11. SICKLE CELL ANEMIA CO4.2
16 July 2021 Abhijit Debnath BP605T and Biotech Unit-4 11
12. • Also known as trisomy 21, is a genetic disorder caused by the presence of all or part of a third copy of chromosome
21.
• It is typically associated with physical growth delays, characteristic facial features and mild to moderate intellectual
disability.
• The average IQ of a young adult with Down syndrome is 50, equivalent to the mental age of an 8 or 9 year old child,
but this varies widely.
DOWN SYNDROME CO4.2
16 July 2021 Abhijit Debnath BP605T and Biotech Unit-4 12
13. • Chromosomal disorder caused by the presence of all or part of an extra 18th chromosome.
• This genetic condition almost always results from nondisjunction during meiosis.
• After John Hilton Edwards, who first described the syndrome in 1960.
EDWARD’S SYNDROME CO4.2
16 July 2021 Abhijit Debnath BP605T and Biotech Unit-4 13
14. • Monosomy 7 is typically characterized by early- childhood onset of evidence of
bonemarrow insufficiency/failure associated with increased risk for myelodysplastic
syndrome (MDS) or acute myelogenous leukaemia (AML).
MONOSOMY 7 CO4.2
16 July 2021 Abhijit Debnath BP605T and Biotech Unit-4 14
15. THE CAUSES OF MUTATIONS CO4.2
16 July 2021 Abhijit Debnath BP605T and Biotech Unit-4 15
Four classes of mutations are
(1) spontaneous mutations (molecular decay),
(2) mutations due to error-prone replication bypass of naturally occurring DNA damage (also called error-
prone translesion synthesis),
(3) errors introduced during DNA repair, and
(4) induced mutations caused by mutagens.
16. THE CAUSES OF MUTATIONS CO4.2
16 July 2021 Abhijit Debnath BP605T and Biotech Unit-4 16
Scientists may also deliberately introduce mutant sequences through DNA manipulation for the sake of scientific
experimentation.
One 2017 study claimed that 66% of cancer-causing mutations are random, 29% are due to the environment (the
studied population spanned 69 countries), and 5% are inherited.
Humans on average pass 60 new mutations to their children but fathers pass more mutations depending on their
age with every year adding two new mutations to a child.
17. MUTANT CO4.2
16 July 2021 Abhijit Debnath BP605T and Biotech Unit-4 17
A mutant is an organism or a new genetic character arising or resulting from an instance of mutation, which is generally
an alteration of the DNA sequence of the genome or chromosome of an organism.
It is a characteristic that would not be observed naturally in a specimen.
The term mutant is also applied to a virus with an alteration in its nucleotide sequence whose genome’s in the nuclear
genome.
The natural occurrence of genetic mutations is integral to the process of evolution.
Although not all mutations have a noticeable phenotypic effect, the common usage of the word "mutant" is generally a
pejorative term, only used for genetically or phenotypically noticeable mutations.
Previously, people used the word "sport" (related to spurt) to refer to abnormal specimens. The scientific usage is
broader, referring to any organism differing from the wild type.
18. MUTANTS ARISE BY MUTATION CO4.2
16 July 2021 Abhijit Debnath BP605T and Biotech Unit-4 18
Mutants arise by mutations occurring in pre-existing genomes as a result of errors of DNA replication or errors of DNA
repair.
Errors of replication often involve translesion synthesis by a DNA polymerase when it encounters and bypasses a
damaged base in the template strand.
A DNA damage is an abnormal chemical structure in DNA, such as a strand break or an oxidized base, whereas a
mutation, by contrast, is a change in the sequence of standard base pairs.
Errors of repair occur when repair processes inaccurately replace a damaged DNA sequence. The DNA repair process
microhomology-mediated end joining is particularly error-prone.